LOCUS       Exported                6763 bp ds-DNA     circular SYN 10-AUG-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Aug 10, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        97..992
                     /label=UBI1ZM PRO
                     /label=UBI1ZM\PRO
                     /note="Maize ubiquitin promoter"
                     /note="/vntifkey=30"
                     /db_xref="VRFEATURE:F000004"
                     /db_xref="VRROLE:promoter"
     5'UTR           993..1074
                     /label=UBI1ZM 5UTR (PHI)
                     /label=UBI1ZM\5UTR\(PHI)
                     /note="/vntifkey=52"
                     /db_xref="VRFEATURE:F000015"
                     /db_xref="VRROLE:five_Prime_UTR"
     intron          1075..2087
                     /label=UBI1ZM INTRON1 (PHI)
                     /label=UBI1ZM\INTRON1\(PHI)
                     /note="/vntifkey=15"
                     /db_xref="VRFEATURE:F000030"
                     /db_xref="VRROLE:intron"
     CDS             2106..3170
                     /codon_start=1
                     /label=CasC (Maize Optimized)
                     /label=Cas7 (MO)
                     /label=Cas7\(MO)
                     /note="/vntifkey=4"
                     /translation="MTTEQRLFLDIHAIQSLPPSNMNRDDTGSPKTAQYGGVRRSRVSS
                     QAWKKAMREYFNTHGDHSNVGIRTKEIVRYVADKIVELDSSISIEDALEKADKVLIAAG
                     IKKKGEVKALYFMGDSQAKKLAQAAYDNITHKKELQKLANEDPAIDIALFGRMVAEDPT
                     LNEDSSSQVAHAISTHPVQTEFDFFTAVDDLAPEDNAGAGMLGTVEFNSSTLYRYANVA
                     IHELSKQLGNKESLENSLSLFIEAFAKSLPTGKVNTFANQTLPQTLIVTVRSDRPVNLV
                     SAFEEPVKSIEGYVTKSIDKLAKEFIKVEKFVDKPLMTFYVTLHESDSLKQIGEEKDSV
                     SKLLEDFSEMIAQYI"
     CDS             3171..3203
                     /codon_start=1
                     /label=NLS
                     /note="/vntifkey=4"
                     /translation="SRADPKKKRKV"
     terminator      3227..3531
                     /label=PINII TERM-V3
                     /label=PINII\TERM-V3
                     /note="Modified PINII TERM with 5 nt removed from the 5' 
                     end"
                     /note="/vntifkey=43"
                     /db_xref="VRFEATURE:F002606"
                     /db_xref="VRROLE:terminator"
     CDS             4810..5625
                     /codon_start=1
                     /label=KAN-V3
                     /note="Kanamycin resistance coding sequence"
                     /note="/vntifkey=4"
                     /db_xref="VRFEATURE:F003454"
                     /db_xref="VRROLE:CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
       61 aattgggtta cccggaccga agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
      121 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
      181 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
      241 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
      301 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
      361 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
      421 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
      481 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
      541 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
      601 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
      661 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
      721 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
      781 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
      841 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
      901 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
      961 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     1021 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     1081 cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg gtccatgcat
     1141 ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag
     1201 atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc
     1261 taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg
     1321 gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta
     1381 tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg
     1441 gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa
     1501 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     1561 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     1621 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     1681 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     1741 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     1801 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     1861 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     1921 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     1981 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     2041 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc gactctagag
     2101 gatccatgac caccgagcag cgcctcttcc tcgacatcca cgccatccag agcctccccc
     2161 ccagcaacat gaacagggac gacaccggca gccccaagac cgcccagtac ggcggcgtca
     2221 ggaggagcag agtctcgtcc caagcatgga agaaggccat gagggagtac ttcaacaccc
     2281 acggcgacca ctcaaacgtc ggcatccgca ccaaggagat cgtccgctac gtcgccgata
     2341 aaatagtcga gctcgacagc agcatcagca tcgaggacgc cctcgagaag gcggacaagg
     2401 tcctcatcgc cgccggaatc aagaagaagg gcgaggtcaa ggccctctac ttcatgggcg
     2461 acagccaggc caagaagctc gcccaggccg cctacgacaa tataacacat aaaaaagaac
     2521 tccagaagct cgcgaacgag gaccccgcca tcgacatcgc cctcttcggc aggatggtcg
     2581 ccgaggaccc caccctgaac gaggactcca gcagccaggt cgcccacgcg atcagcaccc
     2641 accccgtcca gacggagttc gacttcttca ccgccgtcga cgacctcgcc cccgaggaca
     2701 acgccggcgc cggcatgctc ggcacggtcg agttcaacag cagcacgctc tacaggtacg
     2761 ccaacgtcgc catccacgag ctcagcaagc agctcggcaa caaggagagc ctcgagaaca
     2821 gcctcagcct cttcatcgag gccttcgcca agagcctccc caccggcaag gtcaatacat
     2881 ttgccaatca aaccctcccc cagaccctca tcgtcaccgt gaggagcgac aggcccgtca
     2941 acctcgtctc cgcgttcgaa gagcccgtca aatcaataga gggctacgtc accaagagca
     3001 tcgacaagct cgccaaggag ttcatcaagg tcgagaagtt cgtcgacaag cccctcatga
     3061 ccttctacgt caccctccac gagagcgaca gcctcaagca gatcggcgag gagaaggaca
     3121 gcgtcagcaa gctcctcgag gacttcagcg agatgatcgc ccagtacatc agccgcgccg
     3181 accccaagaa gaagcgcaag gtctagtctt cgtaagttaa cctagacttg tccatcttct
     3241 ggattggcca acttaattaa tgtatgaaat aaaaggatgc acacatagtg acatgctaat
     3301 cactataatg tgggcatcaa agttgtgtgt tatgtgtaat tactagttat ctgaataaaa
     3361 gagaaagaga tcatccatat ttcttatcct aaatgaatgt cacgtgtctt tataattctt
     3421 tgatgaacca gatgcatttc attaaccaaa tccatataca tataaatatt aatcatatat
     3481 aattaatatc aattgggtta gcaaaacaaa tctagtctag gtgtgttttg cgaatgcggc
     3541 cgccaccgcg gtggagctcg aattccggtc cgggtcaccc agcttgagta ttctatagtg
     3601 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3661 gctcacaatt ccacacaaca tacgagccgg aagcataaag cataaagtgt aaagcctggg
     3721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4681 gttaagggat tttggtcatg gagccacgtt gtgtctcaaa atctctgatg ttacattgca
     4741 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     4801 aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
     4861 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     4921 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     4981 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     5041 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     5101 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     5161 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     5221 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     5281 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     5341 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     5401 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     5461 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     5521 gagttttctc cttcattaca gaaacggctt tttcagaaat atggtattga taatcctgat
     5581 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     5641 tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa
     5701 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
     5761 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
     5821 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag
     5881 caacaccttc ttcacgagcc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5941 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6001 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6061 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6121 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6181 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6241 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6301 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6361 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6421 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6481 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6541 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6601 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     6661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6721 ggatgtgctg caaggcgatt aagttgggta acgccagggt ttt
//
