LOCUS       pGD431\-\pGGD000        3402 bp    DNA     circular     26-MAR-2019
DEFINITION  pGGD000.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12743606|
COMMENT     VNTDBDATE|-12743606|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGD431 - pGGD000:mRuby3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     promoter        131..159
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            201..1061
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             201..1061
                     /vntifkey="4"
                     /label=ORF\frame\3
     rep_origin      1216..1835
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        2144..2173
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    2212..2229
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2989..3007)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3017..3039)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    3017..3160
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    3352..3374
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    2254..2967
                     /vntifkey="21"
                     /label=mRuby3
BASE COUNT      861 a       837 c       877 g       827 t 
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct 
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg 
     2221 acactataga agtgaagctt ggtctcatca ggaatggtgt ctaagggcga agagctgatc 
     2281 aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa 
     2341 tgcacaggtg aaggagaagg cagaccgtac gagggaactc aaaccatgag gatcaaagtc 
     2401 atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt catgtatggc 
     2461 agccgtactt ttatcaagta cccggccgac atccctgatt tctttaaaca gtcctttcct 
     2521 gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt caccgtcacg 
     2581 caggacacca gccttgagga tggcgagctc gtctacaacg tcaaggtcag aggggtaaac 
     2641 tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc taatacagag 
     2701 atgatgtatc cagcagatgg tggtctgaga ggatacactg acatcgcact gaaagttgat 
     2761 ggtggtggcc atctgcactg caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg 
     2821 aacatcaaga tgcccggtgt ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt 
     2881 gacaatgaaa cctacgtagt gcaacgcgaa gtggcagttg ccaaatacag caaccttggt 
     2941 ggtggcatgg acgagctgta caagtaactg ctgagaccga attctcgccc tatagtgagt 
     3001 cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 
     3061 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 
     3121 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt 
     3181 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct 
     3241 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 
     3301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 
     3361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc ga 
//
