LOCUS       pGD425-\linker-m        3435 bp    DNA     circular     27-FEB-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11738394|
COMMENT     VNTDBDATE|-12704800|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGD425- linker-mTurquoise2|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    1..33
                     /vntifkey="21"
                     /label=linker
     misc_feature    1138..1160
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    803..946
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    complement(803..825)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     promoter        complement(775..793)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    3400..3417
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        3332..3361
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      2404..3023
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             1389..2249
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            1389..2249
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        1319..1347
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    34..753
                     /vntifkey="21"
                     /label=mTurquoise2
BASE COUNT      831 a       929 c       891 g       784 t 
ORIGIN
        1 tcaggcagcg gctctggatc ggcggccgct gcaatggtga gcaagggcga ggagctgttc 
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg 
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta ctttagcgac 
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc 
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc 
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc 
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
      721 atcactctcg gcatggacga gctgtacaag tagctgctga gaccgaattc tcgccctata 
      781 gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
      841 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
      901 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc 
      961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 
     1021 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
     1081 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     1141 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     1201 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     1261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     1321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     1381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     1441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     1501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     1561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     1621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     1681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     1741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     1801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     1861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     1921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     1981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     2041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     2101 gtggctctcg aggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     2161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     2221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     2281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     2341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     2401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     2521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     2581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     2641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     2701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     2761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     2821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     2881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     2941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     3001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     3061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     3121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     3181 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     3241 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     3301 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     3361 tgtgtggaat tgtgagcgga taacaatttc acacgagcta tttaggtgac actatagaag 
     3421 tgaagcttgg tctca 
//
