LOCUS       RSF1010_Y25F_bom        8285 bp ds-DNA     circular     30-OCT-2019
DEFINITION  Broad host range vector vector pCVD047, complete sequence.
ACCESSION   KM017898
VERSION     KM017898.1
KEYWORDS    .
SOURCE      Broad host range vector vector pCVD047
  ORGANISM  Broad host range vector vector pCVD047 other sequences; artificial
            sequences; vectors.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Taton,A., Unglaub,F., Wright,N.E., Zeng,W.Y., Paz-Yepez,J.,
            Brahamsha,B., Palenik,B., Peterson,T.C., Haerizadeh,F., Golden,S.S.
            and Golden,J.W.
  TITLE     Broad-host-range vector system for synthetic biology and
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
   PUBMED   25074377
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Taton,A., Unglaub,F., Wright,N., Zeng,W.Y., Paz Yepes,J.,
            Brahamsha,B., Palenik,B., Peterson,T., Haerizadeh,F., Golden,S.S.
            and Golden,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     GC-adaptor      8265..8285
                     /id="CVP002"
                     /note="GC-adaptor for seamless assembly, flanking
                     functional devices, custom sequences or cyanobacterial
                     replicons."
                     /label=G5C5
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     GC-adaptor      6101..6121
                     /id="CVP005"
                     /note="GC-adaptor for seamless assembly, flanking  BHR
                     replicons or neutral sites."
                     /label=GC
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     GC-adaptor      7356..7376
                     /id="CVP006"
                     /note="GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers, functional devices or
                     custom sequences."
                     /label=C2G
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_mob         4913..5197
                     /id="CVP033"
                     /note="Mobilization gene - from pRL1383a (AF403426) , a
                     derivative of RSF1010 (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=mobC
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_mob         complement(3553..3966)
                     /id="CVP034"
                     /note="Mobilization gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=mobB
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_mob         complement(2585..4714)
                     /id="CVP036"
                     /note="Mobilization gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829), point mutation Y25F to reduce the
                     plasmid mobility by more than 99%."
                     /note="Scholz et al. (1989) Gene 75:271-288, Monzingo et
                     al. (2007) J Mol Biol 366:165-78"
                     /label=mobA_Y25F
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_repl        complement(2104..2310)
                     /id="CVP037"
                     /note="repF-RSF1010 - replication gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=repF
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_repl        complement(2312..2524)
                     /id="CVP038"
                     /note="repE-RSF1010 - replication gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=repE
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_repl        complement(1235..2074)
                     /id="CVP042"
                     /note="repA-RSF1010 - replication gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=repA
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_repl        complement(397..1248)
                     /id="CVP043"
                     /note="repC-RSF1010 - replication gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=repC
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_repl        complement(2585..3556)
                     /id="CVP044"
                     /note="repA-RSF1010 - replication gene - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829)."
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=repB
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_reporter    7454..8173
                     /id="CVP054"
                     /note="Genetic mutant of green fluorescent protein,
                     derived from Aequorea victoria. Its excitation peak is
                     514?nm and its emission peak is 527?nm."
                     /note="Dong et al. (2009) Cell 140:529-539"
                     /label=yfp
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ori_rep         complement(5130..5629)
                     /id="CVP057"
                     /note="Origin of replication - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829). "
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=oriV
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ori_tra         4795..4882
                     /id="CVP060"
                     /note="Origin of transfer from conjugation - from pRL1383a
                     (GenBank:AF403426) , a derivative of RSF1010
                     (GenBank:M28829). "
                     /note="Scholz et al. (1989) Gene 75:271-288"
                     /label=oriT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter_antR   complement(6960..7343)
                     /id="CVP079"
                     /note="Upstream region of aadA ORF incl. promoter and RBS
                     - from pRL1383a (AF403426), originally from the omega
                     interposon (GenBank: M60473)."
                     /note="Prentki et al. (1991) Gene 103:17-23"
                     /label=PaadA
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter_repl   complement(2554..2581)
                     /id="CVP080"
                     /note="From pRL1383a (GenBank:AF403426) , a derivative of
                     RSF1010 (GenBank:M28829). The P4 promoter is under the
                     auto-regulated control of the F repressor, whose gene is
                     located in the same operon, providing transcription of
                     (E-F-repA-repC)-operon."
                     /note="Scholz et al. (1989) Gene 75:271-288 / Katashkina
                     et al. (1997) BMC Biotechnology 7:80"
                     /label=P4
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter_repl   complement(4872..4902)
                     /id="CVP081"
                     /note="From pRL1383a (GenBank:AF403426) , a derivative of
                     RSF1010 (GenBank:M28829). The P3 is controlled by MobC and
                     MobA provide transcription of mobA/repB, mobB, and,
                     probably, (E-F-repA-repC)-operon."
                     /note="Scholz et al. (1989) Gene 75:271-288 / Katashkina
                     et al. (1997) BMC Biotechnology 7:80"
                     /label=P3
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter_repl   complement(4891..4922)
                     /id="CVP082"
                     /note="From pRL1383a (GenBank:AF403426) , a derivative of
                     RSF1010 (GenBank:M28829). The P1 promoter is controlled by
                     MobC and MobA provide transcription of mobA/repB, mobB,
                     and, probably, (E-F-repA-repC)-operon."
                     /note="Scholz et al. (1989) Gene 75:271-288 / Katashkina
                     et al. (1997) BMC Biotechnology 7:80"
                     /label=P1
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter_repl   4849..4880
                     /id="CVP083"
                     /note="From pRL1383a (GenBank:AF403426) , a derivative of
                     RSF1010 (GenBank:M28829). The P2 promoter is controlled by
                     MobC and MobA provide transcription of mobC."
                     /note="Scholz et al. (1989) Gene 75:271-288 / Katashkina
                     et al. (1997) BMC Biotechnology 7:80"
                     /label=P2
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      complement(6137..6163)
                     /id="CVP098"
                     /note="trpA terminator from Lorist6 (GenBank: X98450), one
                     nt shorter than reference."
                     /note="Gibson et al. (1987) Gene 53:283-286"
                     /label=trpA T
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      38..64
                     /id="CVP098-1"
                     /note="trpA terminator from Lorist6 (GenBank: X98450), one
                     nt shorter than reference."
                     /note="Gibson et al. (1987) Gene 53:283-286"
                     /label=trpA T(1)
                     /ApEinfo_label="trpA T"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      8223..8251
                     /id="CVP100"
                     /note="rrnB T2 - rrnB T2 terminator (partial sequence)."
                     /note="Orosz et al. (1991) Eur. J. Biochem. 201:653-659"
                     /label=rrnB T2
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      complement(5790..5818)
                     /id="CVP101"
                     /note="rrnC terminator from Lorist6 (GenBank: X98450)."
                     /note="Gibson et al. (1987) Gene 53:283-286"
                     /label=TrrnC
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ORF_antR        complement(6169..6959)
                     /id="CVP109"
                     /note="Confers spectinomycin and/or streptomycin
                     resistance - aminoglycoside-3:adenyltransferase fusion -
                     from pRL1383a (AF403426), originally from the omega
                     interposon (GenBank: M60473). NOTE: The stop codon was
                     erased by mistake however as part of the assembly device
                     CVD002/CVD-aadA, a stop codon is loacted 15 nucleotides
                     downstream."
                     /note="Prentki et al. (1991) Gene 103:17-23"
                     /label=aadA
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             7439..7453
                     /id="CVP122"
                     /note="RBS and flanking sequence with AflII site."
                     /label=RBS_02
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        7395..7426
                     /id="CVP126"
                     /label=PconII
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5858..6099
                     /label=RK2BOM
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5
                     offset 0"
ORIGIN
        1 TCTAGAACAA TGGAAGTCCT CGTCTCGCCC TCGAATTAGC CCGCTTAATG AGCGGGCTTT
       61 TTTTGAATTA ATTCTCGCGA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
      121 GCGCAACGCA ATTAATGTAA GTTAGCGCGA ATTGCAAGCT GGCCGACGCG CTGGGCTACG
      181 TCTTGCTGGC GTTCGGGAGC AGAAGAGCAT ACATCTGGAA GCAAAGCCAG GAAAGCGGCC
      241 TATGGAGCTG TGCGGCAGCG CTCAGTAGGC AATTTTTCAA AATATTGTTA AGCCTTTTCT
      301 GAGCATGGTA TTTTTCATGG TATTACCAAT TAGCAGGAAA ATAAGCCATT GAATATAAAA
      361 GATAAAAATG TCTTGTTTAC AATAGAGTGG GGGGGGTCAG CCTGCCGCCT TGGGCCGGGT
      421 GATGTCGTAC TTGCCCGCCG CGAACTCGGT TACCGTCCAG CCCAGCGCGA CCAGCTCCGG
      481 CAACGCCTCG CGCACCCGCT GGCGGCGCTT GCGCATGGTC GAACCACTGG CCTCTGACGG
      541 CCAGACATAG CCGCACAAGG TATCTATGGA AGCCTTGCCG GTTTTGCCGG GGTCGATCCA
      601 GCCACACAGC CGCTGGTGCA GCAGGCGGGC GGTTTCGCTG TCCAGCGCCC GCACCTCGTC
      661 CATGCTGATG CGCACATGCT GGCCGCCACC CATGACGGCC TGCGCGATCA AGGGGTTCAG
      721 GGCCACGTAC AGGCGCCCGT CCGCCTCGTC GCTGGCGTAC TCCGACAGCA GCCGAAACCC
      781 CTGCCGCTTG CGGCCATTCT GGGCGATGAT GGATACCTTC CAAAGGCGCT CGATGCAGTC
      841 CTGTATGTGC TTGAGCGCCC CACCACTATC GACCTCTGCC CCGATTTCCT TTGCCAGCGC
      901 CCGATAGCTA CCTTTGACCA CCATGGCATC AGCGGTGACG GCCTCCCACT TGGGTTCCAG
      961 GAACAGCCGG AGCTGCCGTC CGCCTTCGGT CTTGGGTTCC GGGCCAAGCA CTAGGCCATT
     1021 AGGCCCAGCC ATGGCCACCA GCCCTTGCAG GATGCGCAGA TCATCAGCGC CCAGCGGCTC
     1081 CGGGCCGCTG AACTCGATCC GCTTGCCGTC GCCGTAGTCA TACGTCACGT CCAGCTTGCT
     1141 GCGCTTGCGC TCGCCCCGCT TGAGGGCACG GAACAGGCCG GGGGCCAGAC AGTGCGCCGG
     1201 GTCGTGTCGG ACGTGGCTGA GGCTGTGCTT GTTCTTAGGC TTCACCACGG GGCACCCCCT
     1261 TGCTCTTGCG CTGCCTCTCC AGCACGGCGG GCTTGAGCAC CCCGCCGTCA TGCCGCCTGA
     1321 ACCACCGATC AGCGAACGGT GCGCCATAGT TGGCCTTGCT CACACCGAAG CGGACGAAGA
     1381 ACCGGCGCTG GTCGTCGTCC ACACCCCATT CCTCGGCCTC GGCGCTGGTC ATGCTCGACA
     1441 GGTAGGACTG CCAGCGGATG TTATCGACCA GTACCGAGCT GCCCCGGCTG GCCTGCTGCT
     1501 GGTCGCCTGC GCCCATCATG GCCGCGCCCT TGCTGGCATG GTGCAGGAAC ACGATAGAGC
     1561 ACCCGGTATC GGCGGCGATG GCCTCCATGC GACCGATGAC CTGGGCCATG GGGCCGCTGG
     1621 CGTTTTCTTC CTCGATGTGG AACCGGCGCA GCGTGTCCAG CACCATCAGG CGGCGGCCCT
     1681 CGGCGGCGCG CTTGAGGCCG TCGAACCACT CCGGGGCCAT GATGTTGGGC AGGCTGCCGA
     1741 TCAGCGGCTG GATCAGCAGG CCGTCAGCCA CGGCTTGCCG TTCCTCGGCG CTGAGGTGCG
     1801 CCCCAAGGGC GTGCAGGCGG TGATGAATGG CGGTGGGCGG GTCTTCGGCG GGCAGGTAGA
     1861 TCACCGGGCC GGTGGGCAGT TCGCCCACCT CCAGCAGATC CGGCCCGCCT GCAATCTGTG
     1921 CGGCCAGTTG CAGGGCCAGC ATGGATTTAC CGGCACCACC GGGCGACACC AGCGCCCCGA
     1981 CCGTACCGGC CACCATGTTG GGCAAAACGT AGTCCAGCGG TGGCGGCGCT GCTGCGAACG
     2041 CCTCCAGAAT ATTGATAGGC TTATGGGTAG CCATTGATTG CCTCCTTTGC AGGCAGTTGG
     2101 TGGTTAGGCG CTGGCGGGGT CACTACCCCC GCCCTGCGCC GCTCTGAGTT CTTCCAGGCA
     2161 CTCGCGCAGC GCCTCGTATT CGTCGTCGGT CAGCCAGAAC TTGCGCTGAC GCATCCCTTT
     2221 GGCCTTCATG CGCTCGGCAT ATCGCGCTTG GCGTACAGCG TCAGGGCTGG CCAGCAGGTC
     2281 GCCGGTCTGC TTGTCCTTTT GGTCTTTCAT ATCAGTCACC GAGAAACTTG CCGGGGCCGA
     2341 AAGGCTTGTC TTCGCGGAAC AAGGACAAGG TGCAGCCGTC AAGGTTAAGG CTGGCCATAT
     2401 CAGCGACTGA AAAGCGGCCA GCCTCGGCCT TGTTTGACGT ATAACCAAAG CCACCGGGCA
     2461 ACCAATAGCC CTTGTCACTT TTGATCAGGT AGACCGACCC TGAAGCGCTT TTTTCGTATT
     2521 CCATAAAACC CCCTTCTGTG CGTGAGTACT CATAGTATAA CAGGCGTGAG TACCAACGCA
     2581 AGCACTACAT GCTGAAATCT GGCCCGCCCC TGTCCATGCC TCGCTGGCGG GGTGCCGGTG
     2641 CCCGTGCCAG CTCGGCCCGC GCAAGCTGGA CGCTGGGCAG ACCCATGACC TTGCTGACGG
     2701 TGCGCTCGAT GTAATCCGCT TCGTGGCCGG GCTTGCGCTC TGCCAGCGCT GGGCTGGCCT
     2761 CGGCCATGGC CTTGCCGATT TCCTCGGCAC TGCGGCCCCG GCTGGCCAGC TTCTGCGCGG
     2821 CGATAAAGTC GCACTTGCTG AGGTCATCAC CGAAGCGCTT GACCAGCCCG GCCATCTCGC
     2881 TGCGGTACTC GTCCAGCGCC GTGCGCCGGT GGCGGCTAAG CTGCCGCTCG GACAGTTCGA
     2941 GGCTGGCCAG CCTGCGGGCC TTCTCCTGCT GCCGCTGGGC CTGCTCGATC TGCTGGCCAG
     3001 CCTGCTGCAC CAGCGCCGGG CCAGCGGTGG CGGTCTTGCC CTTGGATTCA CGCAGCAGCA
     3061 CCCACGGCTG ATAACCGGCG CGGGTGGTGT GCTTGTCCTT GCGGTTGGTG AAGCCCGCCA
     3121 AGCGGCCATA GTGGCGGCTG TCGGCGCTGG CCGGGTCGGC GTCGTACTCG CTGGCCAGCG
     3181 TCCGGGCAAT CTGCCCCCGA AGTTCACCGC CTGCGGCGTC GGCCACCTTG ACCCATGCCT
     3241 GATAGTTCTT CGGGCTGGTT TCCACTACCA GGGCAGGCTC CCGGCCCTCG GCTTTCATGT
     3301 CATCCAGGTC AAACTCGCTG AGGTCGTCCA CCAGCACCAG ACCATGCCGC TCCTGCTCGG
     3361 CGGGCCTGAT ATACACGTCA TTGCCCTGGG CATTCATCCG CTTGAGCCAT GGCGTGTTCT
     3421 GGAGCACTTC GGCGGCTGAC CATTCCCGGT TCATCATCTG GCCGGTGGTG GCGTCCCTGA
     3481 CGCCGATATC GAAGCGCTCA CAGCCCATGG CCTTGAGCTG TCGGCCTATG GCCTGCAAAG
     3541 TCCTGTCGTT CTTCATCGGG CCACCAAGCG CAGCCAGATC GAGCCGTCCT CGGTTGTCAG
     3601 TGGCGTCAGG TCGAGCAAGA GCAACGATGC GATCAGCAGC ACCACCGTAG GCATCATGGA
     3661 AGCCAGCATC ACGGTTAGCC ATAGCTTCCA GTGCCACCCC CGCGACGCGC TCCGGGCGCT
     3721 CTGCGCGGCG CTGCTCACCT CGGCGGCTAC CTCCCGCAAC TCTTTGGCCA GCTCCACCCA
     3781 TGCCGCCCCT GTCTGGCGCT GGGCTTTCAG CCACTCCGCC GCCTGCGCCT CGCTGGCCTG
     3841 CTGGGTCTGG CTCATGACCT GCCGGGCTTC GTCGGCCAGT GTCGCCATGC TCTGGGCCAG
     3901 CGGTTCGATC TGCTCCGCTA ACTCGTTGAT GCCTCTGGAT TTCTTCACTC TGTCGATTGC
     3961 GTTCATGGTC TATTGCCTCC CGGTATTCCT GTAAGTCGAT GATCTGGGCG TTGGCGGTGT
     4021 CGATGTTCAG GGCCACGTCT GCCCGGTCGG TGCGGATGCC CCGGCCTTCC ATCTCCACCA
     4081 CGTTCGGCCC CAGGTGAACA CCGGGCAGGC GCTCGATGCC CTGCGCCTCA AGTGTTCTGT
     4141 GGTCAATGCG GGCGTCGTGG CCTGCCCGCT CTAATGCCCG GTTGGCATGG TCGGCCCATG
     4201 CCTCGCGGGT CTGCTCAAGC CATGCCTTGG GCTTGAGCGC TTCGGTCTTC TGTGCCCCGC
     4261 CCTTCTCCGG GGTCTTGCCG TTGTACCGCT TGAACCACTG AGCGGCGGGC CGCTCGATGC
     4321 CGTCATTGAT CCGCTCGGAG ATCATCAGGT GGCAGTGCGG GTTCTCGCCG CCACCGGCAT
     4381 GGATGGCCAG CGTATACGGC AGGCGCTCGG CACCGGTCAG GTGCTGGGCG AACTCGGACG
     4441 CCAGCGCCTT CTGCTGGTCG AGGGTCAGCT CGACCGGCAG GGCAAATTCG ACCTCCTTGA
     4501 ACAGCCGCCC ATTGGCGCGT TCATACAGGT CGGCAGCATC CCAGTAGTCG GCGGGCCGCT
     4561 CGACGAACTC CGGCATGTGC CCGGATTCGG CGTGCAAGAC TTCATCCATG TCGCGGGCAT
     4621 ACTTGCCTTC GCGCTGGATG AAGTCGGCCT TGGCCCTGGC CGATTGGCCG CCCGACCTGC
     4681 TGCCGGTTTT CGCCGTAAGG TGATAAATCG CCATGCTGCC TCGCTGTTGC TTTTGCTTTT
     4741 CGGCTCCATG CAATGGCCCT CGGAGAGCGC ACCGCCCGAA GGGTGGCCGT TAGGCCAGTT
     4801 TCTCGAAGAG AAACCGGTAA GTGCGCCCTC CCCTACAAAG TAGGGTCGGG ATTGCCGCCG
     4861 CTGTGCCTCC ATGATAGCCT ACGAGACAGC ACATTAACAA TGGGGTGTCA AGATGGTTAA
     4921 GGGGAGCAAC AAGGCGGCGG ATCGGCTGGC CAAGCTCGAA GAACAACGAG CGCGAATCAA
     4981 TGCCGAAATT CAGCGGGTGC GGGCAAGGGA ACAGCAGCAA GAGCGCAAGA ACGAAACAAG
     5041 GCGCAAGGTG CTGGTGGGGG CCATGATTTT GGCCAAGGTG AACAGCAGCG AGTGGCCGGA
     5101 GGATCGGCTC ATGGCGGCAA TGGATGCGTA CCTTGAACGC GACCACGACC GCGCCTTGTT
     5161 CGGTCTGCCG CCACGCCAGA AGGATGAGCC GGGCTGAATG ATCGACCGAG ACAGGCCCTG
     5221 CGGGGCTGCA CACGCGCCCC CACCCTTCGG GTAGGGGGAA AGGCCGCTAA AGCGGCTAAA
     5281 AGCGCTCCAG CGTATTTCTG CGGGGTTTGG TGTGGGGTTT AGCGGGCTTT GCCCGCCTTT
     5341 CCCCCTGCCG CGCAGCGGTG GGGCGGTGTG TAGCCTAGCG CAGCGAATAG ACCAGCTATC
     5401 CGGCCTCTGG CCGGGCATAT TGGGCAAGGG CAGCAGCGCC CCACAAGGGC GCTGATAACC
     5461 GCGCCTAGTG GATTATTCTT AGATAATCAT GGATGGATTT TTCCAACACC CCGCCAGCCC
     5521 CCGCCCCTGC TGGGTTTGCA GGTTTGGGGG CGTGACAGTT ATTGCAGGGG TTCGTGACAG
     5581 TTATTGCAGG GGGGCGTGAC AGTTATTGCA GGGGTTCGTG ACAGTTAGTA CGGGAGTGAC
     5641 GGGCACTGGC TGGCAATGTC TAGCAACGGC AGGCATTTCG GCTGAGGGTA AAAGAACTTT
     5701 CCGCTAAGCG ATAGACTGTA TGTAAACACA GTATTGCAAG GACGCGGAAC ATGCCTCATG
     5761 TGGCGGCCCT CGCGAGAATT AATTCAGATA AAAAAAATCC TTAGCTTTCG CTAAGGATGA
     5821 TTTCTAGCGA TGACCCTGCT GATTGGTTCG CTGACCGTAG ACTGGTTGGC TTGGTTTCAT
     5881 CAGCCATCCG CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT CGCAGAGCAG
     5941 GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG
     6001 CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT
     6061 CTACaCGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAC ATCGCGCGCG CGCGCGCGGA
     6121 TGCTAGCGGC GCGCCAAAAA AAAGCCCGCT CATTAGGCGG GCTGGCCGGC CTTGCCGACT
     6181 ACCTTGGTGA TCTCGCCTTT CACGTAGTGG ACAAATTCTT CCAACTGATC TGCGCGCGAG
     6241 GCCAAGCGAT CTTCTTCTTG TCCAAGATAA GCCTGTCTAG CTTCAAGTAT GACGGGCTGA
     6301 TACTGGGCCG GCAGGCGCTC CATTGCCCAG TCGGCAGCGA CATCCTTCGG CGCGATTTTG
     6361 CCGGTTACTG CGCTGTACCA AATGCGGGAC AACGTAAGCA CTACATTTCG CTCATCGCCA
     6421 GCCCAGTCGG GCGGCGAGTT CCATAGCGTT AAGGTTTCAT TTAGCGCCTC AAATAGATCC
     6481 TGTTCAGGAA CCGGATCAAA GAGTTCCTCC GCCGCTGGAC CTACCAAGGC AACGCTATGT
     6541 TCTCTTGCTT TTGTCAGCAA GATAGCCAGA TCAATGTCGA TCGTGGCTGG CTCGAAGATA
     6601 CCTGCAAGAA TGTCATTGCG CTGCCATTCT CCAAATTGCA GTTCGCGCTT AGCTGGATAA
     6661 CGCCACGGAA TGATGTCGTC GTGCACAACA ATGGTGACTT CTACAGCGCG GAGAATCTCG
     6721 CTCTCTCCAG GGGAAGCCGA AGTTTCCAAA AGGTCGTTGA TCAAAGCTCG CCGCGTTGTT
     6781 TCATCAAGCC TTACGGTCAC CGTAACCAGC AAATCAATAT CACTGTGTGG CTTCAGGCCG
     6841 CCATCCACTG CGGAGCCGTA CAAATGTACG GCCAGCAACG TCGGTTCGAG ATGGCGCTCG
     6901 ATGACGCCAA CTACCTCTGA TAGTTGAGTC GATACTTCGG CGATCACCGC TTCCCTCATG
     6961 ATGTTTAACT TTGTTTTAGG GCGACTGCCC TGCTGCGTAA CATCGTTGCT GCTCCATAAC
     7021 ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG ATGCCCGAGG CATAGACTGT
     7081 ACCCCAAAAA AACAGTCATA ACAAGCCATG AAAACCGCCA CTGCGCCGTT ACCACCGCTG
     7141 CGTTCGGTCA AGGTTCTGGA CCAGTTGCGT GAGCGCATAC GCTACTTGCA TTACAGCTTA
     7201 CGAACCGAAC AGGCTTATGT CCACTGGGTT CGTGCCTTCA TCCGTTTCCA CGGTGTGCGT
     7261 CACCCGGCAA CCTTGGGCAG CAGCGAAGTC GAGGCATTTC TGTCCTGGCT GGCGAACGAG
     7321 CGCAAGGTTT CGGTCTCCAC GCATTCGAAA CCGGTATCCC GCCGCCGCCG CCGGATACCG
     7381 GTTTCGAATT GAGATTGACA ATTAATCATC GGCTCGTATA ATGGTACCCT ATTTAAATAA
     7441 AGGAGGTCTT AAGATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT
     7501 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG
     7561 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
     7621 GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG CAGTGCTTCG CCCGCTACCC
     7681 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA
     7741 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAGGTTCGA
     7801 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA
     7861 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA
     7921 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG
     7981 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
     8041 GCCCGACAAC CACTACCTGA GCTACCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
     8101 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
     8161 GCTGTACAAG TAAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAACT CGGCCCTGCA
     8221 GGAGAAGGCC ATCCTGACGG ATGGCCTTTT TGAAGCTTTC TAGAATCGGG GGCCCCCGGG
     8281 GGGAT     
//
