LOCUS       Exported               12649 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12649)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12649
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..6927
                     /codon_start=1
                     /label=dLwaCas13a
                     /translation="KVTKVDGISHKKYIEEGKLVKSTSEENRTSERLSELLSIRLDIYI
                     KNPDNASEEENRIRRENLKKFFSNKVLHLKDSVLYLKNRKEKNAVQDKNYSEEDISEYD
                     LKNKNSFSVLKKILLNEDVNSEELEIFRKDVEAKLNKINSLKYSFEENKANYQKINENN
                     VEKVGGKSKRNIIYDYYRESAKRNDYINNVQEAFDKLYKKEDIEKLFFLIENSKKHEKY
                     KIREYYHKIIGRKNDKENFAKIIYEEIQNVNNIKELIEKIPDMSELKKSQVFYKYYLDK
                     EELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLN
                     KLDTYVRNCGKYNYYLQVGEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETEN
                     ENDITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKQNEVKENLKMFYSYDFNMDNKNEI
                     EDFFANIDEAISSIAHGIVAFNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLKI
                     FKQLNSANVFNYYEKDVIIKYLKNTKFNFVNKNIPFVPSFTKLYNKIEDLRNTLKFFWS
                     VPKDKEEKDAQIYLLKNIYYGEFLNKFVKNSKVFFKITNEVIKINKQRNQKTGHYKYQK
                     FENIEKTVPVEYLAIIQSREMINNQDKEEKNTYIDFIQQIFLKGFIDYLNKNNLKYIES
                     NNNNDNNDIFSKIKIKKDNKEKYDKILKNYEKHNRNKEIPHEINEFVREIKLGKILKYT
                     ENLNMFYLILKLLNHKELTNLKGSLEKYQSANKEETFSDELELINLLNLDNNRVTEDFE
                     LEANEIGKFLDFNENKIKDRKELKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLLEKI
                     ADKAKYKISLKELKEYSNKKNEIEKNYTMQQNLHRKYARPKKDEKFNDEDYKEYEKAIG
                     NIQKYTHLKNKVEFNELNLLQGLLLKILHRLVGYTSIWERDLRFRLKGEFPENHYIEEI
                     FNFDNSKNVKYKSGQIVEKYINFYKELYKDNVEKRSIYSDKKVKKLKQEKKDLYIANYI
                     AAFNYIPHAEISLLEVLENLRKLLSYDRKLKNAIMKSIVDILKEYGFVATFKIGADKKI
                     EIQTLESEKIVHLKNLKKKKLMTDRNSEELCELVKVMFEYKALEG"
     CDS             6952..7668
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7696..7743
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7762..7827
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7834..8401
                     /label=IRES
     CDS             8421..9020
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9039..9627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9510..9521)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9702..9935
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10140..10244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10245..11105
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11276..11864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12275..12296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12311..12341
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12349..12365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12373..12389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag caaagtgacc
     3481 aaggtcgacg gcatcagcca caagaagtac atcgaagagg gcaagctcgt gaagtccacc
     3541 agcgaggaaa accggaccag cgagagactg agcgagctgc tgagcatccg gctggacatc
     3601 tacatcaaga accccgacaa cgcctccgag gaagagaacc ggatcagaag agagaacctg
     3661 aagaagttct ttagcaacaa ggtgctgcac ctgaaggaca gcgtgctgta tctgaagaac
     3721 cggaaagaaa agaacgccgt gcaggacaag aactatagcg aagaggacat cagcgagtac
     3781 gacctgaaaa acaagaacag cttctccgtg ctgaagaaga tcctgctgaa cgaggacgtg
     3841 aactctgagg aactggaaat ctttcggaag gacgtggaag ccaagctgaa caagatcaac
     3901 agcctgaagt acagcttcga agagaacaag gccaactacc agaagatcaa cgagaacaac
     3961 gtggaaaaag tgggcggcaa gagcaagcgg aacatcatct acgactacta cagagagagc
     4021 gccaagcgca acgactacat caacaacgtg caggaagcct tcgacaagct gtataagaaa
     4081 gaggatatcg agaaactgtt tttcctgatc gagaacagca agaagcacga gaagtacaag
     4141 atccgcgagt actatcacaa gatcatcggc cggaagaacg acaaagagaa cttcgccaag
     4201 attatctacg aagagatcca gaacgtgaac aacatcaaag agctgattga gaagatcccc
     4261 gacatgtctg agctgaagaa aagccaggtg ttctacaagt actacctgga caaagaggaa
     4321 ctgaacgaca agaatattaa gtacgccttc tgccacttcg tggaaatcga gatgtcccag
     4381 ctgctgaaaa actacgtgta caagcggctg agcaacatca gcaacgataa gatcaagcgg
     4441 atcttcgagt accagaatct gaaaaagctg atcgaaaaca aactgctgaa caagctggac
     4501 acctacgtgc ggaactgcgg caagtacaac tactatctgc aagtgggcga gatcgccacc
     4561 tccgacttta tcgcccggaa ccggcagaac gaggccttcc tgagaaacat catcggcgtg
     4621 tccagcgtgg cctacttcag cctgaggaac atcctggaaa ccgagaacga gaacgatatc
     4681 accggccgga tgcggggcaa gaccgtgaag aacaacaagg gcgaagagaa atacgtgtcc
     4741 ggcgaggtgg acaagatcta caatgagaac aagcagaacg aagtgaaaga aaatctgaag
     4801 atgttctaca gctacgactt caacatggac aacaagaacg agatcgagga cttcttcgcc
     4861 aacatcgacg aggccatcag cagcatcgcc cacggcatcg tggccttcaa cctggaactg
     4921 gaaggcaagg acatcttcgc cttcaagaat atcgccccca gcgagatctc caagaagatg
     4981 tttcagaacg aaatcaacga aaagaagctg aagctgaaaa tcttcaagca gctgaacagc
     5041 gccaacgtgt tcaactacta cgagaaggat gtgatcatca agtacctgaa gaataccaag
     5101 ttcaacttcg tgaacaaaaa catccccttc gtgcccagct tcaccaagct gtacaacaag
     5161 attgaggacc tgcggaatac cctgaagttt ttttggagcg tgcccaagga caaagaagag
     5221 aaggacgccc agatctacct gctgaagaat atctactacg gcgagttcct gaacaagttc
     5281 gtgaaaaact ccaaggtgtt ctttaagatc accaatgaag tgatcaagat taacaagcag
     5341 cggaaccaga aaaccggcca ctacaagtat cagaagttcg agaacatcga gaaaaccgtg
     5401 cccgtggaat acctggccat catccagagc agagagatga tcaacaacca ggacaaagag
     5461 gaaaagaata cctacatcga ctttattcag cagattttcc tgaagggctt catcgactac
     5521 ctgaacaaga acaatctgaa gtatatcgag agcaacaaca acaatgacaa caacgacatc
     5581 ttctccaaga tcaagatcaa aaaggataac aaagagaagt acgacaagat cctgaagaac
     5641 tatgagaagc acaatcggaa caaagaaatc cctcacgaga tcaatgagtt cgtgcgcgag
     5701 atcaagctgg ggaagattct gaagtacacc gagaatctga acatgtttta cctgatcctg
     5761 aagctgctga accacaaaga gctgaccaac ctgaagggca gcctggaaaa gtaccagtcc
     5821 gccaacaaag aagaaacctt cagcgacgag ctggaactga tcaacctgct gaacctggac
     5881 aacaacagag tgaccgagga cttcgagctg gaagccaacg agatcggcaa gttcctggac
     5941 ttcaacgaaa acaaaatcaa ggaccggaaa gagctgaaaa agttcgacac caacaagatc
     6001 tatttcgacg gcgagaacat catcaagcac cgggccttct acaatatcaa gaaatacggc
     6061 atgctgaatc tgctggaaaa gatcgccgat aaggccaagt ataagatcag cctgaaagaa
     6121 ctgaaagagt acagcaacaa gaagaatgag attgaaaaga actacaccat gcagcagaac
     6181 ctgcaccgga agtacgccag acccaagaag gacgaaaagt tcaacgacga ggactacaaa
     6241 gagtatgaga aggccatcgg caacatccag aagtacaccc acctgaagaa caaggtggaa
     6301 ttcaatgagc tgaacctgct gcagggcctg ctgctgaaga tcctgcaccg gctcgtgggc
     6361 tacaccagca tctgggagcg ggacctgaga ttccggctga agggcgagtt tcccgagaac
     6421 cactacatcg aggaaatttt caatttcgac aactccaaga atgtgaagta caaaagcggc
     6481 cagatcgtgg aaaagtatat caacttctac aaagaactgt acaaggacaa tgtggaaaag
     6541 cggagcatct actccgacaa gaaagtgaag aaactgaagc aggaaaaaaa ggacctgtac
     6601 atcgccaact acattgccgc cttcaactac atcccccacg ccgagattag cctgctggaa
     6661 gtgctggaaa acctgcggaa gctgctgtcc tacgaccgga agctgaagaa cgccatcatg
     6721 aagtccatcg tggacattct gaaagaatac ggcttcgtgg ccaccttcaa gatcggcgct
     6781 gacaagaaga tcgaaatcca gaccctggaa tcagagaaga tcgtgcacct gaagaatctg
     6841 aagaaaaaga aactgatgac cgaccggaac agcgaggaac tgtgcgaact cgtgaaagtc
     6901 atgttcgagt acaaggccct ggaaggtcta gaggcgctac cggtcgccac catggtgagc
     6961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     7021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     7081 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     7141 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     7201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     7261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     7321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     7381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     7441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     7501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     7561 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     7621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc gaattctaag
     7681 ccaggattcc atatgaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag
     7741 aaacaacgcg tgcagctaag cgactacaaa gaccatgacg gtgattataa agatcatgac
     7801 atcgattaca aggatgacga tgacaagtaa ggatccctcc ccccccccta acgttactgg
     7861 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7921 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7981 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8041 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8101 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8161 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8221 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8281 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8341 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8401 aggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     8461 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     8521 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     8581 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     8641 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8701 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8761 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8821 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8881 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8941 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9001 cccgcaagcc cggtgcctga cgcccatcga tagatcctaa tcaacctctg gattacaaaa
     9061 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9121 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9181 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9241 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9301 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9361 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9421 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9481 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9541 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9601 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     9661 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9721 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
    10021 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
    10081 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10141 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10201 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10261 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10321 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10381 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10441 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    10501 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10561 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10621 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10681 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10741 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10801 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10861 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10921 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10981 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11041 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11101 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11941 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12001 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12061 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    12121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12181 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    12241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12361 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    12421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12481 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12541 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12601 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
