LOCUS       pBW011                  4754 bp ds-DNA     circular     27-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS             1065..1094
                     /label="sRBS (2466.11 AU)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      3832..3936
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     RBS             3171..3204
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    2266..2937
                     /label="RFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3171..3171
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter region 1033..1038
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1125..1125
                     /label="(t) to remove secondary structure"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    977..982
                     /label="mutated BbsI site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3205..3831
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    complement(2036..2037)
                     /label="R1-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3159..3164
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2..107
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     Promoter region 1010..1015
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(124..783)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    1096..2940
                     /label="Fd ER 35 L10RFP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Promoter region 1016..1034
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter region 991..1009
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(4069..4751)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter        3136..3170
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1206..1207
                     /label="R1-L"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Promoter region 1045..1045
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        991..1064
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2230..2265
                     /label="L10"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1201..1205
                     /label="5 bp repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1206..2037
                     /label="Trans-ER LBD"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2962..3090
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3136..3141
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 GTGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC
       61 AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC
      121 AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC
      181 GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT
      241 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT
      301 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT
      361 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG
      421 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA
      481 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG
      541 CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT
      601 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT
      661 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA
      721 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC
      781 CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA
      841 TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG
      901 CCAGATATCG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
      961 ATCACGAGGC CCTTTCGTCT TGACCTCGAG TCCCTATCAG TGATAGAGAT TGACATCCCT
     1021 ATCAGTGATA GAGATACTGA GCACATCAGC AGGACGCACT GACCTAAAAA TCCGATCCCT
     1081 CTTTAGTTCT CAATCATGGC AACCTATAAA GTTACACTTA TTAACGAAGC TGAAGGTCTA
     1141 AACAAAACAA TTGAAGTTCC AGATGATCAG TACATTTTAG ATGCGGCTGA AGAAGCTGGT
     1201 ATAGATGCTG CAGCTGGGGG CGGAGGTTCT GGAGGTGGTG GGTCCAAGAA GAATTCACTG
     1261 GCGTTGTCGC TTACGGCTGA CCAAATGGTT TCTGCTCTGC TTGACGCCGA ACCTCCGATC
     1321 CTGTACTCTG AATACGACCC GACTCGTCCT TTTTCAGAGG CGAGTATGAT GGGGCTGCTT
     1381 ACGAATCTGG CAGACCGCGA GTTGGTACAT ATGATTAATT GGGCTAAACG TGTGCCGGGT
     1441 TTCGTGGACT TGACCCTGCA TGACCAGGTC CACCTGTTAG AATGCGCCTG GTTAGAGATT
     1501 TTAATGATCG GACTTGTCTG GCGTAGCATG GAACACCCCG GAAAACTGTT ATTTGCCCCT
     1561 AACTTGCTGC TGGACCGCAA CCAGGGGAAG TGTGTCGAAG GAATGGTGGA AATTTTCGAC
     1621 ATGCTTCTTG CCACTTCCTC GCGTTTTCGT ATGATGAATT TACAGGGAGA AGAGTTCGTT
     1681 TGTTTAAAGT CAATCATCTT ACTGAATAGC GGGGTTTACA CATTCTTATC TTCCACTCTT
     1741 AAATCACTGG AGGAGAAAGA CCATATTCAC CGTGTCCTGG ACAAGATCAC AGATACTCTT
     1801 ATCCATCTTA TGGCGAAGGC AGGCTTAACA CTTCAACAGC AACATCAGCG CCTTGCTCAG
     1861 CTTCTGTTGA TTTTAAGCCA TATCCGTCAC ATGTCAAACA AGGGTATGGA ACACCTTTAC
     1921 AGTATGAAGT GTAAGAACGT CGTTCCATTG TATGATTTGT TACTGGAAAT GCTTGACGCT
     1981 CACCGTCTGC ATGCACCTGG TGGCGGTGGC TCTGGTGGAG GCGGGAGCGC AGCTgCAATA
     2041 GACTTACCGT ATTCTTGTCG TGCTGGTGCT TGCTCTACCT GTGCTGGTAA ACTCATATCC
     2101 GGTACTGTCG ATCAAAGCGA TCAGTCATTT TTAGACGACG ATCAAATTGA AGCTGGATAC
     2161 GTTCTCACCT GCGTTGCTTA CCCAACTTCC GATTGCGTCA TCGAAACACA CAAAGAAGAA
     2221 GAGCTTTACG GGGGCTCTGG AGGTTCTGGA GGTTCTGGTG GGTCCGCGAG TAGCGAAGAC
     2281 GTTATCAAAG AGTTCATGCG TTTCAAAGTT CGTATGGAAG GTTCCGTTAA CGGTCACGAG
     2341 TTCGAAATCG AAGGTGAAGG TGAAGGTCGT CCGTACGAAG GTACCCAGAC CGCTAAACTG
     2401 AAAGTTACCA AAGGTGGTCC GCTGCCGTTC GCTTGGGACA TCCTGTCCCC GCAGTTCCAG
     2461 TACGGTTCCA AAGCTTACGT TAAACACCCG GCTGACATCC CGGACTACCT GAAACTGTCC
     2521 TTCCCGGAAG GTTTCAAATG GGAACGTGTT ATGAACTTCG AAGACGGTGG TGTTGTTACC
     2581 GTTACCCAGG ACTCCTCCCT GCAAGACGGT GAGTTCATCT ACAAAGTTAA ACTGCGTGGT
     2641 ACCAACTTCC CGTCCGACGG TCCGGTTATG CAGAAAAAAA CCATGGGTTG GGAAGCTTCC
     2701 ACCGAACGTA TGTACCCGGA AGACGGTGCT CTGAAAGGTG AAATCAAAAT GCGTCTGAAA
     2761 CTGAAAGACG GTGGTCACTA CGACGCTGAA GTTAAAACCA CCTACATGGC TAAAAAACCG
     2821 GTTCAGCTGC CGGGTGCTTA CAAAACCGAC ATCAAACTGG ACATCACCTC CCACAACGAA
     2881 GACTACACCA TCGTTGAACA GTACGAACGT GCTGAAGGTC GTCACTCCAC CGGTGCTTAA
     2941 gaattcTGAT AATAATCTAG ACCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT
     3001 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CTACTAGAGT CACACTGGCT
     3061 CACCTTCGGG TGGGCCTTTC TGCGTTTATA GGTACCCGTG GATCCTCTAG AGTCGACCTG
     3121 CAGGCATGCA AGCTTTTTAC GGCTAGCTCA GTCCTAGGTA TAGTGCTAGC CCAGCCAGAG
     3181 AAACACTCTT TAACAGGGGG TAGTATGATG TCTCGTTTAG ATAAAAGTAA AGTGATTAAC
     3241 AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT TAACAACCCG TAAACTCGCC
     3301 CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG TAAAAAATAA GCGGGCTTTG
     3361 CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA CTCACTTTTG CCCTTTAGAA
     3421 GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA GTTTTAGATG TGCTTTACTA
     3481 AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC CTACAGAAAA ACAGTATGAA
     3541 ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT TTTCACTAGA GAATGCATTA
     3601 TATGCACTCA GCGCAGTGGG GCATTTTACT TTAGGTTGCG TATTGGAAGA TCAAGAGCAT
     3661 CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA GTATGCCGCC ATTATTACGA
     3721 CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG CCTTCTTATT CGGCCTTGAA
     3781 TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA GTGGGTCTTA AGGCATCAAA
     3841 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
     3901 ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACCTA GGCGTTCGGC TGCGGCGAGC
     3961 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
     4021 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
     4081 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     4141 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     4201 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     4261 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     4321 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     4381 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     4441 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     4501 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     4561 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     4621 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     4681 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     4741 TTTGGTCATG ACTA
//