LOCUS       GS2.1_EC               16355 bp ds-DNA     circular SYN 21-AUG-2019
DEFINITION  pds3 CRISPR-Cas9 with egg cell promoter.
ACCESSION
VERSION     .
KEYWORDS    pN_35S/mCitrine.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Pribil and Mathias
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid
            protein CURT1A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..13
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     14..33
                     /standard_name="pUC119 gRNA-cas9 extraction (1 F) -
                     pH2GW7,0 extraction"
     promoter        76..379
                     /standard_name="At U6"
     misc_RNA        380..399
                     /standard_name="AtPDS3 gRNA"
     misc_RNA        400..476
                     /standard_name="gRNA template"
     terminator      477..482
                     /standard_name="gRNA terminator"
     misc_feature    625..654
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     terminator      complement(655..880)
                     /standard_name="35S terminator"
     misc_feature    complement(890..>916)
                     /note="Geneious type: attR2"
     CDS             complement(917..>1642)
                     /translation="MGGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSSMVSKGEEN
                     NMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDIL
                     SPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFI
                     YKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKSEIKKRLKLKDGGHYAAEV
                     KTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDELYK*"
                     /standard_name="mApple fusion"
     misc_feature    1628..1657
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     exon            complement(<1643..4354)
                     /standard_name="pcoCas9"
     intron          complement(4355..4543)
                     /standard_name="Potato IV2 intron"
     exon            complement(4544..6058)
                     /standard_name="pcoCas9"
     misc_feature    complement(6059..6606)
                     /note="Geneious type: promoter"
                     /standard_name="EC1.1p"
     primer_bind     6059..6082
                     /standard_name="EC1.1p (reversed) (1 F) - GS2 vector"
     misc_feature    complement(6611..7410)
                     /note="Geneious type: promoter"
                     /standard_name="EC1.2en"
     protein_bind    complement(7549..7573)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /standard_name="attB2"
     misc_feature    7893..7917
                     /note="right border repeat from nopaline C58 T-DNA"
                     /standard_name="RB T-DNA repeat"
     CDS             9248..9877
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
                     /standard_name="pVS1 StaA"
     CDS             10306..11379
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                      pVS1 (Heeb et al., 2000)"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
                     /standard_name="pVS1 RepA"
     rep_origin      11445..11639
     primer_bind     complement(11875..11897)
                     /note="pGEX vectors, reverse primer"
                     /standard_name="pGEX 3'"
     misc_feature    11983..12123
                     /note="basis of mobility region from pBR322"
                     /standard_name="bom"
     primer_bind     12038..12057
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /standard_name="pRS-marker"
     primer_bind     complement(12138..12155)
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     rep_origin      complement(12309..12897)
     primer_bind     complement(12389..12408)
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     CDS             complement(13143..13934)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     misc_feature    14455..14479
                     /note="left border repeat from nopaline C58 T-DNA"
                     /standard_name="LB T-DNA repeat"
     terminator      14572..14824
                     /note="nopaline synthase terminator and poly(A) signal"
                     /standard_name="NOS terminator"
     CDS             complement(15022..16044)
                     /standard_name="hpt"
     promoter        complement(16080..16259)
                     /note="nopaline synthase promoter"
                     /standard_name="NOS promoter"
     misc_feature    16355^16356
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CTCCCATATGGTCGACTAGAGCCAAGCTGATCTCCTTTGCCC
                     CGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGG
                     AGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTC
                     AGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGA
                     CGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGA
                     TGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTC
                     AAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGC
                     AAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGA
                     GTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTC
                     ATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGC
                     ACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGC
                     TAT"
ORIGIN      
        1 ctcccatatg gtcgccatcg ctaccttagg accgttatag ttaattaccc tgttatccct
       61 attaattaag agctcagaaa tctcaaaatt ccggcagaac aattttgaat ctcgatccgt
      121 agaaacgaga cggtcattgt tttagttcca ccacgattat atttgaaatt tacgtgagtg
      181 tgagtgagac ttgcataaga aaataaaatc tttagttggg aaaaaattca ataatataaa
      241 tgggcttgag aaggaagcga gggataggcc tttttctaaa ataggcccat ttaagctatt
      301 aacaatcttc aaaagtacca cagcgcttag gtaaagaaag cagctgagtt tatatatggt
      361 tagagacgaa gtagtgattg gacttttgcc agccatggtg ttttagagct agaaatagca
      421 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      481 ttctagaccc agctttcttg tacaaagttg gcattaccca tcggcgcgcc tctagaattt
      541 aaatggatcc tacgtactcg aggaattcac tggccgtcgt tttacaacgt cgtgactggg
      601 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccaaggtca
      661 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
      721 acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt
      781 cccttatctg ggaactactc acacattatt ctggagaaaa atagagagag atagatttgt
      841 agagagagac tggtgatttt tgcggactct agcatggccg cgggatatca ccactttgta
      901 caagaaagct gaacgattac ttgtacagct cgtccatgcc gccggtggag tggcggccct
      961 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggacacg atgtccaact
     1021 tgatgtcgac gatgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     1081 tgacctcggc ggcgtagtgg ccgccgtcct tcagcttcag cctcttcttg atctcgctct
     1141 tcagggcgcc gtcctcgggg tacatccgct cctcggaggc ctcccagccc atggtcttct
     1201 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     1261 acacgccgtc ctgcagggag gagtcctggt taacgtgaat aatgccgccg tcctcgaagt
     1321 tcatcacgcg ctcccacctg aagccctcgg ggaaggacag cttgaagtag tcggggatgt
     1381 cggctgggtg cttaatgtag accttggagc cgtacatgaa ctgaggggac aggatgtccc
     1441 aggcgaaggg cagggggcca cccttggtca ccttcagctt agcggtctga aaggcctcgt
     1501 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     1561 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttattctcc tcgcccttgc
     1621 tcaccataga acctccacct cccttcttct tcttagcctg tccagccttc ttggtagcag
     1681 ctggtctctt atctcctcca agctgagaaa gatcgattct ggtctcgtaa agtccggtga
     1741 tagactggtg gataagggta gcatcaagaa cctccttggt agaggtgtat ctttttctat
     1801 cgatggtggt atcgaagtac ttgaaagcag ctggagcacc aaggttggta agggtgaaaa
     1861 ggtggatgat gttctcagcc tgctctctga ttggcttatc tctgtgcttg ttgtaagcag
     1921 aaagaacctt atcaaggtta gcatcagcaa ggataactct cttagagaac tcagagattt
     1981 gctcgatgat ctcatcaagg tagtgcttgt gctgctcaac gaaaagctgc ttctgctcgt
     2041 tatcctctgg agatcccttc aacttctcgt agtgagaagc aaggtaaagg aagttaacgt
     2101 acttagatgg aagagcaagc tcgtttccct tctgaagctc tccagcagaa gcaagcattc
     2161 tctttcttcc gttctcaagc tcgaaaagag agtactttgg caacttgatg ataagatcct
     2221 tcttaacctc cttgtatccc ttagcctcaa ggaaatcgat tgggttcttc tcgaaagaag
     2281 aacgctccat gatggtgatt ccaagaagct ccttaacaga cttcaacttc ttagactttc
     2341 ccttctcaac cttagcaaca acaagaacag agtaagcaac ggttggagaa tcgaatcctc
     2401 cgtacttctt tgggtcccaa tccttctttc tagcgatcaa cttatcagag tttctctttg
     2461 gaaggataga ctccttagag aatcctccgg tctgaacctc ggttttctta acgatgttaa
     2521 cctgtggcat agaaagaacc tttctaacgg tagcgaaatc tcttccctta tcccaaacga
     2581 tctctccggt ctctccgttg gtctcgataa gtggtctctt tctgatctct ccgttagcaa
     2641 gggtgatctc ggtcttgaag aagttcatga tgttagagta gaagaagtac ttagcggtag
     2701 cctttccgat ctcctgctca gacttagcga tcatctttct aacatcgtaa accttgtaat
     2761 ctccgtaaac gaactcagac tccaactttg ggtacttctt gataagagcg gttccaacaa
     2821 cagcgttaag gtaagcatcg tgagcgtggt ggtagttgtt gatctctcta accttgtaga
     2881 actggaaatc ctttctgaaa tcagaaacca acttagactt caaggtgata accttaacct
     2941 ctctgatcaa cttatcgttc tcatcgtact tggtgttcat acgagaatca aggatctgag
     3001 caacgtgctt ggtgatctgt ctggtctcaa caagctgtct cttgatgaat ccagccttat
     3061 caagctcaga aagtcctcct ctctcagcct tggtaaggtt atcgaacttt ctctgggtga
     3121 tcaacttagc gttaagaagc tgtctccagt agttcttcat cttcttaaca acctcctcag
     3181 atggaacgtt atcagacttt cctctgttct tatcagaacg ggtaagaacc ttgttatcga
     3241 tagaatcatc cttcaagaaa gactgtggaa cgatgtgatc aacatcgtaa tcagaaagtc
     3301 tgttgatgtc aagctcctga tcaacgtaca tatctcttcc gttctgaagg tagtaaaggt
     3361 acaacttctc gttctgaagc tgggtgttct caactgggtg ctccttcaag atttgagatc
     3421 caagctcctt gattccctcc tcgattctct tcattctctc acgagagttc ttctgtccct
     3481 tctgggtggt ctggttctct ctagccatct cgataacgat gttctctggc ttgtgtctac
     3541 ccataacctt aacaagctca tcaacaacct taacggtctg aaggattccc ttcttgatag
     3601 ctggagatcc agcaaggtta gcgatgtgct cgtgaagaga atctccctgt ccagaaacct
     3661 gagccttctg gatgtcctcc ttgaaggtaa gagaatcatc gtggataagc tgcatgaagt
     3721 ttctgttagc gaatccatca gacttcaaga aatcaaggat ggtctttcca gactgcttat
     3781 ctctgattcc gttgatcaac ttacgagaaa gtctacccca tccggtgtat cttcttctct
     3841 tcaactgctt cataacctta tcatcgaaaa ggtgagcgta ggtcttgagt ctctcctcga
     3901 tcatctctct atcctcgaaa agggtaaggg taagaacgat gtcctcaagg atgtcctcgt
     3961 tctcctcgtt atcaaggaaa tccttatcct tgatgatctt caaaagatcg tggtaggttc
     4021 caagagaagc gttgaatcta tcctcaactc cagagatttc aacagaatcg aagcactcga
     4081 tcttcttgaa gtaatcctcc ttcaactgct taacggtaac ctttctgttg gtcttgaaaa
     4141 gaagatcaac gatagccttc ttctgctctc cagaaaggaa agctggcttt ctcattccct
     4201 cggtaacgta cttaaccttg gtaagctcgt tgtaaacggt gaagtactcg taaagaagag
     4261 agtgctttgg aagaaccttc tcgtttggaa ggttcttatc gaagttggtc attctctcga
     4321 tgaaagactg agcagaagct cccttatcaa caacctgcac atcaacaaat tttggtcata
     4381 tattagaaaa gttataaatt aaaatataca cacttataaa ctacagaaaa gcaattgcta
     4441 tatactacat tcttttattt tgaaaaaaat atttgaaata ttatattact actaattaat
     4501 gataattatt atatatatat caaaggtaga agcagaaact tacctcctcg aagttccaag
     4561 gggtgatggt ctcctcagac tttctggtca tccaagcgaa acgagagttt cctctagcaa
     4621 gtggtccaac gtagtatggg attctgaagg taaggatctt ctcgatcttc tctctgttat
     4681 ccttcaagaa tgggtagaaa tcctcctgtc tacgaaggat agcgtgaagc tctccaaggt
     4741 ggatctggtg tgggatagat ccgttatcga aggttctctg ctttctaaga agatcctctc
     4801 tgttcaactt aacaagaagc tcctcggttc catccatctt ctcaaggatt ggcttgatga
     4861 acttgtagaa ctcctcctga gaagctcctc catcgatgta tccagcgtat ccgttcttag
     4921 actgatcgaa gaagatttcc ttgtacttct ctggaagctg ctgtctaaca agagccttca
     4981 aaagggtaag atcctggtgg tgctcatcgt atctcttgat catagaagca gaaagtggag
     5041 ccttggtgat ctcggtgtta actctaagga tgtcagaaag aaggatagca tcagaaaggt
     5101 tcttagcagc aaggaaaaga tcagcgtact gatctccgat ctgagcaaga aggttatcaa
     5161 gatcatcatc gtaggtatcc ttagaaagct gcaacttagc atcctcagca agatcgaagt
     5221 tagacttgaa gtttggggta agtccaagag aaagagcgat aaggtttccg aaaagtccgt
     5281 tcttcttctc tcctggaagc tgagcgataa ggttctcaag tctacgagac ttagaaagtc
     5341 tagcagaaag gatagcctta gcatcaactc cagaagcgtt gattgggttc tcctcgaaaa
     5401 gctggttgta ggtctgaaca agctggatga acaacttatc aacatcagag ttatctgggt
     5461 taaggtctcc ctcgataagg aagtgtcctc tgaacttgat catgtgagca agagcaaggt
     5521 agataagtct aagatcagcc ttatcggtag aatcaaccaa cttctttcta aggtggtaga
     5581 tggttgggta cttctcgtgg taagcaacct catcaacgat gtttccgaag attgggtgtc
     5641 tctcgtgctt cttatcctcc tcaacaagga aagactcctc aagtctgtgg aagaaagaat
     5701 catcaacctt agccatctcg ttagagaaga tttcctgaag gtagcagatt ctgttctttc
     5761 ttctggtgta tcttcttcta gcggttctct tcaatctggt agcctcagcg gtctctccag
     5821 aatcgaaaag aagagcaccg ataaggttct tcttgataga gtgtctatcg gtgtttccaa
     5881 gaaccttgaa cttcttagat ggaaccttgt actcatcggt gataacagcc catccaacag
     5941 agttggttcc gatgtcaagt ccgatagagt acttcttatc agcagctgga actccgtgga
     6001 ttccaacctt tctcttcttc tttggagcca tcttatcatc atcatccttg taatccattt
     6061 ctcaacagat tgataaggtc gaaagaagaa agagtagaag ttgctttggt tgatttgttt
     6121 gtgatgagaa ttgtgtgtta actttagaga gtttatatag agacagtaga gacttgactg
     6181 ttacaaataa atggtagagt gaaagcccaa gtaattacga gggaagctca ttagattgtg
     6241 agataattag tgcttagtgg tttagtgggt aaactaggtg taattactct attaaaagct
     6301 gcgtaattag tgggtctgtt taggggctaa tgggccggat tagaatcact cagtctgaaa
     6361 ctcgtttgat ttagttcaat agaaccaaat cagctttacc atcaaaatct ttgcgagaaa
     6421 atagatttgg gtaagtaaag tatgtacaca tctaatgagc atgtgtgagc atgaaatcta
     6481 taactatttg atcatcttga aaccgaagaa atcataaggc aactatttta aagacacaga
     6541 gacacctgca atactgcaaa tttgaaaggt tgctagattt agtagagcta attcatgata
     6601 ggcgttacta gcttagtggt gatttaagag taattaaaaa gttggattag tgggaggaat
     6661 gggaaaggat tgacggtaaa tagtcactta atcatgaaac gttaaagcat ttgactgtag
     6721 aaacgtattt agtatatatt ggcatgttga atcagtagcg ctcctattgg agacgaaggt
     6781 ttaacgtcgt gttacagaaa gtgaaccaat gcttttgtaa taaactcatt gaactttatg
     6841 ctatatactg tatatggttt ttactactca ttgaacttta tgctatgttt tctttaaact
     6901 ccttgtataa atatatttct taggaattac ttttaaatta tgtacaaaga catgtattac
     6961 agaaaaattt catgttgtaa tctaaataag atgctatagt gttgtcgatg tgtcatgtgt
     7021 gtcactatcc atcattaaac atgtggtaaa attacagtct ataaccatca cctagatcag
     7081 atatatcttt ttaagaattt aaacttatgg tcaaattttc acaatctcga atacaaatat
     7141 acaagaatgt atattctcaa gtctcctagt ttctctgtta tttgcatctt ttgtgactta
     7201 caaactatga taacttttat aaaatagata taattagcta tcttcttgga caagttacct
     7261 ctcttaggtt gtttctgttg tttactatgc tctgattctt aacaaaaaat ggtttatttt
     7321 gttctgataa tggttttaag ctattccagc tcagtggatc tctgtccttg tataaacgca
     7381 atgataaacc aaacgcaaat gcttttattc tggcgtaata gcgaagaggc ccgcaccgat
     7441 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     7501 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaacctcgt tcagctttct
     7561 tgtacaaagt ggtgatatcc cgcggccatg ctagagtccg caaaaatcac cagtctctct
     7621 ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag
     7681 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     7741 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     7801 agtgacctgc aggcatgcga cgtcgggccc aagcttagct tgagcttgga tcagattgtc
     7861 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     7921 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     7981 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtacttta
     8041 aagtacttta aagtacttta aagtactttg atccaacccc tccgctgcta tagtgcagtc
     8101 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     8161 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     8221 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     8281 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     8341 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     8401 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     8461 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     8521 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     8581 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     8641 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     8701 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     8761 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     8821 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     8881 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     8941 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     9001 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     9061 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     9121 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     9181 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     9241 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     9301 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     9361 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     9421 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     9481 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     9541 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     9601 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     9661 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     9721 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     9781 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     9841 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     9901 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     9961 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
    10021 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
    10081 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
    10141 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
    10201 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
    10261 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
    10321 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
    10381 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
    10441 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
    10501 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
    10561 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
    10621 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
    10681 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
    10741 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
    10801 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
    10861 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
    10921 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
    10981 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
    11041 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
    11101 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
    11161 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
    11221 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    11281 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    11341 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    11401 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
    11461 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    11521 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    11581 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    11641 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    11701 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    11761 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    11821 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    11881 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    11941 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    12001 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    12061 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    12121 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    12181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    12241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    12301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    12421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12721 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    12781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12961 attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa
    13021 ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc
    13081 taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga
    13141 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
    13201 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
    13261 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
    13321 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
    13381 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
    13441 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
    13501 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
    13561 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
    13621 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
    13681 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
    13741 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
    13801 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
    13861 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
    13921 accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
    13981 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
    14041 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
    14101 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
    14161 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc ccgaattgat
    14221 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    14281 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    14341 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    14401 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    14461 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    14521 tttaatgtac tgaattaacg ccgaattgaa ttatcagctt gcatgccggt cgatctagta
    14581 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
    14641 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
    14701 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
    14761 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
    14821 gatctgcttg actctaggga attaattcct gaatcactgc gaccggccct cccgcgaccc
    14881 agccgagcga gcttagcgaa ctgtggacga gaactgtgcc accaagcgta aggccgttct
    14941 ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg aggcgttctc gaaatcagct
    15001 cttgttcggt cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt
    15061 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
    15121 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
    15181 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
    15241 aatgcggagc atatacgccc ggagccttgg cgatcctgca agctccggat gcctccgctc
    15301 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
    15361 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
    15421 gccattgtcc gtcaggacat tgttggagcc gaatccgcgt gcacgagtgc cggacttcgg
    15481 ggcagtcctc gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
    15541 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
    15601 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
    15661 gctaagatcg gccgcagcga tagcatccat agcctccgcg accggctgaa gaacagcggg
    15721 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
    15781 ggtcaggctc tcgctgaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
    15841 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
    15901 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
    15961 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
    16021 cgcggtgagt tcaggctttt tcataggggg gattcgagtt gagagtgaat atgagactct
    16081 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
    16141 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
    16201 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
    16261 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
    16321 ccttaattct ccgctcatga tcgataattc gagct
//
