LOCUS       pYZ_NAND2_L17_S19_S11   4281 bp ds-DNA     circular     27-AUG-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature               4..166
                                /label="NAND2_L17_S19_S11"
                                /ApEinfo_revcolor=#faac61
                                /ApEinfo_fwdcolor=#faac61
     misc_feature               2212..2847
                                /label="ColA ori"
                                /ApEinfo_revcolor=#84b0dc
                                /ApEinfo_fwdcolor=#84b0dc
     misc_feature               complement(2144..2172)
                                /label="AmpR_promoter"
                                /ApEinfo_revcolor=#b4abac
                                /ApEinfo_fwdcolor=#b4abac
     protein                    188..946
                                /label="GFP-ASV"
                                /ApEinfo_revcolor=#75c6a9
                                /ApEinfo_fwdcolor=#75c6a9
     primer_bind                join(4265..4281,1..3)
                                /label="T7"
                                /ApEinfo_revcolor=#85dae9
                                /ApEinfo_fwdcolor=#85dae9
     CDS                        complement(3048..4127)
                                /label="LacI"
                                /ApEinfo_revcolor=#9eafd2
                                /ApEinfo_fwdcolor=#9eafd2
     terminator                 1011..1057
                                /label="T7 terminator"
                                /ApEinfo_revcolor=#c6c9d1
                                /ApEinfo_fwdcolor=#c6c9d1
     antibiotic resistance gene complement(1287..2102)
                                /label="Kan R"
                                /ApEinfo_revcolor=#c7b0e3
                                /ApEinfo_fwdcolor=#c7b0e3
     misc_feature               167..187
                                /label="Linker21"
                                /ApEinfo_revcolor=#c6c9d1
                                /ApEinfo_fwdcolor=#c6c9d1
     promoter                   complement(4128..4205)
                                /label="LacI promoter"
                                /ApEinfo_revcolor=#ff9ccd
                                /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 GGGACTAATC AGATCTACTT GTTATAGTTA TGAACAGAGG AGACATAACA TGAACAAGCA
       61 CCTAACAAGA CTAATCATAT ACAATACCTC CAAATCAATC AATTCTACTT GTTATAGTTA
      121 TGAACAGAGG AGACATAACA TGAACAATCA CATAAACCTA AGAACTAACC TGGCGGCAGC
      181 GCAAAAGATG CGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT
      241 AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC
      301 ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC
      361 AACACTTGTC ACTACTTTCG GTTATGGTGT TCAATGCTTT GCGAGATACC CAGATCACAT
      421 GAAACAGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TACGTACAGG AAAGAACTAT
      481 ATTTTTCAAA GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC
      541 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG
      601 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA
      661 GAATGGAATC AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT
      721 AGCAGACCAT TATCAACAAA ATACTCCGAT TGGCGATGGC CCTGTCCTTT TACCAGACAA
      781 CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT
      841 GGTCCTTCTT GAGTTTGTAA CCGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA
      901 AAGGCCTGCA GCAAACGACG AAAACTACGC TGCATCAGTT TAATAAGATA AACCAGAGCG
      961 GCACGGCAAG CAGAGTATAC GAGATTCGGT AGCCACCGCT GAGCAATAAC TAGCATAACC
     1021 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAACCTCAGG CATTTGAGAA
     1081 GCACACGGTC ACACTGCTTC CGGTAGTCAA TAAACCGGTA AACCAGCAAT AGACATAAGC
     1141 GGCTATTTAA CGACCCTGCC CTGAACCGAC GACAAGCTGA CGACCGGGTC TCCGCAAGTG
     1201 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     1261 ATATGTATCC GCTCATGAAT TAATTCTTAG AAAAACTCAT CGAGCATCAA ATGAAACTGC
     1321 AATTTATTCA TATCAGGATT ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA
     1381 GGAGAAAACT CACCGAGGCA GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT
     1441 CCGACTCGTC CAACATCAAT ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA
     1501 AGTGAGAAAT CACCATGAGT GACGACTGAA TCCGGTGAGA ATGGCAAAAG TTTATGCATT
     1561 TCTTTCCAGA CTTGTTCAAC AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCATCA
     1621 ACCAAACCGT TATTCATTCG TGATTGCGCC TGAGCGAGAC GAAATACGCG GTCGCTGTTA
     1681 AAAGGACAAT TACAAACAGG AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA
     1741 ACAATATTTT CACCTGAATC AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCGGGG
     1801 ATCGCAGTGG TGAGTAACCA TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA
     1861 AGAGGCATAA ATTCCGTCAG CCAGTTTAGT CTGACCATCT CATCTGTAAC ATCATTGGCA
     1921 ACGCTACCTT TGCCATGTTT CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA
     1981 TAGATTGTCG CACCTGATTG CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA
     2041 GCATCCATGT TGGAATTTAA TCGCGGCCTA GAGCAAGACG TTTCCCGTTG AATATGGCTC
     2101 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     2161 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGCA TGCTAGCGCA GAAACGTCCT
     2221 AGAAGATGCC AGGAGGATAC TTAGCAGAGA GACAATAAGG CCGGAGCGAA GCCGTTTTTC
     2281 CATAGGCTCC GCCCCCCTGA CGAACATCAC GAAATCTGAC GCTCAAATCA GTGGTGGCGA
     2341 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG ATGGCTCCCT CTTGCGCTCT
     2401 CCTGTTCCCG TCCTGCGGCG TCCGTGTTGT GGTGGAGGCT TTACCCAAAT CACCACGTCC
     2461 CGTTCCGTGT AGACAGTTCG CTCCAAGCTG GGCTGTGTGC AAGAACCCCC CGTTCAGCCC
     2521 GACTGCTGCG CCTTATCCGG TAACTATCAT CTTGAGTCCA ACCCGGAAAG ACACGACAAA
     2581 ACGCCACTGG CAGCAGCCAT TGGTAACTGA GAATTAGTGG ATTTAGATAT CGAGAGTCTT
     2641 GAAGTGGTGG CCTAACAGAG GCTACACTGA AAGGACAGTA TTTGGTATCT GCGCTCCACT
     2701 AAAGCCAGTT ACCAGGTTAA GCAGTTCCCC AACTGACTTA ACCTTCGATC AAACCGCCTC
     2761 CCCAGGCGGT TTTTTCGTTT ACAGAGCAGG AGATTACGAC GATCGTAAAA GGATCTCAAG
     2821 AAGATCCTTT ACGGATTCCC GACACCATCA CTCTAGATTT CAGTGCAATT TATCTCTTCA
     2881 AATGTAGCAC CTGAAGTCAG CCCCATACGA TATAAGTTGT AATTCTCATG TTAGTCATGC
     2941 CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC
     3001 CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
     3061 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     3121 GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA
     3181 TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC
     3241 AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG
     3301 GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC GCAGCCCGGA CTCGGTAATG
     3361 GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG
     3421 CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC
     3481 CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC
     3541 AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT
     3601 GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG
     3661 ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC
     3721 ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC
     3781 GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC
     3841 ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC
     3901 GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC
     3961 AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT
     4021 TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA
     4081 GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG
     4141 AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG
     4201 GTGTCCGGGA TCTCGACGCT CTCCCTTATG GCCGGGTTTA GAAATCTGAA GCTCTAGAGA
     4261 GCGCTAATAC GACTCACTAT A
//