LOCUS                  pJH2255        8677 bp ds-DNA   circular     08-JUN-2012
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
ATGCAGAAGCTGATCTCAGAGGAGGACCTGCTTCAGAAGCTTATCTCAGAGGAGGACCT, and OZM141:
TCGAAGGTCCTCCTCTGAGATAAGCTTCTGAAGCAGGTCCTCCTCTGAGATCAGCTTCTGCAT. and iserted
            into pBluescript I SK +. BamHI/KpnI fragment was taken from above,
            and fused with unc-54 37UTR (pPD49.26). THIS FRAGMENT MAY NOT BE
            CORRECT. Actual fragment is derived from pSC326, but I couldn't get
            this sequence.
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     primer_bind     complement(5805..5822)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(4090..4114)
                     /label=New Feature
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     gene            4136..4855
                     /label=EGFP
                     /ApEinfo_fwdcolor=#66ff66
                     /ApEinfo_revcolor=#ff80ff
     misc_feature    3739..3771
                     /label=New Feature(1)
                     /ApEinfo_label=New Feature
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     204..224
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      7831..8513
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     rep_origin      6034..6489
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             5893..5961
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     misc_binding    176..198
                     /label=LacO
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     CDS             7074..7733
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    complement(4880..4904)
                     /label=attB2
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     UTR             5030..5764
                     /label=unc-54 3UTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     exon            5513..5667
                     /label=aex-5 exon1
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
     misc_feature    257..3730
                     /label=Psu006
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(5174..5197)
                     /label=OZM sequencing primer reverse
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3631..3651
                     /label=OZM2675
                     /ApEinfo_fwdcolor=#ffcc66
                     /ApEinfo_revcolor=green
     misc_feature    3739..4116
                     /label=ins-4
                     /ApEinfo_fwdcolor=#fff74b
                     /ApEinfo_revcolor=green
ORIGIN
        1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
       61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
      121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
      181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAATG
      241 GCCATGGGAC GTCGACAAGC TTGCATGCct gcagcgtttc cgataccccc ttatatcagc
      301 acacattcag tcatcatacc acaaaatgtt gtgatagtga taggcaaaac taccgtattt
      361 cctctattaa tcttgcatgc aagactaatt ttcgattaac ccgtaggggt gcaagactaa
      421 tagagactgc aagactatta gaggctgaaa tactaatttt cgtatgctca ataattttgg
      481 aaattggcct attttttgta gaaacttgat accgtttaaa caaggaaaaa tacacacttt
      541 ttaatatttc atcaataatt tgaacgattt tgtgatttta agttcaattt gcccaaaaaa
      601 agacaatttt tctgacgtta ccgcaaagta atgctggtca ggcaaaaaga gcggtgcaaa
      661 aatataagag actgcaatac taatagagga aatacggtaa ttgaatttta gtgaaacttg
      721 cgacagtttt cctcattttt ttgttattcc ggcatacgag tgtggcatac gagtgaggtc
      781 atcttttgtt tcttccgttt cttcatcgct tttgaaaaaa aatgttgaag aaaacttcgt
      841 agccgatagc cggataatgt ttggcacggc gttccaacat atatcattgg aaatttttta
      901 agatttttgc gaaaaatcac attcttcacg atgagaacac gttattgaag gaatataaat
      961 caagaataac atatagttat atttctctat tactttaacg ttaaatatga gcaaatttga
     1021 gcattttgat tgcgatgaaa agcagaatcg agtcaactga aatccgttca aaaacctaag
     1081 ctcggttcgc ccgaaagtcg atttttccaa tagccgaaca gcactgcttc tttattttcc
     1141 aggtattttt ggggtgtaag aattatatct tctttgtttg ggtatttacg tttccgaatt
     1201 ccctttcttg atctgtcgct atttttcaga aaccagaaca gtttatcctt tttcatgaga
     1261 acctgaatag ctcaaaaacc cgtttaattt cttttcatca tctcatttga aactttccta
     1321 gtcttcttaa tgatttccag gctcctccta catttgtatc tcaagatgca tacactttat
     1381 ttccgctcca attcgaatca tctacttctc ttttttcttc aactttctac ttttttccat
     1441 tcttttcttt caaatgttcg acgtcttcaa gtgactgctc ttcattctcc tcatttttct
     1501 cgaatttcat tcttgtctct tttttgctca aaaatggaaa atgaaagtga cgtgagattt
     1561 ggacggcggg acacgggggc agtagaagca gcaaaaagga gagaaagagg acacaaataa
     1621 gaagaacgaa ttcaaaaata agcggagagg agctatttcc gtcaattcta cctccccaat
     1681 cttcatcaat tcggctcaat tgaatgacgt cacagggaga taaaccgttt ggatgagcgc
     1741 cgtccatcac tgacgccatc ccgtttggga caagaaaaga gaaaaaagag cacaaagttt
     1801 ttggtgacgg atcttgtcaa tcatatgaaa gttgttctga ttgattgtca gtttttttcc
     1861 tacttttttg gattctatcc acttctgaac ttttgacaag tttcaaactt tctgaatcat
     1921 tttctatgca ttttcctgga attcttttta tgtaaaatat gaaatagaat gtttttgaat
     1981 tcaagttctg cttttttctt cttttttgtt ctgttcgggc ttgggtatgc tttttttaaa
     2041 aattattttg cacatcgacc aataagtgcg caacttataa aattaattta tttttgttaa
     2101 tttttgaaat acttgtattg ctttaagtga tctgacctcg ccctgagctt tccacgtagt
     2161 tatcaaatac aaatcctcca agggtaacgt acctatatta ctgatcttta taataacttt
     2221 atcacctgtc cagttccaga ggaattctgt taagcttata ggcacagaag gagtcatttc
     2281 tgctggtttt taatgatcca aatctttatt tcaagtaaaa aactgaacac ttgcgaataa
     2341 aactatcaga ttaaccattc accaaaaatg tgtttgaatc taaaactttc tcagtattcc
     2401 aaatatagaa ataaataacc acgacattgc taaaatctgt ctgaattgtg tactccttac
     2461 cgtgaagtaa taatggatat aatgaatcgt ttgaaatgaa tgatcagcac atttttggtg
     2521 aaagatcaca aataaggaat aagcgacgga aaataaaacg attatttcgg atcaaaaatt
     2581 tgttgaagat catatacact cgagaccaag attattctag acaattttca aattggcttc
     2641 tttgtttgca aatcttcata ataatctgtg aagtttggaa atttgaattt ttaatctttt
     2701 ttcatagatt atagttttta tttctttgca aaactatatt aaaaaccgat gcattgtttt
     2761 agggaaatta atgagccttt tgttcaacac taaaaacaat aaaattaaaa ttttggcttc
     2821 atcatttgac cttttttaag ttcgaaaact tttctcgtat ttctgaaccg ccaatttttt
     2881 cacacatctc tagacttttg gtgcccgttc cagaaagtta agtaattgct attctaagaa
     2941 agttctcaac attgtttttt agttctgatt gaattctgat gttccaggaa tatattttaa
     3001 atttaatatt ctttgcacta tttctatact aactaaataa taaatagtct aagtatgttc
     3061 aatgagaaca aaaaagctct atctatattt ttctatccta tttcatttat atccttttca
     3121 ttttgaactc accagcttct cttcttcttc ttgtccatat acttcttaaa agtctcgaac
     3181 ttctctctct cactctccat caatttcttt atggctaccg cttgcgctct gccgcttccg
     3241 aagaaagaag gcgttggcag agctctcaat tctatttttt ctcgccgtag gctatcgatt
     3301 tctcagcttc tcttctctct tcctcactcc accaccacca cttcgggctt cttcttttct
     3361 tcattttcgt cttcttcttc atcctcttct ttttttttca gttccccctc actcctcccc
     3421 ttatatgcgc gtgcgagagg ggtgcaaaag cagcgcgcat ccgagaattg aaacgagaaa
     3481 acggagacgc agcagcagtt cgtcctcaga aaaatagcca gtaaaaagag aaaaagatag
     3541 agagagacct atcgcatttt attttcaaat tgcaattcct gcaattcatg tgtgcgtgtg
     3601 cccattttca attcttcccg ttatttttca gaccattacc aacgttcttt ctagatcttg
     3661 agacaatctt ccttctgctc aatcgttcgt cgtgaagacg gcatcgacga cgacgacgAT
     3721 cAGCAGTAAA ggatccccat gttttcattc tttacatatt tccttctctc cgcacttctt
     3781 ctctccgctt catgtcgaca accttccatg gacaccagca aagccgatcg tattctacga
     3841 gagatcgaaa tggaaacaga actcgaaaat caactctccc gagcacgacg agtcccagct
     3901 ggagaggttc gtgcctgtgg aagacgactt cttctctttg tctggtcaac ctgtggagaa
     3961 ccatgcacgc cacgtacgtt taatgctaaa aattttttaa aaaaccttaa tctgaattta
     4021 ttatttttca gaagaggaca tggacattgc cacagtttgc tgcacaacac agtgcactcc
     4081 atcatatata aaacaagctt gctgcccaga aaagggggat ccACCGGTCG CCACCATGGT
     4141 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
     4201 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
     4261 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
     4321 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
     4381 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
     4441 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
     4501 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
     4561 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
     4621 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
     4681 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
     4741 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
     4801 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG
     4861 CCGCACTCGA GATATCTAGA CCCAGCTTTC TTGTACAAAG TGGTTGATCT AGAGGGCCCG
     4921 CGGTTCGAAG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCGTACCGGT
     4981 TAGTAATGAG TTTATCTGAG CTCCGCATCG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC
     5041 CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG
     5101 TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAAACTTC TTCTTAATTT CTTTGTTTTT
     5161 TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT
     5221 CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA
     5281 AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT TTACTTCTGA TAAATTTTTT
     5341 TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT
     5401 ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA
     5461 TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT
     5521 AATTTTCCTG CTTTTGCTTT TTGGGGGTTT CCCCTATTGT TTGTCAAGAG TTTCGAGGAC
     5581 GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA
     5641 AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA
     5701 GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT
     5761 GTTTCCTACT AGTGGCCACG TGGGCCGTGC ACCTTAAGCT TGGCACTGGC CGTCGTTTTA
     5821 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC
     5881 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG
     5941 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
     6001 ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG
     6061 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
     6121 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC
     6181 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA
     6241 AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC
     6301 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC
     6361 TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
     6421 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT
     6481 TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC
     6541 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
     6601 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT
     6661 CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
     6721 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
     6781 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
     6841 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
     6901 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
     6961 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
     7021 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
     7081 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC
     7141 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
     7201 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
     7261 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
     7321 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
     7381 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC
     7441 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
     7501 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
     7561 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
     7621 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
     7681 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
     7741 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
     7801 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT
     7861 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     7921 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     7981 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     8041 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     8101 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     8161 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     8221 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     8281 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     8341 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     8401 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     8461 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     8521 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     8581 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
     8641 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGA  
//
