LOCUS       Exported               11504 bp ds-DNA     circular SYN 08-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Boeke Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          682..700
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             279..321
                     /label=LacZ alpha ORF
     misc_feature    325..681
                     /label=rrk1 gene -1 to -358
     promoter        325..681
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_feature    682..700
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    701..708
                     /label=Not1 Cutting Site
     misc_RNA        709..760
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             792..808
                     /label=LacZ alpha ORF(1)
     promoter        complement(871..889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1060..1515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(1951..2745)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      3365..4148
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     4300..4357
                     /label=YZ1036
     misc_feature    4330..4333
                     /label=CAGT
     promoter        4334..5075
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             5096..9133
                     /codon_start=1
                     /product="FnCpf1-NLS-3xHA tag"
                     /label=huFnCpf1
                     /note="derived from Francisella novicida U112 (FnCpf1); 
                     optimized for expression in human"
                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK
                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT
                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID
                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK
                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF
                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID
                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL
                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL
                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA
                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV
                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK
                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH
                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF
                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF
                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK
                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD
                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV
                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT
                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE
                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR
                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL
                     VIKNEEYFEFVQNRNNKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPD
                     YA"
     primer_bind     6840..6864
                     /label=YZ1516
     misc_feature    7844..7846
                     /label=D917A mutation for dFnCpf1
     CDS             8996..9043
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9050..9130
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      9146..9393
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(9375..9431)
                     /label=YZ1037
     misc_feature    9401..9404
                     /label=TTTT
     promoter        9599..9703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9704..10564
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10735..11323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcgg atccactagt aacggccgcc
      301 agtgtgctgg aattcgccct tccttttgct tatgttggtg gtagttggca tgcgtagact
      361 gatgactagt cagcaaggag cgtagaacag tcacactcgt tatatatgtg cttccaagaa
      421 aactcaagaa tttaccatta gcaaacactt ttttgaaatg ttagacattt aaatgacgaa
      481 ggcatataga agctttgaat aggtgttgta aagtgttgat ttatgtgacg ctgagggtgc
      541 gcatgaaagg aatgttgggt cacgattatt aaacagtttg ctagcttgga cacttgagta
      601 ttggaagttg ttgaattcta aaaaactttc agttgatttg aatagttgct gttgccaaaa
      661 aacataacct gtaccgaaga aaatttctac tgttgtagat gcggccgccc tgtcaccgga
      721 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta
      781 gagtcgacct gaagggcgaa ttctgcagga attcgatatc aagcttatcg ataccgtcga
      841 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1021 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     1081 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1141 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1201 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1261 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1321 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1381 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1441 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1501 taacgtttac aatttagctt gtgatattga cgaaactttt tgacatctaa tttattctgt
     1561 tccaacacca atgtttataa ccaagtttta tcttgtttgt ctacatggta ttttacattc
     1621 atctacatac atctttcatt ggctttgtac atagttatca ttacaagtct aaaaaaattc
     1681 actcttttct tattcaatgt caatccaaga gaaaagattg tggtaatgtt gtaggagcat
     1741 gtttaataaa ttactatagc aaattacttt ttattcccaa ggtgtttatc tataatagtt
     1801 aatattttag tcgctacata aaattttacc aaagagtact tgtatactaa ttctaaatgc
     1861 cttctgacat aaaacgccta ggaaaacaaa cgcaaacaag gcatcgactt tttcaataac
     1921 caaccaaaaa aattttacat tagtcttttt ttaatgctga gaaagtcttt gctgatatgc
     1981 cttccaacca gcttctctat atctcttggc ttcgacaaca ggattacgac cagctccata
     2041 gactccacga ccaacaatga tgatatcgct accgcagttt acaatcactt cttcaggagt
     2101 acgatattgc tgtcccagcc cgtctccttt aacatccaag ccgataccag gggacatagt
     2161 tatgtagtcg ctttgaaggt taggaaatcg acgaccagct ataaagccaa agcaaaaatc
     2221 ggtatgcttc tcaaaccatt ctaaggtttt ctctgtgtag gaaccagtag ccaaagagcc
     2281 tttggaagac atttcagcca aaagcaagag tccacgtccc aaaggtaaac caacttcttt
     2341 gaggccttgt ataataccct cgcctggcac tgtatggcaa tttgtgatat gagcccaaga
     2401 agcaattttg tacacaccag atgcatattg tagcttgacg gtatttccaa tgtctgcgaa
     2461 tttgcgatcc tcaaagataa gaaaacgatg ctttttacct aaggccacca gtttttctac
     2521 catatcctgg tcgaaatcct cgacaacgtc aatatgtgtc ttgataacac agacataggg
     2581 tccaatttta tctaccaatt ctaagatttc ggatttcttc gtcaaatcga ccgcgactga
     2641 caagttgctt tgcttttctt ccatcaaagc caacaattcc ttggcaatgg gatttttcat
     2701 cccctcagct ctagctgaat agctttgaaa tactctagca tccataactt tgcttttaaa
     2761 cctttaattt cgatccaagc aaaaaagagg ttcttggtag gacaatacgg taagaaaaca
     2821 cgacatgtgc agagatgccg acgaagcata gttaaactgg gatggtaaaa tcaattaaga
     2881 atttataaag acaaaattgt ataagtctct aaaacatctt aattatacct cacagaacta
     2941 tctaaaatat attcacaaag tgcaaacatt atcatgaaaa agaaccattt taatttaaag
     3001 caagggcatt aaggcttatt tacagaattt cttacttttg taaagattat aaggctgatt
     3061 atctttttca ccatgccaaa aattacacaa gatagaatgg atgtttgaaa ttaaacgtga
     3121 gtatacaaac aaatacacta ggtaaatcga aacatttttt tctccattaa gtaacaaatt
     3181 cctatttaga gaaagaatgc tgagtagatt aaataatcta tacaaacttt tttaacacaa
     3241 atgcatacat atagccagtg ggatttgtag ctagtcgatc gactctagag gaaattcgag
     3301 tctaactcct taaccactcg gacatagtga cttatctgac actcattgta aattaatata
     3361 tatataggca ttttgtttag ttaaaggtac ttaagtaatt agtataaacg aaccaatttt
     3421 ataatcagga agttaagtga atggtagcac atgtcgtaaa aattgtgaat ttttattgaa
     3481 taatatttta aatacaagcc tttctagact aggtatactc ataaacatat atgagcaaaa
     3541 ggatagagga gattacattg catcttctac aaatttattt attgcccttt actgaaaaat
     3601 taaataatga gtactaaatg ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg
     3661 cattcttcat gaattaaagt tgcatataag gcatattgaa agtaatagta ctaaaacagc
     3721 agttagcgaa aattaataga attatattcg caagacaatt gtgacattaa attaaaaaat
     3781 tgtagaattt ttactatcct ctttaacgcc atgagccttt ataaaaaggt taaattagtt
     3841 ttaacattct ttttttgagt aagagtttac aatttatcaa aacctgtgtt attatattca
     3901 ttagtttcaa tttattagca tctagagaaa aatcaattgg cagttacatt gttggaattt
     3961 atgaagaaaa agaatctaca acggagaata agttgctgat cgctttccct aaaattgtat
     4021 attttgctga gcttattttg acatttcgtt gaagttttct ctaattcgca ttcattttaa
     4081 gtaaaacaat gagaaataaa attacaaaaa atacaaatta aaatacaatt tttagctata
     4141 attatagacg atgcccttgt atcccattct gtctcgcttg cccctacttt ttatctttta
     4201 tataccataa tgaacgctgc cgctactaac cataccccga ttttacattt cggactccca
     4261 aggacgtaca aaatagaaaa ctatagaaaa aaataatcag aaaatagcat gtcatctctt
     4321 tgtaaaacgc agtgccctac aacaactaag aaaatggcta tcatgcggaa gctggtgaga
     4381 agaacagcat cgggacaagg gaaggaagaa caaagacaaa gaaaacaaaa gaaagcaatt
     4441 gaaaacaaaa caaaacaatt ttcattcctt ctcttatcat tccttttctt ttcttttctc
     4501 tcattcaacg cactccatcg tatccgtatt cctcttattt tttctctttc tctatatcca
     4561 tttctttctc tctaggtgtg tcctctctct ctcttcaatt tctctactcc gcattccaac
     4621 gcatccttcc cccaacctcc catttcctcc ttacggcccg atagcgatcg tctttccctc
     4681 gctatcactc gctaccggcc cctcctctgc accgtaacct cctacgtatt taccatatca
     4741 taaagttttt tccgacgctt atcgctgacc ccctgtcgcc ctcctattgg cttccggatt
     4801 atcttcttgt ccataaggtg atccatgctt cctgaagatt cccgaaatgt gtccactttg
     4861 gcggggaatc attccatcca cttctttctc tctcgctttc ctcattcggc gctccccttc
     4921 cgcgtctcat tggtcttccg ctccgttttt gctttgccga tgttacttgg ggagaggtgc
     4981 gataatcctt tcgcaaaaac tcggtttgac gcctcccatg gtataaatag tgggtggtgg
     5041 acaggtgcct tcgcttttct ttaagcaaga gaattgctgc aggtcgactc tagagatgag
     5101 catctaccag gagttcgtca acaagtattc actgagtaag acactgcggt tcgagctgat
     5161 cccacagggc aagacactgg agaacatcaa ggcccgaggc ctgattctgg acgatgagaa
     5221 gcgggcaaaa gactataaga aagccaagca gatcattgat aaataccacc agttctttat
     5281 cgaggaaatt ctgagctccg tgtgcatcag tgaggatctg ctgcagaatt actcagacgt
     5341 gtacttcaag ctgaagaaga gcgacgatga caacctgcag aaggacttca agtccgccaa
     5401 ggacaccatc aagaaacaga ttagcgagta catcaaggac tccgaaaagt ttaaaaatct
     5461 gttcaaccag aatctgatcg atgctaagaa aggccaggag tccgacctga tcctgtggct
     5521 gaaacagtct aaggacaatg ggattgaact gttcaaggct aactccgata tcactgatat
     5581 tgacgaggca ctggaaatca tcaagagctt caagggatgg accacatact ttaaaggctt
     5641 ccacgagaac cgcaagaacg tgtactccag caacgacatt cctacctcca tcatctaccg
     5701 aatcgtcgat gacaatctgc caaagttcct ggagaacaag gccaaatatg aatctctgaa
     5761 ggacaaagct cccgaggcaa ttaattacga acagatcaag aaagatctgg ctgaggaact
     5821 gacattcgat atcgactata agactagcga ggtgaaccag agggtctttt ccctggacga
     5881 ggtgtttgaa atcgccaatt tcaacaatta cctgaaccag tccggcatta ctaaattcaa
     5941 taccatcatt ggcgggaagt ttgtgaacgg ggagaatacc aagcgcaagg gaattaacga
     6001 atacatcaat ctgtatagcc agcagatcaa cgacaaaact ctgaagaaat acaagatgtc
     6061 tgtgctgttc aaacagatcc tgagtgatac cgagtccaag tcttttgtca ttgataaact
     6121 ggaagatgac tcagacgtgg tcactaccat gcagagcttt tatgagcaga tcgccgcttt
     6181 caagacagtg gaggaaaaat ctattaagga aactctgagt ctgctgttcg atgacctgaa
     6241 agcccagaag ctggacctga gtaagatcta cttcaaaaac gataagagtc tgacagacct
     6301 gtcacagcag gtgtttgatg actattccgt gattgggacc gccgtcctgg agtacattac
     6361 acagcagatc gctccaaaga acctggataa tccctctaag aaagagcagg aactgatcgc
     6421 taagaaaacc gagaaggcaa aatatctgag tctggaaaca attaagctgg cactggagga
     6481 gttcaacaag cacagggata ttgacaaaca gtgccgcttt gaggaaatcc tggccaactt
     6541 cgcagccatc cccatgattt ttgatgagat cgcccagaac aaagacaatc tggctcagat
     6601 cagtattaag taccagaacc agggcaagaa agacctgctg caggcttcag cagaagatga
     6661 cgtgaaagcc atcaaggatc tgctggacca gaccaacaat ctgctgcaca agctgaaaat
     6721 cttccatatt agtcagtcag aggataaggc taatatcctg gataaagacg aacacttcta
     6781 cctggtgttc gaggaatgtt acttcgagct ggcaaacatt gtccccctgt ataacaagat
     6841 taggaactac atcacacaga agccttactc tgacgagaag tttaaactga acttcgaaaa
     6901 tagtaccctg gccaacgggt gggataagaa caaggagcct gacaacacag ctatcctgtt
     6961 catcaaggat gacaagtact atctgggagt gatgaataag aaaaacaata agatcttcga
     7021 tgacaaagcc attaaggaga acaaagggga aggatacaag aaaatcgtgt ataagctgct
     7081 gcccggcgca aataagatgc tgcctaaggt gttcttcagc gccaagagta tcaaattcta
     7141 caacccatcc gaggacatcc tgcggattag aaatcactca acacatacta agaacgggag
     7201 cccccagaag ggatatgaga aatttgagtt caacatcgag gattgcagga agtttattga
     7261 cttctacaag cagagcatct ccaaacaccc tgaatggaag gattttggct tccggttttc
     7321 cgacacacag agatataact ctatcgacga gttctaccgc gaggtggaaa atcaggggta
     7381 taagctgact tttgagaaca tttctgaaag ttacatcgac agcgtggtca atcagggaaa
     7441 gctgtacctg ttccagatct ataacaaaga tttttcagca tacagcaagg gcagaccaaa
     7501 cctgcataca ctgtactgga aggccctgtt cgatgagagg aatctgcagg acgtggtcta
     7561 taaactgaac ggagaggccg aactgtttta ccggaagcag tctattccta agaaaatcac
     7621 tcacccagct aaggaggcca tcgctaacaa gaacaaggac aatcctaaga aagagagcgt
     7681 gttcgaatac gatctgatta aggacaagcg gttcaccgaa gataagttct ttttccattg
     7741 tccaatcacc attaacttca agtcaagcgg cgctaacaag ttcaacgacg agatcaatct
     7801 gctgctgaag gaaaaagcaa acgatgtgca catcctgagc attgaccgag gagagcggca
     7861 tctggcctac tataccctgg tggatggcaa agggaatatc attaagcagg atacattcaa
     7921 catcattggc aatgaccgga tgaaaaccaa ctaccacgat aaactggctg caatcgagaa
     7981 ggatagagac tcagctagga aggactggaa gaaaatcaac aacattaagg agatgaagga
     8041 aggctatctg agccaggtgg tccatgagat tgcaaagctg gtcatcgaat acaatgccat
     8101 tgtggtgttc gaggatctga acttcggctt taagaggggg cgctttaagg tggaaaaaca
     8161 ggtctatcag aagctggaga aaatgctgat cgaaaagctg aattacctgg tgtttaaaga
     8221 taacgagttc gacaagaccg gaggcgtcct gagagcctac cagctgacag ctccctttga
     8281 aactttcaag aaaatgggaa aacagacagg catcatctac tatgtgccag ccggattcac
     8341 ttccaagatc tgccccgtga ccggctttgt caaccagctg taccctaaat atgagtcagt
     8401 gagcaagtcc caggaatttt tcagcaagtt cgataagatc tgttataatc tggacaaggg
     8461 gtacttcgag ttttccttcg attacaagaa cttcggcgac aaggccgcta aggggaaatg
     8521 gaccattgcc tccttcggat ctcgcctgat caactttcga aattccgata aaaaccacaa
     8581 ttgggacact agggaggtgt acccaaccaa ggagctggaa aagctgctga aagactactc
     8641 tatcgagtat ggacatggcg aatgcatcaa ggcagccatc tgtggcgaga gtgataagaa
     8701 atttttcgcc aagctgacct cagtgctgaa tacaatcctg cagatgcgga actcaaagac
     8761 cgggacagaa ctggactatc tgattagccc cgtggctgat gtcaacggaa acttcttcga
     8821 cagcagacag gcacccaaaa atatgcctca ggatgcagac gccaacgggg cctaccacat
     8881 cgggctgaag ggactgatgc tgctgggccg gatcaagaac aatcaggagg ggaagaagct
     8941 gaacctggtc attaagaacg aggaatactt cgagtttgtc cagaatagaa ataacaaaag
     9001 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct acccatacga
     9061 tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat atgatgtccc
     9121 cgactatgcc taatctcttc tcgagtcatg taattagtta tgtcacgctt acattcacgc
     9181 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     9241 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     9301 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     9361 aaggttttgg gacgctcgaa ggctttaatt tgcgatcccc ttttcgtttg caagaagaaa
     9421 ggaaacaatg gagaagagat catccatttg tatgtaaatt ttagtaaact tgaagaaatc
     9481 actaacaact tctcttactt agggattcta tgcaaaacct tgtaaatcat ctgatggagg
     9541 actcgattta atgaattgga aggttggatt tccaggtggc acttttcggg gaaatgtgcg
     9601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10381 ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta tcattgcagc
    10441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10861 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    10921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11101 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
    11161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
