LOCUS pMH48 6765 bp DNA circular SYN 13-AUG-2019 DEFINITION ACCESSION pMH48 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 6765) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software FEATURES Location/Qualifiers misc_feature 5..25 /gene="lac operator" misc_feature 58..69 /gene="RBS" CDS 70..1611 /gene="Cph1 (1-514)" CDS 70..1671 /gene="Cph1*" /translation="MATTVQLSDQSLRQLETLAIHTAHLIQPHGLVVVLQEPDLTISQ ISANCTGILGRSPEDLLGRTLGEVFDSFQIDPIQSRLTAGQISSLNPSKLWARVMGDD FVIFDGVFHRNSDGLLVCELEPAYTSDNLPFLGFYHMANAALNRLRQQANLRDFYDVI VEEVRRMTGFDRVMLYRFDENNHGDVIAEDKRDDMEPYLGLHYPESDIPQPARRLFIH NPIRVIPDVYGVAVPLTPAVNPSTNRAVDLTESILRSAYHCHLTFLKNMGVGASLTIS LIKDGHLWGLIACHHQTPKVIPFELRKACEFFGRVVFSNISAQEDTETFDYRVQLAEH EAVLLDKMTTAADFVEGLTNHPDRLLGLTGSQGAAICFGEKLILVGETPDEKAVQYLL QWLENREVQDVFFTSSLSQIYPDAVNFKSVASGLLAIPIARHNFLLWFRPEVLQTVNW GGDPNHAYEATQEDGKIELHPRQSFDLWKEIVRLQSLPWQSVEIQSALALKKAIVNLI LRQAEEGRGDSPGRGDSPHHHHHHC" CDS 856..858 /gene="Y263F" CDS 1612..1647 /gene="RGD motif" CDS 1648..1665 /gene="His6" CDS 1666..1668 /gene="Cys" misc_feature 1737..1753 /gene="T7 promoter-2" misc_feature 1758..1778 /gene="lac operator" CDS 1823..2545 /gene="HO1" /translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVG NLYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAG QAYVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFAD IDDEKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMV FNSLTRRRSQGSTEVGLATSEG" CDS 2580..3326 /gene="PcyA" /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPE DLGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLF GCDIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSE YCLFIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQK NDKTRRVLEKAFGEAWAERYMSQVLFDVIQ" misc_feature 3446..3493 /gene="T7 terminator" CDS complement(3664..4455) /gene="aadA (SmR)" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" misc_feature 4595..5333 /gene="CDF ori" CDS complement(5543..6625) /gene="lacI" /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" misc_feature 6749..6765 /gene="T7 promoter-1" ORIGIN 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaatcag 61 aaggaataca tggccaccac cgtacaactc agcgaccaat ccctccgtca gctagaaacc 121 ctcgccatcc acaccgccca cctgattcag ccccacggtt tagtggtggt cctgcaggaa 181 ccagacctca ccatcagcca aattagcgcc aactgcaccg gcattttagg gcgatcgcca 241 gaggatttgt tgggcagaac cctaggggaa gtgtttgata gctttcagat tgatcccatc 301 cagagtcgcc taacggccgg acaaatcagc agcctcaacc ccagtaaact ttgggcgcgg 361 gtcatggggg acgactttgt catttttgac ggggtttttc atcgcaacag tgacggttta 421 ttggtatgtg aactagagcc agcctacact tccgataatc tgcccttcct cggtttttat 481 cacatggcca acgctgccct gaatcggttg cgccaacaag ctaatctacg ggatttctac 541 gatgttattg tcgaagaagt ccgccgtatg actggctttg accgggtgat gctataccgc 601 tttgatgaaa ataaccacgg tgatgtcatt gccgaagata aacgggatga tatggaaccc 661 tatttgggcc tgcactatcc cgaatcggat attccccaac ccgcccgtcg gctatttatc 721 cacaacccca ttcgagtaat tcccgatgtt tatggtgtgg cggtgcccct gaccccagcg 781 gttaacccca gcaccaaccg agcggtggat ttaacagaat ccattctgcg cagtgcgtac 841 cattgccact tgacctttct gaaaaatatg ggggtaggag cgtctttaac catttcccta 901 attaaggacg gccatctctg ggggctcatt gcctgccacc atcaaacccc caaagtaatt 961 ccctttgaac tgcgtaaagc ctgcgaattt tttggtcggg tggtgtttag caacatttcc 1021 gcccaggaag atacggaaac cttcgattac cgggtgcagt tggcggagca tgaagcggtt 1081 ttattggaca aaatgaccac ggcggcggat tttgtcgaag gattaactaa tcatcccgat 1141 cgcctgttgg gattaacggg ctcccagggg gcggccattt gctttgggga aaaattgatt 1201 ttagtagggg aaaccccgga cgagaaagca gtgcaatatt tactgcaatg gttggagaat 1261 cgggaagtgc aagacgtttt cttcacctct tccctctcac aaatttatcc tgatgcagtg 1321 aattttaaat ccgtggccag tggcttattg gccattccca ttgcccgtca caactttttg 1381 ctctggtttc gccctgaagt gttgcaaacg gttaattggg gcggtgaccc aaatcatgct 1441 tacgaagcta cccaggaaga cggtaaaatc gagctccatc cccgccaatc ctttgacctc 1501 tggaaagaaa ttgtccgact ccaatctttg ccctggcaat cggtggaaat ccaaagtgcc 1561 ctggccctga aaaaggcgat cgtcaacctc attttgcgcc aggcagaaga aggccgtggc 1621 gatagcccag gtcgtggtga ctctccgcat catcaccatc accattgctg agcggccgca 1681 taatgcttaa gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat 1741 acgactcact ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta 1801 agtataagaa ggagatatac atatgagtgt caacttagct tcccagttgc gggaagggac 1861 gaaaaaatcc cactccatgg cggagaacgt cggctttgtc aaatgcttcc tcaagggcgt 1921 tgtcgagaaa aattcctacc gtaagctggt tggcaatctc tactttgtct acagtgccat 1981 ggaagaggaa atggcaaaat ttaaggacca tcccatcctc agccacattt acttccccga 2041 actcaaccgc aaacaaagcc tagagcaaga cctgcaattc tattacggct ccaactggcg 2101 gcaagaagtg aaaatttctg ccgctggcca agcctatgtg gaccgagtcc ggcaagtggc 2161 cgctacggcc cctgaattgt tggtggccca ttcctacacc cgttacctgg gggatctttc 2221 cggcggtcaa attctcaaga aaattgccca aaatgccatg aatctccacg atggtggcac 2281 agctttctat gaatttgccg acattgatga cgaaaaggct tttaaaaata cctaccgtca 2341 agctatgaat gatctgccca ttgaccaagc caccgccgaa cggattgtgg atgaagccaa 2401 tgacgccttt gccatgaaca tgaaaatgtt caacgaactt gaaggcaacc tgatcaaggc 2461 gatcggcatt atggtgttca acagcctcac ccgtcgccgc agtcaaggca gcaccgaagt 2521 tggcctcgcc acctccgaag gctagaagct tggatccatt aaagaggaga aacccgggca 2581 tggccgtcac tgatttaagt ttgaccaatt cttccctgat gcctacgttg aacccgatga 2641 ttcaacagtt ggccctggcg atcgccgcta gttggcaaag tttacccctc aagccctatc 2701 aattgccgga ggatttgggc tacgtagaag gccgcctgga aggggaaaag ttagtgattg 2761 aaaatcggtg ctaccaaacg ccccagtttc gcaaaatgca tttggagttg gccaaggtgg 2821 gcaaagggtt ggatattctc cactgtgtaa tgtttcctga gcctttatac ggtctacctt 2881 tgtttggctg tgacattgtg gccggccccg gtggagtaag tgcggctatt gcggatctat 2941 cccccaccca aagcgatcgc caattgcccg cagcgtacca aaaatcattg gcagagctag 3001 gccagccaga atttgagcaa caacgggaat tgcccccctg gggagaaata ttttctgaat 3061 attgtttatt catccgtccc agcaatgtca ctgaagaaga aagatttgta caaagggtag 3121 tggacttttt gcaaattcat tgtcaccaat ccatcgttgc cgaacccttg tctgaagctc 3181 aaactttgga gcaccgtcag gggcaaattc attactgcca acaacaacag aaaaatgata 3241 aaacccgtcg ggtactggaa aaagcttttg gggaagcttg ggcggaacgg tatatgagcc 3301 aagtcttatt tgatgttatc caataagacg tcggtaccct cgagtctggt aaagaaaccg 3361 ctgctgcgaa attgaacgcc agcacatgga ctcgtctact agcgcagctt aattaaccta 3421 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 3481 gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt 3541 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga 3601 accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag 3661 acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa 3721 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc 3781 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc 3841 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac 3901 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag 3961 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac 4021 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt 4081 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc 4141 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac 4201 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa 4261 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact 4321 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg 4381 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat 4441 caccgcttcc ctcatactct tcctttttca atattattga agcatttatc agggttattg 4501 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact 4561 cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc 4621 cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg 4681 gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc 4741 ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga 4801 caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc 4861 gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt 4921 tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct 4981 gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg 5041 agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt 5101 tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg 5161 ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag 5221 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta 5281 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5341 ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc 5401 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct 5461 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta 5521 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 5581 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg 5641 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct 5701 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga 5761 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg 5821 agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca 5881 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg 5941 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt 6001 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta 6061 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca 6121 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat 6181 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat 6241 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg 6301 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt 6361 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg 6421 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg 6481 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt 6541 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga 6601 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct 6661 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct 6721 cccttatgcg actcctgcat taggaaatta atacgactca ctata //