LOCUS       pGD255\-\pGGD000        3435 bp    DNA     circular     15-AUG-2019
DEFINITION  pGGD000.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12948012|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGD255 - pGGD000:(linker12aa)mVenus|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     promoter        131..159
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            201..1061
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             201..1061
                     /vntifkey="4"
                     /label=ORF\frame\3
     rep_origin      1216..1835
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        2144..2173
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    2212..2229
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(3022..3040)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3050..3072)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    3050..3193
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    3385..3407
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    2248..2280
                     /vntifkey="21"
                     /label=linker
     misc_feature    2281..3000
                     /vntifkey="21"
                     /label=Venus
BASE COUNT      833 a       933 c       889 g       780 t 
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct 
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg 
     2221 acactataga agtgaagctt ggtctcatca ggcagcggct ctggatcggc ggccgctgca 
     2281 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
     2341 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
     2401 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc 
     2461 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 
     2521 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
     2581 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
     2641 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
     2701 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac 
     2761 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc 
     2821 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
     2881 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
     2941 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 
     3001 ctgctgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac 
     3061 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     3121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 
     3181 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
     3241 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 
     3301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 
     3361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 
     3421 caccgaaacg cgcga 
//
