LOCUS       Exported                6289 bp ds-DNA     circular SYN 10-AUG-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Aug 10, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        117..1012
                     /label=UBI1ZM PRO
                     /label=UBI1ZM\PRO
                     /note="Maize ubiquitin promoter"
                     /note="/vntifkey=30"
                     /db_xref="VRFEATURE:F000004"
                     /db_xref="VRROLE:promoter"
     5'UTR           1013..1094
                     /label=UBI1ZM 5UTR (PHI)
                     /label=UBI1ZM\5UTR\(PHI)
                     /note="/vntifkey=52"
                     /db_xref="VRFEATURE:F000015"
                     /db_xref="VRROLE:five_Prime_UTR"
     intron          1095..2107
                     /label=UBI1ZM INTRON1 (PHI)
                     /label=UBI1ZM\INTRON1\(PHI)
                     /note="/vntifkey=15"
                     /db_xref="VRFEATURE:F000030"
                     /db_xref="VRROLE:intron"
     CDS             2126..2716
                     /codon_start=1
                     /label=CasB (Maize Optimized)
                     /label=Cse2 (MO)
                     /label=Cse2\(MO)
                     /note="/vntifkey=4"
                     /translation="MSQHTKQTVWTVTKKIIFKLSNQKEETYDKATLAKVRKTLGKPLS
                     EATEIWPILFENLPEEFLSSYQQPSYEELAIYTALQLYALHQQGVSTSVMLDDSKPYEN
                     IGSALGKLRKEDDTTSIDRRFNTMITSSTFEELTYHLRHLISLLKVESPVIQVDYSGLA
                     NDLYWFLNNSQENVRLNWARAYYKQIKGEKNNDN"
     CDS             2717..2749
                     /codon_start=1
                     /label=NLS
                     /note="/vntifkey=4"
                     /translation="SRADPKKKRKV"
     terminator      2773..3077
                     /label=PINII TERM-V3
                     /label=PINII\TERM-V3
                     /note="Modified PINII TERM with 5 nt removed from the 5' 
                     end"
                     /note="/vntifkey=43"
                     /db_xref="VRFEATURE:F002606"
                     /db_xref="VRROLE:terminator"
     CDS             4356..5171
                     /codon_start=1
                     /label=KAN-V3
                     /note="Kanamycin resistance coding sequence"
                     /note="/vntifkey=4"
                     /db_xref="VRFEATURE:F003454"
                     /db_xref="VRROLE:CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
       61 atacgactca ctatagggcg aattgggtta cccggaccga agcttgcatg cctgcagtgc
      121 agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa
      181 aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac
      241 atatatttaa actttactct acgaataata taatctatag tactacaata atatcagtgt
      301 tttagagaat catataaatg aacagttaga catggtctaa aggacaattg agtattttga
      361 caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa
      421 tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt
      481 taatggtttt tatagactaa tttttttagt acatctattt tattctattt tagcctctaa
      541 attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga
      601 ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga
      661 aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa
      721 cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc
      781 atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc
      841 gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg
      901 cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg
      961 ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac
     1021 ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc
     1081 acctccgctt caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat
     1141 cggcgttccg gtccatgcat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1201 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1261 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1321 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1381 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1441 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1501 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1561 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1621 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1681 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1741 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1801 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1861 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1921 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1981 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     2041 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     2101 tctgcaggtc gactctagag gatccatgag ccagcatact aaacagaccg tctggaccgt
     2161 caccaagaag atcatcttca agctcagcaa ccaaaaggag gaaacctacg acaaggccac
     2221 cctcgccaag gtcagaaaga ccctcggcaa gcccctcagc gaggccaccg agatctggcc
     2281 catcctcttc gagaacctcc ccgaggagtt cctcagcagc taccagcaac caagctacga
     2341 ggagctcgcc atctacaccg ccctccagct ctacgccctc caccagcagg gcgtcagcac
     2401 ctccgtcatg ctggacgaca gcaagcccta tgaaaatata ggcagcgccc tcgggaagct
     2461 gaggaaggag gacgacacca ccagcatcga caggaggttc aacaccatga tcaccagcag
     2521 caccttcgag gagctcacct accacctcag gcacctcatc agcctcctca aggtcgagtc
     2581 ccccgtcatc caggtcgact acagcggcct cgccaacgac ctctactggt tcctcaataa
     2641 tagccaggag aacgtcaggc tcaactgggc ccgcgcctac tacaagcaaa ttaaaggcga
     2701 gaagaacaac gacaacagcc gcgccgaccc caagaagaag aggaaggtct agtcttcgta
     2761 agttaaccta gacttgtcca tcttctggat tggccaactt aattaatgta tgaaataaaa
     2821 ggatgcacac atagtgacat gctaatcact ataatgtggg catcaaagtt gtgtgttatg
     2881 tgtaattact agttatctga ataaaagaga aagagatcat ccatatttct tatcctaaat
     2941 gaatgtcacg tgtctttata attctttgat gaaccagatg catttcatta accaaatcca
     3001 tatacatata aatattaatc atatataatt aatatcaatt gggttagcaa aacaaatcta
     3061 gtctaggtgt gttttgcgaa tgcggccgcc accgcggtgg agctcgaatt ccggtccggg
     3121 tcacccagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
     3181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3241 ataaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatggagc cacgttgtgt
     4261 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     4321 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     4381 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     4441 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     4501 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     4561 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     4621 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     4681 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     4741 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     4801 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     4861 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     4921 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     4981 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     5041 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     5101 agaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     5161 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     5221 gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     5281 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     5341 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
     5401 ggcgattcag gcctggtatg agtcagcaac accttcttca cgagccatga cattaaccta
     5461 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     5521 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5581 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     5641 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     5701 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     5761 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5821 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5881 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5941 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6001 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     6061 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     6121 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     6181 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6241 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaag
//
