LOCUS       pAAV-hSyn-ASAP3Kv-WPRE  6074 bp ds-DNA     circular     13-AUG-2019
DEFINITION  .
ACCESSION   urn.local...14-4c2f3f0                                             
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    complement(4401..4418)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     4143..4165
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     3651..3668
                     /label="primer_bind"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(4263..4280)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4394..5251
                     /label="misc_feature"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     variation       659..1936
                     /label="ASAP3"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1943..1969
                     /label="GSS*3 linker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5026..5043)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      3569..3875
                     /label="rep_origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4070..4087)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     variation       1847..1909
                     /label="S4 (TMHMM prediction)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     source          1..6074
                     /label="source"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     variation       1844..1846
                     /label="R154Q"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    659..663
                     /label="misc_feature"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal    2819..3297
                     /label="polyA_signal"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5491..5508)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(5937..5954)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1025..1087
                     /label="S3 (TMHMM prediction)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2881..2904)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2191..2799
                     /label="misc_feature"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      5402..6069
                     /label="rep_origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    926..988
                     /label="S2 (TMHMM prediction)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4196..4212)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    648..651
                     /label="infusion"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    839..901
                     /label="S1 (TMHMM prediction)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     5020..5037
                     /label="primer_bind"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     variation       1826..1828
                     /label="N144"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1976..2170
                     /label="kv2.1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1..141
                     /label="misc_feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     variation       1094..1099
                     /label="linker GT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1100..1828
                     /label="cpsfGFP-OPT145-144"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     variation       1898..1900
                     /label="R415Q ASAP2s mutation"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3337..3477
                     /label="misc_feature"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2276..2293)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    156..640
                     /label="misc_feature"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(4580..4597)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     5469..5486
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2834..2851)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5770..5789)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     6027..6046
                     /label="primer_bind"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     4577..4594
                     /label="primer_bind"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3695..3712)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     3206..3223
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3204..3221)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     5935..5952
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggagacgact gtgaggtatg aacaggggtc agagctcact aaaacttcga gctctccaac
      721 agcagatgag cccacgataa agattgatga tggtcgtgat gagggtaatg aacaagacag
      781 ctgttccaat accattagga gaaaaatttc cccgtttgtg atgtcatttg gattcagagt
      841 atttggagtt gtgcttatca ttgtagacat catagtggtg attgtggatc tggccatcag
      901 tgagaagaaa agaggcatta gagagattct tgaaggtgtt tccctggcta tagcactctt
      961 cttccttgtt gatgttctca tgagagtgtt tgttgaaggc ttcaagaact atttccggtc
     1021 caaactgaat actttggatg cagtcatagt agtgggcact ctgctaatta atatgaccta
     1081 ctccttctct gacggtacat ttcgcggtga taacgtgtat attaccgcgg ataaacagaa
     1141 aaacggcatt aaagcgaact ttaccgtgcg ccataacgtg gaagatggca gcgtgcagct
     1201 ggcggatcat tatcagcaga acaccccgat tggcgatggc ccggtgctgc tgccggataa
     1261 ccattatctg agcacccaga ccgtgctgag caaagatccg aacgaaaaac gcgatcacat
     1321 ggtgctgctg gaatttgtga ccgcagcggg cattacacac ggcatggatg aactgtatgg
     1381 cggcaccggc ggcagcgcga gccagggcga agaactgttt accggcgtgg tgccgattct
     1441 ggtggaactg gatggcgatg tgaacggcca taaatttagc gtgcgcggcg aaggcgaagg
     1501 cgatgcgacc attggcaaac tgaccctgaa atttatttgc accaccggca aactaccggt
     1561 gccgtggccg accctggtga ccaccttaac ctatggcgtg cagtgcttta gccgctatcc
     1621 ggatcatatg aaacgccatg atttttttaa aagcgcgatg ccggaaggct atgtgcagga
     1681 acgcaccatt agctttaaag atgatggcaa atataaaacc cgcgcggtgg tgaaatttga
     1741 aggcgatacc ctggtgaacc gcattgaact gaaaggcacc gattttaaag aagatggcaa
     1801 cattctgggg cataaactgg aatataacac agatcagatg ccgcagatgg ttactcttct
     1861 tcgagttctg agaattgtta tcttaataag aatatttcag ctggcttcac agaagaaaca
     1921 acttgaagtg gtaaccctta agggttcttc aggttcatct ggatcttcca cgcgtcagag
     1981 ccagccgatt ctgaacacca aagaaatggc gccgcagagc aaaccgccgg aagaactgga
     2041 aatgagcagc atgccgagcc cggtggcgcc gctgccggcg cgcaccgaag gcgtgattga
     2101 tatgcgcagc atgagcagca ttgatagctt tattagctgc gcgaccgatt ttccggaagc
     2161 gacccgcttt tgataagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2221 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2281 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2341 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2401 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2461 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2521 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2581 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2641 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2701 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2761 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     2821 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2881 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2941 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3001 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3061 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3121 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3181 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3241 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3301 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3361 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3421 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3481 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3541 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     3781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     3901 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3961 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4021 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4081 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4141 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4201 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4261 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4621 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6061 ttttgctcac atgt
//