LOCUS       pUBQ_AVRa1              7626 bp    DNA     circular     14-AUG-2019
DEFINITION  GW cloning(LR): pENTR-MLA10  x  pUbiGW-mYFP 
ACCESSION   pUbi-MLA10
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     terminator      complement(76..360)
                     /dnas_title="Tnos"
                     /gene="Tnos"
     CDS             complement(367..918)
                     /dnas_title="pat"
                     /gene="BASTA resistance"
     promoter        complement(940..1250)
                     /dnas_title="Pnos"
                     /gene="Pnos"
     promoter        1253..3216
                     /dnas_title="'pZmUBQ"
                     /gene="'pZmUBQ"
                     /product="Ubiquitin promoter"
     CDS             3649..4368
                     /dnas_title="YFP"
                     /gene="YFP"
     terminator      4374..4590
                     /dnas_title="term"
                     /gene="term"
                     /product="35S terminator"
     misc_feature    complement(4661..4686)
                     /dnas_title="RB"
                     /gene="RB"
                     /product="right border"
     oriT            4801..5509
                     /dnas_title="RK2 ori"
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
     CDS             5672..6532
                     /dnas_title="bla"
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin
                     resistance A.tumefaciens"
     oriT            6601..7293
                     /dnas_title="ColE1 ori"
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
     misc_feature    3274..3298
                     /dnas_title="attB1"
                     /gene="attB1"
                     /note="recognized as attB1"
     misc_feature    3617..3641
                     /dnas_title="attB2"
                     /gene="attB2"
                     /note="recognized as attB2"
     misc_feature    7603..7626
                     /note="LB"
                     /note="left border"
     CDS             3319..3600
                     /note="AVRa1 %E2%88%86SP with STOP"
     source          1..7626
                     /dnas_title="pUBQ_AVRa1"
ORIGIN      
        1 CAAATTGACG CTTAGACAAC TTAATAACAC ATTGCGGACG TTTTTAATGA TCGAATACTA
       61 ACGTCTCTAC CAGATATCAG CTTGCATGCC GGTCGATCTA GTAACATAGA TGACACCGCG
      121 CGCGATAATT TATCCTAGTT TGCGCGCTAT ATTTTGTTTT CTATCGCGTA TTAAATGTAT
      181 AATTGCGGGA CTCTAATCAT AAAAACCCAT CTCATAAATA ACGTCATGCA TTACATGTTA
      241 ATTATTACAT GCTTAACGTA ATTCAACAGA AATTATATGA TAATCATCGC AAGACCGGCA
      301 ACAGGATTCA ATCTTAAGAA ACTTTATTGC CAAATGTTTG AACGATCTGC TTGACTCTAG
      361 GGGTCATCAG ATTTCGGTGA CGGGCAGGAC CGGACGGGGC GGCACCGGCA GGCTGAAGTC
      421 CAGCTGCCAG AAACCCACGT CATGCCAGTT CCCGTGCTTG AAGCCGGCCG CCCGCAGCAT
      481 GCCGCGGGGG GCATATCCGA GCGCCTCGTG CATGCGCACG CTCGGGTCGT TGGGCAGCCC
      541 GATGACAGCG ACCACGCTCT TGAAGCCCTG TGCCTCCAGG GACTTCAGCA GGTGGGTGTA
      601 GAGCGTGGAG CCCAGTCCCG TCCGCTGGTG GCGGGGGGAG ACGTACACGG TCGACTCGGC
      661 CGTCCAGTCG TAGGCGTTGC GTGCCTTCCA GGGGCCCGCG TAGGCGATGC CGGCGACCTC
      721 GCCGTCCACC TCGGCGACGA GCCAGGGATA GCGCTCCCGC AGACGGACGA GGTCGTCCGT
      781 CCACTCCTGC GGTTCCTGCG GCTCGGTACG GAAGTTGACC GTGCTTGTCT CGATGTAGTG
      841 GTTGACGATG GTGCAGACCG CCGGCATGTC CGCCTCGGTG GCACGGCGGA TGTCGGCCGG
      901 GCGTCGTTCT GGGCTCATGG TAGATCCCCC TCGATCGAGT TGAGAGTGAA TATGAGACTC
      961 TAATTGGATA CCGAGGGGAA TTTATGGAAC GTCAGTGGAG CATTTTTGAC AAGAAATATT
     1021 TGCTAGCTGA TAGTGACCTT AGGCGACTTT TGAACGCGCA ATAATGGTTT CTGACGTATG
     1081 TGCTTAGCTC ATTAAACTCC AGAAACCCGC GGCTGAGTGG CTCCTTCAAC GTTGCGGTTC
     1141 TGTCAGTTCC AAACGTAAAA CGGCTTGTCC CGCGTCATCG GCGGGGGTCA TAACGTGACT
     1201 CCCTTAATTC TCCGCTCATG ATCAGATTGT CGTTTCCCGC CTTCGGTTTG GGCGCGCCGA
     1261 TTTAATGAGC ATTGCATGTC TAAGTTATAA AAAATTACCA CATATTTTTT TGTCACACTT
     1321 GTTTGAAGTG CAGTTTATCT ATCTTTATAC ATATATTTAA ACTTCACTCT ACGAATAATA
     1381 TAATCTATAG TACTACAATA ATATCAGTGT TTTAGAGAAT CATATAAATG AACAGTTAGA
     1441 CATGGTCTAA AGGACAATTG AGTACTTTGA CAACAGGACT CTACAGTTTT ATCTCTTTAG
     1501 TGTGCATGTG TTCTCCTTTT TTTTTGCAAA TAGCTTCACC TATATAATAC TTCATCCATT
     1561 TTATTAGTAC ATCCATTTAG GGTTTAGGGT TAATGGTTTT TATAGACTAA TTTTTTTAGT
     1621 ACATCTATTT TATTCTATTT TAGCCTCTAA ATTAAGAAAA CTAAAACTCT ATTTTAGTTT
     1681 TTTTATTTAA TAATTTAGAT ATAAAATAGA ATAAAATAAA GTGACTAAAA ATTAAACAAA
     1741 TACCCTTTAA GAAATTAAAA AAACTAAGGA AACATTTTTC TTGTTTCGAG TAGATAATGC
     1801 CAGCCTGTTA AACGCCGTCG ACGAGTCTAA CGGACACCAA CCAGCGAACC AGCAGCGTCG
     1861 CGTCGGGCCA AGCGAAGCAG ACGGCACGGC ATCTCTGTCG CTGCCTCTGG GCCCCTCTCG
     1921 AGAGTTCCGC TCCACCGTTG GACTTGCTCC GCTGTCGGCA TCCAGAAATT GCGTGGCGGA
     1981 GCGGCAGACG TGAGCCGGCA CGGCAGGCGG CCTCCTCCTC CTCTCACGGC ACCGGCAGCT
     2041 ACGGGGGATT CCTTTCCCAC CGCTCCTTCG CTTTCCCTTC CTCGCCCGCC GTAATAAATA
     2101 GACACCCCCT CCACACCCTC TTTCCCCAAC CTCGTGTTGT TCGGAGCGCA CACACACACA
     2161 ACCAGATCTC CCCCAAATCC ACCCGTCGGC ACCTCCGCTT CAAGGTACGC CGCTCGTCCT
     2221 CCCCCCCCCC CTCTCTACCT TCTCTAGATC GGCGTTCCGG TCCATGGTTA GGGCCCGGTA
     2281 GTTCTACTTC TGTTCATGTT TGTGTTAGAT CCGTGTTTGT GATAGATCCG TGCTGCTAGC
     2341 GTTCGTACAC GGATGCGACC TGTACGTCAG ACACGTTCTG ATTGCTAACT TGCCAGTGTT
     2401 TCTCTTTGGG GAATCCTGGG ATGGCTCTAG CCGTTCCGCA GACGGGATCG ATTTCATGAT
     2461 TTTTTTTTGT TTCGTTGCAT AGGGTTTGGT TTGCCCTTTT CCTTTATTTC AATATATGCC
     2521 GTGCACTTGT TTGTCGGGTC ATCTTTTCAT GTTTTTTTTT TGGCTTGGTT GTGATGATGT
     2581 GGTCTGGTTG GGCGGTCGTT CTAAATCGGA GTAGAATTCT GTTTCAAACT ACCTGGTGGA
     2641 TTTATTAATT TTGGATCTGT ATGTGTGTGC CATACATATT CATAGTTACG AATTGAAGAT
     2701 GATGGATGGA AATATCGATC TAGGATAGGT ATACATGTTG ATGCGGGTTT TACTGATGCA
     2761 TATACAGAGA TGCTTTTTGT TCGCTTGGTT GTGATGATGT GGTGTGGTTG GGCGGTCGTT
     2821 CATTCGTTCT AGATCGGAGT AGAATACTGT TTCAAACTAA CTGGTGGATT TATTAATTTT
     2881 GGATCTGTAT GTGTGTGCCA TACATCTTCA TAGTTACGAG TTTAAGATGA TGGATGGAAG
     2941 TATCGATCTA GGATAGGTAT ACATGTTGAT GTGGGTTTTA CTGATGCATA TACATGATGG
     3001 CATATGCAGC ATCTATTCAT ATGCTCTAAC CTTGAGTACC TATCTATTAT AATAAACAAG
     3061 TATGTTTTAT AATTATTTTG ATCTTGATAT ACTTGGATGA TGGCATATGC AGCAGCTATA
     3121 TGTGGATTTT TTTAGCCCTG CCTTCATACG CTATTTATTT GCTTGGTACT GTTTCTTTTG
     3181 TCGATGCTCA CCCTGTTGTT TGGTGTTACT TCTGCAAATC CCTCGAGGTC GACGGTATCG
     3241 ATAAGCTTGA TATCGAATTC CTGCAGCCCA TCAACAAGTT TGTACAAAAA AGCAGGCTCC
     3301 GCGGCCGCCC CCTTCACCAT GCGAACGTGG CAATGCCCAA GCGGTGACAT ACTTTATGAA
     3361 GAAGATGTTT ATTCACATAC AAATATGAAT TCCTTTGAAC ACTTTCCGTT TAAAACTATA
     3421 GAGGATGAAG TCTATGAAAA CACTAGTTTG AGTCGCGGTG TCGTTTACAA GGTATTGGAT
     3481 GTGCATCCTT CGTCATCTAG CGCAACAACT TTTGCTGTCT TTACTTCAAA TAAACGAGTG
     3541 GCAGTAGTAT ACCAACAGGG CTATCGAAAT AACCGAAAAT TCATTGAAGA ATGCACCTAG
     3601 AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TGGATGGGAT GGTGAGCAAG
     3661 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
     3721 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
     3781 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
     3841 TTCGGCTACG GCGTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
     3901 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
     3961 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
     4021 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
     4081 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
     4141 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
     4201 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC
     4261 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
     4321 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGA GCTCTAGAGT
     4381 CCGCAAAAAT CACCAGTCTC TCTCTACAAA TCTATCTCTC TCTATTTTTC TCCAGAATAA
     4441 TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCTTAT AGGGTTTCGC TCATGTGTTG
     4501 AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT CAATAAAATT
     4561 TCTAATTCCT AAAACCAAAA TCCAGTGACC GGGCGGCCGC CACCGCGGTG GAGGGGGATC
     4621 AGATTGTCGT TTCCCGCCTT CAGTTTAAAC TATCAGTGTT TGACAGGATA TATTGGCGGG
     4681 TAAACCTAAG AGAAAAGAGC GTTTATTAGA ATAATCGGAT ATTTAAAAGG GCGTGAAAAG
     4741 GTTTATCCGT TCGTCCATTT GTATGTGTAC ATCACCGACG AGCAAGGCAA GACCGAGCGC
     4801 CTTTCCGACG CTCACCGGGC TGGTTGCCCT CGCCGCTGGG CTGGCGGCCG TCTATGGCCC
     4861 TGCAAACGCG CCAGAAACGC CGTCGAAGCC GTGTGCGAGA CACCGGCCGC CGGCGTTGTG
     4921 GATACCTCGC GGAAAACTTG GCCCTCACTG ACAGATGAGG GGCGGACGTT GACACTTGAG
     4981 GGGCCGACTC ACCCGGCGCG GCGTTGACAG ATGAGGGGCA GGCTCGATTT CGGCCGGCGA
     5041 CGTGGAGCTG GCCAGCCTCG CAAATCGGCG AAAACGCCTG ATTTTACGCG AGTTTCCCAC
     5101 AGATGATGTG GACAAGCCTG GGGATAAGTG CCCTGCGGTA TTGACACTTG AGGGGCGCGA
     5161 CTACTGACAG ATGAGGGGCG CGATCCTTGA CACTTGAGGG GCAGAGTGCT GACAGATGAG
     5221 GGGCGCACCT ATTGACATTT GAGGGGCTGT CCACAGGCAG AAAATCCAGC ATTTGCAAGG
     5281 GTTTCCGCCC GTTTTTCGGC CACCGCTAAC CTGTCTTTTA ACCTGCTTTT AAACCAATAT
     5341 TTATAAACCT TGTTTTTAAC CAGGGCTGCG CCCTGTGCGC GTGACCGCGC ACGCCGAAGG
     5401 GGGGTGCCCC CCCTTCTCGA ACCCTCCCGG CCCGCTAACG CGGGCCTCCC ATCCCCCCAG
     5461 GGGCTGCGCC CCTCGGCCGC GAACGGCCTC ACCCCAAAAA TGGCAGCGCT GGCAGTCCTT
     5521 GCCATTGCCG GGATCGGGGC AGTAACGGGA TGGGCGATCA GCCCGACAAG CTACCCCTAT
     5581 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA
     5641 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT
     5701 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA
     5761 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA
     5821 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT
     5881 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG
     5941 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA
     6001 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA
     6061 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT
     6121 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC
     6181 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA
     6241 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA
     6301 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC
     6361 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA
     6421 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA
     6481 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA
     6541 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT
     6601 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT
     6661 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
     6721 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     6781 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
     6841 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
     6901 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
     6961 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
     7021 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
     7081 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
     7141 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
     7201 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
     7261 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
     7321 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GGACTCTAGC TAGAGGATCA CAGGCAGCAA
     7381 CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT GTTTCAAACC
     7441 CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC ATGAGCAAAG TCTGCCGCCT
     7501 TACAACGGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA
     7561 TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG
     7621 TGTAAA
//

