LOCUS       Exported                4432 bp ds-DNA     circular SYN 12-AUG-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Lijuan Wang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 12, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        165..1164
                     /label=ZM-U6 POLIII CHR8 PRO
                     /label=ZM-U6\POLIII\CHR8\PRO
                     /note="/vntifkey=30"
     misc_feature    1165..1192
                     /label=CRISPR Repeat
                     /label=Repeat
                     /note="/vntifkey=21"
     misc_feature    1193..1225
                     /label=SthCascade DsRed crRNA Target
                     /label=DsRed Reporter Targeting Spacer
                     /label=Spacer
                     /note="/vntifkey=21"
     misc_feature    1226..1253
                     /label=CRISPR Repeat
                     /label=repeat
                     /note="/vntifkey=21"
     terminator      1254..1261
                     /label=ZM-U6 POLIII CHR8 TERM
                     /label=ZM-U6\POLIII\CHR8\TERM
                     /note="/vntifkey=43"
     promoter        complement(1716..1735)
                     /label=T3 PRO
                     /label=T3\PRO
                     /note="/vntifkey=30"
                     /db_xref="VRFEATURE:F004862"
                     /db_xref="VRROLE:promoter"
     rep_origin      complement(1972..2871)
                     /direction=LEFT
                     /label=COLE1 ORI
                     /label=COLE1\ORI
                     /note="/vntifkey=33"
                     /db_xref="VRFEATURE:F000006"
                     /db_xref="VRROLE:origin_of_replication"
     CDS             complement(2917..3777)
                     /codon_start=1
                     /label=AMP
                     /note="/vntifkey=4"
                     /db_xref="VRFEATURE:F000003"
                     /db_xref="VRROLE:CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3908..4363)
                     /direction=LEFT
                     /label=F1 ORI
                     /label=F1\ORI
                     /note="REPLICON; PARENT SOURCE=7770"
                     /note="/vntifkey=33"
                     /db_xref="VRFEATURE:F000456"
                     /db_xref="VRROLE:origin_of_replication"
ORIGIN
        1 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
       61 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
      121 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gctttgagag tacaatgatg
      181 aacctagatt aatcaatgcc aaagtctgaa aaatgcaccc tcagtctatg atccagaaaa
      241 tcaagattgc ttgaggccct gttcggttgt tccggattag agccccggat taattcctag
      301 ccggattact tctctaattt atatagattt tgatgagctg gaatgaatcc tggcttattc
      361 cggtacaacc gaacaggccc tgaaggatac cagtaatcgc tgagctaaat tggcatgctg
      421 tcagagtgtc agtattgcag caaggtagtg agataaccgg catcatggtg ccagtttgat
      481 ggcaccatta gggttagaga tggtggccat gggcgcatgt cctggccaac tttgtatgat
      541 atatggcagg gtgaatagga aagtaaaatt gtattgtaaa aagggatttc ttctgtttgt
      601 tagcgcatgt acaaggaatg caagttttga gcgagggggc atcaaagatc tggctgtgtt
      661 tccagctgtt tttgttagcc ccatcgaatc cttgacataa tgatcccgct taaataagca
      721 acctcgcttg tatagttcct tgtgctctaa cacacgatga tgataagtcg taaaatagtg
      781 gtgtccaaag aatttccagg cccagttgta aaagctaaaa tgctattcga atttctacta
      841 gcagtaagtc gtgtttagaa attatttttt tatatacctt ttttccttct atgtacagta
      901 ggacacagtg tcagcgccgc gttgacggag aatatttgca aaaaagtaaa agagaaagtc
      961 atagcggcgt atgtgccaaa aacttcgtca cagagagggc cataagaaac atggcccacg
     1021 gcccaatacg aagcaccgcg acgaagccca aacagcagtc cgtaggtgga gcaaagcgct
     1081 gggtaatacg caaacgtttt gtcccacctt gactaatcac aagagtggag cgtaccttat
     1141 aaaccgagcc gcaagcaccg aattgttttt cccgcacacg cgggggtgat ccttctaaac
     1201 gctaaagagg aagaggacag aaagagtttt tcccgcacac gcgggggtga tccttttttt
     1261 tgcggccgcg aattcctgca gggccctctt gtcggaccag ttgcccacca cgttggtgag
     1321 ctcggtgagg cccttcattg agaggaagga ggtcatgagg tgcctaccga tgtgggactt
     1381 ggggccgttc ttgatggcga agatggagta gggggcgttc ttcttgaggg ccttgttgta
     1441 ggacctcacg aggttgtcct tgaggagctg gtactcctgc ttgttggagg aggagttgcc
     1501 ggtcctgttc accctcttga gcacgggctc tgagttcctg aggaactcgt cgaggtacac
     1561 gagggggtcg atcctgccgc gagcggagaa gaagtagatg tgcctggaca cggaggtctt
     1621 ggtctcggtc acgaggcact ggatgatcac gccgaggtac ttgttctgac tagttctaga
     1681 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag
     1741 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1801 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1861 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1921 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1981 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2041 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2101 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2161 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2221 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2341 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2461 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2521 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2581 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2641 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2701 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2761 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2821 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3061 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3121 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3181 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3241 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3301 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3361 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3421 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3481 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3541 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3601 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3661 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3721 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3781 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3841 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3901 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3961 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4021 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4081 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4141 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4201 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4261 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4321 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4381 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg cc
//
