LOCUS       pRDV5_Sb_convex      3817 bp    DNA     circular UNA 13-Jan-2014
DEFINITION  -tag/-stop/-stemloop
FEATURES             Location/Qualifiers
     promoter        19..35
                     /label="T7"
     CDS             85..90
                     /label="ribosome binding site"
     Site            97..102
                     /site_type="restriction site"
                     /label="NcoI"
     CDS             105..482
                     /label="Sb_convex"
     CDS             489..512
                     /label="linker coming from beta-lactamase"
     CDS             513..1400
                     /label="TolA spacer"
     CDS             complement(754..777)
                     /label="RT_rev_1"
     gene            2138..2998
                     /label="AmpR"
ORIGIN
        1 acccgacacc atcgaaatta atacgactca ctatagggAG ACCACAACGG TTTCCCTCta
       61 gaaataattt tgtttaactt taagaaggag atatatCCAT GGGTAGTCAA GTCCAGCTGG
      121 TGGAATCGGG TGGTGGTAGC GTCCAGGCGG GTGGTAGCCT GCGTCTGAGC TGTGCGGCTA
      181 GCGGCTCTAT TTCCAGCATC ACGTACCTGG GCTGGTTTCG CCAGGCACCG GGCAAAGAGC
      241 GTGAGGGCGT CGCAGCGCTG AGCACCAGCT CCGGTACCAC CTACTACGCG GACAGCGTTA
      301 AGGGTCGTTT CACGGTGAGC CTGGACAACG CCAAGAATAC CGTGTATCTG CAAATGAACA
      361 GCTTGAAACC GGAAGATACT GCTTTGTATT ACTGCGCGGC AGCCAGCAGC GGCTCCAGCA
      421 GCCCGCTGTC TAGCAGCAGC TATACGTACT GGGGTCAGGG CACCCAAGTT ACCGTTTCTG
      481 CAAAGCTTta tatggcctcg ggggccgaat tcggatctgg tggccagaag caagctgaag
      541 aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcagat gctaaagctg
      601 cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa aaaagcagaa gcagaagccg
      661 ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc cgctgcggca ctgaagaaga
      721 aagcggaagc ggcagaagca gctgcagctg aagcaagaaa gaaagcggca actgaagctg
      781 ctgaaaaagc caaagcagaa gctgagaaga aagcggctgc tgaaaaggct gcagctgata
      841 agaaagcggc agcagagaaa gctgcagccg acaaaaaagc agcagaaaaa gcggctgctg
      901 aaaaggcagc agctgataag aaagcagcgg cagaaaaagc cgccgcagac aaaaaagcgg
      961 cagcggcaaa agctgcagct gaaaaagccg ctgcagcaaa agcggccgca gaggcagatg
     1021 atattttcgg tgagctaagc tctggtaaga atgcaccgaa aacgggggga ggggcgaaag
     1081 ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa caatggcgca tcaggggccg
     1141 atatcaataa ctatgccggg cagattaaat ctgctatcga aagtaagttc tatgacgcat
     1201 cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact ggcacccgat ggtatgttac
     1261 tggatatcaa acctgaaggt ggcgatcccg cactttgtca ggctgcgttg gcagcagcta
     1321 aacttgcgaa gatcccgaaa ccaccaagcc aggcagtata tgaagtgttc aaaaacgcgc
     1381 cattggactt caaaccgtag tagagatccg gctgctaaca aagcccgaaa ggaagctgag
     1441 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1501 ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcccc acgcgccctg
     1561 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1621 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1681 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     1741 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1801 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1861 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1921 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1981 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     2041 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2101 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2161 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2221 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2281 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2341 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2401 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2461 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2521 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2581 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2641 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2701 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2761 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2821 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2881 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2941 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3001 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3061 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3301 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3721 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3781 acggttcctg gccttttgct ggccttttgc tcacatg
//
