LOCUS       Exported                4527 bp ds-DNA     circular SYN 20-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2019 from SnapGene Viewer 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      72..754
                     /label=ColE1 origin
     misc_recomb     1982..2015
                     /label=LoxP
     misc_feature    2046..2053
                     /label=PmeI
     misc_feature    2096..2101
                     /label=SphI
     misc_feature    2132..2221
                     /label=polh
     misc_feature    2271..3875
                     /label=WDR34
     polyA_signal    complement(3967..4158)
                     /label=SV40 late polyA
     misc_feature    4171..4178
                     /label=SwaI
     misc_feature    4187..4194
                     /label=PmeI
                     /label=PmeI(1)
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttgttgta cctatgggca tggcacaatt ctagcgcatg
     2101 ccatcctggg tactgttcat atcgtgcaag aatggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg gcaacaaggg
     2281 ctcaacctgg tcctctctct caagctggat ctgctggcgt ggctgctctg gctaccgtcg
     2341 gagtggcttc tggacccggt cctggcagac ccggaccact gcaagacgaa accctcggcg
     2401 tggcttccgt gccttctcag tggagagccg tccagggaat tagaggcgaa actaagtcat
     2461 gccagactgc atcaatcgct accgcctctg cctccgctca agccagaaat catgtggatg
     2521 ctcaggtgca gaccgaggca cctgtccctg tgtcagtgca gcctccatct cagtatgaca
     2581 tccccaggct cgcagccttc ctgcgcagag tcgaggctat ggtgatcagg gagctgaaca
     2641 agaactggca atcccacgct ttcgatggat tcgaggtcaa ttggactgaa cagcagcaga
     2701 tggttagctg cctgtataca ctgggttatc ctcctgctca ggctcagggc ctgcacgtca
     2761 cttctatctc atggaactca accggctcag tcgtcgcctg tgcttacggt aggctggacc
     2821 acggtgactg gtccacactc aagtcatttg tctgtgcttg gaacctcgac cgcagggacc
     2881 tgaggccaca acagccaagc gctgtcgtgg aggtccctag cgctgtcctg tgcctggcct
     2941 tccatcccac acagccaagc catgttgctg gtggactgta ctctggtgag gtcctggtgt
     3001 gggacctctc acgcctcgaa gatcccctcc tctggagaac tggcctgact gatgacaccc
     3061 acaccgaccc agtgagccag gtcgtctggc tcccagaacc cggtcactcc cacaggtttc
     3121 aagtcctcag cgtggccaca gacggcaagg tcttgttgtg gcagggcatc ggcgtcggcc
     3181 agttgcagct gaccgaaggc ttcgccctgg tgatgcaaca gctgcctagg tctactaaac
     3241 tcaagaaaca cccaagaggc gaaaccgagg ttggtgctac cgcagttgcc ttttcctctt
     3301 tcgatccaag actcttcatt ctgggtactg agggaggctt ccctctgaag tgttccctcg
     3361 ctgccggtga agctgctctg acaaggatgc ctagctccgt gcccctgagg gctcctgccc
     3421 agtttacctt cagcccacat ggtggaccca tctacagcgt gagctgctct ccattccacc
     3481 gcaacctgtt tctgagcgca ggaaccgacg gccacgttca cttgtactct atgctccaag
     3541 cacctcccct gacctcattg caactgtctc tgaagtactt gttcgccgtt aggtggtccc
     3601 ctgttcgccc actggtgttt gccgcagctt ccggaaaggg cgatgtccag ctgttcgatt
     3661 tgcagaaatc ttctcaaaag cccacagttt tgatcaagca gactcaagac gagagcccag
     3721 tgtattgttt ggagtttaac tcccagcaga cccagctgtt ggctgctggt gatgctcagg
     3781 gtactgttaa agtttggcag ttgagcacag agtttacaga acagggtcct cgcgaagccg
     3841 aggatttgga ttgcctggca gctgaggtgg ctgcatgact agagcctgca gtctcgacaa
     3901 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     3961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4141 tgtatcttat catgtctgga aacatccacc atttaaatgg agttaggttt aaacggatct
     4201 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
     4261 gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca tctaattgga
     4321 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     4381 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     4441 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg ttgacttggg
     4501 tcaactgtca gaccaagttt actcata
//
