LOCUS       Exported               13079 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   young
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3435..3455
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     CDS             3456..6977
                     /codon_start=1
                     /label=dPguCas13b
                     /translation="TEQSERPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAY
                     SKADITNDQDVLSFKALWKNFDNDLERKSRLRSLILKHFSFLEGAAYGKKLFESKSSGN
                     KSSKNKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSAYRHSGSSELPLFD
                     GNMLQRLYNVFDVSVQRVKIDHEHNDEVDPHYHFNHLVRKGKKDRYGHNDNPSFKHHFV
                     DGEGMVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTETYQQMTNEVFCRSRISLPKL
                     KLESLRMDDWMLLDMLNELVRCPKPLYDRLREDDRACFRVPVDILPDEDDTDGGGEDPF
                     KNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKMIGEQPEDRHLTRNL
                     YGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYISQTSPHYHIEKGKIGLRFMPEGQ
                     HLWPSPEVGTTRTGRSKYAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAERVQ
                     GRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEE
                     KIRKKLQEMMADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAK
                     DASGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWE
                     SHTNILSFYRSYLRARKAFLERIGRSDRVENRPFLLLKEPKTDRQTLVAGWKGEFHLPR
                     GIFTEAVRDCLIEMGHDEVASYKEVGFMAKAVPLYFERACEDRVQPFYDSPFNVGNSLK
                     PKKGRFLSKEERAEEWERGKERFRDLEAWSYSAARRIEDAFAGIEYASPGNKKKIEQLL
                     RDLSLWEAFESKLKVRADRINLAKLKKEILEAQEHPYHDFKSWQKFERELRLVKNQDII
                     TWMMCRDLMEENKVEGLDTGTLYLKDIRPNVQEQGSLNVLNRVKPMRLPVVVYRADSRG
                     HVHKEEAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLR
                     VEYELAKYQTARVCVFELTLRLEESLLTRYPHLPDESFREMLESWSDPLLAKWPELHGK
                     VRLLIAVRNAFSANQYPMYDEAVFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKE
                     TVERIIQA"
     CDS             7005..7715
                     /label=mRuby3
                     /note="created_by=User
                     modified_by=User"
     misc_feature    7128..7130
                     /label=T38V
                     /note="created_by=User"
     CDS             7731..8438
                     /label=mRuby3
                     /note="created_by=User
                     modified_by=User"
     misc_feature    7851..7853
                     /label=T38V
                     /note="created_by=User"
     misc_feature    8439
                     /label=MCS
                     /note="multiple cloning site"
     CDS             8445..9152
                     /label=mRuby3
                     /note="created_by=User
                     modified_by=User"
     misc_feature    8565..8567
                     /label=T38V
                     /note="created_by=User"
     misc_feature    9167..9180
                     /label=MCS
                     /note="multiple cloning site"
     CDS             9186..9233
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    9469..10057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9940..9951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10132..10365
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10570..10674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10675..11535
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11706..12294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12705..12726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12741..12771
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12779..12795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12803..12819
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag catgcccaag aaaaagcgca aggtaacaga gcagagcgag aggccctaca
     3481 acggcaccta ctacaccctg gaagataagc acttctgggc cgcctttctg aacctggcca
     3541 gacacaacgc ctacatcacc ctgacacaca tcgaccggca gctggcctac agcaaggccg
     3601 acatcaccaa cgaccaggac gtgctgagct tcaaggccct gtggaagaac ttcgacaacg
     3661 acctggaaag aaagagccgg ctgcggagcc tgatcctgaa gcacttcagc tttctggaag
     3721 gcgccgctta cggcaagaag ctgttcgagt ctaagagcag cggcaacaag agcagcaaga
     3781 acaaagagct gaccaagaaa gagaaagagg aactgcaggc caacgctctg agcctggaca
     3841 acctgaagtc tatcctgttc gacttcctgc agaagctgaa ggacttccgg aactactaca
     3901 gcgcctaccg gcacagcgga agcagcgagc tgcctctgtt cgacggcaac atgctgcagc
     3961 ggctgtacaa cgtgttcgac gtgtccgtgc agcgcgtgaa gatcgaccac gagcacaacg
     4021 atgaggtgga ccctcactac cacttcaacc acctcgtgcg gaagggcaag aaggacagat
     4081 acggccacaa cgacaacccc agcttcaagc accacttcgt ggacggcgaa ggcatggtta
     4141 cagaagccgg cctgctgttc ttcgtgtccc tgttcctgga aaagcgggac gccatctgga
     4201 tgcagaagaa gatccggggc ttcaaaggcg gcaccgagac ataccagcag atgaccaacg
     4261 aggtgttctg ccggtccaga atcagcctgc ctaagctgaa gctggaaagc ctgcggatgg
     4321 acgactggat gctgctggac atgctgaacg aactcgtgcg gtgccccaag cctctgtacg
     4381 acagactgag agaggacgac cgggcctgct ttagagtgcc cgtggatatc ctgcctgacg
     4441 aggacgatac agatggcggc ggagaggacc ccttcaagaa taccctcgtc cggcaccagg
     4501 acagattccc ttacttcgcc ctgcggtact tcgacctgaa gaaggtgttc accagcctgc
     4561 ggttccacat cgatctgggc acctaccact ttgccatcta caagaagatg atcggcgagc
     4621 agcccgagga cagacacctg accagaaacc tgtacggctt cggccggatc caggacttcg
     4681 ccgaagaaca cagacccgag gaatggaagc ggctcgtcag agatctggac tacttcgaga
     4741 caggcgacaa gccctacatc agccagacaa gcccacacta ccacatcgag aagggaaaga
     4801 tcggcctgag attcatgccc gagggccagc atctttggcc ctctccagaa gtgggcacca
     4861 ccagaaccgg cagatctaag tacgcccagg acaagagact gaccgccgag gcctttctgt
     4921 ctgtgcacga gctgatgcct atgatgttct actacttcct gctgcgcgag aagtacagcg
     4981 aggaagtgtc tgccgagaga gtgcagggca gaatcaagcg cgtgatcgag gatgtgtacg
     5041 ccgtgtacga tgcctttgcc agggacgaga tcaacacccg ggatgagctg gatgcctgcc
     5101 tggccgataa gggcatcaga agaggccatc tgcctcgcca gatgatcgcc atcctgagcc
     5161 aagagcacaa ggacatggaa gagaagattc ggaagaaact gcaagagatg atggccgaca
     5221 ccgaccaccg gctggatatg ctggatagac agaccgaccg gaagatcaga atcggccgga
     5281 agaatgccgg actgcctaag agcggagtga tcgccgattg gctcgtgcgg gacatgatga
     5341 gatttcagcc cgtggccaag gacgccagcg gaaagcctct gaacaactcc aaggccaaca
     5401 gcaccgagta ccggatgctg cagagagccc tggctctgtt tggaggcgag aaagagaggc
     5461 tgaccccata cttccggcag atgaatctga ccggcggaaa caaccctcat ccttttctgc
     5521 acgaaactcg ctgggagagc cacaccaaca tcctgtcctt ttaccggtcc tacctgagag
     5581 cccgcaaggc cttcctcgaa aggatcggca gatccgacag agtggaaaac cggccatttc
     5641 tgctgctgaa agagcccaag accgacagac agacactggt ggctggatgg aagggcgagt
     5701 tccatctgcc aagaggcatc ttcacagagg ccgtgcggga ttgcctgatc gagatgggac
     5761 acgatgaagt ggccagctac aaagaagtgg gattcatggc caaggccgtg ccactgtact
     5821 ttgagagagc ctgcgaggac agggtgcagc ccttttacga cagccccttc aacgtgggca
     5881 acagcctgaa gcctaagaag ggcagattcc tgagcaaaga ggaacgggcc gaagagtggg
     5941 agcggggcaa agagagattc cgggatctcg aagcctggtc ctatagcgcc gccagaagaa
     6001 tcgaggacgc ctttgccggc atcgagtatg ctagccccgg aaacaagaag aaaatcgagc
     6061 agctcctgcg ggacctgagc ctgtgggaag cctttgagag caagctgaaa gtgcgggccg
     6121 accggatcaa tctggccaag ctgaagaaag aaatcctgga agcccaagaa cacccctacc
     6181 acgacttcaa gagctggcag aagttcgagc gcgagctgcg gctggtcaag aaccaggata
     6241 tcatcacctg gatgatgtgc cgcgacctga tggaagaaaa caaggtggaa ggcctggaca
     6301 ccggcacact gtacctgaag gacatcagac ccaacgtgca agagcagggc agcctgaacg
     6361 tgctgaacag agtgaagccc atgagactgc ccgtggtggt gtacagagcc gatagcagag
     6421 gccacgtgca caaagaagaa gcccctctgg ccaccgtgta catcgaggaa agagacacca
     6481 agctgctgaa gcagggcaac ttcaagtcct tcgtgaagga caggcggctg aacggcctgt
     6541 tctccttcgt tgatactggc ggactggcca tggaacagta ccccatctcc aagctgagag
     6601 tggaatacga gctggccaag taccagaccg ccagagtgtg tgtgttcgag ctgaccctga
     6661 gactggaaga gtccctgctg acccggtatc ctcatctgcc cgacgagagc ttcagagaga
     6721 tgctggaatc ttggagcgac cctctgctgg ccaaatggcc tgaactgcac ggaaaagtgc
     6781 ggctgctgat cgccgtgcgg aatgccttta gcgccaatca gtaccctatg tacgacgagg
     6841 ccgtgttcag ctccatccgg aagtacgatc ctagcagccc cgacgccatc gaagagagaa
     6901 tgggcctgaa tatcgcccac cgcctgtctg aggaagtgaa gcaggccaaa gaaaccgtgg
     6961 aacggatcat tcaggccagt ctagaggcgc taccggtcgc caccatggtg tctaagggcg
     7021 aagagctgat caaggaaaat atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc
     7081 accaattcaa atgcacaggt gaaggagaag gcagaccgta cgagggagtg caaaccatga
     7141 ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt
     7201 tcatgtatgg cagccgtacc tttatcaagt acccggccga catccctgat ttctttaaac
     7261 agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg
     7321 tcaccgtcac gcaggacacc agccttgagg atggcgagct cgtctacaac gtcaaggtca
     7381 gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc
     7441 ctaatacaga gatgatgtat ccagcagatg gtggtctgag aggatacact gacatcgcac
     7501 tgaaagttga tggtggtggc catctgcact gcaacttcgt gacaacttac aggtcaaaaa
     7561 agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgatcaccgc ctggaaagga
     7621 tcgaggagag tgacaatgaa acctacgtag tgcaaagaga agtggcagtt gccaaataca
     7681 gcaaccttgg tggtggcatg gacgagctgt acaagtccgg actcagatct gtgagcaagg
     7741 gcgaggagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
     7801 gccaccaatt caaatgcaca ggtgaaggag aaggcagacc gtacgaggga gtgcaaacca
     7861 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
     7921 cgttcatgta tggcagccgt acctttatca agtacccggc cgacatccct gatttcttta
     7981 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
     8041 tcgtcaccgt cacgcaggac accagccttg aggatggcga gctcgtctac aacgtcaagg
     8101 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
     8161 agcctaatac agagatgatg tatccagcag atggtggtct gagaggatac actgacatcg
     8221 cactgaaagt tgatggtggt ggccatctgc actgcaactt cgtgacaact tacaggtcaa
     8281 aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc cgttgatcac cgcctggaaa
     8341 ggatcgagga gagtgacaat gaaacctacg tagtgcaaag agaagtggca gttgccaaat
     8401 acagcaacct tggtggtggc atggacgagc tgtacaagaa gcttgtgagc aagggcgagg
     8461 agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc
     8521 aattcaaatg cacaggtgaa ggagaaggca gaccgtacga gggagtgcaa accatgagga
     8581 tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca
     8641 tgtatggcag ccgtaccttt atcaagtacc cggccgacat ccctgatttc tttaaacagt
     8701 cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca
     8761 ccgtcacgca ggacaccagc cttgaggatg gcgagctcgt ctacaacgtc aaggtcagag
     8821 gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta
     8881 atacagagat gatgtatcca gcagatggtg gtctgagagg atacactgac atcgcactga
     8941 aagttgatgg tggtggccat ctgcactgca acttcgtgac aacttacagg tcaaaaaaga
     9001 ccgtcgggaa catcaagatg cccggtgtcc atgccgttga tcaccgcctg gaaaggatcg
     9061 aggagagtga caatgaaacc tacgtagtgc aaagagaagt ggcagttgcc aaatacagca
     9121 accttggtgg tggcatggac gagctgtaca agtccggact cagatctcga cggtaccgcc
     9181 atatgaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaatagcgtc
     9241 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9301 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9361 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     9421 tggtgcatga cccgcaagcc cggtgcctga cgcccatcga tagatcctaa tcaacctctg
     9481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9541 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     9601 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     9661 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9721 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9781 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9841 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9901 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9961 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10021 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
    10081 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    10141 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    10201 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10261 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10321 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    10381 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
    10441 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
    10501 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    10561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    10921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    11041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    11101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    11161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    11641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    11701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    11761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    11821 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    11881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    12001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    12061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    12121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    12181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    12241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    12301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    12361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    12421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    12481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    12541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    12601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    12661 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    12721 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    12781 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    12841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    12901 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    12961 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    13021 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
