LOCUS Exported 10569 bp ds-DNA circular SYN 29-AUG-2019 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 10569) AUTHORS AVA TITLE Direct Submission JOURNAL Exported Wednesday, Mar 20, 2019 from SnapGene 4.3.4 https://www.snapgene.com REFERENCE 2 (bases 1 to 10569) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Aug 29, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10569 /organism="unspecified" /mol_type="genomic DNA" promoter 8..387 /label=CMV enhancer promoter 388..591 /label=CMV promoter promoter 633..651 /label=T7 promoter CDS 665..721 /codon_start=-1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="MKRTADGSEFESPKKKRKV" CDS 722..4822 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(H840A) /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 4922..6952 /codon_start=-1 /label=MMLV RT /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP" misc_feature 5519..5521 /label=D200N misc_feature 5837..5839 /label=T306K misc_feature 5858..5860 /label=W313F misc_feature 5891 /label=sense mut misc_feature 5909..5911 /label=T330P misc_feature 6728..6730 /label=L603W CDS 6965..7015 /codon_start=-1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="KRTADGSEFEPKKKRKV" CDS 7025..7081 /codon_start=-1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /translation="ATNFSLLKQAGDVEENPGP" CDS 7082..7798 /codon_start=1 /label=GFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7811..7831 /codon_start=1 /label=SV40 NLS /translation="PKKKRKV" polyA_signal 7886..8110 /label=bGH poly(A) signal misc_feature 7987 /label=mut protein_bind 8205..8221 /label=lac operator /bound_moiety="lac repressor encoded by lacI" promoter complement(8229..8259) /label=lac promoter protein_bind 8274..8295 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" rep_origin complement(8583..9171) /direction=LEFT /label=ori CDS complement(9342..10202) /codon_start=-1 /gene="bla" /product="beta-lactamase" /label=AmpR /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10203..10307) /gene="bla" /label=AmpR promoter ORIGIN 1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg 601 aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc 661 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt 721 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat 781 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca 841 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc 901 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta 961 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact 1021 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa 1081 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa 1141 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat 1201 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt 1261 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat 1321 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg 1381 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct 1441 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga 1501 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca 1561 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct 1621 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat 1681 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca 1741 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg 1801 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga 1861 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa 1921 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc 1981 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga 2041 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag 2101 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt 2161 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa 2221 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta 2281 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag 2341 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt 2401 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc 2461 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat 2521 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct 2581 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca 2641 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag 2701 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga 2761 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag 2821 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca 2881 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt 2941 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat 3001 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat 3061 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt 3121 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga 3181 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat 3241 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga 3301 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa 3361 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac 3421 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct 3481 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac 3541 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa 3601 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta 3661 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta 3721 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat 3781 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa 3841 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc 3901 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc 3961 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca 4021 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc 4081 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta 4141 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa 4201 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt 4261 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta 4321 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca 4381 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca 4441 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca 4501 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat 4561 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc 4621 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc 4681 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga 4741 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga 4801 cctgtctcag ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac 4861 accaggaaca agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcag 4921 caccctaaat atagaagatg agtatcggct acatgagacc tcaaaagagc cagatgtttc 4981 tctagggtcc acatggctgt ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg 5041 actggcagtt cgccaagctc ctctgatcat acctctgaaa gcaacctcta cccccgtgtc 5101 cataaaacaa taccccatgt cacaagaagc cagactgggg atcaagcccc acatacagag 5161 actgttggac cagggaatac tggtaccctg ccagtccccc tggaacacgc ccctgctacc 5221 cgttaagaaa ccagggacta atgattatag gcctgtccag gatctgagag aagtcaacaa 5281 gcgggtggaa gacatccacc ccaccgtgcc caacccttac aacctcttga gcgggctccc 5341 accgtcccac cagtggtaca ctgtgcttga tttaaaggat gcctttttct gcctgagact 5401 ccaccccacc agtcagcctc tcttcgcctt tgagtggaga gatccagaga tgggaatctc 5461 aggacaattg acctggacca gactcccaca gggtttcaaa aacagtccca ccctgtttaa 5521 tgaggcactg cacagagacc tagcagactt ccggatccag cacccagact tgatcctgct 5581 acagtacgtg gatgacttac tgctggccgc cacttctgag ctagactgcc aacaaggtac 5641 tcgggccctg ttacaaaccc tagggaacct cgggtatcgg gcctcggcca agaaagccca 5701 aatttgccag aaacaggtca agtatctggg gtatcttcta aaagagggtc agagatggct 5761 gactgaggcc agaaaagaga ctgtgatggg gcagcctact ccgaagaccc ctcgacaact 5821 aagggagttc ctagggaagg caggcttctg tcgcctcttc atccctgggt ttgcagaaat 5881 ggcagccccc ctgtaccctc tcaccaaacc ggggactctg tttaattggg gcccagacca 5941 acaaaaggcc tatcaagaaa tcaagcaagc tcttctaact gccccagccc tggggttgcc 6001 agatttgact aagccctttg aactctttgt cgacgagaag cagggctacg ccaaaggtgt 6061 cctaacgcaa aaactgggac cttggcgtcg gccggtggcc tacctgtcca aaaagctaga 6121 cccagtagca gctgggtggc ccccttgcct acggatggta gcagccattg ccgtactgac 6181 aaaggatgca ggcaagctaa ccatgggaca gccactagtc attctggccc cccatgcagt 6241 agaggcacta gtcaaacaac cccccgaccg ctggctttcc aacgcccgga tgactcacta 6301 tcaggccttg cttttggaca cggaccgggt ccagttcgga ccggtggtag ccctgaaccc 6361 ggctacgctg ctcccactgc ctgaggaagg gctgcaacac aactgccttg atatcctggc 6421 cgaagcccac ggaacccgac ccgacctaac ggaccagccg ctcccagacg ccgaccacac 6481 ctggtacacg gatggaagca gtctcttaca agagggacag cgtaaggcgg gagctgcggt 6541 gaccaccgag accgaggtaa tctgggctaa agccctgcca gccgggacat ccgctcagcg 6601 ggctgaactg atagcactca cccaggccct aaagatggca gaaggtaaga agctaaatgt 6661 ttatactgat agccgttatg cttttgctac tgcccatatc catggagaaa tatacagaag 6721 gcgtgggtgg ctcacatcag aaggcaaaga gatcaaaaat aaagacgaga tcttggccct 6781 actaaaagcc ctctttctgc ccaaaagact tagcataatc cattgtccag gacatcaaaa 6841 gggacacagc gccgaggcta gaggcaaccg gatggctgac caagcggccc gaaaggcagc 6901 catcacagag actccagaca cctctaccct cctcatagaa aattcatcac cctctggcgg 6961 ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtcggaag 7021 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc 7081 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 7141 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 7201 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 7261 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 7321 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 7381 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 7441 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 7501 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 7561 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 7621 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 7681 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 7741 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc 7801 tggtggttct cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga 7861 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 7921 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 7981 aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 8041 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 8101 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc 8161 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 8221 attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg 8281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 8341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 8461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 8521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 8581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 8761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 8821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 8881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 8941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9001 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 9061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 9301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 10021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 10081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 10141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 10201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 10321 aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact 10381 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg 10441 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc 10501 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 10561 gccagatat //