LOCUS       pMH1105                 7195 bp    DNA     circular SYN 13-AUG-2019
DEFINITION  
ACCESSION   pMH1105
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    5..25
                     /gene="lac operator"
     CDS             71..2023
                     /gene="PhyB codon optimized (1-651)"
     CDS             71..2101
                     /gene="PhyB-AviTag"
                     /translation="MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKS
                     LRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQIT
                     AYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMG
                     TDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEP
                     ARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVY
                     KFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLV
                     VQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRS
                     SMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTL
                     LCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLA
                     NHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGA
                     KHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAA
                     MNSKVVDGVVQPCRDMAGEQGIDELGAGSGSGLNDIFEAQKIEWHEHHHHHH"
     CDS             2036..2080
                     /gene="AviTag"
     CDS             2081..2098
                     /gene="His6"
     misc_feature    2167..2183
                     /gene="T7 promoter-2"
     misc_feature    2188..2208
                     /gene="lac operator"
     CDS             2253..2975
                     /gene="HO1"
                     /translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVG
                     NLYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAG
                     QAYVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFAD
                     IDDEKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMV
                     FNSLTRRRSQGSTEVGLATSEG"
     CDS             3010..3756
                     /gene="PcyA"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPE
                     DLGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLF
                     GCDIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSE
                     YCLFIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQK
                     NDKTRRVLEKAFGEAWAERYMSQVLFDVIQ"
     misc_feature    3876..3923
                     /gene="T7 terminator"
     CDS             complement(4094..4885)
                     /gene="aadA (SmR)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5025..5763
                     /gene="CDF ori"
     CDS             complement(5973..7055)
                     /gene="lacI"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    7179..7195
                     /gene="T7 promoter-1"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggttagcg gtgttggtgg tagcggtggt ggtcgtggtg gcggtcgcgg
      121 aggtgaagaa gaaccgagca gcagccatac cccgaataat cgccgtggtg gtgaacaggc
      181 acagagcagc ggcaccaaaa gcctgcgtcc gcgtagcaat accgaaagca tgagcaaagc
      241 aattcagcag tataccgttg atgcacgtct gcatgccgtt ttcgaacaga gcggtgaaag
      301 cggtaaaagc tttgattata gccagagcct gaaaaccacc acctatggta gcagcgtgcc
      361 ggaacagcag attaccgcat atctgagccg tattcagcgt ggtggctaca ttcagccgtt
      421 tggctgcatg atcgcagttg atgaaagcag ctttcgcatt attggctaca gcgaaaatgc
      481 acgtgaaatg ctgggcatta tgccgcagag cgttccgacc ctggaaaaac cggaaattct
      541 ggcaatgggc accgatgttc gtagcctgtt taccagcagc agctccattc tgctggaacg
      601 tgcctttgtt gcccgtgaaa ttaccctgct gaatccggtt tggattcata gcaaaaacac
      661 cggcaaaccg ttttatgcaa ttctgcatcg tattgatgtt ggcgtggtta ttgatctgga
      721 accggcacgt accgaagatc cggcactgag cattgccggt gcagttcaga gccagaaact
      781 ggcagttcgt gcaattagcc agctgcaggc actgcctggt ggtgatatca aactgctgtg
      841 tgataccgtt gttgaaagcg ttcgtgatct gaccggctac gaccgtgtta tggtgtataa
      901 attccacgaa gatgaacatg gtgaagttgt tgcagaaagc aaacgtgatg acctggaacc
      961 gtatattggt ctgcattatc cagcaaccga tattccgcag gcaagccgtt tcctgttcaa
     1021 acagaatcgt gtgcgcatga ttgttgattg taatgcaaca ccggttctgg ttgttcagga
     1081 tgatcgtctg acccagagca tgtgtctggt tggtagcacc ctgcgtgcac cgcatggttg
     1141 tcatagccag tatatggcaa atatgggtag catcgcaagc ctggccatgg cggtgatcat
     1201 caatggtaat gaagatgatg gtagcaatgt tgcaagcggt cgtagcagca tgcgtctgtg
     1261 gggtctggtt gtgtgtcatc ataccagcag tcgctgcatt ccgtttccgc tgcgttatgc
     1321 atgtgaattt ctgatgcagg catttggact gcagctgaat atggaactgc aactggcact
     1381 gcagatgagc gaaaaacgtg ttctgcgtac ccagaccctg ctgtgcgata tgctgctgcg
     1441 tgatagtccg gcaggcattg ttacccagag cccgagcatt atggatctgg tgaaatgcga
     1501 tggtgcagcc tttctgtatc acggtaaata ctatccgctg ggtgttgcac cgagcgaagt
     1561 tcagattaaa gatgttgttg agtggctgct ggcaaatcat gcagatagca ccggtctgag
     1621 caccgatagc ctgggtgatg caggttatcc gggtgcagca gcactgggag atgcagtttg
     1681 tggtatggca gttgcataca ttaccaaacg cgattttctg ttttggtttc gtagccatac
     1741 cgccaaagaa atcaaatggg gtggtgcaaa acatcacccg gaagataaag atgacggtca
     1801 gcgtatgcat ccgcgtagta gctttcaggc atttctggaa gtggtgaaaa gccgtagcca
     1861 gccgtgggaa accgcagaaa tggatgcaat tcatagcctg caactgattc tgcgcgatag
     1921 cttcaaagaa agcgaagcag caatgaatag caaagttgtt gatggtgttg ttcagccgtg
     1981 tcgtgatatg gcaggcgaac agggtattga tgaactgggt gcaggttctg gtagcggtct
     2041 gaacgacatc ttcgaagctc agaaaatcga atggcacgaa catcatcacc atcaccatta
     2101 agcggccgca taatgcttaa gtcgaacaga aagtaatcgt attgtacacg gccgcataat
     2161 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc ccatcttagt
     2221 atattagtta agtataagaa ggagatatac atatgagtgt caacttagct tcccagttgc
     2281 gggaagggac gaaaaaatcc cactccatgg cggagaacgt cggctttgtc aaatgcttcc
     2341 tcaagggcgt tgtcgagaaa aattcctacc gtaagctggt tggcaatctc tactttgtct
     2401 acagtgccat ggaagaggaa atggcaaaat ttaaggacca tcccatcctc agccacattt
     2461 acttccccga actcaaccgc aaacaaagcc tagagcaaga cctgcaattc tattacggct
     2521 ccaactggcg gcaagaagtg aaaatttctg ccgctggcca agcctatgtg gaccgagtcc
     2581 ggcaagtggc cgctacggcc cctgaattgt tggtggccca ttcctacacc cgttacctgg
     2641 gggatctttc cggcggtcaa attctcaaga aaattgccca aaatgccatg aatctccacg
     2701 atggtggcac agctttctat gaatttgccg acattgatga cgaaaaggct tttaaaaata
     2761 cctaccgtca agctatgaat gatctgccca ttgaccaagc caccgccgaa cggattgtgg
     2821 atgaagccaa tgacgccttt gccatgaaca tgaaaatgtt caacgaactt gaaggcaacc
     2881 tgatcaaggc gatcggcatt atggtgttca acagcctcac ccgtcgccgc agtcaaggca
     2941 gcaccgaagt tggcctcgcc acctccgaag gctagaagct tggatccatt aaagaggaga
     3001 aacccgggca tggccgtcac tgatttaagt ttgaccaatt cttccctgat gcctacgttg
     3061 aacccgatga ttcaacagtt ggccctggcg atcgccgcta gttggcaaag tttacccctc
     3121 aagccctatc aattgccgga ggatttgggc tacgtagaag gccgcctgga aggggaaaag
     3181 ttagtgattg aaaatcggtg ctaccaaacg ccccagtttc gcaaaatgca tttggagttg
     3241 gccaaggtgg gcaaagggtt ggatattctc cactgtgtaa tgtttcctga gcctttatac
     3301 ggtctacctt tgtttggctg tgacattgtg gccggccccg gtggagtaag tgcggctatt
     3361 gcggatctat cccccaccca aagcgatcgc caattgcccg cagcgtacca aaaatcattg
     3421 gcagagctag gccagccaga atttgagcaa caacgggaat tgcccccctg gggagaaata
     3481 ttttctgaat attgtttatt catccgtccc agcaatgtca ctgaagaaga aagatttgta
     3541 caaagggtag tggacttttt gcaaattcat tgtcaccaat ccatcgttgc cgaacccttg
     3601 tctgaagctc aaactttgga gcaccgtcag gggcaaattc attactgcca acaacaacag
     3661 aaaaatgata aaacccgtcg ggtactggaa aaagcttttg gggaagcttg ggcggaacgg
     3721 tatatgagcc aagtcttatt tgatgttatc caataagacg tcggtaccct cgagtctggt
     3781 aaagaaaccg ctgctgcgaa attgaacgcc agcacatgga ctcgtctact agcgcagctt
     3841 aattaaccta ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     3901 aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac
     3961 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
     4021 cctgccctga accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac
     4081 gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     4141 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     4201 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     4261 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     4321 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     4381 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     4441 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     4501 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     4561 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
     4621 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     4681 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     4741 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     4801 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     4861 cttcggcgat caccgcttcc ctcatactct tcctttttca atattattga agcatttatc
     4921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4981 ctagctcact cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata
     5041 caaagttacc cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag
     5101 ggccgcgccg gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag
     5161 acgcttttcc ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg
     5221 cgaaacccga caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct
     5281 ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag
     5341 tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc
     5401 aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac
     5461 tgttcacttg agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt
     5521 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
     5581 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
     5641 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
     5701 ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5761 atcttttcta ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     5821 gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc
     5881 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     5941 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6001 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6061 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     6121 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     6181 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     6241 cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     6301 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     6361 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     6421 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     6481 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     6541 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     6601 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     6661 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     6721 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     6781 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     6841 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     6901 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     6961 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     7021 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     7081 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
     7141 tcgacgctct cccttatgcg actcctgcat taggaaatta atacgactca ctata
//
