LOCUS       Exported               13507 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7782
                     /codon_start=1
                     /label=dLbaCas13a
                     /translation="MKISKVREENRGAKLTVNAKTAVVSENRSQEGILYNDPSRYGKSR
                     KNDEDRDRYIESRLKSSGKLYRIFNEDKNKRETDELQWFLSEIVKKINRRNGLVLSDML
                     SVDDRAFEKAFEKYAELSYTNRRNKVSGSPAFETCGVDAATAERLKGIISETNFINRIK
                     NNIDNKVSEDIIDRIIAKYLKKSLCRERVKRGLKKLLMNAFDLPYSDPDIDVQRDFIDY
                     VLEDFYHVRAKSQVSRSIKNMNMPVQPEGDGKFAITVSKGGTESGNKRSAEKEAFKKFL
                     SDYASLDERVRDDMLRRMRRLVVLYFYGSDDSKLSDVNEKFDVWEDHAARRVDNREFIK
                     LPLENKLANGKTDKDAERIRKNTVKELYRNQNIGCYRQAVKAVEEDNNGRYFDDKMLNM
                     FFIHRIEYGVEKIYANLKQVTEFKARTGYLSEKIWKDLINYISIKYIAMGKAVYNYAMD
                     ELNASDKKEIELGKISEEYLSGISSFDYELIKAEEMLQRETAVYVAFAARHLSSQTVEL
                     DSENSDFLLLKPKGTMDKNDKNKLASNNILNFLKDKETLRDTILQYFGGHSLWTDFPFD
                     KYLAGGKDDVDFLTDLKDVIYSMANDSFAYATENHNNGKWNKELISAMFEHETERMTVV
                     MKDKFYSNNLPMFYKNDDLKKLLIDLYKDNVERASQVPSFNKVFVRKNFPALVRDKDNL
                     GIELDLKADADKGENELKFYNALYYMFKEIYYNAFLNDKNVRERFITKATKVADNYDRN
                     KERNLKDRIKSAGSDEKKKLREQLQNYIAENDFGQRIKNIVQVNPDYTLAQICQLIMTE
                     YNQQNNGCMQKKSAARKDINKDSYQHYKMLLLVNLRKAFLEFIKENYAFVLKPYKHDLC
                     DKADFVPDFAKYVKPYAGLISRVAGSSELQKWYIVSRFLSPAQANHMLGFLHSYKQYVW
                     DIYRRASETGTEINHSIAEDKIAGVDITDVDAVIDLSVKLCGTISSEISDYFKDDEVYA
                     EYISSYLDFEYDGGNYKDSLNRFCNSDAVNDQKVALYYDGEHPKLNRNIILSKLYGERR
                     FLEKITDRVSRSDIVEYYKLKKETSQYQTKGIFDSEDEQKNIKKFQEMKNIVEFRDLMD
                     YSEIADELQGQLINWIYLRERDLMNFQLGYHYACLNNDSNKQATYVTLDYQGKKNRKIN
                     GAILYQICAMYINGLPLYYVDKDSSEWTVSDGKESTGAKIGEFYRYAKSFENTSDCYAS
                     GLEIFENISEHDNITELANYIEAFRYYSSFDRSFLGIYSEVFDRFFTYDLKYRKNVPTI
                     LYNILLQHFVNVRFEFVSGKKMIGIDKKDRKIAKEKECARITIREKNGVYSEQFTYKLK
                     NGTVYVDARDKRYLQSIIRLLFYPEKVNMDEMIEVKEKKKPSDNNTGKGYSKRDRQQDR
                     KEYDKYKEKKKKEGNFLSGMGGNINWDEINAQLKN"
     CDS             5271..5286
                     /label=HEPN1
     misc_feature    7201..7215
                     /label=HEPN2
     CDS             7810..8526
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8554..8601
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8620..8685
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8692..9259
                     /label=IRES
     CDS             9279..9878
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9897..10485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10368..10379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10560..10793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        10998..11102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11103..11963
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12134..12722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    13133..13154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13169..13199
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13207..13223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13231..13247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgaagatc
     3481 agcaaggtgc gggaggagaa cagaggcgcc aagctgaccg tgaatgccaa gacagccgtg
     3541 gtgtctgaga accggagcca ggagggcatc ctgtataatg acccaagcag gtacggcaag
     3601 tcccgcaaga acgacgagga tagggaccgc tatatcgaga gcagactgaa gagctccggc
     3661 aagctgtacc ggatctttaa cgaggataag aataagcgcg agacagacga gctgcagtgg
     3721 ttcctgtccg agatcgtgaa gaagatcaac cggagaaatg gcctggtgct gtccgacatg
     3781 ctgtctgtgg acgatagagc cttcgagaag gcctttgaga agtacgccga gctgtcttat
     3841 accaacaggc gcaataaggt gtccggctct cccgccttcg agacatgcgg agtggatgca
     3901 gcaacagcag agcggctgaa gggaatcatc tccgagacaa acttcatcaa cagaatcaag
     3961 aacaacatcg acaacaaggt gtctgaggac atcatcgatc ggatcatcgc caagtacctg
     4021 aagaagagcc tgtgccggga gagagtgaag agaggcctga agaagctgct gatgaatgcc
     4081 tttgatctgc catactctga tcccgacatc gatgtgcaga gggacttcat cgattatgtg
     4141 ctggaggact tttaccacgt gcgggccaag agccaggtga gcagatccat caagaacatg
     4201 aatatgcccg tgcagcctga gggcgacggc aagttcgcca tcaccgtgag caagggcggc
     4261 acagagtctg gcaacaagcg cagcgccgag aaggaggcct tcaagaagtt tctgagcgat
     4321 tacgcctccc tggacgagag ggtgcgcgac gatatgctgc ggagaatgag gcgcctggtg
     4381 gtgctgtact tttatggctc cgacgattct aagctgagcg atgtgaacga gaagttcgac
     4441 gtgtgggagg accacgcagc ccggagagtg gacaataggg agttcatcaa gctgccactg
     4501 gagaacaagc tggccaatgg caagaccgac aaggatgccg agcggatcag aaagaacaca
     4561 gtgaaggagc tgtatagaaa ccagaatatc ggctgctaca ggcaggccgt gaaggccgtg
     4621 gaggaggaca acaatggccg gtactttgac gataagatgc tgaacatgtt ctttatccac
     4681 agaatcgagt atggcgtgga gaagatctac gccaatctga agcaggtgac cgagttcaag
     4741 gcccgcacag gctacctgag cgagaagatc tggaaggatc tgatcaacta catctctatc
     4801 aagtatatcg ccatgggcaa ggccgtgtat aactatgcca tggatgagct gaatgcctcc
     4861 gacaagaagg agatcgagct gggcaagatc tccgaggagt atctgtctgg catctctagc
     4921 ttcgactacg agctgatcaa ggccgaggag atgctgcaga gggagacagc cgtgtacgtg
     4981 gcctttgccg caaggcacct gtcctctcag acagtggagc tggattccga gaactctgac
     5041 ttcctgctgc tgaagcctaa gggcaccatg gacaagaacg ataagaataa gctggcctcc
     5101 aacaatatcc tgaattttct gaaggataag gagacactgc gggacacaat cctgcagtat
     5161 ttcggcggcc actctctgtg gacagatttc ccatttgaca agtacctggc cggcggcaag
     5221 gacgatgtgg attttctgac cgacctgaag gatgtgatct atagcatggc gaacgactcc
     5281 ttcgcctacg ccacagagaa ccacaacaat ggcaagtgga ataaggagct gatctccgcc
     5341 atgtttgagc acgagacaga gagaatgaca gtggtcatga aggacaagtt ctattctaac
     5401 aatctgccca tgttttacaa gaacgacgat ctgaagaagc tgctgatcga cctgtataag
     5461 gacaatgtgg agagagcctc tcaggtgccc agcttcaaca aggtgtttgt gcgcaagaat
     5521 ttccctgccc tggtgcggga caaggataac ctgggcatcg agctggatct gaaggccgac
     5581 gccgataagg gcgagaatga gctgaagttc tacaacgccc tgtactacat gttcaaggag
     5641 atctactaca acgccttcct gaacgacaag aatgtgaggg agcggttcat caccaaggcc
     5701 acaaaggtgg ccgacaacta tgataggaat aaggagcgca acctgaagga taggatcaag
     5761 agcgccggct ccgacgagaa gaagaagctg cgcgagcagc tgcagaatta catcgccgag
     5821 aacgatttcg gccagaggat caagaatatc gtgcaggtga accctgacta taccctggcc
     5881 cagatctgcc agctgatcat gacagagtac aaccagcaga acaatggctg tatgcagaag
     5941 aagagcgccg cccggaagga tatcaataag gactcctacc agcactataa gatgctgctg
     6001 ctggtgaacc tgagaaaggc cttcctggag tttatcaagg agaattatgc ctttgtgctg
     6061 aagccctaca agcacgacct gtgcgataag gccgacttcg tgcctgattt tgccaagtac
     6121 gtgaagccct acgccggcct gatcagcagg gtggcaggca gctccgagct gcagaagtgg
     6181 tatatcgtgt cccgctttct gtctcctgcc caggccaacc acatgctggg cttcctgcac
     6241 tcctacaagc agtacgtgtg ggacatctac aggcgcgcct ctgagacagg caccgagatc
     6301 aatcacagca tcgccgagga taagatcgcc ggcgtggaca tcaccgacgt ggatgccgtg
     6361 atcgatctga gcgtgaagct gtgcggcaca atctctagcg agatctccga ctacttcaag
     6421 gacgatgagg tgtacgccga gtatatctcc tcttacctgg attttgagta tgacggcggc
     6481 aactacaagg atagcctgaa taggttctgt aactccgatg ccgtgaatga ccagaaggtg
     6541 gccctgtact atgacggcga gcaccctaag ctgaaccgca atatcatcct gagcaagctg
     6601 tacggcgagc ggagatttct ggagaagatc accgatcggg tgagccggag cgacatcgtg
     6661 gagtactata agctgaagaa ggagacaagc cagtaccaga caaagggcat cttcgatagc
     6721 gaggacgagc agaagaacat caagaagttt caggagatga agaatatcgt ggagttcaga
     6781 gatctgatgg actatagcga gatcgccgat gagctgcagg gccagctgat caactggatc
     6841 tacctgcggg agcgggacct gatgaatttc cagctgggct accactatgc ctgcctgaac
     6901 aatgattcca acaagcaggc cacctatgtg acactggact accagggcaa gaagaatcgg
     6961 aagatcaacg gcgccatcct gtatcagatc tgtgccatgt atatcaatgg cctgcctctg
     7021 tactatgtgg acaaggatag ctccgagtgg accgtgtctg acggcaagga gagcacaggc
     7081 gccaagatcg gcgagttcta cagatatgcc aagtcctttg agaacacctc tgattgctac
     7141 gccagcggcc tggagatctt tgagaatatc agcgagcacg acaacatcac agagctggcc
     7201 aattatatcg aggcctttcg ctactacagc agcttcgacc ggagcttcct gggcatctac
     7261 tctgaggtgt tcgatcggtt ctttacctac gacctgaagt atagaaagaa cgtgccaaca
     7321 atcctgtata acatcctgct gcagcacttc gtgaacgtga ggttcgagtt cgtgagcggc
     7381 aagaagatga tcggcatcga taagaaggac cgcaagatcg ccaaggagaa ggagtgtgcc
     7441 cggatcacca tcagagagaa gaacggcgtg tatagcgagc agtttaccta caagctgaag
     7501 aatggcacag tgtatgtgga tgccagggac aagagatacc tgcagtccat catcaggctg
     7561 ctgttctatc cagagaaggt gaacatggat gagatgatcg aggtgaagga gaagaagaag
     7621 ccctccgaca acaataccgg caagggctac tctaagaggg atcgccagca ggaccgcaag
     7681 gagtacgata agtataagga gaagaagaag aaggagggca atttcctgag cggcatgggc
     7741 ggcaacatca attgggacga gatcaatgcc cagctgaaga acagtctaga ggcgctaccg
     7801 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     7861 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     7921 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     7981 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     8041 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     8101 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     8161 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     8221 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     8281 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     8341 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     8401 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     8461 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8521 tacaagtcga attctaagcc aggattccat atgaagcgac ctgccgccac aaagaaggct
     8581 ggacaggcta agaagaagaa acaacgcgtg cagctaagcg actacaaaga ccatgacggt
     8641 gattataaag atcatgacat cgattacaag gatgacgatg acaagtaagg atccctcccc
     8701 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     8761 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     8821 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     8881 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     8941 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     9001 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     9061 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     9121 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     9181 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     9241 ttgaaaaaca cgatgataag gatccaccgg agcttaccat gaccgagtac aagcccacgg
     9301 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     9361 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     9421 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     9481 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     9541 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     9601 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     9661 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     9721 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     9781 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     9841 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccatcgata gatcctaatc
     9901 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9961 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    10021 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    10081 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    10141 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    10201 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    10261 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    10321 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    10381 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    10441 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
    10501 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    10561 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
    10621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    10801 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    10861 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
    10921 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
    10981 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    11041 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    11101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    11161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    11221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11341 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11821 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    12001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    12061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    12301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12901 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
    12961 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    13021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    13081 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    13141 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    13201 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
    13261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    13321 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    13381 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    13441 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    13501 tagtagt
//
