LOCUS       pZmUBQ_GW_YFP           8963 bp    DNA     circular     14-AUG-2019
DEFINITION  GW cloning(LR): pENTR-MLA10  x  pUbiGW-mYFP 
ACCESSION   pUbi-MLA10
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     terminator      complement(76..360)
                     /gene="Tnos"
     CDS             complement(367..918)
                     /dnas_title="pat"
                     /gene="BASTA resistance"
     promoter        complement(940..1250)
                     /gene="Pnos"
     promoter        1253..3216
                     /gene="'pZmUBQ"
                     /product="Ubiquitin promoter"
     CDS             4986..5705
                     /gene="YFP"
     terminator      5711..5927
                     /gene="term"
                     /product="35S terminator"
     misc_feature    complement(5998..6023)
                     /gene="RB"
                     /product="right border"
     oriT            6138..6846
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
     CDS             7009..7869
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin
                     resistance A.tumefaciens"
     oriT            7938..8630
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
     misc_feature    3274..3397
                     /dnas_title="attR1"
                     /gene="attR1"
     CDS             3507..4166
                     /gene="CmR"
     CDS             4508..4813
                     /gene="ccdB"
     misc_feature    4854..4978
                     /gene="attR2"
     misc_feature    join(8940..8963,1..1)
                     /dnas_title="LB"
                     /note="Left Border"
     source          1..8963
                     /dnas_title="pZmUBQ_GW_YFP"
ORIGIN      
        1 CAAATTGACG CTTAGACAAC TTAATAACAC ATTGCGGACG TTTTTAATGA TCGAATACTA
       61 ACGTCTCTAC CAGATATCAG CTTGCATGCC GGTCGATCTA GTAACATAGA TGACACCGCG
      121 CGCGATAATT TATCCTAGTT TGCGCGCTAT ATTTTGTTTT CTATCGCGTA TTAAATGTAT
      181 AATTGCGGGA CTCTAATCAT AAAAACCCAT CTCATAAATA ACGTCATGCA TTACATGTTA
      241 ATTATTACAT GCTTAACGTA ATTCAACAGA AATTATATGA TAATCATCGC AAGACCGGCA
      301 ACAGGATTCA ATCTTAAGAA ACTTTATTGC CAAATGTTTG AACGATCTGC TTGACTCTAG
      361 GGGTCATCAG ATTTCGGTGA CGGGCAGGAC CGGACGGGGC GGCACCGGCA GGCTGAAGTC
      421 CAGCTGCCAG AAACCCACGT CATGCCAGTT CCCGTGCTTG AAGCCGGCCG CCCGCAGCAT
      481 GCCGCGGGGG GCATATCCGA GCGCCTCGTG CATGCGCACG CTCGGGTCGT TGGGCAGCCC
      541 GATGACAGCG ACCACGCTCT TGAAGCCCTG TGCCTCCAGG GACTTCAGCA GGTGGGTGTA
      601 GAGCGTGGAG CCCAGTCCCG TCCGCTGGTG GCGGGGGGAG ACGTACACGG TCGACTCGGC
      661 CGTCCAGTCG TAGGCGTTGC GTGCCTTCCA GGGGCCCGCG TAGGCGATGC CGGCGACCTC
      721 GCCGTCCACC TCGGCGACGA GCCAGGGATA GCGCTCCCGC AGACGGACGA GGTCGTCCGT
      781 CCACTCCTGC GGTTCCTGCG GCTCGGTACG GAAGTTGACC GTGCTTGTCT CGATGTAGTG
      841 GTTGACGATG GTGCAGACCG CCGGCATGTC CGCCTCGGTG GCACGGCGGA TGTCGGCCGG
      901 GCGTCGTTCT GGGCTCATGG TAGATCCCCC TCGATCGAGT TGAGAGTGAA TATGAGACTC
      961 TAATTGGATA CCGAGGGGAA TTTATGGAAC GTCAGTGGAG CATTTTTGAC AAGAAATATT
     1021 TGCTAGCTGA TAGTGACCTT AGGCGACTTT TGAACGCGCA ATAATGGTTT CTGACGTATG
     1081 TGCTTAGCTC ATTAAACTCC AGAAACCCGC GGCTGAGTGG CTCCTTCAAC GTTGCGGTTC
     1141 TGTCAGTTCC AAACGTAAAA CGGCTTGTCC CGCGTCATCG GCGGGGGTCA TAACGTGACT
     1201 CCCTTAATTC TCCGCTCATG ATCAGATTGT CGTTTCCCGC CTTCGGTTTG GGCGCGCCGA
     1261 TTTAATGAGC ATTGCATGTC TAAGTTATAA AAAATTACCA CATATTTTTT TGTCACACTT
     1321 GTTTGAAGTG CAGTTTATCT ATCTTTATAC ATATATTTAA ACTTCACTCT ACGAATAATA
     1381 TAATCTATAG TACTACAATA ATATCAGTGT TTTAGAGAAT CATATAAATG AACAGTTAGA
     1441 CATGGTCTAA AGGACAATTG AGTACTTTGA CAACAGGACT CTACAGTTTT ATCTCTTTAG
     1501 TGTGCATGTG TTCTCCTTTT TTTTTGCAAA TAGCTTCACC TATATAATAC TTCATCCATT
     1561 TTATTAGTAC ATCCATTTAG GGTTTAGGGT TAATGGTTTT TATAGACTAA TTTTTTTAGT
     1621 ACATCTATTT TATTCTATTT TAGCCTCTAA ATTAAGAAAA CTAAAACTCT ATTTTAGTTT
     1681 TTTTATTTAA TAATTTAGAT ATAAAATAGA ATAAAATAAA GTGACTAAAA ATTAAACAAA
     1741 TACCCTTTAA GAAATTAAAA AAACTAAGGA AACATTTTTC TTGTTTCGAG TAGATAATGC
     1801 CAGCCTGTTA AACGCCGTCG ACGAGTCTAA CGGACACCAA CCAGCGAACC AGCAGCGTCG
     1861 CGTCGGGCCA AGCGAAGCAG ACGGCACGGC ATCTCTGTCG CTGCCTCTGG GCCCCTCTCG
     1921 AGAGTTCCGC TCCACCGTTG GACTTGCTCC GCTGTCGGCA TCCAGAAATT GCGTGGCGGA
     1981 GCGGCAGACG TGAGCCGGCA CGGCAGGCGG CCTCCTCCTC CTCTCACGGC ACCGGCAGCT
     2041 ACGGGGGATT CCTTTCCCAC CGCTCCTTCG CTTTCCCTTC CTCGCCCGCC GTAATAAATA
     2101 GACACCCCCT CCACACCCTC TTTCCCCAAC CTCGTGTTGT TCGGAGCGCA CACACACACA
     2161 ACCAGATCTC CCCCAAATCC ACCCGTCGGC ACCTCCGCTT CAAGGTACGC CGCTCGTCCT
     2221 CCCCCCCCCC CTCTCTACCT TCTCTAGATC GGCGTTCCGG TCCATGGTTA GGGCCCGGTA
     2281 GTTCTACTTC TGTTCATGTT TGTGTTAGAT CCGTGTTTGT GATAGATCCG TGCTGCTAGC
     2341 GTTCGTACAC GGATGCGACC TGTACGTCAG ACACGTTCTG ATTGCTAACT TGCCAGTGTT
     2401 TCTCTTTGGG GAATCCTGGG ATGGCTCTAG CCGTTCCGCA GACGGGATCG ATTTCATGAT
     2461 TTTTTTTTGT TTCGTTGCAT AGGGTTTGGT TTGCCCTTTT CCTTTATTTC AATATATGCC
     2521 GTGCACTTGT TTGTCGGGTC ATCTTTTCAT GTTTTTTTTT TGGCTTGGTT GTGATGATGT
     2581 GGTCTGGTTG GGCGGTCGTT CTAAATCGGA GTAGAATTCT GTTTCAAACT ACCTGGTGGA
     2641 TTTATTAATT TTGGATCTGT ATGTGTGTGC CATACATATT CATAGTTACG AATTGAAGAT
     2701 GATGGATGGA AATATCGATC TAGGATAGGT ATACATGTTG ATGCGGGTTT TACTGATGCA
     2761 TATACAGAGA TGCTTTTTGT TCGCTTGGTT GTGATGATGT GGTGTGGTTG GGCGGTCGTT
     2821 CATTCGTTCT AGATCGGAGT AGAATACTGT TTCAAACTAA CTGGTGGATT TATTAATTTT
     2881 GGATCTGTAT GTGTGTGCCA TACATCTTCA TAGTTACGAG TTTAAGATGA TGGATGGAAG
     2941 TATCGATCTA GGATAGGTAT ACATGTTGAT GTGGGTTTTA CTGATGCATA TACATGATGG
     3001 CATATGCAGC ATCTATTCAT ATGCTCTAAC CTTGAGTACC TATCTATTAT AATAAACAAG
     3061 TATGTTTTAT AATTATTTTG ATCTTGATAT ACTTGGATGA TGGCATATGC AGCAGCTATA
     3121 TGTGGATTTT TTTAGCCCTG CCTTCATACG CTATTTATTT GCTTGGTACT GTTTCTTTTG
     3181 TCGATGCTCA CCCTGTTGTT TGGTGTTACT TCTGCAAATC CCTCGAGGTC GACGGTATCG
     3241 ATAAGCTTGA TATCGAATTC CTGCAGCCCA TCAACAAGTT TGTACAAAAA AGCTGAACGA
     3301 GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT
     3361 ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG
     3421 GCTTTACACT TTATGCTTCC GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGGCGA
     3481 GATTTTCAGG AGCTAAGGAA GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG
     3541 ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA
     3601 CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA
     3661 AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG
     3721 AATTCCGTAT GGCAATGAAA GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT
     3781 ACACCGTTTT CCATGAGCAA ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG
     3841 ATTTCCGGCA GTTTCTACAC ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG
     3901 CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA
     3961 GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA CTTCTTCGCC CCCGTTTTCA
     4021 CCATGGGCAA ATATTATACG CAAGGCGACA AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC
     4081 ATCATGCCGT CTGTGATGGC TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT
     4141 GCGATGAGTG GCAGGGCGGG GCGTAAAGAT CTGGATCCGG CTTACTAAAA GCCAGATAAC
     4201 AGTATGCGTA TTTGCGCGCT GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC
     4261 GAAGTATGTC AAAAAGAGGT GTGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA
     4321 CAGCTATCAG TTGCTCAAGG CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC
     4381 ATGCAGAATG AAGCCCGTCG TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG
     4441 ATGGCTGAGG TCGCCCGGTT TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGAC
     4501 TGGTGAAATG CAGTTTAAGG TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT
     4561 GGATGTACAG AGTGATATTA TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG
     4621 TGCACGTCTG CTGTCAGATA AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA
     4681 TGAAAGCTGG CGCATGATGA CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA
     4741 AGAAGTGGCT GATCTCAGCC ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT
     4801 CTGGGGAATA TAAATGTCAG GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG
     4861 ACTGGATATG TTGTGTTTTA CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA
     4921 ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTGG
     4981 ATGGGATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
     5041 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA
     5101 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC
     5161 CCACCCTCGT GACCACCTTC GGCTACGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA
     5221 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA
     5281 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
     5341 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG
     5401 GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA
     5461 AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC
     5521 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA
     5581 ACCACTACCT GAGCTACCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA
     5641 TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA
     5701 AGTAAGAGCT CTAGAGTCCG CAAAAATCAC CAGTCTCTCT CTACAAATCT ATCTCTCTCT
     5761 ATTTTTCTCC AGAATAATGT GTGAGTAGTT CCCAGATAAG GGAATTAGGG TTCTTATAGG
     5821 GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT TTGTAAAATA
     5881 CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTGACCGGG CGGCCGCCAC
     5941 CGCGGTGGAG GGGGATCAGA TTGTCGTTTC CCGCCTTCAG TTTAAACTAT CAGTGTTTGA
     6001 CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ATCGGATATT
     6061 TAAAAGGGCG TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGTACATC ACCGACGAGC
     6121 AAGGCAAGAC CGAGCGCCTT TCCGACGCTC ACCGGGCTGG TTGCCCTCGC CGCTGGGCTG
     6181 GCGGCCGTCT ATGGCCCTGC AAACGCGCCA GAAACGCCGT CGAAGCCGTG TGCGAGACAC
     6241 CGGCCGCCGG CGTTGTGGAT ACCTCGCGGA AAACTTGGCC CTCACTGACA GATGAGGGGC
     6301 GGACGTTGAC ACTTGAGGGG CCGACTCACC CGGCGCGGCG TTGACAGATG AGGGGCAGGC
     6361 TCGATTTCGG CCGGCGACGT GGAGCTGGCC AGCCTCGCAA ATCGGCGAAA ACGCCTGATT
     6421 TTACGCGAGT TTCCCACAGA TGATGTGGAC AAGCCTGGGG ATAAGTGCCC TGCGGTATTG
     6481 ACACTTGAGG GGCGCGACTA CTGACAGATG AGGGGCGCGA TCCTTGACAC TTGAGGGGCA
     6541 GAGTGCTGAC AGATGAGGGG CGCACCTATT GACATTTGAG GGGCTGTCCA CAGGCAGAAA
     6601 ATCCAGCATT TGCAAGGGTT TCCGCCCGTT TTTCGGCCAC CGCTAACCTG TCTTTTAACC
     6661 TGCTTTTAAA CCAATATTTA TAAACCTTGT TTTTAACCAG GGCTGCGCCC TGTGCGCGTG
     6721 ACCGCGCACG CCGAAGGGGG GTGCCCCCCC TTCTCGAACC CTCCCGGCCC GCTAACGCGG
     6781 GCCTCCCATC CCCCCAGGGG CTGCGCCCCT CGGCCGCGAA CGGCCTCACC CCAAAAATGG
     6841 CAGCGCTGGC AGTCCTTGCC ATTGCCGGGA TCGGGGCAGT AACGGGATGG GCGATCAGCC
     6901 CGACAAGCTA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
     6961 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
     7021 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
     7081 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
     7141 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
     7201 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
     7261 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
     7321 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
     7381 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
     7441 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
     7501 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
     7561 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
     7621 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
     7681 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
     7741 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
     7801 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
     7861 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT
     7921 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT
     7981 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
     8041 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
     8101 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
     8161 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
     8221 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
     8281 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     8341 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
     8401 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG
     8461 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
     8521 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
     8581 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
     8641 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGGA CTCTAGCTAG
     8701 AGGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT
     8761 CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG
     8821 AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG
     8881 CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT
     8941 GGCAGGATAT ATTGTGGTGT AAA
//

