LOCUS       Exported               12058 bp ds-DNA     circular SYN 09-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 9, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12058
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..6333
                     /codon_start=1
                     /label=ESCasR
                     /translation="MGKKIHARDLREQRKTDRTEKFADQNKKREAERAVPKKDAAVSVK
                     SVSSVSSKKDNVTKSMAKAAGVKSVFAVGNTVYMTSFGRGNDAVLEQKIVDTSHEPLNI
                     DDPAYQLNVVTMNGYSVTGHRGETVSAVTDNPLRRFNGRKKDEPEQSVPTDMLCLKPTL
                     EKKFFGKEFDDNIHIQLIYNILDIEKILAVYSTNAIYALNNMSADENIENSDFFMKRTT
                     DETFDDFEKKKESTNSREKADFDAFEKFIGNYRLAYFADAFYVNKKNPKGKAKNVLRED
                     KELYSVLTLIGKLAHWCVASEEGRAEFWLYKLDELKDDFKNVLDVVYNRPVEEINNRFI
                     ENNKVNIQILGSVYKNTDIAELVRSYYEFLITKKYKNMGFSIKKLRESMLEGKGYADKE
                     YDSVRNKLYQMTDFILYTGYINEDSDRADDLVNTLRSSLKEDDKTTVYCKEADYLWKKY
                     RESIREVADALDGDNIKKLSKSNIEIQEDKLRKCFISYADSVSEFTKLIYLLTRFLSGK
                     EINDLVTTLINKFDNIRSFLEIMDELGLDRTFTAEYSFFEGSTKYLAELVELNSFVKSC
                     SFDINAKRTMYRDALDILGIESDKTEEDIEKMIDNILQIDANGDKKLKKNNGLRNFIAS
                     NVIDSNRFKYLVRYGNPKKIRETAKCKPAVRFVLNEIPDAQIERYYEACCPKNTALCSA
                     NKRREKLADMIAEIKFENFSDAGNYQKANVTSRTSEAEIKRKNQAIIRLYLTVMYIMLK
                     NLVNVNARYVIAFHCVERDTKLYAESGLEVGNIEKNKTNLTMAVMGVKLENGIIKTEFD
                     KSFAENAANRYLRNARWYKLILDNLKKSERAVVNEFANTVCALNAIRNININIKEIKEV
                     ENYFALYHYLIQKHLENRFADKKVERDTGDFISKLEEHKTYCKDFVKAYCTPFGYNLVR
                     YKNLTIDGLFDKNYPGKDDSDEQK"
     misc_feature    4357..4371
                     /label=HEPN
     misc_feature    6019..6033
                     /label=HEPN
     CDS             6361..7077
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7105..7152
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7171..7236
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7243..7810
                     /label=IRES
     CDS             7830..8429
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8448..9036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8919..8930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9111..9344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9549..9653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9654..10514
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10685..11273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11684..11705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11720..11750
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11758..11774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11782..11798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgggcaag
     3481 aagatccacg cccgggacct gagagagcag aggaagaccg accggacaga gaagttcgcc
     3541 gatcagaaca agaagaggga ggcagagagg gcagtgccca agaaggacgc cgccgtgagc
     3601 gtgaagtccg tgagctccgt gtctagcaag aaggataatg tgaccaagtc tatggccaag
     3661 gcagcaggag tgaagagcgt gttcgcagtg ggcaacaccg tgtacatgac atcctttggc
     3721 agaggcaatg acgccgtgct ggagcagaag atcgtggata catctcacga gccactgaac
     3781 atcgacgatc ccgcctacca gctgaatgtg gtgaccatga acggctattc cgtgacagga
     3841 cacaggggag agaccgtgtc tgccgtgaca gacaacccac tgcggagatt caatggccgc
     3901 aagaaggacg agcccgagca gtctgtgcct accgatatgc tgtgcctgaa gcccacactg
     3961 gagaagaagt tctttggcaa ggagtttgac gataacatcc acatccagct gatctacaat
     4021 atcctggaca tcgagaagat cctggccgtg tacagcacca acgccatcta tgccctgaac
     4081 aatatgagcg ccgacgagaa tatcgagaac tccgatttct ttatgaagag gaccacagat
     4141 gagaccttcg acgattttga gaagaagaag gagtctacaa acagccggga gaaggccgac
     4201 ttcgatgcct ttgagaagtt catcggcaat tacagactgg cctattttgc cgacgccttc
     4261 tacgtgaata agaagaaccc caagggcaag gccaagaatg tgctgcgcga ggataaggag
     4321 ctgtattctg tgctgaccct gatcggcaag ctggcccact ggtgcgtggc cagcgaggag
     4381 ggaagagcag agttctggct gtacaagctg gacgagctga aggacgactt caagaacgtg
     4441 ctggatgtgg tgtataatag gcctgtggag gagatcaaca atcgcttcat cgagaacaat
     4501 aaggtgaaca tccagatcct gggcagcgtg tacaagaata ccgacatcgc cgagctggtg
     4561 agatcctact atgagttcct gatcacaaag aagtataaga acatgggctt ttccatcaag
     4621 aagctgcggg agtctatgct ggagggcaag ggctacgccg acaaggagta tgattctgtg
     4681 agaaataagc tgtaccagat gaccgacttc atcctgtaca caggctatat caacgaggac
     4741 agcgataggg ccgacgatct ggtgaatacc ctgcgctcct ctctgaagga ggacgataag
     4801 accacagtgt attgcaagga ggccgactac ctgtggaaga agtatcggga gtccatcaga
     4861 gaggtggccg atgccctgga cggcgataac atcaagaagc tgagcaagtc caatatcgag
     4921 atccaggagg acaagctgag gaagtgtttt atcagctacg ccgattctgt gagcgagttc
     4981 accaagctga tctatctgct gacacgcttt ctgtccggca aggagatcaa cgacctggtg
     5041 accacactga tcaataagtt tgataacatc aggtctttcc tggagatcat ggacgagctg
     5101 ggcctggatc gcaccttcac agccgagtac agcttctttg agggctccac caagtatctg
     5161 gccgagctgg tggagctgaa cagctttgtg aagtcctgct ctttcgacat caatgccaag
     5221 aggaccatgt accgcgacgc cctggatatc ctgggcatcg agtccgacaa gacagaggag
     5281 gatatcgaga agatgatcga caacatcctg cagatcgacg ccaatggcga taagaagctg
     5341 aagaagaaca atggcctgcg gaatttcatc gcctctaacg tgatcgatag caatcggttt
     5401 aagtacctgg tgagatatgg caaccctaag aagatcaggg agaccgccaa gtgcaagcct
     5461 gccgtgcgct tcgtgctgaa tgagatccca gatgcccaga tcgagcggta ctatgaggcc
     5521 tgctgtccaa agaacaccgc cctgtgcagc gccaataaga ggcgcgagaa gctggccgac
     5581 atgatcgccg agatcaagtt tgagaacttc tccgatgccg gcaactacca gaaggccaat
     5641 gtgacctcca gaacatctga ggccgagatc aagaggaaga accaggccat catccgcctg
     5701 tacctgaccg tgatgtatat catgctgaag aatctggtga acgtgaatgc cagatacgtg
     5761 atcgcctttc actgcgtgga gagagacaca aagctgtatg ccgagagcgg cctggaggtc
     5821 ggcaacatcg agaagaataa gaccaacctg acaatggccg tgatgggcgt gaagctggag
     5881 aatggcatca tcaagaccga gtttgacaag tccttcgccg agaatgccgc caaccgctac
     5941 ctgcggaacg ccagatggta taagctgatc ctggataatc tgaagaagtc tgagcgggcc
     6001 gtggtgaatg agttcgccaa caccgtgtgc gccctgaacg ccatcaggaa catcaatatc
     6061 aacatcaagg agatcaagga ggtggagaac tactttgccc tgtaccacta tctgatccag
     6121 aagcacctgg agaatcggtt cgccgacaag aaggtggaga gagacaccgg cgacttcatc
     6181 tccaagctgg aggagcacaa gacctactgc aaggactttg tgaaggccta ttgtacaccc
     6241 ttcggctaca acctggtgcg ctataagaat ctgacaatcg acggcctgtt cgataagaac
     6301 taccctggca aggacgatag cgacgagcag aagggtctag aggcgctacc ggtcgccacc
     6361 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6541 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6661 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6721 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6781 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6841 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6901 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6961 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7021 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcg
     7081 aattctaagc caggattcca tatgaagcga cctgccgcca caaagaaggc tggacaggct
     7141 aagaagaaga aacaacgcgt gcagctaagc gactacaaag accatgacgg tgattataaa
     7201 gatcatgaca tcgattacaa ggatgacgat gacaagtaag gatccctccc ccccccctaa
     7261 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     7321 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     7381 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     7441 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     7501 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     7561 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     7621 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     7681 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     7741 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     7801 acgatgataa ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     7861 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7921 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7981 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     8041 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     8101 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     8161 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     8221 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     8281 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8341 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8401 ggtgcatgac ccgcaagccc ggtgcctgac gcccatcgat agatcctaat caacctctgg
     8461 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8521 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8581 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8641 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8701 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8761 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8821 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8881 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8941 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9001 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     9061 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9121 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     9181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     9361 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     9421 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     9481 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     9541 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9601 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9661 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9721 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9781 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9841 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     9901 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9961 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10021 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10081 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10141 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10201 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10261 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10321 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10381 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10441 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10501 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10561 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10621 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10681 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10741 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10801 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10861 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10921 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10981 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11041 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11101 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11161 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11221 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11281 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11341 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11401 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11461 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
    11521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    11581 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
    11641 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11701 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11761 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
    11821 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11881 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    11941 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    12001 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
