LOCUS       Exported               13179 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3435..3455
                     /codon_start=1
                     /label=nls
                     /translation="PKKKRKV"
     CDS             3456..6722
                     /codon_start=1
                     /label=dPspcas13b
                     /translation="NIPALVENQKKYFGTYSVMAMLNAQTVLDHIQKVADIEGEQNENN
                     ENLWFHPVMSHLYNAKNGYDKQPEKTMFIIERLQSYFPFLKIMAENQREYSNGKYKQNR
                     VEVNSNDIFEVLKRAFGVLKMYRDLTNAYKTYEEKLNDGCEFLTSTEQPLSGMINNYYT
                     VALRNMNERYGYKTEDLAFIQDKRFKFVKDAYGKKKSQVNTGFFLSLQDYNGDTQKKLH
                     LSGVGIALLICLFLDKQYINIFLSRLPIFSSYNAQSEERRIIIRSFGINSIKLPKDRIH
                     SEKSNKSVAMDMLNEVKRCPDELFTTLSAEKQSRFRIISDDHNEVLMKRSSDRFVPLLL
                     QYIDYGKLFDHIRFHVNMGKLRYLLKADKTCIDGQTRVRVIEQPLNGFGRLEEAETMRK
                     QENGTFGNSGIRIRDFENMKRDDANPANYPYIVDTYTHYILENNKVEMFINDKEDSAPL
                     LPVIEDDRYVVKTIPSCRMSTLEIPAMAFHMFLFGSKKTEKLIVDVHNRYKRLFQAMQK
                     EEVTAENIASFGIAESDLPQKILDLISGNAHGKDVDAFIRLTVDDMLTDTERRIKRFKD
                     DRKSIRSADNKMGKRGFKQISTGKLADFLAKDIVLFQPSVNDGENKITGLNYRIMQSAI
                     AVYDSGDDYEAKQQFKLMFEKARLIGKGTTEPHPFLYKVFARSIPANAVEFYERYLIER
                     KFYLTGLSNEIKKGNRVDVPFIRRDQNKWKTPAMKTLGRIYSEDLPVELPRQMFDNEIK
                     SHLKSLPQMEGIDFNNANVTYLIAEYMKRVLDDDFQTFYQWNRNYRYMDMLKGEYDRKG
                     SLQHCFTSVEEREGLWKERASRTERYRKQASNKIRSNRQMRNASSEEIETILDKRLSNS
                     RNEYQKSEKVIRRYRVQDALLFLLAKKTLTELADFDGERFKLKEIMPDAEKGILSEIMP
                     MSFTFEKGGKKYTITSEGMKLKNYGDFFVLASDKRIGNLLELVGSDIVSKEDIMEEFNK
                     YDQCRPEISSIVFNLEKWAFDTYPELSARVDREEKVDFKSILKILLNNKNINKEQSDIL
                     RKIRNAFDANNYPDKGVVEIKALPEIAMSIKKAFGEYAIMK"
     regulatory      6722
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6759..7466
                     /codon_start=1
                     /label=mNeongreen
                     /note="modified_by=User"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     CDS             7491..8198
                     /codon_start=1
                     /label=mNeongreen
                     /note="modified_by=User"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    8213
                     /label=MCS
                     /note="multiple cloning site"
     CDS             8226..8273
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8292..8357
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8364..8931
                     /label=IRES
     CDS             8951..9550
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9569..10157
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10040..10051)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10232..10465
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10670..10774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10775..11635
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11806..12394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12805..12826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12841..12871
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12879..12895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12903..12919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag catgcccaaa aagaagagga aagtgaacat ccccgctctg gtggaaaacc
     3481 agaagaagta ctttggcacc tacagcgtga tggccatgct gaacgctcag accgtgctgg
     3541 accacatcca gaaggtggcc gatattgagg gcgagcagaa cgagaacaac gagaatctgt
     3601 ggtttcaccc cgtgatgagc cacctgtaca acgccaagaa cggctacgac aagcagcccg
     3661 agaaaaccat gttcatcatc gagcggctgc agagctactt cccattcctg aagatcatgg
     3721 ccgagaacca gagagagtac agcaacggca agtacaagca gaaccgcgtg gaagtgaaca
     3781 gcaacgacat cttcgaggtg ctgaagcgcg ccttcggcgt gctgaagatg tacagggacc
     3841 tgaccaacgc ctacaagacc tacgaggaaa agctgaacga cggctgcgag ttcctgacca
     3901 gcacagagca acctctgagc ggcatgatca acaactacta cacagtggcc ctgcggaaca
     3961 tgaacgagag atacggctac aagacagagg acctggcctt catccaggac aagcggttca
     4021 agttcgtgaa ggacgcctac ggcaagaaaa agtcccaagt gaataccgga ttcttcctga
     4081 gcctgcagga ctacaacggc gacacacaga agaagctgca cctgagcgga gtgggaatcg
     4141 ccctgctgat ctgcctgttc ctggacaagc agtacatcaa catctttctg agcaggctgc
     4201 ccatcttctc cagctacaat gcccagagcg aggaacggcg gatcatcatc agatccttcg
     4261 gcatcaacag catcaagctg cccaaggacc ggatccacag cgagaagtcc aacaagagcg
     4321 tggccatgga tatgctcaac gaagtgaagc ggtgccccga cgagctgttc acaacactgt
     4381 ctgccgagaa gcagtcccgg ttcagaatca tcagcgacga ccacaatgaa gtgctgatga
     4441 agcggagcag cgacagattc gtgcctctgc tgctgcagta tatcgattac ggcaagctgt
     4501 tcgaccacat caggttccac gtgaacatgg gcaagctgag atacctgctg aaggccgaca
     4561 agacctgcat cgacggccag accagagtca gagtgatcga gcagcccctg aacggcttcg
     4621 gcagactgga agaggccgag acaatgcgga agcaagagaa cggcaccttc ggcaacagcg
     4681 gcatccggat cagagacttc gagaacatga agcgggacga cgccaatcct gccaactatc
     4741 cctacatcgt ggacacctac acacactaca tcctggaaaa caacaaggtc gagatgttta
     4801 tcaacgacaa agaggacagc gccccactgc tgcccgtgat cgaggatgat agatacgtgg
     4861 tcaagacaat ccccagctgc cggatgagca ccctggaaat tccagccatg gccttccaca
     4921 tgtttctgtt cggcagcaag aaaaccgaga agctgatcgt ggacgtgcac aaccggtaca
     4981 agagactgtt ccaggccatg cagaaagaag aagtgaccgc cgagaatatc gccagcttcg
     5041 gaatcgccga gagcgacctg cctcagaaga tcctggatct gatcagcggc aatgcccacg
     5101 gcaaggatgt ggacgccttc atcagactga ccgtggacga catgctgacc gacaccgagc
     5161 ggagaatcaa gagattcaag gacgaccgga agtccattcg gagcgccgac aacaagatgg
     5221 gaaagagagg cttcaagcag atctccacag gcaagctggc cgacttcctg gccaaggaca
     5281 tcgtgctgtt tcagcccagc gtgaacgatg gcgagaacaa gatcaccggc ctgaactacc
     5341 ggatcatgca gagcgccatt gccgtgtacg atagcggcga cgattacgag gccaagcagc
     5401 agttcaagct gatgttcgag aaggcccggc tgatcggcaa gggcacaaca gagcctcatc
     5461 catttctgta caaggtgttc gcccgcagca tccccgccaa tgccgtcgag ttctacgagc
     5521 gctacctgat cgagcggaag ttctacctga ccggcctgtc caacgagatc aagaaaggca
     5581 acagagtgga tgtgcccttc atccggcggg accagaacaa gtggaaaaca cccgccatga
     5641 agaccctggg cagaatctac agcgaggatc tgcccgtgga actgcccaga cagatgttcg
     5701 acaatgagat caagtcccac ctgaagtccc tgccacagat ggaaggcatc gacttcaaca
     5761 atgccaacgt gacctatctg atcgccgagt acatgaagag agtgctggac gacgacttcc
     5821 agaccttcta ccagtggaac cgcaactacc ggtacatgga catgcttaag ggcgagtacg
     5881 acagaaaggg ctccctgcag cactgcttca ccagcgtgga agagagagaa ggcctctgga
     5941 aagagcgggc ctccagaaca gagcggtaca gaaagcaggc cagcaacaag atccgcagca
     6001 accggcagat gagaaacgcc agcagcgaag agatcgagac aatcctggat aagcggctga
     6061 gcaacagccg gaacgagtac cagaaaagcg agaaagtgat ccggcgctac agagtgcagg
     6121 atgccctgct gtttctgctg gccaaaaaga ccctgaccga actggccgat ttcgacggcg
     6181 agaggttcaa actgaaagaa atcatgcccg acgccgagaa gggaatcctg agcgagatca
     6241 tgcccatgag cttcaccttc gagaaaggcg gcaagaagta caccatcacc agcgagggca
     6301 tgaagctgaa gaactacggc gacttctttg tgctggctag cgacaagagg atcggcaacc
     6361 tgctggaact cgtgggcagc gacatcgtgt ccaaagagga tatcatggaa gagttcaaca
     6421 aatacgacca gtgcaggccc gagatcagct ccatcgtgtt caacctggaa aagtgggcct
     6481 tcgacacata ccccgagctg tctgccagag tggaccggga agagaaggtg gacttcaaga
     6541 gcatcctgaa aatcctgctg aacaacaaga acatcaacaa agagcagagc gacatcctgc
     6601 ggaagatccg gaacgccttc gatgccaaca attaccccga caaaggcgtg gtggaaatca
     6661 aggccctgcc tgagatcgcc atgagcatca agaaggcctt tggggagtac gccatcatga
     6721 aggagggagc tctagagggg actagtggag gtggatccat ggtgtccaag ggcgaagagg
     6781 acaacatggc cagcctgcct gccacccacg agctgcacat cttcggcagc atcaacggcg
     6841 tggacttcga catggtggga cagggcaccg gcaaccccaa cgacggctac gaggaactga
     6901 acctgaagtc cacaaagggc gacctgcagt tcagcccctg gattctggtg ccccacatcg
     6961 gctacggctt ccaccagtac ctgccctacc ccgacggcat gagccctttt caggccgcca
     7021 tggtggatgg cagcggctac caggtgcacc ggaccatgca gtttgaggac ggcgccagcc
     7081 tgaccgtgaa ctaccggtac acatacgagg gcagccacat caagggcgag gcccaagtga
     7141 agggcacagg ctttccagcc gacggccccg tgatgaccaa tagcctgaca gccgccgact
     7201 ggtgcagaag caagaaaacc taccccaatg acaagaccat catcagcacc ttcaagtggt
     7261 cctacaccac cggcaatggc aagcggtaca gaagcaccgc ccggaccacc tacaccttcg
     7321 ccaaacccat ggccgccaac tacctgaaga accagcctat gtacgtgttc cgcaagaccg
     7381 agctgaagca ctccaagaca gaactgaact tcaaagagtg gcagaaagcc ttcaccgacg
     7441 tgatgggcat ggacgagctg tacaagtcga attctgcagt cgacggtacc atggtgtcca
     7501 agggcgaaga ggacaacatg gccagcctgc ctgccaccca cgagctgcac atcttcggca
     7561 gcatcaacgg cgtggacttc gacatggtgg gacagggcac cggcaacccc aacgacggct
     7621 acgaggaact gaacctgaag tccacaaagg gcgacctgca gttcagcccc tggattctgg
     7681 tgccccacat cggctacggc ttccaccagt acctgcccta ccccgacggc atgagccctt
     7741 ttcaggccgc catggtggat ggcagcggct accaggtgca ccggaccatg cagtttgagg
     7801 acggcgccag cctgaccgtg aactaccggt acacatacga gggcagccac atcaagggcg
     7861 aggcccaagt gaagggcaca ggctttccag ccgacggccc cgtgatgacc aatagcctga
     7921 cagccgccga ctggtgcaga agcaagaaaa cctaccccaa tgacaagacc atcatcagca
     7981 ccttcaagtg gtcctacacc accggcaatg gcaagcggta cagaagcacc gcccggacca
     8041 cctacacctt cgccaaaccc atggccgcca actacctgaa gaaccagcct atgtacgtgt
     8101 tccgcaagac cgagctgaag cactccaaga cagaactgaa cttcaaagag tggcagaaag
     8161 ccttcaccga cgtgatgggc atggacgagc tgtacaagtc cggactcaga tcttgcctac
     8221 atatgaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaacaacgcg
     8281 tgcagctaag cgactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca
     8341 aggatgacga tgacaagtaa ggatccctcc ccccccccta acgttactgg ccgaagccgc
     8401 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     8461 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     8521 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     8581 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     8641 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     8701 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     8761 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     8821 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     8881 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata aggatccacc
     8941 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9001 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9061 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9121 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     9181 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9241 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9301 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9361 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9421 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9481 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9541 cggtgcctga cgcccatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     9601 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     9661 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9721 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9781 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9841 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9901 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9961 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    10021 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    10081 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    10141 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
    10201 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
    10261 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
    10321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10441 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
    10501 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
    10561 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    10621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    11041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    11521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    11581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    11641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    12541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    12601 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
    12661 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    12721 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
    12781 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12841 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12901 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
    12961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    13021 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    13081 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    13141 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
