LOCUS       pBW010                  4046 bp ds-DNA     circular     27-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(2447..3129)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    63..68
                     /label="mutated BbsI site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Promoter        1514..1548
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Promoter region 77..95
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter region 119..124
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        77..150
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     RBS             151..180
                     /label="sRBS (2466.11 AU)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      2210..2314
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     Promoter region 131..131
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    292..293
                     /label="R1-L"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             1549..1582
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    1514..1519
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    182..1318
                     /label="Fd ER 35"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1583..2209
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    292..1123
                     /label="Trans-ER LBD"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Promoter region 96..101
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    287..291
                     /label="5 bp repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1122..1123)
                     /label="R1-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter region 102..120
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1549..1549
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1537..1542
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    211..211
                     /label="(t) to remove secondary structure"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(3256..3915)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     terminator      3134..3239
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1340..1468
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
ORIGIN
        1 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
       61 TCGTCTTGAC CTCGAGTCCC TATCAGTGAT AGAGATTGAC ATCCCTATCA GTGATAGAGA
      121 TACTGAGCAC ATCAGCAGGA CGCACTGACC TAAAAATCCG ATCCCTCTTT AGTTCTCAAT
      181 CATGGCAACC TATAAAGTTA CACTTATTAA CGAAGCTGAA GGTCTAAACA AAACAATTGA
      241 AGTTCCAGAT GATCAGTACA TTTTAGATGC GGCTGAAGAA GCTGGTATAG ATGCTGCAGC
      301 TGGGGGCGGA GGTTCTGGAG GTGGTGGGTC CAAGAAGAAT TCACTGGCGT TGTCGCTTAC
      361 GGCTGACCAA ATGGTTTCTG CTCTGCTTGA CGCCGAACCT CCGATCCTGT ACTCTGAATA
      421 CGACCCGACT CGTCCTTTTT CAGAGGCGAG TATGATGGGG CTGCTTACGA ATCTGGCAGA
      481 CCGCGAGTTG GTACATATGA TTAATTGGGC TAAACGTGTG CCGGGTTTCG TGGACTTGAC
      541 CCTGCATGAC CAGGTCCACC TGTTAGAATG CGCCTGGTTA GAGATTTTAA TGATCGGACT
      601 TGTCTGGCGT AGCATGGAAC ACCCCGGAAA ACTGTTATTT GCCCCTAACT TGCTGCTGGA
      661 CCGCAACCAG GGGAAGTGTG TCGAAGGAAT GGTGGAAATT TTCGACATGC TTCTTGCCAC
      721 TTCCTCGCGT TTTCGTATGA TGAATTTACA GGGAGAAGAG TTCGTTTGTT TAAAGTCAAT
      781 CATCTTACTG AATAGCGGGG TTTACACATT CTTATCTTCC ACTCTTAAAT CACTGGAGGA
      841 GAAAGACCAT ATTCACCGTG TCCTGGACAA GATCACAGAT ACTCTTATCC ATCTTATGGC
      901 GAAGGCAGGC TTAACACTTC AACAGCAACA TCAGCGCCTT GCTCAGCTTC TGTTGATTTT
      961 AAGCCATATC CGTCACATGT CAAACAAGGG TATGGAACAC CTTTACAGTA TGAAGTGTAA
     1021 GAACGTCGTT CCATTGTATG ATTTGTTACT GGAAATGCTT GACGCTCACC GTCTGCATGC
     1081 ACCTGGTGGC GGTGGCTCTG GTGGAGGCGG GAGCGCAGCT gCAATAGACT TACCGTATTC
     1141 TTGTCGTGCT GGTGCTTGCT CTACCTGTGC TGGTAAACTC ATATCCGGTA CTGTCGATCA
     1201 AAGCGATCAG TCATTTTTAG ACGACGATCA AATTGAAGCT GGATACGTTC TCACCTGCGT
     1261 TGCTTACCCA ACTTCCGATT GCGTCATCGA AACACACAAA GAAGAAGAGC TTTACTAAga
     1321 attcTGATAA TAATCTAGAC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG
     1381 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA
     1441 CCTTCGGGTG GGCCTTTCTG CGTTTATAGG TACCCGTGGA TCCTCTAGAG TCGACCTGCA
     1501 GGCATGCAAG CTTTTTACGG CTAGCTCAGT CCTAGGTATA GTGCTAGCCC AGCCAGAGAA
     1561 ACACTCTTTA ACAGGGGGTA GTATGATGTC TCGTTTAGAT AAAAGTAAAG TGATTAACAG
     1621 CGCATTAGAG CTGCTTAATG AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA
     1681 GAAGCTAGGT GTAGAGCAGC CTACATTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT
     1741 CGACGCCTTA GCCATTGAGA TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAGAAGG
     1801 GGAAAGCTGG CAAGATTTTT TACGTAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG
     1861 TCATCGCGAT GGAGCAAAAG TACATTTAGG TACACGGCCT ACAGAAAAAC AGTATGAAAC
     1921 TCTCGAAAAT CAATTAGCCT TTTTATGCCA ACAAGGTTTT TCACTAGAGA ATGCATTATA
     1981 TGCACTCAGC GCAGTGGGGC ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA
     2041 AGTCGCTAAA GAAGAAAGGG AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA
     2101 AGCTATCGAA TTATTTGATC ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT
     2161 GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTTAAG GCATCAAATA
     2221 AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC
     2281 GCTCTCCTGA GTAGGACAAA TCCGCCGCCC TAGACCTAGG CGTTCGGCTG CGGCGAGCGG
     2341 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     2401 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     2461 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     2521 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     2581 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     2641 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     2701 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     2761 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     2821 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     2881 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     2941 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     3001 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     3061 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     3121 TGGTCATGAC TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
     3181 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
     3241 GAGCTCGATA TCAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT
     3301 AAGCATTCTG CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG
     3361 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA
     3421 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA
     3481 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA
     3541 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA
     3601 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC
     3661 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG
     3721 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT
     3781 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG
     3841 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT
     3901 GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC
     3961 GCCCGGTAGT GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC
     4021 GTCTCATTTT CGCCAGATAT CGACGT
//