LOCUS       Exported               13333 bp ds-DNA     circular SYN 15-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Boeke Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1487..1505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1545..1563
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..259
                     /label=T7
     misc_feature    314..1483
                     /label=fba1 promoter
     misc_feature    1487..1505
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    complement(1507..1512)
                     /label=BsaI
                     /note="BsaI recognition site GGTCTC"
     misc_feature    1538..1543
                     /label=BsaI
                     /note="BsaI recognition site GGTCTC"
     misc_feature    1545..1563
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    1568..1807
                     /gene="TEFt"
                     /product="TEF1 terminator of A. gossypii"
                     /label=TEF1 terminator of A. gossypii
     primer_bind     complement(1831..1847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1992..2447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2883..3677)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4297..5080
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     5232..5289
                     /label=YZ1036
     misc_feature    5262..5265
                     /label=CAGT
     promoter        5266..6007
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     misc_feature    5924..5948
                     /label=25bp
     CDS             6034..6054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6079..9975
                     /codon_start=1
                     /product="FnCpf1-NLS-3xHA tag"
                     /label=hudFnCpf1
                     /note="derived from Francisella novicida U112 (FnCpf1); 
                     optimized for expression in human"
                     /translation="SIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKD
                     YKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTI
                     KKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDE
                     ALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKD
                     KAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFN
                     TIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDK
                     LEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLT
                     DLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLA
                     LEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQAS
                     AEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIVP
                     LYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKK
                     NNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHS
                     THTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFY
                     REVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFD
                     ERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDKR
                     FTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIARGERHLAYYTLVDG
                     KGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVH
                     EIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTG
                     GVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEF
                     FSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTRE
                     VYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTEL
                     DYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNLV
                     IKNEEYFEFVQNRNN"
     primer_bind     8599..8622
                     /label=YZ1519
     misc_feature    8824..8826
                     /label=D917A mutation for dFnCpf1, WT =GAC
     CDS             9976..10023
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     10024..10091
                     /label=YZ1577
     misc_feature    10030..10062
                     /label=linker
     CDS             10063..10962
                     /codon_start=1
                     /label=Clr4 enzymatic SET domain
                     /translation="SKLDSYTHLSFYEKRELFRKKLREIEGPEVTLVNEVDDEPCPSLD
                     FQFISQYRLTQGVIPPDPNFQSGCNCSSLGGCDLNNPSRCECLDDLDEPTHFAYDAQGR
                     VRADTGAVIYECNSFCSCSMECPNRVVQRGRTLPLEIFKTKEKGWGVRSLRFAPAGTFI
                     TCYLGEVITSAEAAKRDKNYDDDGITYLFDLDMFDDASEYTVDAQNYGDVSRFFNHSCS
                     PNIAIYSAVRNHGFRTIYDLAFFAIKDIQPLEELTFDYAGAKDFSPVQSQKSQQNRISK
                     LRRQCKCGSANCRGWLFG"
     primer_bind     complement(10938..10974)
                     /label=YZ1578
     terminator      10975..11222
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11204..11260)
                     /label=YZ1037
     misc_feature    11230..11233
                     /label=TTTT
     promoter        11428..11532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11533..12393
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12564..13152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 aatacgactc actataggga aagcttgcat gcctgcaggt cgactctaga ggatcggccg
      301 gcccgcggcc gcacagtctc atactcttct agtcaagtct catcctcttc cactctctca
      361 attccttttt atcaatgttt cttcctcttg ttatgtacac aaacgatgat ctcttctctc
      421 acctcattgc tggactaata ttactcgtgg ccaagcgaaa cacaagtggt actagataac
      481 caaaaaatga taaagcattc aatcaatcaa accacttacg aaattcatac tcccgtaaac
      541 gaatgccggt cggacgcatg gaaaatcatc aatacgtgag agagctactt agttgtgcac
      601 tttgtatatg tccaaaaata cttttcattt cacatacctg cttgtgtaaa gacagtgatg
      661 aaatgcggtg caaagtgtct attcttacat tgatggtaaa gctaattcaa gcatgcttgg
      721 aagaagaatt gccttgcatt tcttgatcat ttgacccagg cgacaactat aaaataagaa
      781 tactaccgaa gatgcagatg gggtgtgtgg gcacaacacc atcgaaccga accgagacgg
      841 ccagactgtg ataacaaaag aaatgcatta gtttagttgg ctggttcaag ttggttgagt
      901 agaaaaatcc atcactcaaa ctgggaagta gggatggctg ggagaatgga gaagcgagac
      961 gagacgagac aagacaagag gagatgaaat gaaattaatg aaaagaaatg aatagaaatg
     1021 aaatagacat atgaatagta tagggcaggt tagatgtttg tatttataaa tgtttaatgc
     1081 ttaggtttgt atttatgttt acgttggtaa tgtttctttt gtttgtgttt gtgcatcggg
     1141 tgaacgagtt ggttaccgcc gccgccgctt ccttttgcct tgacacacac cacgcaacgt
     1201 gttacggttc ttgaacggaa ttgcgtactt cgatgcagag cgctatcggg gggattgggg
     1261 atggtaaatg gaagaaatca aaaacttctt ttgacgtcaa cccctaaaac aggggacggt
     1321 gattggtggt aaccacggac cggataggta tcgatatgca catcaggtga gaagtttttc
     1381 cgaggccaac tccggctcca tggtatataa tagagggttg cgcgctactg ttgcagtatc
     1441 atacttccaa aggtcgtata gaacacacac acaaattagc aaaatgaatt tctactgttg
     1501 tagatcgaga cctccctgca agaccatcta caaagttggt ctccaatttc tactgttgta
     1561 gattgagtca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1621 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1681 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1741 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1801 gtgtcgaagc ggccgcgcga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     1861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1981 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2401 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttagc ttgtgatatt
     2461 gacgaaactt tttgacatct aatttattct gttccaacac caatgtttat aaccaagttt
     2521 tatcttgttt gtctacatgg tattttacat tcatctacat acatctttca ttggctttgt
     2581 acatagttat cattacaagt ctaaaaaaat tcactctttt cttattcaat gtcaatccaa
     2641 gagaaaagat tgtggtaatg ttgtaggagc atgtttaata aattactata gcaaattact
     2701 ttttattccc aaggtgttta tctataatag ttaatatttt agtcgctaca taaaatttta
     2761 ccaaagagta cttgtatact aattctaaat gccttctgac ataaaacgcc taggaaaaca
     2821 aacgcaaaca aggcatcgac tttttcaata accaaccaaa aaaattttac attagtcttt
     2881 ttttaatgct gagaaagtct ttgctgatat gccttccaac cagcttctct atatctcttg
     2941 gcttcgacaa caggattacg accagctcca tagactccac gaccaacaat gatgatatcg
     3001 ctaccgcagt ttacaatcac ttcttcagga gtacgatatt gctgtcccag cccgtctcct
     3061 ttaacatcca agccgatacc aggggacata gttatgtagt cgctttgaag gttaggaaat
     3121 cgacgaccag ctataaagcc aaagcaaaaa tcggtatgct tctcaaacca ttctaaggtt
     3181 ttctctgtgt aggaaccagt agccaaagag cctttggaag acatttcagc caaaagcaag
     3241 agtccacgtc ccaaaggtaa accaacttct ttgaggcctt gtataatacc ctcgcctggc
     3301 actgtatggc aatttgtgat atgagcccaa gaagcaattt tgtacacacc agatgcatat
     3361 tgtagcttga cggtatttcc aatgtctgcg aatttgcgat cctcaaagat aagaaaacga
     3421 tgctttttac ctaaggccac cagtttttct accatatcct ggtcgaaatc ctcgacaacg
     3481 tcaatatgtg tcttgataac acagacatag ggtccaattt tatctaccaa ttctaagatt
     3541 tcggatttct tcgtcaaatc gaccgcgact gacaagttgc tttgcttttc ttccatcaaa
     3601 gccaacaatt ccttggcaat gggatttttc atcccctcag ctctagctga atagctttga
     3661 aatactctag catccataac tttgctttta aacctttaat ttcgatccaa gcaaaaaaga
     3721 ggttcttggt aggacaatac ggtaagaaaa cacgacatgt gcagagatgc cgacgaagca
     3781 tagttaaact gggatggtaa aatcaattaa gaatttataa agacaaaatt gtataagtct
     3841 ctaaaacatc ttaattatac ctcacagaac tatctaaaat atattcacaa agtgcaaaca
     3901 ttatcatgaa aaagaaccat tttaatttaa agcaagggca ttaaggctta tttacagaat
     3961 ttcttacttt tgtaaagatt ataaggctga ttatcttttt caccatgcca aaaattacac
     4021 aagatagaat ggatgtttga aattaaacgt gagtatacaa acaaatacac taggtaaatc
     4081 gaaacatttt tttctccatt aagtaacaaa ttcctattta gagaaagaat gctgagtaga
     4141 ttaaataatc tatacaaact tttttaacac aaatgcatac atatagccag tgggatttgt
     4201 agctagtcga tcgactctag aggaaattcg agtctaactc cttaaccact cggacatagt
     4261 gacttatctg acactcattg taaattaata tatatatagg cattttgttt agttaaaggt
     4321 acttaagtaa ttagtataaa cgaaccaatt ttataatcag gaagttaagt gaatggtagc
     4381 acatgtcgta aaaattgtga atttttattg aataatattt taaatacaag cctttctaga
     4441 ctaggtatac tcataaacat atatgagcaa aaggatagag gagattacat tgcatcttct
     4501 acaaatttat ttattgccct ttactgaaaa attaaataat gagtactaaa tgataaaaag
     4561 cgctcagtac aagaaagatt gcaaaaatat tgcattcttc atgaattaaa gttgcatata
     4621 aggcatattg aaagtaatag tactaaaaca gcagttagcg aaaattaata gaattatatt
     4681 cgcaagacaa ttgtgacatt aaattaaaaa attgtagaat ttttactatc ctctttaacg
     4741 ccatgagcct ttataaaaag gttaaattag ttttaacatt ctttttttga gtaagagttt
     4801 acaatttatc aaaacctgtg ttattatatt cattagtttc aatttattag catctagaga
     4861 aaaatcaatt ggcagttaca ttgttggaat ttatgaagaa aaagaatcta caacggagaa
     4921 taagttgctg atcgctttcc ctaaaattgt atattttgct gagcttattt tgacatttcg
     4981 ttgaagtttt ctctaattcg cattcatttt aagtaaaaca atgagaaata aaattacaaa
     5041 aaatacaaat taaaatacaa tttttagcta taattataga cgatgccctt gtatcccatt
     5101 ctgtctcgct tgcccctact ttttatcttt tatataccat aatgaacgct gccgctacta
     5161 accatacccc gattttacat ttcggactcc caaggacgta caaaatagaa aactatagaa
     5221 aaaaataatc agaaaatagc atgtcatctc tttgtaaaac gcagtgccct acaacaacta
     5281 agaaaatggc tatcatgcgg aagctggtga gaagaacagc atcgggacaa gggaaggaag
     5341 aacaaagaca aagaaaacaa aagaaagcaa ttgaaaacaa aacaaaacaa ttttcattcc
     5401 ttctcttatc attccttttc ttttcttttc tctcattcaa cgcactccat cgtatccgta
     5461 ttcctcttat tttttctctt tctctatatc catttctttc tctctaggtg tgtcctctct
     5521 ctctcttcaa tttctctact ccgcattcca acgcatcctt cccccaacct cccatttcct
     5581 ccttacggcc cgatagcgat cgtctttccc tcgctatcac tcgctaccgg cccctcctct
     5641 gcaccgtaac ctcctacgta tttaccatat cataaagttt tttccgacgc ttatcgctga
     5701 ccccctgtcg ccctcctatt ggcttccgga ttatcttctt gtccataagg tgatccatgc
     5761 ttcctgaaga ttcccgaaat gtgtccactt tggcggggaa tcattccatc cacttctttc
     5821 tctctcgctt tcctcattcg gcgctcccct tccgcgtctc attggtcttc cgctccgttt
     5881 ttgctttgcc gatgttactt ggggagaggt gcgataatcc tttcgcaaaa actcggtttg
     5941 acgcctccca tggtataaat agtgggtggt ggacaggtgc cttcgctttt ctttaagcaa
     6001 gagaattgct gcaggtcgac tctagagatg gctcctaaga agaagcggaa ggttggtatt
     6061 cacggggtgc ctgcggctag catctaccag gagttcgtca acaagtattc actgagtaag
     6121 acactgcggt tcgagctgat cccacagggc aagacactgg agaacatcaa ggcccgaggc
     6181 ctgattctgg acgatgagaa gcgggcaaaa gactataaga aagccaagca gatcattgat
     6241 aaataccacc agttctttat cgaggaaatt ctgagctccg tgtgcatcag tgaggatctg
     6301 ctgcagaatt actcagacgt gtacttcaag ctgaagaaga gcgacgatga caacctgcag
     6361 aaggacttca agtccgccaa ggacaccatc aagaaacaga ttagcgagta catcaaggac
     6421 tccgaaaagt ttaaaaatct gttcaaccag aatctgatcg atgctaagaa aggccaggag
     6481 tccgacctga tcctgtggct gaaacagtct aaggacaatg ggattgaact gttcaaggct
     6541 aactccgata tcactgatat tgacgaggca ctggaaatca tcaagagctt caagggatgg
     6601 accacatact ttaaaggctt ccacgagaac cgcaagaacg tgtactccag caacgacatt
     6661 cctacctcca tcatctaccg aatcgtcgat gacaatctgc caaagttcct ggagaacaag
     6721 gccaaatatg aatctctgaa ggacaaagct cccgaggcaa ttaattacga acagatcaag
     6781 aaagatctgg ctgaggaact gacattcgat atcgactata agactagcga ggtgaaccag
     6841 agggtctttt ccctggacga ggtgtttgaa atcgccaatt tcaacaatta cctgaaccag
     6901 tccggcatta ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg ggagaatacc
     6961 aagcgcaagg gaattaacga atacatcaat ctgtatagcc agcagatcaa cgacaaaact
     7021 ctgaagaaat acaagatgtc tgtgctgttc aaacagatcc tgagtgatac cgagtccaag
     7081 tcttttgtca ttgataaact ggaagatgac tcagacgtgg tcactaccat gcagagcttt
     7141 tatgagcaga tcgccgcttt caagacagtg gaggaaaaat ctattaagga aactctgagt
     7201 ctgctgttcg atgacctgaa agcccagaag ctggacctga gtaagatcta cttcaaaaac
     7261 gataagagtc tgacagacct gtcacagcag gtgtttgatg actattccgt gattgggacc
     7321 gccgtcctgg agtacattac acagcagatc gctccaaaga acctggataa tccctctaag
     7381 aaagagcagg aactgatcgc taagaaaacc gagaaggcaa aatatctgag tctggaaaca
     7441 attaagctgg cactggagga gttcaacaag cacagggata ttgacaaaca gtgccgcttt
     7501 gaggaaatcc tggccaactt cgcagccatc cccatgattt ttgatgagat cgcccagaac
     7561 aaagacaatc tggctcagat cagtattaag taccagaacc agggcaagaa agacctgctg
     7621 caggcttcag cagaagatga cgtgaaagcc atcaaggatc tgctggacca gaccaacaat
     7681 ctgctgcaca agctgaaaat cttccatatt agtcagtcag aggataaggc taatatcctg
     7741 gataaagacg aacacttcta cctggtgttc gaggaatgtt acttcgagct ggcaaacatt
     7801 gtccccctgt ataacaagat taggaactac atcacacaga agccttactc tgacgagaag
     7861 tttaaactga acttcgaaaa tagtaccctg gccaacgggt gggataagaa caaggagcct
     7921 gacaacacag ctatcctgtt catcaaggat gacaagtact atctgggagt gatgaataag
     7981 aaaaacaata agatcttcga tgacaaagcc attaaggaga acaaagggga aggatacaag
     8041 aaaatcgtgt ataagctgct gcccggcgca aataagatgc tgcctaaggt gttcttcagc
     8101 gccaagagta tcaaattcta caacccatcc gaggacatcc tgcggattag aaatcactca
     8161 acacatacta agaacgggag cccccagaag ggatatgaga aatttgagtt caacatcgag
     8221 gattgcagga agtttattga cttctacaag cagagcatct ccaaacaccc tgaatggaag
     8281 gattttggct tccggttttc cgacacacag agatataact ctatcgacga gttctaccgc
     8341 gaggtggaaa atcaggggta taagctgact tttgagaaca tttctgaaag ttacatcgac
     8401 agcgtggtca atcagggaaa gctgtacctg ttccagatct ataacaaaga tttttcagca
     8461 tacagcaagg gcagaccaaa cctgcataca ctgtactgga aggccctgtt cgatgagagg
     8521 aatctgcagg acgtggtcta taaactgaac ggagaggccg aactgtttta ccggaagcag
     8581 tctattccta agaaaatcac tcacccagct aaggaggcca tcgctaacaa gaacaaggac
     8641 aatcctaaga aagagagcgt gttcgaatac gatctgatta aggacaagcg gttcaccgaa
     8701 gataagttct ttttccattg tccaatcacc attaacttca agtcaagcgg cgctaacaag
     8761 ttcaacgacg agatcaatct gctgctgaag gaaaaagcaa acgatgtgca catcctgagc
     8821 attgcccgag gagagcggca tctggcctac tataccctgg tggatggcaa agggaatatc
     8881 attaagcagg atacattcaa catcattggc aatgaccgga tgaaaaccaa ctaccacgat
     8941 aaactggctg caatcgagaa ggatagagac tcagctagga aggactggaa gaaaatcaac
     9001 aacattaagg agatgaagga aggctatctg agccaggtgg tccatgagat tgcaaagctg
     9061 gtcatcgaat acaatgccat tgtggtgttc gaggatctga acttcggctt taagaggggg
     9121 cgctttaagg tggaaaaaca ggtctatcag aagctggaga aaatgctgat cgaaaagctg
     9181 aattacctgg tgtttaaaga taacgagttc gacaagaccg gaggcgtcct gagagcctac
     9241 cagctgacag ctccctttga aactttcaag aaaatgggaa aacagacagg catcatctac
     9301 tatgtgccag ccggattcac ttccaagatc tgccccgtga ccggctttgt caaccagctg
     9361 taccctaaat atgagtcagt gagcaagtcc caggaatttt tcagcaagtt cgataagatc
     9421 tgttataatc tggacaaggg gtacttcgag ttttccttcg attacaagaa cttcggcgac
     9481 aaggccgcta aggggaaatg gaccattgcc tccttcggat ctcgcctgat caactttcga
     9541 aattccgata aaaaccacaa ttgggacact agggaggtgt acccaaccaa ggagctggaa
     9601 aagctgctga aagactactc tatcgagtat ggacatggcg aatgcatcaa ggcagccatc
     9661 tgtggcgaga gtgataagaa atttttcgcc aagctgacct cagtgctgaa tacaatcctg
     9721 cagatgcgga actcaaagac cgggacagaa ctggactatc tgattagccc cgtggctgat
     9781 gtcaacggaa acttcttcga cagcagacag gcacccaaaa atatgcctca ggatgcagac
     9841 gccaacgggg cctaccacat cgggctgaag ggactgatgc tgctgggccg gatcaagaac
     9901 aatcaggagg ggaagaagct gaacctggtc attaagaacg aggaatactt cgagtttgtc
     9961 cagaatagaa ataacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa
    10021 aagggatccg gtagttccaa gcttggcggc agcggcggca gctccaaact tgactcttat
    10081 acccatctaa gtttttacga aaagagagag ctatttcgta agaaactgcg ggaaattgaa
    10141 ggacccgaag taacgcttgt caatgaagtt gacgatgaac cctgtccatc tttagatttt
    10201 caattcatta gccagtaccg cttaacacaa ggtgttatcc cgcctgatcc aaattttcaa
    10261 tctggatgca attgctcgtc ccttggcggt tgtgacctta acaatccttc tcgttgtgag
    10321 tgtttagatg atttggatga gccaactcat tttgcctatg atgcacaggg gcgggtccgt
    10381 gcagatacgg gtgccgttat atatgaatgc aactcttttt gttcttgttc aatggagtgt
    10441 cctaatcgcg ttgttcaacg tggtagaact cttccgcttg aaatatttaa aacaaaagaa
    10501 aaaggatggg gtgtacgttc gcttcgattt gcccctgctg gcacgtttat cacttgctat
    10561 ttaggagaag tcataacttc agcagaggcc gccaagcgtg acaaaaatta tgacgacgac
    10621 ggtattacct atttatttga tttagacatg tttgatgatg ctagcgagta tacagtggat
    10681 gctcagaact atggagatgt ctctcgtttt tttaaccact cctgttcacc taatatagct
    10741 atttattctg ctgttcggaa ccatggattt cgaaccattt atgatctcgc tttttttgcc
    10801 atcaaagaca ttcagcctct agaggagctt acatttgatt acgctggtgc aaaagatttc
    10861 tcacctgttc aatctcaaaa atctcagcaa aatagaattt caaaacttcg ccggcagtgc
    10921 aaatgtggtt ccgctaattg tcgtggctgg cttttcggtt aatctcttct cgagtcatgt
    10981 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
    11041 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
    11101 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
    11161 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
    11221 gcgatcccct tttcgtttgc aagaagaaag gaaacaatgg agaagagatc atccatttgt
    11281 atgtaaattt tagtaaactt gaagaaatca ctaacaactt ctcttactta gggattctat
    11341 gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa tgaattggaa ggttggattt
    11401 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    11581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    11641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11761 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    12001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    12061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    12121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    12181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    12241 gagcgtggat ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    12601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    12721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    12901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
    12961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    13021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    13081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    13141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    13201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    13261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13321 gaggaagcgg aag
//
