LOCUS       Exported                3972 bp ds-DNA     circular SYN 20-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2019 from SnapGene Viewer 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      72..754
                     /label=ColE1 origin
     misc_recomb     1982..2015
                     /label=LoxP
     misc_feature    2046..2053
                     /label=PmeI
     misc_feature    2096..2101
                     /label=KpnI
     misc_feature    2132..2221
                     /label=polh
     misc_feature    2271..3320
                     /label=hs_LIC3
     polyA_signal    complement(3412..3603)
                     /label=SV40 late polyA
     misc_feature    3616..3623
                     /label=SwaI
     misc_feature    3632..3639
                     /label=PmeI
                     /label=PmeI(1)
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttgatcac tactttgctt aggcgagaga gtctcggtac
     2101 ctaaccgagt gagcgtatta ctcctagcgt tatggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg ccttcagaaa
     2281 cattgtggga aatcgctaaa gcagaggtgg aaaagagggg aatcaatgga agcgagggag
     2341 acggtgccga gatcgccgaa aagttcgtgt tctttatcgg ctcaaagaac ggtggaaaga
     2401 ctacaatcat cctcaggtgt ctggaccgcg atgagcctcc caaaccaacc ctcgctctcg
     2461 aatacactta cggaagaaga gccaagggtc acaacactcc caaagatatt gcccactttt
     2521 gggagttggg aggtggcacc tcactcctcg acctgatctc cattcccatt actggcgata
     2581 ccctgcgcac cttctccttg gtgttggtgc tggatctctc aaaaccaaat gatctgtggc
     2641 ctacaatgga gaacctgctg caagctacta agtcccacgt ggataaggtg attatgaagc
     2701 tgggtaaaac aaacgcaaag gcagtttctg agatgaggca aaagatttgg aacaacatgc
     2761 ctaaggacca ccccgatcac gaactgatcg accccttccc cgtgccactg gtgatcatcg
     2821 gaagcaagta cgacgtgttt caagatttcg agtcagaaaa gcgcaaagtg atctgcaaaa
     2881 cattgagatt cgtggcccat tactatggtg catcattgat gtttacatcc aagtcagaag
     2941 ccctcttgtt gaagatcaga ggtgtgatta accagttggc cttcggtatt gacaagtcta
     3001 agtccatttg cgttgatcag aataagcccc tctttatcac tgccggactg gattcttttg
     3061 gacaaattgg ctctccaccc gtgcctgaga atgacatcgg aaagctgcac gcacattctc
     3121 caatggaatt gtggaagaag gtctatgaga agctcttccc tcctaagagc attaacaccc
     3181 tgaaagacat taaggaccca gctcgcgacc ctcagtatgc cgagaacgag gtggacgaga
     3241 tgcgcatcca aaaggacctg gagctggaac agtacaagcg ctcatcctcc aaatcctgga
     3301 agcagatcga actggactct tgactagagc ctgcagtctc gacaagcttg tcgagaagta
     3361 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3421 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3601 ctggaaacat ccaccattta aatggagtta ggtttaaacg gatctgatca ctgcttgagc
     3661 ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     3721 gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa
     3781 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     3841 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     3901 cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac tgtcagacca
     3961 agtttactca ta
//
