LOCUS Exported 13128 bp ds-DNA circular SYN 09-AUG-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13128) AUTHORS ylz TITLE Direct Submission JOURNAL Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10 https://www.snapgene.com FEATURES Location/Qualifiers source 1..13128 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2224..3405 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3396 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" CDS 3435..3453 /codon_start=1 /label=NLS /translation="PKKKRK" CDS 3456..6722 /codon_start=1 /label=dPspcas13b /translation="NIPALVENQKKYFGTYSVMAMLNAQTVLDHIQKVADIEGEQNENN ENLWFHPVMSHLYNAKNGYDKQPEKTMFIIERLQSYFPFLKIMAENQREYSNGKYKQNR VEVNSNDIFEVLKRAFGVLKMYRDLTNAYKTYEEKLNDGCEFLTSTEQPLSGMINNYYT VALRNMNERYGYKTEDLAFIQDKRFKFVKDAYGKKKSQVNTGFFLSLQDYNGDTQKKLH LSGVGIALLICLFLDKQYINIFLSRLPIFSSYNAQSEERRIIIRSFGINSIKLPKDRIH SEKSNKSVAMDMLNEVKRCPDELFTTLSAEKQSRFRIISDDHNEVLMKRSSDRFVPLLL QYIDYGKLFDHIRFHVNMGKLRYLLKADKTCIDGQTRVRVIEQPLNGFGRLEEAETMRK QENGTFGNSGIRIRDFENMKRDDANPANYPYIVDTYTHYILENNKVEMFINDKEDSAPL LPVIEDDRYVVKTIPSCRMSTLEIPAMAFHMFLFGSKKTEKLIVDVHNRYKRLFQAMQK EEVTAENIASFGIAESDLPQKILDLISGNAHGKDVDAFIRLTVDDMLTDTERRIKRFKD DRKSIRSADNKMGKRGFKQISTGKLADFLAKDIVLFQPSVNDGENKITGLNYRIMQSAI AVYDSGDDYEAKQQFKLMFEKARLIGKGTTEPHPFLYKVFARSIPANAVEFYERYLIER KFYLTGLSNEIKKGNRVDVPFIRRDQNKWKTPAMKTLGRIYSEDLPVELPRQMFDNEIK SHLKSLPQMEGIDFNNANVTYLIAEYMKRVLDDDFQTFYQWNRNYRYMDMLKGEYDRKG SLQHCFTSVEEREGLWKERASRTERYRKQASNKIRSNRQMRNASSEEIETILDKRLSNS RNEYQKSEKVIRRYRVQDALLFLLAKKTLTELADFDGERFKLKEIMPDAEKGILSEIMP MSFTFEKGGKKYTITSEGMKLKNYGDFFVLASDKRIGNLLELVGSDIVSKEDIMEEFNK YDQCRPEISSIVFNLEKWAFDTYPELSARVDREEKVDFKSILKILLNNKNINKEQSDIL RKIRNAFDANNYPDKGVVEIKALPEIAMSIKKAFGEYAIMK" regulatory 6722 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6722 /codon_start=1 /product="enhanced CFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mECFP /note="mammalian codon-optimized" /translation="" CDS 6759..7466 /label=mNeongreen /note="modified_by=User" CDS 7491..8198 /label=mNeongreen /note="modified_by=User" misc_feature 8213 /label=MCS /note="multiple cloning site" CDS 8241..8306 /codon_start=1 /product="three tandem FLAG epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 8313..8880 /label=IRES CDS 8900..9499 /codon_start=1 /label=PuroR /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 9518..10106 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(9989..10000) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 10181..10414 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter 10619..10723 /gene="bla" /label=AmpR promoter CDS 10724..11584 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 11755..12343 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 12754..12775 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12790..12820 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12828..12844 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12852..12868 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt 2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag 2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc 2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt 2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt 2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc 2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg 2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg 3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa 3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc 3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac 3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat 3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg 3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct 3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt 3421 ctgcagctag catgcccaaa aagaagagga aagtgaacat ccccgctctg gtggaaaacc 3481 agaagaagta ctttggcacc tacagcgtga tggccatgct gaacgctcag accgtgctgg 3541 accacatcca gaaggtggcc gatattgagg gcgagcagaa cgagaacaac gagaatctgt 3601 ggtttcaccc cgtgatgagc cacctgtaca acgccaagaa cggctacgac aagcagcccg 3661 agaaaaccat gttcatcatc gagcggctgc agagctactt cccattcctg aagatcatgg 3721 ccgagaacca gagagagtac agcaacggca agtacaagca gaaccgcgtg gaagtgaaca 3781 gcaacgacat cttcgaggtg ctgaagcgcg ccttcggcgt gctgaagatg tacagggacc 3841 tgaccaacgc ctacaagacc tacgaggaaa agctgaacga cggctgcgag ttcctgacca 3901 gcacagagca acctctgagc ggcatgatca acaactacta cacagtggcc ctgcggaaca 3961 tgaacgagag atacggctac aagacagagg acctggcctt catccaggac aagcggttca 4021 agttcgtgaa ggacgcctac ggcaagaaaa agtcccaagt gaataccgga ttcttcctga 4081 gcctgcagga ctacaacggc gacacacaga agaagctgca cctgagcgga gtgggaatcg 4141 ccctgctgat ctgcctgttc ctggacaagc agtacatcaa catctttctg agcaggctgc 4201 ccatcttctc cagctacaat gcccagagcg aggaacggcg gatcatcatc agatccttcg 4261 gcatcaacag catcaagctg cccaaggacc ggatccacag cgagaagtcc aacaagagcg 4321 tggccatgga tatgctcaac gaagtgaagc ggtgccccga cgagctgttc acaacactgt 4381 ctgccgagaa gcagtcccgg ttcagaatca tcagcgacga ccacaatgaa gtgctgatga 4441 agcggagcag cgacagattc gtgcctctgc tgctgcagta tatcgattac ggcaagctgt 4501 tcgaccacat caggttccac gtgaacatgg gcaagctgag atacctgctg aaggccgaca 4561 agacctgcat cgacggccag accagagtca gagtgatcga gcagcccctg aacggcttcg 4621 gcagactgga agaggccgag acaatgcgga agcaagagaa cggcaccttc ggcaacagcg 4681 gcatccggat cagagacttc gagaacatga agcgggacga cgccaatcct gccaactatc 4741 cctacatcgt ggacacctac acacactaca tcctggaaaa caacaaggtc gagatgttta 4801 tcaacgacaa agaggacagc gccccactgc tgcccgtgat cgaggatgat agatacgtgg 4861 tcaagacaat ccccagctgc cggatgagca ccctggaaat tccagccatg gccttccaca 4921 tgtttctgtt cggcagcaag aaaaccgaga agctgatcgt ggacgtgcac aaccggtaca 4981 agagactgtt ccaggccatg cagaaagaag aagtgaccgc cgagaatatc gccagcttcg 5041 gaatcgccga gagcgacctg cctcagaaga tcctggatct gatcagcggc aatgcccacg 5101 gcaaggatgt ggacgccttc atcagactga ccgtggacga catgctgacc gacaccgagc 5161 ggagaatcaa gagattcaag gacgaccgga agtccattcg gagcgccgac aacaagatgg 5221 gaaagagagg cttcaagcag atctccacag gcaagctggc cgacttcctg gccaaggaca 5281 tcgtgctgtt tcagcccagc gtgaacgatg gcgagaacaa gatcaccggc ctgaactacc 5341 ggatcatgca gagcgccatt gccgtgtacg atagcggcga cgattacgag gccaagcagc 5401 agttcaagct gatgttcgag aaggcccggc tgatcggcaa gggcacaaca gagcctcatc 5461 catttctgta caaggtgttc gcccgcagca tccccgccaa tgccgtcgag ttctacgagc 5521 gctacctgat cgagcggaag ttctacctga ccggcctgtc caacgagatc aagaaaggca 5581 acagagtgga tgtgcccttc atccggcggg accagaacaa gtggaaaaca cccgccatga 5641 agaccctggg cagaatctac agcgaggatc tgcccgtgga actgcccaga cagatgttcg 5701 acaatgagat caagtcccac ctgaagtccc tgccacagat ggaaggcatc gacttcaaca 5761 atgccaacgt gacctatctg atcgccgagt acatgaagag agtgctggac gacgacttcc 5821 agaccttcta ccagtggaac cgcaactacc ggtacatgga catgcttaag ggcgagtacg 5881 acagaaaggg ctccctgcag cactgcttca ccagcgtgga agagagagaa ggcctctgga 5941 aagagcgggc ctccagaaca gagcggtaca gaaagcaggc cagcaacaag atccgcagca 6001 accggcagat gagaaacgcc agcagcgaag agatcgagac aatcctggat aagcggctga 6061 gcaacagccg gaacgagtac cagaaaagcg agaaagtgat ccggcgctac agagtgcagg 6121 atgccctgct gtttctgctg gccaaaaaga ccctgaccga actggccgat ttcgacggcg 6181 agaggttcaa actgaaagaa atcatgcccg acgccgagaa gggaatcctg agcgagatca 6241 tgcccatgag cttcaccttc gagaaaggcg gcaagaagta caccatcacc agcgagggca 6301 tgaagctgaa gaactacggc gacttctttg tgctggctag cgacaagagg atcggcaacc 6361 tgctggaact cgtgggcagc gacatcgtgt ccaaagagga tatcatggaa gagttcaaca 6421 aatacgacca gtgcaggccc gagatcagct ccatcgtgtt caacctggaa aagtgggcct 6481 tcgacacata ccccgagctg tctgccagag tggaccggga agagaaggtg gacttcaaga 6541 gcatcctgaa aatcctgctg aacaacaaga acatcaacaa agagcagagc gacatcctgc 6601 ggaagatccg gaacgccttc gatgccaaca attaccccga caaaggcgtg gtggaaatca 6661 aggccctgcc tgagatcgcc atgagcatca agaaggcctt tggggagtac gccatcatga 6721 aggagggagc tctagagggg actagtggag gtggatccat ggtgtccaag ggcgaagagg 6781 acaacatggc cagcctgcct gccacccacg agctgcacat cttcggcagc atcaacggcg 6841 tggacttcga catggtggga cagggcaccg gcaaccccaa cgacggctac gaggaactga 6901 acctgaagtc cacaaagggc gacctgcagt tcagcccctg gattctggtg ccccacatcg 6961 gctacggctt ccaccagtac ctgccctacc ccgacggcat gagccctttt caggccgcca 7021 tggtggatgg cagcggctac caggtgcacc ggaccatgca gtttgaggac ggcgccagcc 7081 tgaccgtgaa ctaccggtac acatacgagg gcagccacat caagggcgag gcccaagtga 7141 agggcacagg ctttccagcc gacggccccg tgatgaccaa tagcctgaca gccgccgact 7201 ggtgcagaag caagaaaacc taccccaatg acaagaccat catcagcacc ttcaagtggt 7261 cctacaccac cggcaatggc aagcggtaca gaagcaccgc ccggaccacc tacaccttcg 7321 ccaaacccat ggccgccaac tacctgaaga accagcctat gtacgtgttc cgcaagaccg 7381 agctgaagca ctccaagaca gaactgaact tcaaagagtg gcagaaagcc ttcaccgacg 7441 tgatgggcat ggacgagctg tacaagtcga attctgcagt cgacggtacc atggtgtcca 7501 agggcgaaga ggacaacatg gccagcctgc ctgccaccca cgagctgcac atcttcggca 7561 gcatcaacgg cgtggacttc gacatggtgg gacagggcac cggcaacccc aacgacggct 7621 acgaggaact gaacctgaag tccacaaagg gcgacctgca gttcagcccc tggattctgg 7681 tgccccacat cggctacggc ttccaccagt acctgcccta ccccgacggc atgagccctt 7741 ttcaggccgc catggtggat ggcagcggct accaggtgca ccggaccatg cagtttgagg 7801 acggcgccag cctgaccgtg aactaccggt acacatacga gggcagccac atcaagggcg 7861 aggcccaagt gaagggcaca ggctttccag ccgacggccc cgtgatgacc aatagcctga 7921 cagccgccga ctggtgcaga agcaagaaaa cctaccccaa tgacaagacc atcatcagca 7981 ccttcaagtg gtcctacacc accggcaatg gcaagcggta cagaagcacc gcccggacca 8041 cctacacctt cgccaaaccc atggccgcca actacctgaa gaaccagcct atgtacgtgt 8101 tccgcaagac cgagctgaag cactccaaga cagaactgaa cttcaaagag tggcagaaag 8161 ccttcaccga cgtgatgggc atggacgagc tgtacaagtc cggactcaga tcttgcctag 8221 agcaacgcgt gcagctaagc gactacaaag accatgacgg tgattataaa gatcatgaca 8281 tcgattacaa ggatgacgat gacaagtaag gatccctccc ccccccctaa cgttactggc 8341 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 8401 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 8461 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 8521 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 8581 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 8641 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 8701 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 8761 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 8821 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 8881 ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 8941 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 9001 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 9061 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 9121 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 9181 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 9241 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 9301 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 9361 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 9421 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 9481 ccgcaagccc ggtgcctgac gcccatcgat agatcctaat caacctctgg attacaaaat 9541 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 9601 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 9661 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 9721 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 9781 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 9841 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 9901 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 9961 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 10021 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 10081 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca 10141 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 10201 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 10261 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 10321 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 10381 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 10441 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag 10501 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt 10561 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 10621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 10681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 10741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 10801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 10861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 10921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 10981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 11041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 11101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 11161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 11221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 11281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 11341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 11401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 11461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 11521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 11581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 11641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 11701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 11761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 11821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 11881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 11941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 12001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 12061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 12121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 12181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 12241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 12301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 12361 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 12421 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 12481 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 12541 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt 12601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 12661 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc 12721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 12781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 12841 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt 12901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 12961 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac 13021 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc 13081 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt //