LOCUS       Exported               14151 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14151)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3435..3455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3456..6977
                     /codon_start=1
                     /label=dPguCas13b
                     /translation="TEQSERPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAY
                     SKADITNDQDVLSFKALWKNFDNDLERKSRLRSLILKHFSFLEGAAYGKKLFESKSSGN
                     KSSKNKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSAYRHSGSSELPLFD
                     GNMLQRLYNVFDVSVQRVKIDHEHNDEVDPHYHFNHLVRKGKKDRYGHNDNPSFKHHFV
                     DGEGMVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTETYQQMTNEVFCRSRISLPKL
                     KLESLRMDDWMLLDMLNELVRCPKPLYDRLREDDRACFRVPVDILPDEDDTDGGGEDPF
                     KNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKMIGEQPEDRHLTRNL
                     YGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYISQTSPHYHIEKGKIGLRFMPEGQ
                     HLWPSPEVGTTRTGRSKYAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAERVQ
                     GRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEE
                     KIRKKLQEMMADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAK
                     DASGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWE
                     SHTNILSFYRSYLRARKAFLERIGRSDRVENRPFLLLKEPKTDRQTLVAGWKGEFHLPR
                     GIFTEAVRDCLIEMGHDEVASYKEVGFMAKAVPLYFERACEDRVQPFYDSPFNVGNSLK
                     PKKGRFLSKEERAEEWERGKERFRDLEAWSYSAARRIEDAFAGIEYASPGNKKKIEQLL
                     RDLSLWEAFESKLKVRADRINLAKLKKEILEAQEHPYHDFKSWQKFERELRLVKNQDII
                     TWMMCRDLMEENKVEGLDTGTLYLKDIRPNVQEQGSLNVLNRVKPMRLPVVVYRADSRG
                     HVHKEEAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLR
                     VEYELAKYQTARVCVFELTLRLEESLLTRYPHLPDESFREMLESWSDPLLAKWPELHGK
                     VRLLIAVRNAFSANQYPMYDEAVFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKE
                     TVERIIQA"
     primer_bind     complement(6478..6497)
                     /label=dPgu-Bind-XbaI-R2
     CDS             7005..7721
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7737..8450
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             8457..9170
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             9198..9245
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9264..9329
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    9336..9903
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             9923..10522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    10541..11129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(11012..11023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11204..11437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        11642..11746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11747..12607
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12778..13366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    13777..13798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13813..13843
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13851..13867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13875..13891
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag catgcccaag aaaaagcgca aggtaacaga gcagagcgag aggccctaca
     3481 acggcaccta ctacaccctg gaagataagc acttctgggc cgcctttctg aacctggcca
     3541 gacacaacgc ctacatcacc ctgacacaca tcgaccggca gctggcctac agcaaggccg
     3601 acatcaccaa cgaccaggac gtgctgagct tcaaggccct gtggaagaac ttcgacaacg
     3661 acctggaaag aaagagccgg ctgcggagcc tgatcctgaa gcacttcagc tttctggaag
     3721 gcgccgctta cggcaagaag ctgttcgagt ctaagagcag cggcaacaag agcagcaaga
     3781 acaaagagct gaccaagaaa gagaaagagg aactgcaggc caacgctctg agcctggaca
     3841 acctgaagtc tatcctgttc gacttcctgc agaagctgaa ggacttccgg aactactaca
     3901 gcgcctaccg gcacagcgga agcagcgagc tgcctctgtt cgacggcaac atgctgcagc
     3961 ggctgtacaa cgtgttcgac gtgtccgtgc agcgcgtgaa gatcgaccac gagcacaacg
     4021 atgaggtgga ccctcactac cacttcaacc acctcgtgcg gaagggcaag aaggacagat
     4081 acggccacaa cgacaacccc agcttcaagc accacttcgt ggacggcgaa ggcatggtta
     4141 cagaagccgg cctgctgttc ttcgtgtccc tgttcctgga aaagcgggac gccatctgga
     4201 tgcagaagaa gatccggggc ttcaaaggcg gcaccgagac ataccagcag atgaccaacg
     4261 aggtgttctg ccggtccaga atcagcctgc ctaagctgaa gctggaaagc ctgcggatgg
     4321 acgactggat gctgctggac atgctgaacg aactcgtgcg gtgccccaag cctctgtacg
     4381 acagactgag agaggacgac cgggcctgct ttagagtgcc cgtggatatc ctgcctgacg
     4441 aggacgatac agatggcggc ggagaggacc ccttcaagaa taccctcgtc cggcaccagg
     4501 acagattccc ttacttcgcc ctgcggtact tcgacctgaa gaaggtgttc accagcctgc
     4561 ggttccacat cgatctgggc acctaccact ttgccatcta caagaagatg atcggcgagc
     4621 agcccgagga cagacacctg accagaaacc tgtacggctt cggccggatc caggacttcg
     4681 ccgaagaaca cagacccgag gaatggaagc ggctcgtcag agatctggac tacttcgaga
     4741 caggcgacaa gccctacatc agccagacaa gcccacacta ccacatcgag aagggaaaga
     4801 tcggcctgag attcatgccc gagggccagc atctttggcc ctctccagaa gtgggcacca
     4861 ccagaaccgg cagatctaag tacgcccagg acaagagact gaccgccgag gcctttctgt
     4921 ctgtgcacga gctgatgcct atgatgttct actacttcct gctgcgcgag aagtacagcg
     4981 aggaagtgtc tgccgagaga gtgcagggca gaatcaagcg cgtgatcgag gatgtgtacg
     5041 ccgtgtacga tgcctttgcc agggacgaga tcaacacccg ggatgagctg gatgcctgcc
     5101 tggccgataa gggcatcaga agaggccatc tgcctcgcca gatgatcgcc atcctgagcc
     5161 aagagcacaa ggacatggaa gagaagattc ggaagaaact gcaagagatg atggccgaca
     5221 ccgaccaccg gctggatatg ctggatagac agaccgaccg gaagatcaga atcggccgga
     5281 agaatgccgg actgcctaag agcggagtga tcgccgattg gctcgtgcgg gacatgatga
     5341 gatttcagcc cgtggccaag gacgccagcg gaaagcctct gaacaactcc aaggccaaca
     5401 gcaccgagta ccggatgctg cagagagccc tggctctgtt tggaggcgag aaagagaggc
     5461 tgaccccata cttccggcag atgaatctga ccggcggaaa caaccctcat ccttttctgc
     5521 acgaaactcg ctgggagagc cacaccaaca tcctgtcctt ttaccggtcc tacctgagag
     5581 cccgcaaggc cttcctcgaa aggatcggca gatccgacag agtggaaaac cggccatttc
     5641 tgctgctgaa agagcccaag accgacagac agacactggt ggctggatgg aagggcgagt
     5701 tccatctgcc aagaggcatc ttcacagagg ccgtgcggga ttgcctgatc gagatgggac
     5761 acgatgaagt ggccagctac aaagaagtgg gattcatggc caaggccgtg ccactgtact
     5821 ttgagagagc ctgcgaggac agggtgcagc ccttttacga cagccccttc aacgtgggca
     5881 acagcctgaa gcctaagaag ggcagattcc tgagcaaaga ggaacgggcc gaagagtggg
     5941 agcggggcaa agagagattc cgggatctcg aagcctggtc ctatagcgcc gccagaagaa
     6001 tcgaggacgc ctttgccggc atcgagtatg ctagccccgg aaacaagaag aaaatcgagc
     6061 agctcctgcg ggacctgagc ctgtgggaag cctttgagag caagctgaaa gtgcgggccg
     6121 accggatcaa tctggccaag ctgaagaaag aaatcctgga agcccaagaa cacccctacc
     6181 acgacttcaa gagctggcag aagttcgagc gcgagctgcg gctggtcaag aaccaggata
     6241 tcatcacctg gatgatgtgc cgcgacctga tggaagaaaa caaggtggaa ggcctggaca
     6301 ccggcacact gtacctgaag gacatcagac ccaacgtgca agagcagggc agcctgaacg
     6361 tgctgaacag agtgaagccc atgagactgc ccgtggtggt gtacagagcc gatagcagag
     6421 gccacgtgca caaagaagaa gcccctctgg ccaccgtgta catcgaggaa agagacacca
     6481 agctgctgaa gcagggcaac ttcaagtcct tcgtgaagga caggcggctg aacggcctgt
     6541 tctccttcgt tgatactggc ggactggcca tggaacagta ccccatctcc aagctgagag
     6601 tggaatacga gctggccaag taccagaccg ccagagtgtg tgtgttcgag ctgaccctga
     6661 gactggaaga gtccctgctg acccggtatc ctcatctgcc cgacgagagc ttcagagaga
     6721 tgctggaatc ttggagcgac cctctgctgg ccaaatggcc tgaactgcac ggaaaagtgc
     6781 ggctgctgat cgccgtgcgg aatgccttta gcgccaatca gtaccctatg tacgacgagg
     6841 ccgtgttcag ctccatccgg aagtacgatc ctagcagccc cgacgccatc gaagagagaa
     6901 tgggcctgaa tatcgcccac cgcctgtctg aggaagtgaa gcaggccaaa gaaaccgtgg
     6961 aacggatcat tcaggccagt ctagaggcgc taccggtcgc caccatggtg agcaagggcg
     7021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     7081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     7141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7201 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     7441 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctgtga
     7741 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7801 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7861 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7921 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     7981 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8041 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8101 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8161 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     8221 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     8281 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     8341 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8401 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aagcttgtga
     8461 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     8521 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     8581 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     8641 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     8701 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8761 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8821 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8881 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     8941 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     9001 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     9061 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     9121 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tcgaattcta
     9181 agccaggatt ccatatgaag cgacctgccg ccacaaagaa ggctggacag gctaagaaga
     9241 agaaacaacg cgtgcagcta agcgactaca aagaccatga cggtgattat aaagatcatg
     9301 acatcgatta caaggatgac gatgacaagt aaggatccct cccccccccc taacgttact
     9361 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     9421 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     9481 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     9541 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     9601 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     9661 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     9721 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     9781 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     9841 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     9901 taaggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9961 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    10021 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    10081 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    10141 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    10201 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10261 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    10321 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    10381 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    10441 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    10501 gacccgcaag cccggtgcct gacgcccatc gatagatcct aatcaacctc tggattacaa
    10561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    10621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    10681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    10741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    10801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
    10861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
    10921 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
    10981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
    11041 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
    11101 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
    11161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    11221 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
    11281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11401 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
    11461 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
    11521 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
    11581 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
    11641 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    11701 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    11761 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    11821 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    11881 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    11941 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
    12001 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
    12061 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
    12121 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
    12181 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
    12241 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
    12301 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    12361 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    12421 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    12481 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    12541 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    12601 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    12661 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    12721 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    12781 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    12841 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    12901 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    12961 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    13021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    13081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    13141 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    13201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    13261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    13321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    13381 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    13441 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    13501 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    13561 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
    13621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    13681 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    13741 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    13801 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    13861 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    13921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    13981 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    14041 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    14101 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
