LOCUS       Exported                3223 bp ds-DNA     circular SYN 11-JAN-2019
DEFINITION  wolf1_1355713284 in pUCIDT-AMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 11, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="pUCIDT-AMP"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..953
                     /function="Reference Gene"
                     /label=wolf1_1355713284
                     /note="wolf1_1355713284"
     primer_bind     489..507
                     /label=Tet2_for
     misc_feature    508..530
                     /label=Hash_for
     misc_feature    905..927
                     /label=Hash_rev
     primer_bind     complement(928..949)
                     /label=Tet2_rev
     primer_bind     complement(1002..1018)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(1015..1037)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     rep_origin      1389..2003
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(2163..3023)
                     /function="Drug Resistance"
                     /label=Ampicillin
                     /note="Ampicillin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc atctctagcg agctacagca ctcacacacg
      541 atgacgatca tgtacgagct cggcactgat atggagactc tctatctcag gcctctactt
      601 ctacactgag agattatctg gtctttcgcc gcagattagc taagcttaaa aatcgttcaa
      661 cggtaactaa ccctgctttc ttgaggaact ttccgggccg tcccggtaca tcgtacccgt
      721 ttactaccaa cttacaacac atggcgcaga tctaagcatc gagtttcaaa atgccatttc
      781 tatgataccc gctgtactcc caccaaagat acgaaatgcc caccttctaa aagttatctc
      841 cacatataga gcgactacgc agaaggcagg aataactcct atatttaggt tttagctcgg
      901 tttatcgctg acgcgctcag tacagcagct gaaaaggtgg catcaatctg cagatcagtt
      961 ctggaccagc gagctgtgct gcgactcgtg gcgtaatcat ggtcatagct gtttcctgtg
     1021 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1081 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1141 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1201 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgtcgcttac cggatacctg
     1561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1801 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2161 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2221 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2281 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2341 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2401 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2461 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2521 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2581 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2641 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2701 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2761 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2821 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2881 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2941 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3001 acacggaaat gttgaatact catactctac ctttttcaat attattgaag catttatcag
     3061 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3121 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
