LOCUS       pSNAPf-H3_zeo.xdna    5453 bp      DNA     circular   8/16/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(3292..3312)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(3680..4308)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(3318..3340)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(4460..5119)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5359..5387)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(3345..3374)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2577..2945
                     /label=Zeocin
                     /SerialCloner_Color=&hAC6EC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1971..2553
                     /label=EMCV IRES
                     /SerialCloner_Color=&h221AC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1545..1949
                     /label=H3
                     /SerialCloner_Color=&hC0941A
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    251..817
                     /label=CMV
                     /SerialCloner_Color=&h2C34C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    969..1514
                     /label=SNAP
                     /SerialCloner_Color=&hC06248
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat cgtcgctagc gatatcggcg cgccagcatt taaatctgta cagaccggtg 
      961 aattcaccat ggacaaagac tgcgaaatga agcgcaccac cctggatagc cctctgggca 
     1021 agctggaact gtctgggtgc gaacagggcc tgcaccgtat catcttcctg ggcaaaggaa 
     1081 catctgccgc cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc ggaccagagc 
     1141 cactgatgca ggccaccgcc tggctcaacg cctactttca ccagcctgag gccatcgagg 
     1201 agttccctgt gccagccctg caccacccag tgttccagca ggagagcttt acccgccagg 
     1261 tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt catcagctac agccacctgg 
     1321 ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa aaccgccctg agcggaaatc 
     1381 ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg cgacctggac gtggggggct 
     1441 acgagggcgg gctcgccgtg aaagagtggc tgctggccca cgagggccac agactgggca 
     1501 agcctgggct gggtcctgca ggcggatccg cgtttaaact cgaggctcgc actaagcaaa 
     1561 ctgctcggaa gtctactggt ggcaaggcgc cacgcaaaca gttggccact aaggcagccc 
     1621 gcaaaagcgc tccggccacc ggcggcgtga aaaagcccca ccgctaccgg ccgggcaccg 
     1681 tggctctgcg cgagatccgc cgttatcaga agtccactga actgcttatt cgtaaactac 
     1741 ctttccagcg cctggtgcgc gagattgcgc aggactttaa aacagacctg cgtttccaga 
     1801 gctccgctgt gatggctctg caggaggcgt gcgaggccta cttggtaggg ctatttgagg 
     1861 acactaacct gtgcgccatc cacgccaagc gcgtcactat catgcccaag gacatccagc 
     1921 tcgcccgccg catccgcgga gagagggcgt gactctcccc cccccccttt tccctccccc 
     1981 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg 
     2041 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc 
     2101 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg 
     2161 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg 
     2221 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 
     2281 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 
     2341 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 
     2401 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 
     2461 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt 
     2521 tgaaaaacac gatgataagc ttgccacaac ccggtataat tcctgcagcc aatATGGCCA 
     2581 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT 
     2641 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC 
     2701 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GATGGTGCCG GACAACACCC 
     2761 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT 
     2821 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG 
     2881 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC 
     2941 AGGACTGACA CGTGCtctag ataactgatc ataatcagcc ataccacatt tgtagaggtt 
     3001 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 
     3061 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     3121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     3181 atcaatgtat cttaacgcgt cgagtgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     3241 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
     3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 
     3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
     5461 
     5521 
//