LOCUS       Exported                3041 bp ds-DNA     circular SYN 11-JAN-2019
DEFINITION  dog1_647443051 in pUCIDT-AMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 11, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="pUCIDT-AMP"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..771
                     /function="Reference Gene"
                     /label=dog1_647443051
                     /note="dog1_647443051"
     misc_feature    489..507
                     /label=Tet2_for
     misc_feature    508..530
                     /label=Hash_for
     misc_feature    723..745
                     /label=Hash_rev
     misc_feature    746..767
                     /label=Tet2_rev
     primer_bind     complement(820..836)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(833..855)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     rep_origin      1207..1821
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(1981..2841)
                     /function="Drug Resistance"
                     /label=Ampicillin
                     /note="Ampicillin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc atatcacgct acatcgagca tactcgacga
      541 gtgtgctcgc aaacgggacg tgcaaggtcc aacgtataag gttccctata ccacagaaca
      601 cggacgggta tgctacaccg ggatttctaa cgcccgttcg gccgcacatc tgggacttaa
      661 ttgtgctagc aacttagctg ttcatgtgcc tagtgacgat atgtcacgtt gtcccgttgg
      721 gatcgctgac actatagact gctgcgctga aaaggtggca tcaatctgca gatcagttct
      781 ggaccagcga gctgtgctgc gactcgtggc gtaatcatgg tcatagctgt ttcctgtgtg
      841 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      901 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      961 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg tcgcttaccg gatacctgtc
     1381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2821 acggaaatgt tgaatactca tactctacct ttttcaatat tattgaagca tttatcaggg
     2881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2941 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3001 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
