LOCUS       Exported                3059 bp ds-DNA     circular SYN 11-JAN-2019
DEFINITION  dog2_896196207 in pUCIDT-AMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 11, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="pUCIDT-AMP"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..789
                     /function="Reference Gene"
                     /label=dog2_896196207
                     /note="dog2_896196207"
     misc_feature    489..507
                     /label=Tet2_for
     misc_feature    508..530
                     /label=Hash_for
     misc_feature    741..763
                     /label=Hash_rev
     misc_feature    764..785
                     /label=Tet2_rev
     primer_bind     complement(838..854)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(851..873)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     rep_origin      1225..1839
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(1999..2859)
                     /function="Drug Resistance"
                     /label=Ampicillin
                     /note="Ampicillin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atgaattcgt cgtcgtcccc tcaaactatc gcgctctgac gcgtcatcga cagagcacgt
      541 cgtgtcgcgt cttccataat taaaacgcag gactcgcggg aacacccatc ggatcgcatc
      601 cgaagtatgg ataggactaa aggaaaccgc gtgtgcttgc agtggaacct ctccaccttc
      661 gccaatgtta aatgacctaa cattgacagg aatagcgcag ctcttgcaca tgccctgtcg
      721 aacgcatcaa gcgtgggcta tcgtagatat acagcagagc tgcgctgaaa aggtggcatc
      781 aatctgcaga tcagttctgg accagcgagc tgtgctgcga ctcgtggcgt aatcatggtc
      841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgtc
     1381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1681 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2821 aaagggaata agggcgacac ggaaatgttg aatactcata ctctaccttt ttcaatatta
     2881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3001 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
