LOCUS       pPU-huTRIM5⍺-miR30-L1221        8007 bp ds-DNA     circular SYN 29-AUG-2019
DEFINITION  Final.
ACCESSION   urn.local...ht-aw3pfuq
VERSION     .
KEYWORDS    pPU-HA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tue, Feb 10, 2015 from SnapGene Viewer 2.6.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     rep_origin      236..579
                     /note="SV40ori"
                     /note="SV40 ori/prom"
     promoter        580..1161
                     /note="CMVp"
                     /note="CMVieProm"
                     /modified_by="User"
                     /label="CMV promoter"
     5'UTR           1180..1276
                     /note="5'R"
                     /note="5' U3"
     5'UTR           1277..1361
                     /note="5'U5"
                     /note="5' U5"
     CDS             1515..1882
                     /codon_start=1
                     /note="gag"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*ND
                     SQLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*
                     TR*RKSKTKVRPPHSKRPAA"
     CDS             1883..2738
                     /codon_start=1
                     /note="env"
                     /note="env sequence"
                     /translation="SSDLEEEI*GTIGEVNYINIK**KLNH*E*HPPRQREEWCREKK
                     EQWE*ELCSLGSWEQQEALWAQRQ*R*RYRPDNYCLV*CSSRTIC*GLLRRNSICCNS
                     QSGASSSSRQESWLWKDT*RINSSWGFGVALENSFAPLLCLGMLVGVINLWNRFGITR
                     PGWSGTEKLTITQA*YTP*LKNRKTSKKRMNKNYWN*INGQVCGIGLT*QIGCGI*NY
                     S****EAW*V*E*FLLYFL**IELGRDIHHYRFRPTSQPRGDPTGPKE*KKKVERETE
                     TDPFD**TD"
     misc_signal     2026..2265
                     /note="RRE"
     misc_feature    2764..2941
                     /created_by="User"
                     /label="Charneau"
     misc_signal     2792..2807
                     /note="cPPT"
     misc_signal     2871..2911
                     /note="CTS"
                     /note="Charneau Central Termination Signal"
     promoter        2950..3451
                     /note="SFFVp"
                     /note="SFFVprom"
                     /modified_by="User"
                     /label="SFFV promoter"
     CDS             3464..4060
                     /codon_start=1
                     /note="puro"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
                     /modified_by="User"
                     /label="PuroR"
     CDS             4061..4288
                     /codon_start=1
                     /note="ubi K48R"
                     /note="Ubi K48R"
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
                     FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
                     /modified_by="User"
                     /label="Ub K48R"
     modified_base   4202..4204
                     /created_by="User"
                     /label="K48R"
     gene            4289..5767
                     /created_by="User"
                     /modified_by="User"
                     /Color="#4268c8"
                     /modified_by="User"
                     /label="Human TRIM5alpha"
     modified_base   4391..4412
                     /created_by="User"
                     /modified_by="User"
                     /label="shRNA resistance"
     modified_base   4393
                     /created_by="User"
                     /label
     modified_base   4396
                     /created_by="User"
                     /label
     modified_base   4399
                     /created_by="User"
                     /label
     modified_base   4402
                     /created_by="User"
                     /label
     modified_base   4403
                     /created_by="User"
                     /label
     modified_base   4405
                     /created_by="User"
                     /label
     modified_base   4408
                     /created_by="User"
                     /label
     modified_base   4411
                     /created_by="User"
                     /label
     misc_feature    5779..5931
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="miR30"
     misc_signal     5957..5972
                     /note="PPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PPT"
     3'UTR           5972..5996
                     /created_by="User"
                     /label="3' deltaU3"
     3'UTR           6005..6101
                     /note="3'R"
                     /note="3' R"
     3'UTR           6102..6185
                     /note="3'U5"
                     /note="3' U5"
     oriT            6196..6866
                     /note="colE1"
     marker          complement(7014..7874)
                     /created_by="User"
                     /label="Ampicillin marker"
     promoter        complement(7916..7944)
                     /created_by="User"
                     /label="Amp promoter"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctt tgacattgat tattgactag
      601 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      661 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      721 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      781 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      841 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      901 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      961 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1021 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1081 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1141 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     1201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1501 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1561 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1621 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1681 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1741 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1801 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1861 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1921 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1981 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2041 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2101 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2161 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2221 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2281 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2341 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2401 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2461 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2521 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2581 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2641 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2701 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2761 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2821 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2881 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2941 gttaattaac tgcagccccg ataaaataaa agattttatt tagtctccag aaaaaggggg
     3001 gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     3061 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     3121 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     3181 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     3241 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     3301 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     3361 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     3421 cagtcctccg acagactgag tcgcccgggg gtctagtgcc accatgaccg agtacaagcc
     3481 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     3541 gttcgccgac taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt
     3601 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     3661 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     3721 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     3781 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3841 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3901 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     3961 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4021 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc atgcagatct ttgtgaagac
     4081 cctcactggc aaaaccatca cccttgaggt cgagcccagt gacaccattg agaatgtcaa
     4141 agccaaaatt caagacaagg agggtatccc acctgaccag cagcgtctga tatttgccgg
     4201 cagacagctg gaggatggcc gcactctctc agactacaac atccagaaag agtccaccct
     4261 gcacctggtg ttgcgcctgc gaggtggcat ggcttctggg atcctggtta atgtaaagga
     4321 ggaggtgacc tgccccatct gcctggaact cctgacacaa cccctgagcc tggactgcgg
     4381 ccacagcttc tgtcaggcct gtttaacagc taaccacaag aagtccatgc tagacaaagg
     4441 agagagtagc tgccctgtgt gccggatcag ttaccagcct gagaacatac ggcctaatcg
     4501 gcatgtagcc aacatagtgg agaagctcag ggaggtcaag ttgagcccag aggggcagaa
     4561 agttgatcat tgtgcacgcc atggagagaa acttctactc ttctgtcagg aggacgggaa
     4621 ggtcatttgc tggctttgtg agcggtctca ggagcaccgt ggtcaccaca cgttcctcac
     4681 agaggaggtt gcccgggagt accaagtgaa gctccaggca gctctggaga tgctgaggca
     4741 gaagcagcag gaagctgaag agttggaagc tgacatcaga gaagagaaag cttcctggaa
     4801 gactcaaata cagtatgaca aaaccaacgt cttggcagat tttgagcaac tgagagacat
     4861 cctggactgg gaggagagca atgagctgca aaacctggag aaggaggagg aagacattct
     4921 gaaaagcctt acgaactctg aaactgagat ggtgcagcag acccagtccc tgagagagct
     4981 catctcagat ctggagcatc ggctgcaggg gtcagtgatg gagctgcttc agggtgtgga
     5041 tggcgtcata aaaaggacgg agaacgtgac cttgaagaag ccagaaactt ttccaaaaaa
     5101 tcaaaggaga gtgtttcgag ctcctgatct gaaaggaatg ctagaagtgt ttagagagct
     5161 gacagatgtc cgacgctact gggttgatgt gacagtggct ccaaacaaca tttcatgtgc
     5221 tgtcatttct gaagataaga gacaagtgag ctctccgaaa ccacagataa tatatggggc
     5281 acgagggaca agataccaga catttgtgaa tttcaattat tgtactggca tcctgggctc
     5341 tcaaagtatc acatcaggga aacattactg ggaggtagac gtgtccaaga aaactgcttg
     5401 gatactgggg gtatgtgctg gcttccaacc tgatgcaatg tgtaatattg aaaaaaatga
     5461 aaattatcaa cctaaatacg gctactgggt tatagggtta gaggaaggag ttaaatgtag
     5521 tgctttccag gatagttcct tccatactcc ttctgttcct ttcattgtgc ccctctctgt
     5581 gattatttgt cctgatcgtg ttggagtttt cctagactat gaggcttgca ctgtctcatt
     5641 cttcaatatc acaaaccatg gatttctcat ctataagttt tctcactgtt ctttttctca
     5701 gcctgtattt ccatatttaa atcctagaaa atgtggagtc cccatgactc tgtgctcacc
     5761 aagctcttga gcggccgcct tcttaaccca acagaaggct cgagaaggta tattgctgtt
     5821 gacagtgagc gcacaaacgc tctcatcgac aagtagtgaa gccacagatg tacttgtcga
     5881 tgagagcgtt tgtatgccta ctgcctcgga cttcaagggg ctagaattcg gcagctgtag
     5941 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaactgca tccggactgt
     6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6181 agcagggccc gtttcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7981 acatttcccc gaaaagtgcc acctgac
//
