LOCUS       pAAV-hSyn-ASAP3-WPRE    5840 bp ds-DNA     circular     13-AUG-2019
DEFINITION  .
ACCESSION   urn.local...14-4c2f3f0                                             
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    complement(4167..4184)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1..141
                     /label="misc_feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      5168..5835
                     /label="rep_origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(4346..4363)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4160..5017
                     /label="misc_feature"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    839..901
                     /label="S1 (TMHMM prediction)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1957..2565
                     /label="misc_feature"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     4343..4360
                     /label="primer_bind"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3962..3978)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3103..3243
                     /label="misc_feature"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     variation       659..1936
                     /label="ASAP3"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     variation       1844..1846
                     /label="R154Q"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5257..5274)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1100..1828
                     /label="cpsfGFP-OPT145-144"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      3335..3641
                     /label="rep_origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     5235..5252
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     5793..5812
                     /label="primer_bind"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4792..4809)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1025..1087
                     /label="S3 (TMHMM prediction)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2600..2617)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3836..3853)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     variation       1898..1900
                     /label="R415Q ASAP2s mutation"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2647..2670)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     4786..4803
                     /label="primer_bind"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_signal    2585..3063
                     /label="polyA_signal"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     3909..3931
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     variation       1826..1828
                     /label="N144"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2972..2989
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     variation       1847..1909
                     /label="S4 (TMHMM prediction)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    156..640
                     /label="misc_feature"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2970..2987)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     3417..3434
                     /label="primer_bind"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    659..663
                     /label="misc_feature"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    926..988
                     /label="S2 (TMHMM prediction)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(5703..5720)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    648..651
                     /label="infusion"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3461..3478)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     source          1..5840
                     /label="source"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4029..4046)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(5536..5555)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     variation       1094..1099
                     /label="linker GT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2042..2059)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     5701..5718
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggagacgact gtgaggtatg aacaggggtc agagctcact aaaacttcga gctctccaac
      721 agcagatgag cccacgataa agattgatga tggtcgtgat gagggtaatg aacaagacag
      781 ctgttccaat accattagga gaaaaatttc cccgtttgtg atgtcatttg gattcagagt
      841 atttggagtt gtgcttatca ttgtagacat catagtggtg attgtggatc tggccatcag
      901 tgagaagaaa agaggcatta gagagattct tgaaggtgtt tccctggcta tagcactctt
      961 cttccttgtt gatgttctca tgagagtgtt tgttgaaggc ttcaagaact atttccggtc
     1021 caaactgaat actttggatg cagtcatagt agtgggcact ctgctaatta atatgaccta
     1081 ctccttctct gacggtacat ttcgcggtga taacgtgtat attaccgcgg ataaacagaa
     1141 aaacggcatt aaagcgaact ttaccgtgcg ccataacgtg gaagatggca gcgtgcagct
     1201 ggcggatcat tatcagcaga acaccccgat tggcgatggc ccggtgctgc tgccggataa
     1261 ccattatctg agcacccaga ccgtgctgag caaagatccg aacgaaaaac gcgatcacat
     1321 ggtgctgctg gaatttgtga ccgcagcggg cattacacac ggcatggatg aactgtatgg
     1381 cggcaccggc ggcagcgcga gccagggcga agaactgttt accggcgtgg tgccgattct
     1441 ggtggaactg gatggcgatg tgaacggcca taaatttagc gtgcgcggcg aaggcgaagg
     1501 cgatgcgacc attggcaaac tgaccctgaa atttatttgc accaccggca aactaccggt
     1561 gccgtggccg accctggtga ccaccttaac ctatggcgtg cagtgcttta gccgctatcc
     1621 ggatcatatg aaacgccatg atttttttaa aagcgcgatg ccggaaggct atgtgcagga
     1681 acgcaccatt agctttaaag atgatggcaa atataaaacc cgcgcggtgg tgaaatttga
     1741 aggcgatacc ctggtgaacc gcattgaact gaaaggcacc gattttaaag aagatggcaa
     1801 cattctgggg cataaactgg aatataacac agatcagatg ccgcagatgg ttactcttct
     1861 tcgagttctg agaattgtta tcttaataag aatatttcag ctggcttcac agaagaaaca
     1921 acttgaagtg gtaacctgat aagaattcga tatcaagctt atcgataatc aacctctgga
     1981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2341 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2521 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag
     2581 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     2641 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     2701 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     2761 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     2821 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     2881 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     2941 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3001 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3061 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3121 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3181 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3241 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3301 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3601 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     3661 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3721 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3841 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3901 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3961 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4021 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4081 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4141 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4201 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4261 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4321 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4381 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4441 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4501 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4561 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4621 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4681 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4741 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4801 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4861 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4921 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4981 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5041 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5281 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5761 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5821 ctggcctttt gctcacatgt
//