LOCUS       Exported               12468 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    3432..3437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3441..3461
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     misc_feature    3460..3461
                     /label=dpspcas13b
     CDS             3462..6743
                     /codon_start=1
                     /label=dRanCas13b
                     /translation="EKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPS
                     NDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQK
                     EQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSAYKHKKPDAEKDIFKHLY
                     KAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLL
                     FFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQML
                     LDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFA
                     LRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRP
                     QEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIA
                     KYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIE
                     DLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSS
                     PKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALY
                     GGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQP
                     WEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIK
                     KHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPT
                     ESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYH
                     QLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHT
                     KALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETL
                     SLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCANQYPF
                     YKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI"
     primer_bind     4213..4232
                     /label=Ran-seq-F1
     primer_bind     complement(5988..6006)
                     /label=Ran-seq-R1
     primer_bind     complement(6723..6768)
                     /label=Ran-EGFP-XbaI-R
     CDS             6771..7487
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7515..7562
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7581..7646
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7653..8220
                     /label=IRES
     CDS             8240..8839
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8858..9446
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9329..9340)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9521..9754
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9959..10063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10064..10924
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11095..11683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12094..12115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12130..12160
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12168..12184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12192..12208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag cgccaccatg cccaaaaaga agaggaaagt ggagaagccc ctgctgccta
     3481 acgtgtatac cctgaagcac aagttctttt ggggcgcctt cctgaatatc gcccggcaca
     3541 acgcctttat caccatctgc cacatcaatg agcagctggg cctgaaaacc cccagcaacg
     3601 acgataagat cgtggacgtg gtgtgcgaga catggaacaa tatcctgaac aatgaccacg
     3661 atctgctgaa gaagtcccag ctgaccgagc tgatcctgaa gcacttccct tttctgacag
     3721 ccatgtgcta ccacccccct aagaaggagg gcaagaagaa gggccaccag aaggagcagc
     3781 agaaggagaa ggagagcgag gcacagtccc aggcagaggc cctgaaccca tccaagctga
     3841 tcgaggccct ggagatcctg gtgaatcagc tgcactctct gcggaactac tatagcgcct
     3901 ataagcacaa gaagcccgac gccgagaagg atatcttcaa gcacctgtat aaggccttcg
     3961 acgcctctct gagaatggtg aaggaggatt ataaggccca cttcaccgtg aatctgacaa
     4021 gggactttgc ccacctgaac cgcaagggca agaacaagca ggacaatccc gacttcaaca
     4081 gatacagatt cgagaaggat ggcttcttta ccgagtctgg cctgctgttc tttacaaatc
     4141 tgtttctgga caagagggat gcctattgga tgctgaagaa ggtgtccggc ttcaaggcct
     4201 ctcacaagca gcgcgagaag atgaccacag aggtgttttg caggagccgc atcctgctgc
     4261 ccaagctgag gctggagtcc cgctacgacc acaaccagat gctgctggat atgctgtctg
     4321 agctgagcag atgtcctaag ctgctgtatg agaagctgag cgaggagaat aagaagcact
     4381 tccaggtgga ggccgacggc tttctggatg agatcgagga ggagcagaac ccattcaagg
     4441 acaccctgat ccggcaccag gatagattcc cctactttgc cctgaggtat ctggacctga
     4501 atgagtcctt caagtctatc cgctttcagg tggatctggg cacataccac tattgtatct
     4561 acgacaagaa gatcggcgat gagcaggaga agaggcacct gacccgcaca ctgctgtcct
     4621 tcggccggct gcaggacttt accgagatca acagacccca ggagtggaag gccctgacca
     4681 aggacctgga ttacaaggag acatccaatc agcctttcat ctctaagacc acaccacact
     4741 atcacatcac cgacaacaag atcggctttc ggctgggcac aagcaaggag ctgtacccct
     4801 ccctggagat caaggatggc gccaatagaa tcgccaagta cccttataac tccggcttcg
     4861 tggcccacgc ctttatctct gtgcacgagc tgctgcctct gatgttctac cagcacctga
     4921 ccggcaagag cgaggacctg ctgaaggaga cagtgcggca catccagaga atctataagg
     4981 acttcgagga ggagcggatc aataccatcg aggatctgga gaaggcaaac cagggcagac
     5041 tgccactggg agcctttccc aagcagatgc tgggcctgct gcagaataag cagcctgatc
     5101 tgtccgagaa ggccaagatc aagatcgaga agctgatcgc cgagacaaag ctgctgtctc
     5161 acaggctgaa cacaaagctg aagagctccc caaagctggg caagcggaga gagaagctga
     5221 tcaagacagg cgtgctggcc gactggctgg tgaaggattt catgcgcttt cagcccgtgg
     5281 cctacgacgc ccagaatcag cctatcaagt ctagcaaggc caactccacc gagttctggt
     5341 ttatcaggcg cgccctggcc ctgtacggag gagagaagaa taggctggag ggctatttca
     5401 agcagaccaa cctgatcggc aacacaaatc cacacccctt cctgaacaag tttaattgga
     5461 aggcctgcag gaatctggtg gatttctacc agcagtatct ggagcagcgc gagaagtttc
     5521 tggaggccat caagaaccag ccatgggagc cctaccagta ttgcctgctg ctgaagatcc
     5581 ctaaggagaa caggaagaat ctggtgaagg gatgggagca gggaggaatc tctctgccac
     5641 ggggcctgtt taccgaggcc atcagagaga cactgtctga ggacctgatg ctgagcaagc
     5701 caatccgcaa ggagatcaag aagcacggcc gggtgggctt catcagcaga gccatcaccc
     5761 tgtactttaa ggagaagtat caggataagc accagagctt ctacaatctg tcctataagc
     5821 tggaggcaaa ggcaccactg ctgaagcggg aggagcacta cgagtattgg cagcagaaca
     5881 agcctcagtc tccaaccgag agccagaggc tggagctgca cacaagcgac cgctggaagg
     5941 attacctgct gtataagaga tggcagcacc tggagaagaa gctgcggctg taccggaatc
     6001 aggacgtgat gctgtggctg atgaccctgg agctgacaaa gaaccacttc aaggagctga
     6061 acctgaatta tcaccagctg aagctggaga atctggccgt gaacgtgcag gaggccgatg
     6121 ccaagctgaa ccctctgaat cagaccctgc ccatggtgct gcctgtgaag gtgtaccctg
     6181 ccacagcctt tggcgaggtg cagtaccaca agaccccaat caggacagtg tatatccgcg
     6241 aggagcacac caaggccctg aagatgggca acttcaaggc cctggtgaag gaccggagac
     6301 tgaatggcct gttctctttt atcaaggagg agaacgatac acagaagcac ccaatcagcc
     6361 agctgcggct gaggcgcgag ctggagatct accagtctct gagagtggac gccttcaagg
     6421 agacactgag cctggaggag aagctgctga ataagcacac atccctgtcc tctctggaga
     6481 acgagtttag ggccctgctg gaggagtgga agaaggagta tgccgccagc tccatggtga
     6541 cagacgagca catcgccttc atcgccagcg tgcgcaatgc cttctgcgcc aaccagtacc
     6601 ccttctacaa ggaggccctg cacgccccta tcccactgtt caccgtggcc cagcccacca
     6661 cagaggagaa ggatggcctg ggaatcgcag aggccctgct gaaggtgctg agggagtact
     6721 gtgagatcgt gaagtcccag atcggtctag aggcgctacc ggtcgccacc atggtgagca
     6781 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6841 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6901 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6961 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7021 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7081 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7141 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7201 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7261 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7321 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7381 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7441 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcg aattctaagc
     7501 caggattcca tatgaagcga cctgccgcca caaagaaggc tggacaggct aagaagaaga
     7561 aacaacgcgt gcagctaagc gactacaaag accatgacgg tgattataaa gatcatgaca
     7621 tcgattacaa ggatgacgat gacaagtaag gatccctccc ccccccctaa cgttactggc
     7681 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     7741 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     7801 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     7861 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     7921 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     7981 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     8041 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     8101 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     8161 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     8221 ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8281 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8341 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8401 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     8461 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8521 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8581 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8641 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8701 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8761 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8821 ccgcaagccc ggtgcctgac gcccatcgat agatcctaat caacctctgg attacaaaat
     8881 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8941 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     9001 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     9061 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     9121 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     9181 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     9241 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     9301 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     9361 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     9421 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     9481 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9541 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9721 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9781 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     9841 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     9901 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
    10321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    11101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    11161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    11221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    11281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    11341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    11521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    11881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt
    11941 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
    12001 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc
    12061 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    12121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    12181 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt
    12241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    12301 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    12361 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    12421 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
