LOCUS       Exported                3345 bp ds-DNA     circular SYN 20-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 14, 2019 from SnapGene Viewer 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      72..754
                     /label=ColE1 origin
     misc_recomb     1982..2015
                     /label=LoxP
     misc_feature    2046..2053
                     /label=PmeI
     misc_feature    2096..2101
                     /label=XbaI
     misc_feature    2132..2221
                     /label=polh
     misc_feature    2271..2693
                     /label=hsTCTEX-2
     polyA_signal    complement(2785..2976)
                     /label=SV40 late polyA
     misc_feature    2989..2996
                     /label=SwaI
     misc_feature    3005..3012
                     /label=PmeI
                     /label=PmeI(1)
ORIGIN
        1 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
       61 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
      841 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1081 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1141 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1201 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1261 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1321 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1381 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1441 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1501 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1561 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1621 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1681 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1741 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1801 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1861 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1921 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     1981 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
     2041 gcgacgttta aacatccacc atttgtgctg ataatggatt ttcaatcgac gcgcctctag
     2101 aagcgcacca cactttgagt tgggtctaat tatggagata attaaaatga taaccatctc
     2161 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2221 attccggatt attcataccg tcccaccatc gggcgcggat cctcaacatg gctacctcca
     2281 tcggcgtgtc cttctccgtg ggcgacggtg tccctgaggc tgagaagaac gctggcgagc
     2341 ccgagaacac ctacatcctg cgtcccgtgt tccagcagcg tttccgtccc tccgtggtca
     2401 aggactgcat ccacgctgtg ctgaaggaag gactggctaa cgctgagtac tcccccgagg
     2461 aaatgcccca gctgaccaag cacctgtccg agaacatcaa ggacaagctc aaggaaatgg
     2521 gcttcgaccg ctacaagatg gtggtgcagg tcgtgatcgg cgagcagcgt ggcgaaggcg
     2581 tgttcatggc ttcccgttgt ttctgggacg ctgacaccga caactacacc cacgacgttt
     2641 tcatgaacga ctccctgttc tgcgtggtgg ctgctttcgg ttgcttctac tactaactag
     2701 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     2761 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2821 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2881 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2941 tttgtccaaa ctcatcaatg tatcttatca tgtctggaaa catccaccat ttaaatggag
     3001 ttaggtttaa acggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc
     3061 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggg
     3121 tatacccatc taattggaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     3181 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     3241 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     3301 acaaccggtt gacttgggtc aactgtcaga ccaagtttac tcata
//
