LOCUS       pSNAPf-macroH2A_zeo2.xdna    6501 bp      DNA     circular   8/16/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    924..2039
                     /label=macroH2A
                     /SerialCloner_Color=&hC01B6E
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4340..4360)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4728..5356)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4366..4388)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5508..6167)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6407..6435)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4393..4422)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     intron          2675..2970
                     /label=synth int
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3625..3993
                     /label=Zeocin
                     /SerialCloner_Color=&hAC6EC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3019..3601
                     /label=EMCV IRES
                     /SerialCloner_Color=&h221AC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    251..817
                     /label=CMV
                     /SerialCloner_Color=&h2C34C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2055..2600
                     /label=SNAP
                     /SerialCloner_Color=&hC06248
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat cgatatcgaa ttgATGTCGA GCCGCGGCGG GAAGAAGAAA TCCACCAAGA 
      961 CCTCCCGGTC AGCCAAGGCC GGAGTCATCT TCCCTGTGGG ACGCATGCTT CGGTACATCA 
     1021 AGAAAGGCCA CCCTAAGTAT AGGATCGGAG TGGGGGCACC TGTGTACATG GCTGCTGTCC 
     1081 TGGAGTACCT GACTGCTGAG ATCCTGGAGC TGGCTGGCAA TGCAGCAAGA GACAACAAGA 
     1141 AGGGACGGGT CACACCCCGG CACATCCTGT TAGCTGTGGC CAATGATGAA GAGCTAAACC 
     1201 AGCTGCTAAA GGGTGTCACC ATAGCCAGCG GGGGCGTGTT GCCGAATATC CATCCTGAGT 
     1261 TGCTAGCGAA GAAGCGAGGA TCCAAGGGAA AATTGGAAGC CATCATCACG CCTCCGCCGG 
     1321 CCAAAAAGGC CAAGTCTCCA TCCCAGAAGA AGCCAGTGGC TAAGAAGACA GGAGGCAAGA 
     1381 AAGGGGCCCG GAAGTCTAAG AAGAAGCAGG GAGAAGTGAG CAAGGCGGCC AGCGCAGACA 
     1441 GTACGACGGA GGGCACGCCT ACAGACGGCT TCACTGTCCT CTCCACCAAG AGCCTCTTCC 
     1501 TCGGCCAGAA GCTGAACCTT ATTCACAGTG AAATCAGTAA TTTAGCCGGC TTTGAGGTGG 
     1561 AGGCCATAAT CAATCCTACC AATGCTGACA TTGACCTTAA AGATGACCTA GGAAACACAC 
     1621 TGGAGAAGAA GGGCGGCAAG GAGTTTGTAG AAGCTGTTCT GGAACTCCGG AAAAAGAACG 
     1681 GGCCCTTGGA GGTAGCTGGA GCTGCTATTA GTGCAGGCCA TGGCCTGCCT GCCAAGTTTG 
     1741 TGATCCACTG TAATAGTCCT GTCTGGGGTG CAGACAAATG TGAAGAACTT CTAGAAAAGA 
     1801 CGGTGAAAAA CTGCTTGGCT CTAGCTGATG ACAGAAAGCT GAAATCCATC GCCTTCCCAT 
     1861 CCATTGGCAG CGGCAGGAAC GGGTTCCCGA AGCAGACAGC GGCCCAGCTC ATTCTGAAGG 
     1921 CCATCTCCAG CTACTTTGTC TCCACGATGT CCTCCTCCAT CAAAACTGTG TACTTCATGC 
     1981 TTTTTGACAG TGAGAGCATA GGTATCTATG TGCAGGAAAT GGCCAAGCTG GACGCCAACa 
     2041 ccggtgaatt caccatggac aaagactgcg aaatgaagcg caccaccctg gatagccctc 
     2101 tgggcaagct ggaactgtct gggtgcgaac agggcctgca ccgtatcatc ttcctgggca 
     2161 aaggaacatc tgccgccgac gccgtggaag tgcctgcccc agccgccgtg ctgggcggac 
     2221 cagagccact gatgcaggcc accgcctggc tcaacgccta ctttcaccag cctgaggcca 
     2281 tcgaggagtt ccctgtgcca gccctgcacc acccagtgtt ccagcaggag agctttaccc 
     2341 gccaggtgct gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc 
     2401 acctggccgc cctggccggc aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg 
     2461 gaaatcccgt gcccattctg atcccctgcc accgggtggt gcagggcgac ctggacgtgg 
     2521 ggggctacga gggcgggctc gccgtgaaag agtggctgct ggcccacgag ggccacagac 
     2581 tgggcaagcc tgggctgggt cctgcaggcg gatccgcgtt taaactcgag gttaattaat 
     2641 gagcggccgc atagataact gatccagtgt gctggaatta attcgctgtc tgcgagggcc 
     2701 agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca 
     2761 gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg 
     2821 gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg 
     2881 cttgagatct ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag 
     2941 gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc 
     3001 ctctcccccc cccccttttc cctccccccc ccctaacgtt actggccgaa gccgcttgga 
     3061 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 
     3121 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 
     3181 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 
     3241 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 
     3301 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 
     3361 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 
     3421 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 
     3481 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 
     3541 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc 
     3601 ggtataattc ctgcagccaa tATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG 
     3661 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC 
     3721 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC 
     3781 CAGGACCAGA TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG 
     3841 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC 
     3901 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC 
     3961 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCtctagat aactgatcat 
     4021 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 
     4081 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 
     4141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     4201 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtcg agtgcattct 
     4261 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     4321 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     4381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     4441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     4501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     4561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     4621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     4681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     4741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     4801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     4861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     4921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     4981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     5041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     5101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     5161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     5221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     5281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     5341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     5401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     5461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     5521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     5581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     5641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     5701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     5761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     5821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     5881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     5941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     6001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     6061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     6121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     6181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     6241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     6301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     6361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     6421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     6481 cgaaaagtgc cacctgacgt c
     6541 
//