LOCUS pAAV-EF1a-DIO-ASAP3Kv-W 7111 bp ds-DNA circular 13-AUG-2019 DEFINITION . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers repeat_region 4374..4503 /label="AAV2 ITR" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 repeat_region 1..130 /label="AAV2 ITR (alternate)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter 5326..5430 /label="AmpR promoter" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer_bind 5180..5202 /label="pGEX 3'" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS complement(3709..3720) /label="Factor Xa site" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind 4886..4907 /label="F1ori-F" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin 4589..5044 /label="f1 ori" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 intron 462..1400 /label="EF-1-alpha intron A" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature complement(1572..1766) /label="KV2.1" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 protein_bind complement(3096..3129) /label="lox2272" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS 5431..6291 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 3090..3095 /label="NEHI" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 rep_origin 6462..7050 /label="ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter 231..1409 /label="EF-1-alpha promoter" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 source 1..7111 /label="source" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind complement(3291..3311) /label="WPRE-R" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e protein_bind 1524..1557 /label="loxP" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd polyA_signal 3858..4334 /label="hGH poly(A) signal" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind complement(4676..4695) /label="F1ori-R" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e protein_bind complement(3180..3213) /label="loxP" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 protein_bind 1440..1473 /label="lox2272" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(4057..4076) /label="hGH-PA-R" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature complement(1773..1799) /label="GSS" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 1558..1565 /label="ASCI" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 6951..6970 /label="pBR322ori-F" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(5649..5668) /label="Amp-R" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 1357..1377 /label="EF1a-F" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 repeat_region 4374..4514 /label="AAV2 ITR" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(5061..5080) /label="pRS-marker" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(5240..5258) /label="pBRforEco" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 3238..3826 /label="WPRE" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature complement(1806..3083) /label="ASAP3" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga 181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1381 gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc gactccggaa 1441 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc 1501 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc 1561 gcgcctcatt aaaagcgggt cgcttccgga aaatcggtcg cgcagctaat aaagctatca 1621 atgctgctca tgctgcgcat atcaatcacg ccttcggtgc gcgccggcag cggcgccacc 1681 gggctcggca tgctgctcat ttccagttct tccggcggtt tgctctgcgg cgccatttct 1741 ttggtgttca gaatcggctg gctctgacgc gtggaagatc cagatgaacc tgaagaaccc 1801 ttaagggtta ccacttcaag ttgtttcttc tgtgaagcca gctgaaatat tcttattaag 1861 ataacaattc tcagaactcg aagaagagta accatctgcg gcatctgatc tgtgttatat 1921 tccagtttat gccccagaat gttgccatct tctttaaaat cggtgccttt cagttcaatg 1981 cggttcacca gggtatcgcc ttcaaatttc accaccgcgc gggttttata tttgccatca 2041 tctttaaagc taatggtgcg ttcctgcaca tagccttccg gcatcgcgct tttaaaaaaa 2101 tcatggcgtt tcatatgatc cggatagcgg ctaaagcact gcacgccata ggttaaggtg 2161 gtcaccaggg tcggccacgg caccggtagt ttgccggtgg tgcaaataaa tttcagggtc 2221 agtttgccaa tggtcgcatc gccttcgcct tcgccgcgca cgctaaattt atggccgttc 2281 acatcgccat ccagttccac cagaatcggc accacgccgg taaacagttc ttcgccctgg 2341 ctcgcgctgc cgccggtgcc gccatacagt tcatccatgc cgtgtgtaat gcccgctgcg 2401 gtcacaaatt ccagcagcac catgtgatcg cgtttttcgt tcggatcttt gctcagcacg 2461 gtctgggtgc tcagataatg gttatccggc agcagcaccg ggccatcgcc aatcggggtg 2521 ttctgctgat aatgatccgc cagctgcacg ctgccatctt ccacgttatg gcgcacggta 2581 aagttcgctt taatgccgtt tttctgttta tccgcggtaa tatacacgtt atcaccgcga 2641 aatgtaccgt cagagaagga gtaggtcata ttaattagca gagtgcccac tactatgact 2701 gcatccaaag tattcagttt ggaccggaaa tagttcttga agccttcaac aaacactctc 2761 atgagaacat caacaaggaa gaagagtgct atagccaggg aaacaccttc aagaatctct 2821 ctaatgcctc ttttcttctc actgatggcc agatccacaa tcaccactat gatgtctaca 2881 atgataagca caactccaaa tactctgaat ccaaatgaca tcacaaacgg ggaaattttt 2941 ctcctaatgg tattggaaca gctgtcttgt tcattaccct catcacgacc atcatcaatc 3001 tttatcgtgg gctcatctgc tgttggagag ctcgaagttt tagtgagctc tgacccctgt 3061 tcatacctca cagtcgtctc catGGTGGCg ctagcataac ttcgtataaa gtatcctata 3121 cgaagttatt tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagaa 3181 taacttcgta taatgtatgc tatacgaagt tatgaattcg atatcaagct tatcgataat 3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 3601 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 3661 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg 3841 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc 3901 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt 3961 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg 4021 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg 4081 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc 4141 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt 4201 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc 4261 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc 4321 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc 4381 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 4441 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 4501 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 4561 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 4621 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct 4681 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 4741 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 4801 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 4861 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 4921 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta 4981 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 5041 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 5101 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 5161 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 5221 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 5281 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 5341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5401 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 5461 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 5521 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 5581 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 5641 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 5701 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 5761 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 5821 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 5881 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 5941 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 6001 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 6061 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 6121 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 6181 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 6241 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 6301 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 6361 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 6421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 6481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 6841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 6901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 6961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 7021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 7081 ctggcctttt gctggccttt tgctcacatg t //