LOCUS       pRD134\mScarlet\        3387 bp    DNA     circular      9-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
            SerialCloner_Type=DNA
            SerialCloner_Comments=[DEFINITION]:  - [LOCUS]: pRD134\mScarlet\ - [GENE]: 636..14
            96 - [CDS]: 636..1496
            SerialCloner_Ends=0,0,,0,
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-11975681|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRD134 mScarlet pGGD000|
FEATURES             Location/Qualifiers
     5'clip          1..4
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="51" - label=overhangCTGC -  \
                     /SerialCloner_Desc=1..4 \
                     /vntifkey="51"
                     /label=overhang\CTGC
     misc_feature    385..407
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=pGEX_3_primer -  \
                     /SerialCloner_Desc=385..407 \
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    50..193
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=lacZ_a -  \
                     /SerialCloner_Desc=50..193 \
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    complement(50..72)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=M13_pUC_fwd_primer -  \
                     /SerialCloner_Desc=complement(50..72) \
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     promoter        complement(22..40)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="30" - label=T7_promoter -  \
                     /SerialCloner_Desc=complement(22..40) \
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    2647..2664
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=Sp6_primer -  \
                     /SerialCloner_Desc=2647..2664 \
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        2579..2608
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="30" - label=lac_promoter -  \
                     /SerialCloner_Desc=2579..2608 \
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      1651..2270
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="33" - label=pBR322_origin -  \
                     /SerialCloner_Desc=1651..2270 \
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             636..1496
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=ORFframe3 -  \
                     /SerialCloner_Desc=636..1496 \
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            636..1496
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="Ampicillin" - vntifkey="60" -  \
                     /SerialCloner_Desc=636..1496 \
                     /vntifkey="60"
     promoter        566..594
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="30" - label=AmpR_promoter -  \
                     /SerialCloner_Desc=566..594 \
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    2689..3387
                     /SerialCloner_Color=&hFF8040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /vntifkey="21"
                     /label=mScarlet
BASE COUNT      818 a       905 c       888 g       776 t 
ORIGIN
        1 ctgctgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac 
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 
      181 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
      241 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 
      301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 
      361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 
      421 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 
      481 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 
      541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
      601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
      661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
      721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
      781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
      841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
      901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
      961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     1021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     1081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     1141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     1201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     1261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     1321 ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca gcactggggc 
     1381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     1441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     1501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     1561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     1621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     1681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     1741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     1801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     1861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     1921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     1981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     2041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     2101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     2161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     2221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     2281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     2341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     2401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 
     2461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 
     2521 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 
     2581 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 
     2641 cgagctattt aggtgacact atagaagtga agcttggtct catcagcaat ggtgagcaag 
     2701 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac 
     2761 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 
     2821 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct 
     2881 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat 
     2941 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 
     3001 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag 
     3061 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg 
     3121 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg 
     3181 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc 
     3241 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc 
     3301 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc 
     3361 ggcggcatgg acgagctgta caagtaa 
//
