LOCUS       pBW012                  4046 bp ds-DNA     circular     27-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(2093..2094)
                     /label="R1-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        991..1064
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2254..2382
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2089..2092
                     /label="4bp gg overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(2..107)
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     CDS             complement(124..783)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    1258..1262
                     /label="5 bp duplicate"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2342..2382
                     /label="T7-TE terminator"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter region 1045..1045
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1096..2232
                     /label="Fd ER 54"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(3361..4043)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter region 1033..1038
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Promoter region 1016..1034
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1263..1264
                     /label="R1-L"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1265..1268
                     /label="4bp gg overhang"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2254..2333
                     /label="rrnBT1"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter region 991..1009
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             1065..1094
                     /label="sRBS (2466.11 AU)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    863..868
                     /label="-35"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1263..2094
                     /label="Trans-ER LBD"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Promoter        2428..2462
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2451..2456
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter region 1010..1015
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             2463..2496
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     CDS             2497..3123
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    2463..2463
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      3124..3228
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    2428..2433
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 GTGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC
       61 AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC
      121 AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC
      181 GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT
      241 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT
      301 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT
      361 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG
      421 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA
      481 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG
      541 CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT
      601 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT
      661 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA
      721 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC
      781 CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA
      841 TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG
      901 CCAGATATCG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
      961 ATCACGAGGC CCTTTCGTCT TCACCTCGAG TCCCTATCAG TGATAGAGAT TGACATCCCT
     1021 ATCAGTGATA GAGATACTGA GCACATCAGC AGGACGCACT GACCTAAAAA TCCGATCCCT
     1081 CTTTAGTTCT CAATCATGGC AACCTATAAA GTTACACTTA TTAACGAAGC TGAAGGTCTA
     1141 AACAAAACAA TTGAAGTTCC AGATGATCAG TACATTTTAG ATGCGGCTGA AGAAGCTGGT
     1201 ATAGACTTAC CGTATTCTTG TCGTGCTGGT GCTTGCTCTA CCTGTGCTGG TAAACTCATA
     1261 TCTGCTGCAG CTGGGGGCGG AGGTTCTGGA GGTGGTGGGT CCAAGAAGAA TTCACTGGCG
     1321 TTGTCGCTTA CGGCTGACCA AATGGTTTCT GCTCTGCTTG ACGCCGAACC TCCGATCCTG
     1381 TACTCTGAAT ACGACCCGAC TCGTCCTTTT TCAGAGGCGA GTATGATGGG GCTGCTTACG
     1441 AATCTGGCAG ACCGCGAGTT GGTACATATG ATTAATTGGG CTAAACGTGT GCCGGGTTTC
     1501 GTGGACTTGA CCCTGCATGA CCAGGTCCAC CTGTTAGAAT GCGCCTGGTT AGAGATTTTA
     1561 ATGATCGGAC TTGTCTGGCG TAGCATGGAA CACCCCGGAA AACTGTTATT TGCCCCTAAC
     1621 TTGCTGCTGG ACCGCAACCA GGGGAAGTGT GTCGAAGGAA TGGTGGAAAT TTTCGACATG
     1681 CTTCTTGCCA CTTCCTCGCG TTTTCGTATG ATGAATTTAC AGGGAGAAGA GTTCGTTTGT
     1741 TTAAAGTCAA TCATCTTACT GAATAGCGGG GTTTACACAT TCTTATCTTC CACTCTTAAA
     1801 TCACTGGAGG AGAAAGACCA TATTCACCGT GTCCTGGACA AGATCACAGA TACTCTTATC
     1861 CATCTTATGG CGAAGGCAGG CTTAACACTT CAACAGCAAC ATCAGCGCCT TGCTCAGCTT
     1921 CTGTTGATTT TAAGCCATAT CCGTCACATG TCAAACAAGG GTATGGAACA CCTTTACAGT
     1981 ATGAAGTGTA AGAACGTCGT TCCATTGTAT GATTTGTTAC TGGAAATGCT TGACGCTCAC
     2041 CGTCTGCATG CACCTGGTGG CGGTGGCTCT GGTGGAGGCG GGAGCGCAGC TgCAATATCC
     2101 GGTACTGTCG ATCAAAGCGA TCAGTCATTT TTAGACGACG ATCAAATTGA AGCTGGATAC
     2161 GTTCTCACCT GCGTTGCTTA CCCAACTTCC GATTGCGTCA TCGAAACACA CAAAGAAGAA
     2221 GAGCTTTACT AAgaattcTG ATAATAATCT AGACCAGGCA TCAAATAAAA CGAAAGGCTC
     2281 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCTACTAGA
     2341 GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAGGTACCCG TGGATCCTCT
     2401 AGAGTCGACC TGCAGGCATG CAAGCTTTTT ACGGCTAGCT CAGTCCTAGG TATAGTGCTA
     2461 GCCCAGCCAG AGAAACACTC TTTAACAGGG GGTAGTATGA TGTCTCGTTT AGATAAAAGT
     2521 AAAGTGATTA ACAGCGCATT AGAGCTGCTT AATGAGGTCG GAATCGAAGG TTTAACAACC
     2581 CGTAAACTCG CCCAGAAGCT AGGTGTAGAG CAGCCTACAT TGTATTGGCA TGTAAAAAAT
     2641 AAGCGGGCTT TGCTCGACGC CTTAGCCATT GAGATGTTAG ATAGGCACCA TACTCACTTT
     2701 TGCCCTTTAG AAGGGGAAAG CTGGCAAGAT TTTTTACGTA ATAACGCTAA AAGTTTTAGA
     2761 TGTGCTTTAC TAAGTCATCG CGATGGAGCA AAAGTACATT TAGGTACACG GCCTACAGAA
     2821 AAACAGTATG AAACTCTCGA AAATCAATTA GCCTTTTTAT GCCAACAAGG TTTTTCACTA
     2881 GAGAATGCAT TATATGCACT CAGCGCAGTG GGGCATTTTA CTTTAGGTTG CGTATTGGAA
     2941 GATCAAGAGC ATCAAGTCGC TAAAGAAGAA AGGGAAACAC CTACTACTGA TAGTATGCCG
     3001 CCATTATTAC GACAAGCTAT CGAATTATTT GATCACCAAG GTGCAGAGCC AGCCTTCTTA
     3061 TTCGGCCTTG AATTGATCAT ATGCGGATTA GAAAAACAAC TTAAATGTGA AAGTGGGTCT
     3121 TAAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT
     3181 GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GCCCTAGACC TAGGCGTTCG
     3241 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
     3301 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
     3361 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     3421 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     3481 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     3541 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC
     3601 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     3661 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     3721 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     3781 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
     3841 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     3901 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     3961 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     4021 ACGTTAAGGG ATTTTGGTCA TGACTA
//