LOCUS       pDNR-tomNeo  8140 bp    DNA   circular            
DEFINITION  Complementary copy of pDNR-Dual.
SOURCE      
  ORGANISM  
COMMENT     http://www.invitrogen.com/
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618527311|
COMMENT     VNTAUTHORNAME|Tel: (301) 216-0586|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(7006..8921)
                     /dnas_title="SacB gene"
                     /vntifkey="4"
                     /label=SacB gene
     misc_feature    complement(5394..5867)
                     /dnas_title="pUC Ori"
                     /vntifkey="21"
                     /label=pUC Ori
     CDS             complement(6018..6875)
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     CDS             8992..9651
                     /dnas_title="CmR"
                     /vntifkey="4"
                     /label=CmR
     CDS             1169..2596
                     /dnas_title="tdTomato"
                     /vntifkey="4"
                     /label=tdTomato
     promoter        2900..3409
                     /dnas_title="PGK"
                     /vntifkey="29"
                     /label=PGK
     promoter        3415..3488
                     /dnas_title="gb"
                     /vntifkey="30"
                     /label=gb
     CDS             3489..4292
                     /dnas_title="NeoR"
                     /vntifkey="4"
                     /label=NeoR
     misc_feature    2854..2888
                     /dnas_title="FRT"
                     /vntifkey="21"
                     /label=FRT
     misc_feature    4430..4464
                     /dnas_title="FRT"
                     /vntifkey="21"
                     /label=FRT
     polyA_signal    4338..4414
                     /dnas_title="pA"
                     /vntifkey="25"
                     /label=pA
     polyA_signal    2752..2802
                     /dnas_title="SV40 pA"
                     /vntifkey="25"
                     /label=SV40 pA
     source          1..9883
                     /dnas_title="pDNR-MYH11-NLuc-2A-tomNeo"
     misc_feature    14..559
                     /note="MYH11-5'Arm"
     misc_feature    4470..5140
                     /note="MYH11-3'Arm"
     misc_feature    566..1078
                     /note="NLuc"
     misc_feature    1079..1144
                     /note="2A"
ORIGIN      
        1 gcatgcctgc aggtcgacCC TGGGTTTGAG CAATTCTGCC TGAGCCTCCC AAGTGGCTGG
       61 GATTACAGGT GCACACCATC ACACCTGGCT AATTTTTGTA TTTTCAGTAG AGACGGGGTT
      121 TCACCATGTT GGCCAGGCTG GTCTTGAACT CCTGGCCTCA AGTGATTTGC CCACCTCGGC
      181 CTCCCACAGT GTTGGGTTTA CAGGTGTGAG CCACCTTGTC CAGCCCTACT TCCTGCCTCC
      241 TGAGCACTGG GTAGTATTTC ACAGTGTGGA AATAGCATGG CTTATGTATC CAGCTGTATG
      301 TTGGTGGACA TTTGGGTTGT CTGACTTTTG GTATTTTAGG CGATGACACA CATCCCTTTC
      361 TGAAGGGCCT TTGGGTGGTC TCTGTTCTTT GACCACATAC ACAATACTGC AGGTCTTTGT
      421 ACTTGGTGGG ATGTCTCTGC AGACCGAGTT CCTAGACAGG GCAAGGTGAG TGGTTGGTCT
      481 GTCTTCCGGG CTTGGTTGTG GTCTGATTCT GACCCTGTTA CCTTATTCCA CAGGGGACCA
      541 ACAAGGCACC ATGGCGttgg atccaatggt cttcacactc gaagatttcg ttggggactg
      601 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
      661 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
      721 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
      781 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
      841 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
      901 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
      961 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     1021 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgGG
     1081 AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG
     1141 ACCTacccgg ggggtaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
     1201 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1261 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1321 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1381 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1441 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1501 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1561 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     1621 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     1681 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     1741 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     1801 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     1861 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     1921 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     1981 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     2041 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     2101 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     2161 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     2221 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     2281 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     2341 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     2401 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     2461 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     2521 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     2581 ggacgagctg tacaagtagg cggccgcgac tctagatcat aatcagccat accacatttg
     2641 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2701 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2761 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2821 ccaaactcat caatgtatct taaggcgaga tcttgaagtt cctatacttt ctagagaata
     2881 ggaacttcac gaagttatat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     2941 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     3001 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccgtcgc
     3061 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     3121 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     3181 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     3241 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     3301 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     3361 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcagcac
     3421 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     3481 actaaaccat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     3541 gggtggagag gctattcggc tatgactggg cacaacagac gatcggctgc tctgatgccg
     3601 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3661 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3721 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3781 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3841 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3901 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3961 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4021 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4081 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4141 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4201 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc ccccgattcg cagcgcatcg
     4261 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aataaagacc
     4321 gaccaagcga cgtctgagag ctccctggcg aattcggtac caataaaaga gctttatttt
     4381 catgatctgt gtgttggttt ttgtgtgcgg cgcgataact tcgtatagct gaagttccta
     4441 tactttctag agaataggaa cttcgctagc ATCAACAGCC CAGTGGCCCA GGCTGACTGG
     4501 GCCGCCAAGA GACTCGTCTG GGTCCCCTCG GAGAAGCAGG GCTTCGAGGC AGCCAGCATT
     4561 AAGGAGGAGA AGGGGGATGA GGTGGTTGTG GAGCTGGTGG AGAATGGCAA GAAGGTCACG
     4621 GTTGGGAAAG ATGACATCCA GAAGATGAAC CCACCCAAGT TCTCCAAGGT GGAGGACATG
     4681 GCGGAGCTGA CGTGCCTCAA CGAAGCCTCC GTGCTACACA ACCTGAGGGA GCGGTACTTC
     4741 TCAGGGCTAA TATATGTGAG TATTGCAGGC AGCCAGGTAC CTACTCCTGG CCTCATAAGG
     4801 AAAGTAGGCA TTAGAAATGT GTTGGGAGGG CTGGGCACAG TGGCTCATGC CTGTACTCCC
     4861 AGCACTTTGG GAGGCCGAGG TGGGCAGATC ACCTGAGGTC AGAAGTTCGA GACCACGCTG
     4921 GCCAACATGA TGAAATGCCG TCTCTACTAA AAATACAAAA AGTAGCCAAG GGTGGTGGTG
     4981 CGCATCTGTA ATCCCAGCTA CTGGGGAGGC TGGGGCAGAG GAATTGCTTG AATCCAGGAG
     5041 GTGGAGGTGC AGTGAGCCAA GACTGTACCA CTGCACTCCA GCCTGGGTGA CAGAATAAGA
     5101 CTCTGTCTCA AAAAAAAAAA AAAAGGAAAT GGGAGGCTGa cgcgtaaggc ggtaatacgg
     5161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt caatgccaat
     7021 aggatatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc cttgttcaag
     7081 gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga agctaggcgc
     7141 aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg taatcacgac
     7201 attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta catcgttagg
     7261 atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg ggccagttaa
     7321 agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt caatcgtcat
     7381 ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa agacgttcgc
     7441 gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca cttttttcag
     7501 tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact cagccgtgcg
     7561 ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg tgcttttgcc
     7621 atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag ttccagtgtt
     7681 tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat ctctcagcgt
     7741 atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat tttgatacgt
     7801 ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt tcaaagagct
     7861 gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt aatgtttacc
     7921 ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg ctgaacctga
     7981 ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt cgctgtcttt
     8041 aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt tttgatagaa
     8101 catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa agacgatgtg
     8161 gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc tgtcccaaac
     8221 gtccaggcct tttgcagaag agatattttt aattgtggac gaatcgaact caggaacttg
     8281 atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg taatatggga
     8341 aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg cttgagttgc
     8401 gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg caaacttttt
     8461 gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc ttcttccagc
     8521 cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta aaatatgtaa
     8581 ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc ctgctttatc
     8641 agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc gtttggattg
     8701 caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt gcagactacg
     8761 ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt ttatagtttc
     8821 tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca taatatctca
     8881 tttcactaaa taatagtgaa cggcaggtat atgtggacgt cgcggccgca taacttcgta
     8941 taatgtatgc tatacgaagt tatggatctt tcaggagcta aggaagctaa aatggagaaa
     9001 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     9061 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     9121 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     9181 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     9241 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     9301 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     9361 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     9421 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     9481 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     9541 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     9601 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc
     9661 gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     9721 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gcactagtca
     9781 tgaccaggat ctcctaggct agttgtgatt atgattatga ttatgattat gattatgacc
     9841 acttacctgg gcccgcgcgc caaacgaatg gtctagaaag ctt
//

