LOCUS       Exported               12436 bp ds-DNA     circular SYN 26-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Boeke Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1487..1505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1545..1563
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..259
                     /label=T7
     misc_feature    314..1483
                     /label=fba1 promoter
     misc_feature    1487..1505
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    complement(1507..1512)
                     /label=BsaI
                     /note="BsaI recognition site GGTCTC"
     misc_feature    1538..1543
                     /label=BsaI
                     /note="BsaI recognition site GGTCTC"
     misc_feature    1545..1563
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    1568..1807
                     /gene="TEFt"
                     /product="TEF1 terminator of A. gossypii"
                     /label=TEF1 terminator of A. gossypii
     primer_bind     complement(1831..1847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1992..2447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2883..3677)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4297..5080
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     5232..5289
                     /label=YZ1036
     promoter        5266..6007
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             6028..10065
                     /codon_start=1
                     /product="FnCpf1-NLS-3xHA tag"
                     /label=huFnCpf1
                     /note="derived from Francisella novicida U112 (FnCpf1); 
                     optimized for expression in human"
                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK
                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT
                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID
                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK
                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF
                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID
                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL
                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL
                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA
                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV
                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK
                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH
                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF
                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF
                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK
                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD
                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV
                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT
                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE
                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR
                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL
                     VIKNEEYFEFVQNRNNKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPD
                     YA"
     CDS             9928..9975
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9982..10062
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      10078..10325
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10307..10363)
                     /label=YZ1037
     promoter        10531..10635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10636..11496
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11667..12255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 aatacgactc actataggga aagcttgcat gcctgcaggt cgactctaga ggatcggccg
      301 gcccgcggcc gcacagtctc atactcttct agtcaagtct catcctcttc cactctctca
      361 attccttttt atcaatgttt cttcctcttg ttatgtacac aaacgatgat ctcttctctc
      421 acctcattgc tggactaata ttactcgtgg ccaagcgaaa cacaagtggt actagataac
      481 caaaaaatga taaagcattc aatcaatcaa accacttacg aaattcatac tcccgtaaac
      541 gaatgccggt cggacgcatg gaaaatcatc aatacgtgag agagctactt agttgtgcac
      601 tttgtatatg tccaaaaata cttttcattt cacatacctg cttgtgtaaa gacagtgatg
      661 aaatgcggtg caaagtgtct attcttacat tgatggtaaa gctaattcaa gcatgcttgg
      721 aagaagaatt gccttgcatt tcttgatcat ttgacccagg cgacaactat aaaataagaa
      781 tactaccgaa gatgcagatg gggtgtgtgg gcacaacacc atcgaaccga accgagacgg
      841 ccagactgtg ataacaaaag aaatgcatta gtttagttgg ctggttcaag ttggttgagt
      901 agaaaaatcc atcactcaaa ctgggaagta gggatggctg ggagaatgga gaagcgagac
      961 gagacgagac aagacaagag gagatgaaat gaaattaatg aaaagaaatg aatagaaatg
     1021 aaatagacat atgaatagta tagggcaggt tagatgtttg tatttataaa tgtttaatgc
     1081 ttaggtttgt atttatgttt acgttggtaa tgtttctttt gtttgtgttt gtgcatcggg
     1141 tgaacgagtt ggttaccgcc gccgccgctt ccttttgcct tgacacacac cacgcaacgt
     1201 gttacggttc ttgaacggaa ttgcgtactt cgatgcagag cgctatcggg gggattgggg
     1261 atggtaaatg gaagaaatca aaaacttctt ttgacgtcaa cccctaaaac aggggacggt
     1321 gattggtggt aaccacggac cggataggta tcgatatgca catcaggtga gaagtttttc
     1381 cgaggccaac tccggctcca tggtatataa tagagggttg cgcgctactg ttgcagtatc
     1441 atacttccaa aggtcgtata gaacacacac acaaattagc aaaatgaatt tctactgttg
     1501 tagatcgaga cctccctgca agaccatcta caaagttggt ctccaatttc tactgttgta
     1561 gattgagtca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1621 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1681 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1741 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1801 gtgtcgaagc ggccgcgcga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     1861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1981 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2401 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttagc ttgtgatatt
     2461 gacgaaactt tttgacatct aatttattct gttccaacac caatgtttat aaccaagttt
     2521 tatcttgttt gtctacatgg tattttacat tcatctacat acatctttca ttggctttgt
     2581 acatagttat cattacaagt ctaaaaaaat tcactctttt cttattcaat gtcaatccaa
     2641 gagaaaagat tgtggtaatg ttgtaggagc atgtttaata aattactata gcaaattact
     2701 ttttattccc aaggtgttta tctataatag ttaatatttt agtcgctaca taaaatttta
     2761 ccaaagagta cttgtatact aattctaaat gccttctgac ataaaacgcc taggaaaaca
     2821 aacgcaaaca aggcatcgac tttttcaata accaaccaaa aaaattttac attagtcttt
     2881 ttttaatgct gagaaagtct ttgctgatat gccttccaac cagcttctct atatctcttg
     2941 gcttcgacaa caggattacg accagctcca tagactccac gaccaacaat gatgatatcg
     3001 ctaccgcagt ttacaatcac ttcttcagga gtacgatatt gctgtcccag cccgtctcct
     3061 ttaacatcca agccgatacc aggggacata gttatgtagt cgctttgaag gttaggaaat
     3121 cgacgaccag ctataaagcc aaagcaaaaa tcggtatgct tctcaaacca ttctaaggtt
     3181 ttctctgtgt aggaaccagt agccaaagag cctttggaag acatttcagc caaaagcaag
     3241 agtccacgtc ccaaaggtaa accaacttct ttgaggcctt gtataatacc ctcgcctggc
     3301 actgtatggc aatttgtgat atgagcccaa gaagcaattt tgtacacacc agatgcatat
     3361 tgtagcttga cggtatttcc aatgtctgcg aatttgcgat cctcaaagat aagaaaacga
     3421 tgctttttac ctaaggccac cagtttttct accatatcct ggtcgaaatc ctcgacaacg
     3481 tcaatatgtg tcttgataac acagacatag ggtccaattt tatctaccaa ttctaagatt
     3541 tcggatttct tcgtcaaatc gaccgcgact gacaagttgc tttgcttttc ttccatcaaa
     3601 gccaacaatt ccttggcaat gggatttttc atcccctcag ctctagctga atagctttga
     3661 aatactctag catccataac tttgctttta aacctttaat ttcgatccaa gcaaaaaaga
     3721 ggttcttggt aggacaatac ggtaagaaaa cacgacatgt gcagagatgc cgacgaagca
     3781 tagttaaact gggatggtaa aatcaattaa gaatttataa agacaaaatt gtataagtct
     3841 ctaaaacatc ttaattatac ctcacagaac tatctaaaat atattcacaa agtgcaaaca
     3901 ttatcatgaa aaagaaccat tttaatttaa agcaagggca ttaaggctta tttacagaat
     3961 ttcttacttt tgtaaagatt ataaggctga ttatcttttt caccatgcca aaaattacac
     4021 aagatagaat ggatgtttga aattaaacgt gagtatacaa acaaatacac taggtaaatc
     4081 gaaacatttt tttctccatt aagtaacaaa ttcctattta gagaaagaat gctgagtaga
     4141 ttaaataatc tatacaaact tttttaacac aaatgcatac atatagccag tgggatttgt
     4201 agctagtcga tcgactctag aggaaattcg agtctaactc cttaaccact cggacatagt
     4261 gacttatctg acactcattg taaattaata tatatatagg cattttgttt agttaaaggt
     4321 acttaagtaa ttagtataaa cgaaccaatt ttataatcag gaagttaagt gaatggtagc
     4381 acatgtcgta aaaattgtga atttttattg aataatattt taaatacaag cctttctaga
     4441 ctaggtatac tcataaacat atatgagcaa aaggatagag gagattacat tgcatcttct
     4501 acaaatttat ttattgccct ttactgaaaa attaaataat gagtactaaa tgataaaaag
     4561 cgctcagtac aagaaagatt gcaaaaatat tgcattcttc atgaattaaa gttgcatata
     4621 aggcatattg aaagtaatag tactaaaaca gcagttagcg aaaattaata gaattatatt
     4681 cgcaagacaa ttgtgacatt aaattaaaaa attgtagaat ttttactatc ctctttaacg
     4741 ccatgagcct ttataaaaag gttaaattag ttttaacatt ctttttttga gtaagagttt
     4801 acaatttatc aaaacctgtg ttattatatt cattagtttc aatttattag catctagaga
     4861 aaaatcaatt ggcagttaca ttgttggaat ttatgaagaa aaagaatcta caacggagaa
     4921 taagttgctg atcgctttcc ctaaaattgt atattttgct gagcttattt tgacatttcg
     4981 ttgaagtttt ctctaattcg cattcatttt aagtaaaaca atgagaaata aaattacaaa
     5041 aaatacaaat taaaatacaa tttttagcta taattataga cgatgccctt gtatcccatt
     5101 ctgtctcgct tgcccctact ttttatcttt tatataccat aatgaacgct gccgctacta
     5161 accatacccc gattttacat ttcggactcc caaggacgta caaaatagaa aactatagaa
     5221 aaaaataatc agaaaatagc atgtcatctc tttgtaaaac gcagtgccct acaacaacta
     5281 agaaaatggc tatcatgcgg aagctggtga gaagaacagc atcgggacaa gggaaggaag
     5341 aacaaagaca aagaaaacaa aagaaagcaa ttgaaaacaa aacaaaacaa ttttcattcc
     5401 ttctcttatc attccttttc ttttcttttc tctcattcaa cgcactccat cgtatccgta
     5461 ttcctcttat tttttctctt tctctatatc catttctttc tctctaggtg tgtcctctct
     5521 ctctcttcaa tttctctact ccgcattcca acgcatcctt cccccaacct cccatttcct
     5581 ccttacggcc cgatagcgat cgtctttccc tcgctatcac tcgctaccgg cccctcctct
     5641 gcaccgtaac ctcctacgta tttaccatat cataaagttt tttccgacgc ttatcgctga
     5701 ccccctgtcg ccctcctatt ggcttccgga ttatcttctt gtccataagg tgatccatgc
     5761 ttcctgaaga ttcccgaaat gtgtccactt tggcggggaa tcattccatc cacttctttc
     5821 tctctcgctt tcctcattcg gcgctcccct tccgcgtctc attggtcttc cgctccgttt
     5881 ttgctttgcc gatgttactt ggggagaggt gcgataatcc tttcgcaaaa actcggtttg
     5941 acgcctccca tggtataaat agtgggtggt ggacaggtgc cttcgctttt ctttaagcaa
     6001 gagaattgct gcaggtcgac tctagagatg agcatctacc aggagttcgt caacaagtat
     6061 tcactgagta agacactgcg gttcgagctg atcccacagg gcaagacact ggagaacatc
     6121 aaggcccgag gcctgattct ggacgatgag aagcgggcaa aagactataa gaaagccaag
     6181 cagatcattg ataaatacca ccagttcttt atcgaggaaa ttctgagctc cgtgtgcatc
     6241 agtgaggatc tgctgcagaa ttactcagac gtgtacttca agctgaagaa gagcgacgat
     6301 gacaacctgc agaaggactt caagtccgcc aaggacacca tcaagaaaca gattagcgag
     6361 tacatcaagg actccgaaaa gtttaaaaat ctgttcaacc agaatctgat cgatgctaag
     6421 aaaggccagg agtccgacct gatcctgtgg ctgaaacagt ctaaggacaa tgggattgaa
     6481 ctgttcaagg ctaactccga tatcactgat attgacgagg cactggaaat catcaagagc
     6541 ttcaagggat ggaccacata ctttaaaggc ttccacgaga accgcaagaa cgtgtactcc
     6601 agcaacgaca ttcctacctc catcatctac cgaatcgtcg atgacaatct gccaaagttc
     6661 ctggagaaca aggccaaata tgaatctctg aaggacaaag ctcccgaggc aattaattac
     6721 gaacagatca agaaagatct ggctgaggaa ctgacattcg atatcgacta taagactagc
     6781 gaggtgaacc agagggtctt ttccctggac gaggtgtttg aaatcgccaa tttcaacaat
     6841 tacctgaacc agtccggcat tactaaattc aataccatca ttggcgggaa gtttgtgaac
     6901 ggggagaata ccaagcgcaa gggaattaac gaatacatca atctgtatag ccagcagatc
     6961 aacgacaaaa ctctgaagaa atacaagatg tctgtgctgt tcaaacagat cctgagtgat
     7021 accgagtcca agtcttttgt cattgataaa ctggaagatg actcagacgt ggtcactacc
     7081 atgcagagct tttatgagca gatcgccgct ttcaagacag tggaggaaaa atctattaag
     7141 gaaactctga gtctgctgtt cgatgacctg aaagcccaga agctggacct gagtaagatc
     7201 tacttcaaaa acgataagag tctgacagac ctgtcacagc aggtgtttga tgactattcc
     7261 gtgattggga ccgccgtcct ggagtacatt acacagcaga tcgctccaaa gaacctggat
     7321 aatccctcta agaaagagca ggaactgatc gctaagaaaa ccgagaaggc aaaatatctg
     7381 agtctggaaa caattaagct ggcactggag gagttcaaca agcacaggga tattgacaaa
     7441 cagtgccgct ttgaggaaat cctggccaac ttcgcagcca tccccatgat ttttgatgag
     7501 atcgcccaga acaaagacaa tctggctcag atcagtatta agtaccagaa ccagggcaag
     7561 aaagacctgc tgcaggcttc agcagaagat gacgtgaaag ccatcaagga tctgctggac
     7621 cagaccaaca atctgctgca caagctgaaa atcttccata ttagtcagtc agaggataag
     7681 gctaatatcc tggataaaga cgaacacttc tacctggtgt tcgaggaatg ttacttcgag
     7741 ctggcaaaca ttgtccccct gtataacaag attaggaact acatcacaca gaagccttac
     7801 tctgacgaga agtttaaact gaacttcgaa aatagtaccc tggccaacgg gtgggataag
     7861 aacaaggagc ctgacaacac agctatcctg ttcatcaagg atgacaagta ctatctggga
     7921 gtgatgaata agaaaaacaa taagatcttc gatgacaaag ccattaagga gaacaaaggg
     7981 gaaggataca agaaaatcgt gtataagctg ctgcccggcg caaataagat gctgcctaag
     8041 gtgttcttca gcgccaagag tatcaaattc tacaacccat ccgaggacat cctgcggatt
     8101 agaaatcact caacacatac taagaacggg agcccccaga agggatatga gaaatttgag
     8161 ttcaacatcg aggattgcag gaagtttatt gacttctaca agcagagcat ctccaaacac
     8221 cctgaatgga aggattttgg cttccggttt tccgacacac agagatataa ctctatcgac
     8281 gagttctacc gcgaggtgga aaatcagggg tataagctga cttttgagaa catttctgaa
     8341 agttacatcg acagcgtggt caatcaggga aagctgtacc tgttccagat ctataacaaa
     8401 gatttttcag catacagcaa gggcagacca aacctgcata cactgtactg gaaggccctg
     8461 ttcgatgaga ggaatctgca ggacgtggtc tataaactga acggagaggc cgaactgttt
     8521 taccggaagc agtctattcc taagaaaatc actcacccag ctaaggaggc catcgctaac
     8581 aagaacaagg acaatcctaa gaaagagagc gtgttcgaat acgatctgat taaggacaag
     8641 cggttcaccg aagataagtt ctttttccat tgtccaatca ccattaactt caagtcaagc
     8701 ggcgctaaca agttcaacga cgagatcaat ctgctgctga aggaaaaagc aaacgatgtg
     8761 cacatcctga gcattgaccg aggagagcgg catctggcct actataccct ggtggatggc
     8821 aaagggaata tcattaagca ggatacattc aacatcattg gcaatgaccg gatgaaaacc
     8881 aactaccacg ataaactggc tgcaatcgag aaggatagag actcagctag gaaggactgg
     8941 aagaaaatca acaacattaa ggagatgaag gaaggctatc tgagccaggt ggtccatgag
     9001 attgcaaagc tggtcatcga atacaatgcc attgtggtgt tcgaggatct gaacttcggc
     9061 tttaagaggg ggcgctttaa ggtggaaaaa caggtctatc agaagctgga gaaaatgctg
     9121 atcgaaaagc tgaattacct ggtgtttaaa gataacgagt tcgacaagac cggaggcgtc
     9181 ctgagagcct accagctgac agctcccttt gaaactttca agaaaatggg aaaacagaca
     9241 ggcatcatct actatgtgcc agccggattc acttccaaga tctgccccgt gaccggcttt
     9301 gtcaaccagc tgtaccctaa atatgagtca gtgagcaagt cccaggaatt tttcagcaag
     9361 ttcgataaga tctgttataa tctggacaag gggtacttcg agttttcctt cgattacaag
     9421 aacttcggcg acaaggccgc taaggggaaa tggaccattg cctccttcgg atctcgcctg
     9481 atcaactttc gaaattccga taaaaaccac aattgggaca ctagggaggt gtacccaacc
     9541 aaggagctgg aaaagctgct gaaagactac tctatcgagt atggacatgg cgaatgcatc
     9601 aaggcagcca tctgtggcga gagtgataag aaatttttcg ccaagctgac ctcagtgctg
     9661 aatacaatcc tgcagatgcg gaactcaaag accgggacag aactggacta tctgattagc
     9721 cccgtggctg atgtcaacgg aaacttcttc gacagcagac aggcacccaa aaatatgcct
     9781 caggatgcag acgccaacgg ggcctaccac atcgggctga agggactgat gctgctgggc
     9841 cggatcaaga acaatcagga ggggaagaag ctgaacctgg tcattaagaa cgaggaatac
     9901 ttcgagtttg tccagaatag aaataacaaa aggccggcgg ccacgaaaaa ggccggccag
     9961 gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta tccctacgac
    10021 gtgcctgatt atgcataccc atatgatgtc cccgactatg cctaatctct tctcgagtca
    10081 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
    10141 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
    10201 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
    10261 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
    10321 tttgcgatcc ccttttcgtt tgcaagaaga aaggaaacaa tggagaagag atcatccatt
    10381 tgtatgtaaa ttttagtaaa cttgaagaaa tcactaacaa cttctcttac ttagggattc
    10441 tatgcaaaac cttgtaaatc atctgatgga ggactcgatt taatgaattg gaaggttgga
    10501 tttccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    10561 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    10621 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    10681 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    10741 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10801 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10861 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    10921 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10981 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    11041 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    11101 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    11161 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    11221 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    11281 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    11341 ggtgagcgtg gatctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    11401 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    11461 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    11521 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    11581 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    11701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    11821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    12001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
    12061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    12121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    12181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    12241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    12301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    12361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    12421 agcgaggaag cggaag
//
