LOCUS       Exported               12702 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3435..3455
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     CDS             3456..6977
                     /codon_start=1
                     /label=dPguCas13b
                     /translation="TEQSERPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAY
                     SKADITNDQDVLSFKALWKNFDNDLERKSRLRSLILKHFSFLEGAAYGKKLFESKSSGN
                     KSSKNKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSAYRHSGSSELPLFD
                     GNMLQRLYNVFDVSVQRVKIDHEHNDEVDPHYHFNHLVRKGKKDRYGHNDNPSFKHHFV
                     DGEGMVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTETYQQMTNEVFCRSRISLPKL
                     KLESLRMDDWMLLDMLNELVRCPKPLYDRLREDDRACFRVPVDILPDEDDTDGGGEDPF
                     KNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKMIGEQPEDRHLTRNL
                     YGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYISQTSPHYHIEKGKIGLRFMPEGQ
                     HLWPSPEVGTTRTGRSKYAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAERVQ
                     GRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEE
                     KIRKKLQEMMADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAK
                     DASGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWE
                     SHTNILSFYRSYLRARKAFLERIGRSDRVENRPFLLLKEPKTDRQTLVAGWKGEFHLPR
                     GIFTEAVRDCLIEMGHDEVASYKEVGFMAKAVPLYFERACEDRVQPFYDSPFNVGNSLK
                     PKKGRFLSKEERAEEWERGKERFRDLEAWSYSAARRIEDAFAGIEYASPGNKKKIEQLL
                     RDLSLWEAFESKLKVRADRINLAKLKKEILEAQEHPYHDFKSWQKFERELRLVKNQDII
                     TWMMCRDLMEENKVEGLDTGTLYLKDIRPNVQEQGSLNVLNRVKPMRLPVVVYRADSRG
                     HVHKEEAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLR
                     VEYELAKYQTARVCVFELTLRLEESLLTRYPHLPDESFREMLESWSDPLLAKWPELHGK
                     VRLLIAVRNAFSANQYPMYDEAVFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKE
                     TVERIIQA"
     CDS             7005..7721
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7749..7796
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7815..7880
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7887..8454
                     /label=IRES
     CDS             8474..9073
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9092..9680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9563..9574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9755..9988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10193..10297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10298..11158
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11329..11917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12328..12349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12364..12394
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12402..12418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12426..12442
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag catgcccaag aaaaagcgca aggtaacaga gcagagcgag aggccctaca
     3481 acggcaccta ctacaccctg gaagataagc acttctgggc cgcctttctg aacctggcca
     3541 gacacaacgc ctacatcacc ctgacacaca tcgaccggca gctggcctac agcaaggccg
     3601 acatcaccaa cgaccaggac gtgctgagct tcaaggccct gtggaagaac ttcgacaacg
     3661 acctggaaag aaagagccgg ctgcggagcc tgatcctgaa gcacttcagc tttctggaag
     3721 gcgccgctta cggcaagaag ctgttcgagt ctaagagcag cggcaacaag agcagcaaga
     3781 acaaagagct gaccaagaaa gagaaagagg aactgcaggc caacgctctg agcctggaca
     3841 acctgaagtc tatcctgttc gacttcctgc agaagctgaa ggacttccgg aactactaca
     3901 gcgcctaccg gcacagcgga agcagcgagc tgcctctgtt cgacggcaac atgctgcagc
     3961 ggctgtacaa cgtgttcgac gtgtccgtgc agcgcgtgaa gatcgaccac gagcacaacg
     4021 atgaggtgga ccctcactac cacttcaacc acctcgtgcg gaagggcaag aaggacagat
     4081 acggccacaa cgacaacccc agcttcaagc accacttcgt ggacggcgaa ggcatggtta
     4141 cagaagccgg cctgctgttc ttcgtgtccc tgttcctgga aaagcgggac gccatctgga
     4201 tgcagaagaa gatccggggc ttcaaaggcg gcaccgagac ataccagcag atgaccaacg
     4261 aggtgttctg ccggtccaga atcagcctgc ctaagctgaa gctggaaagc ctgcggatgg
     4321 acgactggat gctgctggac atgctgaacg aactcgtgcg gtgccccaag cctctgtacg
     4381 acagactgag agaggacgac cgggcctgct ttagagtgcc cgtggatatc ctgcctgacg
     4441 aggacgatac agatggcggc ggagaggacc ccttcaagaa taccctcgtc cggcaccagg
     4501 acagattccc ttacttcgcc ctgcggtact tcgacctgaa gaaggtgttc accagcctgc
     4561 ggttccacat cgatctgggc acctaccact ttgccatcta caagaagatg atcggcgagc
     4621 agcccgagga cagacacctg accagaaacc tgtacggctt cggccggatc caggacttcg
     4681 ccgaagaaca cagacccgag gaatggaagc ggctcgtcag agatctggac tacttcgaga
     4741 caggcgacaa gccctacatc agccagacaa gcccacacta ccacatcgag aagggaaaga
     4801 tcggcctgag attcatgccc gagggccagc atctttggcc ctctccagaa gtgggcacca
     4861 ccagaaccgg cagatctaag tacgcccagg acaagagact gaccgccgag gcctttctgt
     4921 ctgtgcacga gctgatgcct atgatgttct actacttcct gctgcgcgag aagtacagcg
     4981 aggaagtgtc tgccgagaga gtgcagggca gaatcaagcg cgtgatcgag gatgtgtacg
     5041 ccgtgtacga tgcctttgcc agggacgaga tcaacacccg ggatgagctg gatgcctgcc
     5101 tggccgataa gggcatcaga agaggccatc tgcctcgcca gatgatcgcc atcctgagcc
     5161 aagagcacaa ggacatggaa gagaagattc ggaagaaact gcaagagatg atggccgaca
     5221 ccgaccaccg gctggatatg ctggatagac agaccgaccg gaagatcaga atcggccgga
     5281 agaatgccgg actgcctaag agcggagtga tcgccgattg gctcgtgcgg gacatgatga
     5341 gatttcagcc cgtggccaag gacgccagcg gaaagcctct gaacaactcc aaggccaaca
     5401 gcaccgagta ccggatgctg cagagagccc tggctctgtt tggaggcgag aaagagaggc
     5461 tgaccccata cttccggcag atgaatctga ccggcggaaa caaccctcat ccttttctgc
     5521 acgaaactcg ctgggagagc cacaccaaca tcctgtcctt ttaccggtcc tacctgagag
     5581 cccgcaaggc cttcctcgaa aggatcggca gatccgacag agtggaaaac cggccatttc
     5641 tgctgctgaa agagcccaag accgacagac agacactggt ggctggatgg aagggcgagt
     5701 tccatctgcc aagaggcatc ttcacagagg ccgtgcggga ttgcctgatc gagatgggac
     5761 acgatgaagt ggccagctac aaagaagtgg gattcatggc caaggccgtg ccactgtact
     5821 ttgagagagc ctgcgaggac agggtgcagc ccttttacga cagccccttc aacgtgggca
     5881 acagcctgaa gcctaagaag ggcagattcc tgagcaaaga ggaacgggcc gaagagtggg
     5941 agcggggcaa agagagattc cgggatctcg aagcctggtc ctatagcgcc gccagaagaa
     6001 tcgaggacgc ctttgccggc atcgagtatg ctagccccgg aaacaagaag aaaatcgagc
     6061 agctcctgcg ggacctgagc ctgtgggaag cctttgagag caagctgaaa gtgcgggccg
     6121 accggatcaa tctggccaag ctgaagaaag aaatcctgga agcccaagaa cacccctacc
     6181 acgacttcaa gagctggcag aagttcgagc gcgagctgcg gctggtcaag aaccaggata
     6241 tcatcacctg gatgatgtgc cgcgacctga tggaagaaaa caaggtggaa ggcctggaca
     6301 ccggcacact gtacctgaag gacatcagac ccaacgtgca agagcagggc agcctgaacg
     6361 tgctgaacag agtgaagccc atgagactgc ccgtggtggt gtacagagcc gatagcagag
     6421 gccacgtgca caaagaagaa gcccctctgg ccaccgtgta catcgaggaa agagacacca
     6481 agctgctgaa gcagggcaac ttcaagtcct tcgtgaagga caggcggctg aacggcctgt
     6541 tctccttcgt tgatactggc ggactggcca tggaacagta ccccatctcc aagctgagag
     6601 tggaatacga gctggccaag taccagaccg ccagagtgtg tgtgttcgag ctgaccctga
     6661 gactggaaga gtccctgctg acccggtatc ctcatctgcc cgacgagagc ttcagagaga
     6721 tgctggaatc ttggagcgac cctctgctgg ccaaatggcc tgaactgcac ggaaaagtgc
     6781 ggctgctgat cgccgtgcgg aatgccttta gcgccaatca gtaccctatg tacgacgagg
     6841 ccgtgttcag ctccatccgg aagtacgatc ctagcagccc cgacgccatc gaagagagaa
     6901 tgggcctgaa tatcgcccac cgcctgtctg aggaagtgaa gcaggccaaa gaaaccgtgg
     6961 aacggatcat tcaggccagt ctagaggcgc taccggtcgc caccatggtg agcaagggcg
     7021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     7081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     7141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7201 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     7441 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtcgaattct aagccaggat
     7741 tccatatgaa gcgacctgcc gccacaaaga aggctggaca ggctaagaag aagaaacaac
     7801 gcgtgcagct aagcgactac aaagaccatg acggtgatta taaagatcat gacatcgatt
     7861 acaaggatga cgatgacaag taaggatccc tccccccccc ctaacgttac tggccgaagc
     7921 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7981 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     8041 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     8101 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     8161 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     8221 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     8281 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     8341 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     8401 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc
     8461 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8521 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8581 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8641 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8701 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8761 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8821 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8881 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8941 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9001 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9061 gcccggtgcc tgacgcccat cgatagatcc taatcaacct ctggattaca aaatttgtga
     9121 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     9181 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     9241 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     9301 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9361 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9421 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9481 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9541 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9601 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9661 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     9721 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9781 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9961 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    10021 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
    10081 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    10141 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
    10201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10501 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
    10561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    12001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    12061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    12121 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
    12181 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    12241 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
    12301 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    12361 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    12421 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
    12481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    12541 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    12601 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    12661 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
