LOCUS       pMH610                  7198 bp    DNA     circular SYN 13-AUG-2019
DEFINITION  
ACCESSION   pMH610
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    5..25
                     /gene="lac operator"
     CDS             71..2023
                     /gene="PhyB codon optimized (1-651)"
     CDS             71..2104
                     /gene="PhyB*"
                     /translation="MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKS
                     LRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQIT
                     AYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMG
                     TDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEP
                     ARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVY
                     KFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLV
                     VQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRS
                     SMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTL
                     LCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLA
                     NHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGA
                     KHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAA
                     MNSKVVDGVVQPCRDMAGEQGIDELGAGSGSGRGDSPGRGDSPHHHHHHDRCG"
     CDS             2036..2071
                     /gene="RGD motif"
     CDS             2072..2089
                     /gene="His6"
     CDS             2096..2098
                     /gene="Cys"
     misc_feature    2170..2186
                     /gene="T7 promoter-2"
     misc_feature    2191..2211
                     /gene="lac operator"
     CDS             2256..2978
                     /gene="HO1"
                     /translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVG
                     NLYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAG
                     QAYVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFAD
                     IDDEKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMV
                     FNSLTRRRSQGSTEVGLATSEG"
     CDS             3013..3759
                     /gene="PcyA"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPE
                     DLGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLF
                     GCDIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSE
                     YCLFIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQK
                     NDKTRRVLEKAFGEAWAERYMSQVLFDVIQ"
     misc_feature    3879..3926
                     /gene="T7 terminator"
     CDS             complement(4097..4888)
                     /gene="aadA (SmR)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5028..5766
                     /gene="CDF ori"
     CDS             complement(5976..7058)
                     /gene="lacI"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    7182..7198
                     /gene="T7 promoter-1"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggttagcg gtgttggtgg tagcggtggt ggtcgtggtg gcggtcgcgg
      121 aggtgaagaa gaaccgagca gcagccatac cccgaataat cgccgtggtg gtgaacaggc
      181 acagagcagc ggcaccaaaa gcctgcgtcc gcgtagcaat accgaaagca tgagcaaagc
      241 aattcagcag tataccgttg atgcacgtct gcatgccgtt ttcgaacaga gcggtgaaag
      301 cggtaaaagc tttgattata gccagagcct gaaaaccacc acctatggta gcagcgtgcc
      361 ggaacagcag attaccgcat atctgagccg tattcagcgt ggtggctaca ttcagccgtt
      421 tggctgcatg atcgcagttg atgaaagcag ctttcgcatt attggctaca gcgaaaatgc
      481 acgtgaaatg ctgggcatta tgccgcagag cgttccgacc ctggaaaaac cggaaattct
      541 ggcaatgggc accgatgttc gtagcctgtt taccagcagc agctccattc tgctggaacg
      601 tgcctttgtt gcccgtgaaa ttaccctgct gaatccggtt tggattcata gcaaaaacac
      661 cggcaaaccg ttttatgcaa ttctgcatcg tattgatgtt ggcgtggtta ttgatctgga
      721 accggcacgt accgaagatc cggcactgag cattgccggt gcagttcaga gccagaaact
      781 ggcagttcgt gcaattagcc agctgcaggc actgcctggt ggtgatatca aactgctgtg
      841 tgataccgtt gttgaaagcg ttcgtgatct gaccggctac gaccgtgtta tggtgtataa
      901 attccacgaa gatgaacatg gtgaagttgt tgcagaaagc aaacgtgatg acctggaacc
      961 gtatattggt ctgcattatc cagcaaccga tattccgcag gcaagccgtt tcctgttcaa
     1021 acagaatcgt gtgcgcatga ttgttgattg taatgcaaca ccggttctgg ttgttcagga
     1081 tgatcgtctg acccagagca tgtgtctggt tggtagcacc ctgcgtgcac cgcatggttg
     1141 tcatagccag tatatggcaa atatgggtag catcgcaagc ctggccatgg cggtgatcat
     1201 caatggtaat gaagatgatg gtagcaatgt tgcaagcggt cgtagcagca tgcgtctgtg
     1261 gggtctggtt gtgtgtcatc ataccagcag tcgctgcatt ccgtttccgc tgcgttatgc
     1321 atgtgaattt ctgatgcagg catttggact gcagctgaat atggaactgc aactggcact
     1381 gcagatgagc gaaaaacgtg ttctgcgtac ccagaccctg ctgtgcgata tgctgctgcg
     1441 tgatagtccg gcaggcattg ttacccagag cccgagcatt atggatctgg tgaaatgcga
     1501 tggtgcagcc tttctgtatc acggtaaata ctatccgctg ggtgttgcac cgagcgaagt
     1561 tcagattaaa gatgttgttg agtggctgct ggcaaatcat gcagatagca ccggtctgag
     1621 caccgatagc ctgggtgatg caggttatcc gggtgcagca gcactgggag atgcagtttg
     1681 tggtatggca gttgcataca ttaccaaacg cgattttctg ttttggtttc gtagccatac
     1741 cgccaaagaa atcaaatggg gtggtgcaaa acatcacccg gaagataaag atgacggtca
     1801 gcgtatgcat ccgcgtagta gctttcaggc atttctggaa gtggtgaaaa gccgtagcca
     1861 gccgtgggaa accgcagaaa tggatgcaat tcatagcctg caactgattc tgcgcgatag
     1921 cttcaaagaa agcgaagcag caatgaatag caaagttgtt gatggtgttg ttcagccgtg
     1981 tcgtgatatg gcaggcgaac agggtattga tgaactgggt gcaggttctg gtagcggtcg
     2041 tggcgatagc ccaggtcgtg gtgactctcc gcatcatcac catcaccatg atcgctgtgg
     2101 ttaagcggcc gcataatgct taagtcgaac agaaagtaat cgtattgtac acggccgcat
     2161 aatcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccatctt
     2221 agtatattag ttaagtataa gaaggagata tacatatgag tgtcaactta gcttcccagt
     2281 tgcgggaagg gacgaaaaaa tcccactcca tggcggagaa cgtcggcttt gtcaaatgct
     2341 tcctcaaggg cgttgtcgag aaaaattcct accgtaagct ggttggcaat ctctactttg
     2401 tctacagtgc catggaagag gaaatggcaa aatttaagga ccatcccatc ctcagccaca
     2461 tttacttccc cgaactcaac cgcaaacaaa gcctagagca agacctgcaa ttctattacg
     2521 gctccaactg gcggcaagaa gtgaaaattt ctgccgctgg ccaagcctat gtggaccgag
     2581 tccggcaagt ggccgctacg gcccctgaat tgttggtggc ccattcctac acccgttacc
     2641 tgggggatct ttccggcggt caaattctca agaaaattgc ccaaaatgcc atgaatctcc
     2701 acgatggtgg cacagctttc tatgaatttg ccgacattga tgacgaaaag gcttttaaaa
     2761 atacctaccg tcaagctatg aatgatctgc ccattgacca agccaccgcc gaacggattg
     2821 tggatgaagc caatgacgcc tttgccatga acatgaaaat gttcaacgaa cttgaaggca
     2881 acctgatcaa ggcgatcggc attatggtgt tcaacagcct cacccgtcgc cgcagtcaag
     2941 gcagcaccga agttggcctc gccacctccg aaggctagaa gcttggatcc attaaagagg
     3001 agaaacccgg gcatggccgt cactgattta agtttgacca attcttccct gatgcctacg
     3061 ttgaacccga tgattcaaca gttggccctg gcgatcgccg ctagttggca aagtttaccc
     3121 ctcaagccct atcaattgcc ggaggatttg ggctacgtag aaggccgcct ggaaggggaa
     3181 aagttagtga ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat gcatttggag
     3241 ttggccaagg tgggcaaagg gttggatatt ctccactgtg taatgtttcc tgagccttta
     3301 tacggtctac ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt aagtgcggct
     3361 attgcggatc tatcccccac ccaaagcgat cgccaattgc ccgcagcgta ccaaaaatca
     3421 ttggcagagc taggccagcc agaatttgag caacaacggg aattgccccc ctggggagaa
     3481 atattttctg aatattgttt attcatccgt cccagcaatg tcactgaaga agaaagattt
     3541 gtacaaaggg tagtggactt tttgcaaatt cattgtcacc aatccatcgt tgccgaaccc
     3601 ttgtctgaag ctcaaacttt ggagcaccgt caggggcaaa ttcattactg ccaacaacaa
     3661 cagaaaaatg ataaaacccg tcgggtactg gaaaaagctt ttggggaagc ttgggcggaa
     3721 cggtatatga gccaagtctt atttgatgtt atccaataag acgtcggtac cctcgagtct
     3781 ggtaaagaaa ccgctgctgc gaaattgaac gccagcacat ggactcgtct actagcgcag
     3841 cttaattaac ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     3901 ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca
     3961 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     4021 gaccctgccc tgaaccgacg accgggtcat cgtggccgga tcttgcggcc cctcggcttg
     4081 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     4141 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     4201 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     4261 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
     4321 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
     4381 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
     4441 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
     4501 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
     4561 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
     4621 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
     4681 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
     4741 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
     4801 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
     4861 atacttcggc gatcaccgct tccctcatac tcttcctttt tcaatattat tgaagcattt
     4921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4981 tagctagctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg ctgcggacac
     5041 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacag
     5101 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
     5161 cagacgcttt tccggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
     5221 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggcgggtcgc tccctcttgc
     5281 gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgcctttct cccttacggg
     5341 aagtgtggcg ctttctcata gctcacacac tggtatctcg gctcggtgta ggtcgttcgc
     5401 tccaagctgg gctgtaagca agaactcccc gttcagcccg actgctgcgc cttatccggt
     5461 aactgttcac ttgagtccaa cccggaaaag cacggtaaaa cgccactggc agcagccatt
     5521 ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg
     5581 cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt
     5641 taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg
     5701 gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct
     5761 ttgatctttt ctactgaacc gctctagatt tcagtgcaat ttatctcttc aaatgtagca
     5821 cctgaagtca gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc
     5881 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     5941 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6001 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6061 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     6121 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6181 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     6241 tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     6301 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     6361 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     6421 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     6481 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     6541 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     6601 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     6661 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     6721 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     6781 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     6841 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     6901 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     6961 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     7021 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     7081 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     7141 atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata
//
