LOCUS       Exported                4438 bp ds-DNA     circular SYN 12-AUG-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    PV384_PHP68813/GACT-Spyn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 12, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        162..1161
                     /label=ZM-U6 POLIII CHR8 PRO
                     /label=ZM-U6\POLIII\CHR8\PRO
                     /note="/vntifkey=30"
     misc_feature    1162..1179
                     /label=Cas9 DsRed Reporter sgRNA Target
                     /label=Cas9 DsRed Reporter Targeting Spacer
                     /label=DsRed Reporter Targeting Spacer
                     /label=GACT-Spyn
                     /note="/vntifkey=21"
     misc_feature    1180..1256
                     /label=Cas9 sgRNA
                     /label=GUIDE RNA
                     /label=GUIDE\RNA
                     /note="/vntifkey=21"
     terminator      1257..1263
                     /label=ZM-U6 POLIII CHR8 TERM
                     /label=ZM-U6\POLIII\CHR8\TERM
                     /note="/vntifkey=43"
     promoter        complement(1719..1738)
                     /label=T3 PRO
                     /label=T3\PRO
                     /note="/vntifkey=30"
                     /db_xref="VRFEATURE:F004862"
                     /db_xref="VRROLE:promoter"
     rep_origin      complement(1975..2874)
                     /direction=LEFT
                     /label=COLE1 ORI
                     /label=COLE1\ORI
                     /note="/vntifkey=33"
                     /db_xref="VRFEATURE:F000006"
                     /db_xref="VRROLE:origin_of_replication"
     CDS             complement(2920..3780)
                     /codon_start=1
                     /label=AMP
                     /note="/vntifkey=4"
                     /db_xref="VRFEATURE:F000003"
                     /db_xref="VRROLE:CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3911..4366)
                     /direction=LEFT
                     /label=F1 ORI
                     /label=F1\ORI
                     /note="REPLICON; PARENT SOURCE=7770"
                     /note="/vntifkey=33"
                     /db_xref="VRFEATURE:F000456"
                     /db_xref="VRROLE:origin_of_replication"
ORIGIN
        1 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
       61 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg
      121 gtaccgggcc ccccctcgag gtcgacggta tcgataagct ttgagagtac aatgatgaac
      181 ctagattaat caatgccaaa gtctgaaaaa tgcaccctca gtctatgatc cagaaaatca
      241 agattgcttg aggccctgtt cggttgttcc ggattagagc cccggattaa ttcctagccg
      301 gattacttct ctaatttata tagattttga tgagctggaa tgaatcctgg cttattccgg
      361 tacaaccgaa caggccctga aggataccag taatcgctga gctaaattgg catgctgtca
      421 gagtgtcagt attgcagcaa ggtagtgaga taaccggcat catggtgcca gtttgatggc
      481 accattaggg ttagagatgg tggccatggg cgcatgtcct ggccaacttt gtatgatata
      541 tggcagggtg aataggaaag taaaattgta ttgtaaaaag ggatttcttc tgtttgttag
      601 cgcatgtaca aggaatgcaa gttttgagcg agggggcatc aaagatctgg ctgtgtttcc
      661 agctgttttt gttagcccca tcgaatcctt gacataatga tcccgcttaa ataagcaacc
      721 tcgcttgtat agttccttgt gctctaacac acgatgatga taagtcgtaa aatagtggtg
      781 tccaaagaat ttccaggccc agttgtaaaa gctaaaatgc tattcgaatt tctactagca
      841 gtaagtcgtg tttagaaatt atttttttat ataccttttt tccttctatg tacagtagga
      901 cacagtgtca gcgccgcgtt gacggagaat atttgcaaaa aagtaaaaga gaaagtcata
      961 gcggcgtatg tgccaaaaac ttcgtcacag agagggccat aagaaacatg gcccacggcc
     1021 caatacgaag caccgcgacg aagcccaaac agcagtccgt aggtggagca aagcgctggg
     1081 taatacgcaa acgttttgtc ccaccttgac taatcacaag agtggagcgt accttataaa
     1141 ccgagccgca agcaccgaat tgaggacaga aagaaaatcg ttttagagct agaaatagca
     1201 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     1261 ttttgcggcc gcgaattcct gcagggccct cttgtcggac cagttgccca ccacgttggt
     1321 gagctcggtg aggcccttca ttgagaggaa ggaggtcatg aggtgcctac cgatgtggga
     1381 cttggggccg ttcttgatgg cgaagatgga gtagggggcg ttcttcttga gggccttgtt
     1441 gtaggacctc acgaggttgt ccttgaggag ctggtactcc tgcttgttgg aggaggagtt
     1501 gccggtcctg ttcaccctct tgagcacggg ctctgagttc ctgaggaact cgtcgaggta
     1561 cacgaggggg tcgatcctgc cgcgagcgga gaagaagtag atgtgcctgg acacggaggt
     1621 cttggtctcg gtcacgaggc actggatgat cacgccgagg tacttgttct gactagttct
     1681 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaatttc
     1741 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1801 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1861 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1921 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1981 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2581 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3901 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3961 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     4021 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4081 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4141 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4201 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4261 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4321 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     4381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagc
//
