LOCUS       Exported               12401 bp ds-DNA     circular SYN 15-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Boeke Lab
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          682..700
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             279..321
                     /label=LacZ alpha ORF
     misc_feature    325..681
                     /label=rrk1 gene -1 to -358
     promoter        325..681
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_feature    682..700
                     /label=19bp Short Direct Repeat
                     /note="19bp direct repeat for CRISPR/FnCpf1 system
                     literature = FnCpf1: a novel and efficient genome editing 
                     tool for Saccharomyces cerevisiae"
     misc_feature    701..708
                     /label=Not1 cutting site
     misc_RNA        709..760
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             792..808
                     /label=LacZ alpha ORF(1)
     promoter        complement(871..889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1060..1515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(1951..2745)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      3365..4148
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     4300..4357
                     /label=YZ1036
     misc_feature    4330..4333
                     /label=CAGT
     promoter        4334..5075
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             5102..5122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5147..9043
                     /codon_start=1
                     /product="FnCpf1-NLS-3xHA tag"
                     /label=hudFnCpf1
                     /note="derived from Francisella novicida U112 (FnCpf1); 
                     optimized for expression in human"
                     /translation="SIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKD
                     YKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTI
                     KKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDE
                     ALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKD
                     KAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFN
                     TIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDK
                     LEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLT
                     DLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLA
                     LEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQAS
                     AEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIVP
                     LYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKK
                     NNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHS
                     THTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFY
                     REVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFD
                     ERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDKR
                     FTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIARGERHLAYYTLVDG
                     KGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVH
                     EIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTG
                     GVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEF
                     FSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTRE
                     VYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTEL
                     DYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNLV
                     IKNEEYFEFVQNRNN"
     misc_feature    7892..7894
                     /label=D917A mutation for dFnCpf1, WT =GAC
     CDS             9044..9091
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     9092..9159
                     /label=YZ1577
     misc_feature    9098..9130
                     /label=linker
     CDS             9131..10030
                     /codon_start=1
                     /label=Clr4 enzymatic SET domain
                     /translation="SKLDSYTHLSFYEKRELFRKKLREIEGPEVTLVNEVDDEPCPSLD
                     FQFISQYRLTQGVIPPDPNFQSGCNCSSLGGCDLNNPSRCECLDDLDEPTHFAYDAQGR
                     VRADTGAVIYECNSFCSCSMECPNRVVQRGRTLPLEIFKTKEKGWGVRSLRFAPAGTFI
                     TCYLGEVITSAEAAKRDKNYDDDGITYLFDLDMFDDASEYTVDAQNYGDVSRFFNHSCS
                     PNIAIYSAVRNHGFRTIYDLAFFAIKDIQPLEELTFDYAGAKDFSPVQSQKSQQNRISK
                     LRRQCKCGSANCRGWLFG"
     primer_bind     complement(10006..10042)
                     /label=YZ1578
     terminator      10043..10290
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10272..10328)
                     /label=YZ1037
     misc_feature    10298..10301
                     /label=TTTT
     promoter        10496..10600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10601..11461
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11632..12220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcgg atccactagt aacggccgcc
      301 agtgtgctgg aattcgccct tccttttgct tatgttggtg gtagttggca tgcgtagact
      361 gatgactagt cagcaaggag cgtagaacag tcacactcgt tatatatgtg cttccaagaa
      421 aactcaagaa tttaccatta gcaaacactt ttttgaaatg ttagacattt aaatgacgaa
      481 ggcatataga agctttgaat aggtgttgta aagtgttgat ttatgtgacg ctgagggtgc
      541 gcatgaaagg aatgttgggt cacgattatt aaacagtttg ctagcttgga cacttgagta
      601 ttggaagttg ttgaattcta aaaaactttc agttgatttg aatagttgct gttgccaaaa
      661 aacataacct gtaccgaaga aaatttctac tgttgtagat gcggccgccc tgtcaccgga
      721 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta
      781 gagtcgacct gaagggcgaa ttctgcagga attcgatatc aagcttatcg ataccgtcga
      841 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1021 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     1081 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1141 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1201 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1261 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1321 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1381 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1441 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1501 taacgtttac aatttagctt gtgatattga cgaaactttt tgacatctaa tttattctgt
     1561 tccaacacca atgtttataa ccaagtttta tcttgtttgt ctacatggta ttttacattc
     1621 atctacatac atctttcatt ggctttgtac atagttatca ttacaagtct aaaaaaattc
     1681 actcttttct tattcaatgt caatccaaga gaaaagattg tggtaatgtt gtaggagcat
     1741 gtttaataaa ttactatagc aaattacttt ttattcccaa ggtgtttatc tataatagtt
     1801 aatattttag tcgctacata aaattttacc aaagagtact tgtatactaa ttctaaatgc
     1861 cttctgacat aaaacgccta ggaaaacaaa cgcaaacaag gcatcgactt tttcaataac
     1921 caaccaaaaa aattttacat tagtcttttt ttaatgctga gaaagtcttt gctgatatgc
     1981 cttccaacca gcttctctat atctcttggc ttcgacaaca ggattacgac cagctccata
     2041 gactccacga ccaacaatga tgatatcgct accgcagttt acaatcactt cttcaggagt
     2101 acgatattgc tgtcccagcc cgtctccttt aacatccaag ccgataccag gggacatagt
     2161 tatgtagtcg ctttgaaggt taggaaatcg acgaccagct ataaagccaa agcaaaaatc
     2221 ggtatgcttc tcaaaccatt ctaaggtttt ctctgtgtag gaaccagtag ccaaagagcc
     2281 tttggaagac atttcagcca aaagcaagag tccacgtccc aaaggtaaac caacttcttt
     2341 gaggccttgt ataataccct cgcctggcac tgtatggcaa tttgtgatat gagcccaaga
     2401 agcaattttg tacacaccag atgcatattg tagcttgacg gtatttccaa tgtctgcgaa
     2461 tttgcgatcc tcaaagataa gaaaacgatg ctttttacct aaggccacca gtttttctac
     2521 catatcctgg tcgaaatcct cgacaacgtc aatatgtgtc ttgataacac agacataggg
     2581 tccaatttta tctaccaatt ctaagatttc ggatttcttc gtcaaatcga ccgcgactga
     2641 caagttgctt tgcttttctt ccatcaaagc caacaattcc ttggcaatgg gatttttcat
     2701 cccctcagct ctagctgaat agctttgaaa tactctagca tccataactt tgcttttaaa
     2761 cctttaattt cgatccaagc aaaaaagagg ttcttggtag gacaatacgg taagaaaaca
     2821 cgacatgtgc agagatgccg acgaagcata gttaaactgg gatggtaaaa tcaattaaga
     2881 atttataaag acaaaattgt ataagtctct aaaacatctt aattatacct cacagaacta
     2941 tctaaaatat attcacaaag tgcaaacatt atcatgaaaa agaaccattt taatttaaag
     3001 caagggcatt aaggcttatt tacagaattt cttacttttg taaagattat aaggctgatt
     3061 atctttttca ccatgccaaa aattacacaa gatagaatgg atgtttgaaa ttaaacgtga
     3121 gtatacaaac aaatacacta ggtaaatcga aacatttttt tctccattaa gtaacaaatt
     3181 cctatttaga gaaagaatgc tgagtagatt aaataatcta tacaaacttt tttaacacaa
     3241 atgcatacat atagccagtg ggatttgtag ctagtcgatc gactctagag gaaattcgag
     3301 tctaactcct taaccactcg gacatagtga cttatctgac actcattgta aattaatata
     3361 tatataggca ttttgtttag ttaaaggtac ttaagtaatt agtataaacg aaccaatttt
     3421 ataatcagga agttaagtga atggtagcac atgtcgtaaa aattgtgaat ttttattgaa
     3481 taatatttta aatacaagcc tttctagact aggtatactc ataaacatat atgagcaaaa
     3541 ggatagagga gattacattg catcttctac aaatttattt attgcccttt actgaaaaat
     3601 taaataatga gtactaaatg ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg
     3661 cattcttcat gaattaaagt tgcatataag gcatattgaa agtaatagta ctaaaacagc
     3721 agttagcgaa aattaataga attatattcg caagacaatt gtgacattaa attaaaaaat
     3781 tgtagaattt ttactatcct ctttaacgcc atgagccttt ataaaaaggt taaattagtt
     3841 ttaacattct ttttttgagt aagagtttac aatttatcaa aacctgtgtt attatattca
     3901 ttagtttcaa tttattagca tctagagaaa aatcaattgg cagttacatt gttggaattt
     3961 atgaagaaaa agaatctaca acggagaata agttgctgat cgctttccct aaaattgtat
     4021 attttgctga gcttattttg acatttcgtt gaagttttct ctaattcgca ttcattttaa
     4081 gtaaaacaat gagaaataaa attacaaaaa atacaaatta aaatacaatt tttagctata
     4141 attatagacg atgcccttgt atcccattct gtctcgcttg cccctacttt ttatctttta
     4201 tataccataa tgaacgctgc cgctactaac cataccccga ttttacattt cggactccca
     4261 aggacgtaca aaatagaaaa ctatagaaaa aaataatcag aaaatagcat gtcatctctt
     4321 tgtaaaacgc agtgccctac aacaactaag aaaatggcta tcatgcggaa gctggtgaga
     4381 agaacagcat cgggacaagg gaaggaagaa caaagacaaa gaaaacaaaa gaaagcaatt
     4441 gaaaacaaaa caaaacaatt ttcattcctt ctcttatcat tccttttctt ttcttttctc
     4501 tcattcaacg cactccatcg tatccgtatt cctcttattt tttctctttc tctatatcca
     4561 tttctttctc tctaggtgtg tcctctctct ctcttcaatt tctctactcc gcattccaac
     4621 gcatccttcc cccaacctcc catttcctcc ttacggcccg atagcgatcg tctttccctc
     4681 gctatcactc gctaccggcc cctcctctgc accgtaacct cctacgtatt taccatatca
     4741 taaagttttt tccgacgctt atcgctgacc ccctgtcgcc ctcctattgg cttccggatt
     4801 atcttcttgt ccataaggtg atccatgctt cctgaagatt cccgaaatgt gtccactttg
     4861 gcggggaatc attccatcca cttctttctc tctcgctttc ctcattcggc gctccccttc
     4921 cgcgtctcat tggtcttccg ctccgttttt gctttgccga tgttacttgg ggagaggtgc
     4981 gataatcctt tcgcaaaaac tcggtttgac gcctcccatg gtataaatag tgggtggtgg
     5041 acaggtgcct tcgcttttct ttaagcaaga gaattgctgc aggtcgactc tagagatggc
     5101 tcctaagaag aagcggaagg ttggtattca cggggtgcct gcggctagca tctaccagga
     5161 gttcgtcaac aagtattcac tgagtaagac actgcggttc gagctgatcc cacagggcaa
     5221 gacactggag aacatcaagg cccgaggcct gattctggac gatgagaagc gggcaaaaga
     5281 ctataagaaa gccaagcaga tcattgataa ataccaccag ttctttatcg aggaaattct
     5341 gagctccgtg tgcatcagtg aggatctgct gcagaattac tcagacgtgt acttcaagct
     5401 gaagaagagc gacgatgaca acctgcagaa ggacttcaag tccgccaagg acaccatcaa
     5461 gaaacagatt agcgagtaca tcaaggactc cgaaaagttt aaaaatctgt tcaaccagaa
     5521 tctgatcgat gctaagaaag gccaggagtc cgacctgatc ctgtggctga aacagtctaa
     5581 ggacaatggg attgaactgt tcaaggctaa ctccgatatc actgatattg acgaggcact
     5641 ggaaatcatc aagagcttca agggatggac cacatacttt aaaggcttcc acgagaaccg
     5701 caagaacgtg tactccagca acgacattcc tacctccatc atctaccgaa tcgtcgatga
     5761 caatctgcca aagttcctgg agaacaaggc caaatatgaa tctctgaagg acaaagctcc
     5821 cgaggcaatt aattacgaac agatcaagaa agatctggct gaggaactga cattcgatat
     5881 cgactataag actagcgagg tgaaccagag ggtcttttcc ctggacgagg tgtttgaaat
     5941 cgccaatttc aacaattacc tgaaccagtc cggcattact aaattcaata ccatcattgg
     6001 cgggaagttt gtgaacgggg agaataccaa gcgcaaggga attaacgaat acatcaatct
     6061 gtatagccag cagatcaacg acaaaactct gaagaaatac aagatgtctg tgctgttcaa
     6121 acagatcctg agtgataccg agtccaagtc ttttgtcatt gataaactgg aagatgactc
     6181 agacgtggtc actaccatgc agagctttta tgagcagatc gccgctttca agacagtgga
     6241 ggaaaaatct attaaggaaa ctctgagtct gctgttcgat gacctgaaag cccagaagct
     6301 ggacctgagt aagatctact tcaaaaacga taagagtctg acagacctgt cacagcaggt
     6361 gtttgatgac tattccgtga ttgggaccgc cgtcctggag tacattacac agcagatcgc
     6421 tccaaagaac ctggataatc cctctaagaa agagcaggaa ctgatcgcta agaaaaccga
     6481 gaaggcaaaa tatctgagtc tggaaacaat taagctggca ctggaggagt tcaacaagca
     6541 cagggatatt gacaaacagt gccgctttga ggaaatcctg gccaacttcg cagccatccc
     6601 catgattttt gatgagatcg cccagaacaa agacaatctg gctcagatca gtattaagta
     6661 ccagaaccag ggcaagaaag acctgctgca ggcttcagca gaagatgacg tgaaagccat
     6721 caaggatctg ctggaccaga ccaacaatct gctgcacaag ctgaaaatct tccatattag
     6781 tcagtcagag gataaggcta atatcctgga taaagacgaa cacttctacc tggtgttcga
     6841 ggaatgttac ttcgagctgg caaacattgt ccccctgtat aacaagatta ggaactacat
     6901 cacacagaag ccttactctg acgagaagtt taaactgaac ttcgaaaata gtaccctggc
     6961 caacgggtgg gataagaaca aggagcctga caacacagct atcctgttca tcaaggatga
     7021 caagtactat ctgggagtga tgaataagaa aaacaataag atcttcgatg acaaagccat
     7081 taaggagaac aaaggggaag gatacaagaa aatcgtgtat aagctgctgc ccggcgcaaa
     7141 taagatgctg cctaaggtgt tcttcagcgc caagagtatc aaattctaca acccatccga
     7201 ggacatcctg cggattagaa atcactcaac acatactaag aacgggagcc cccagaaggg
     7261 atatgagaaa tttgagttca acatcgagga ttgcaggaag tttattgact tctacaagca
     7321 gagcatctcc aaacaccctg aatggaagga ttttggcttc cggttttccg acacacagag
     7381 atataactct atcgacgagt tctaccgcga ggtggaaaat caggggtata agctgacttt
     7441 tgagaacatt tctgaaagtt acatcgacag cgtggtcaat cagggaaagc tgtacctgtt
     7501 ccagatctat aacaaagatt tttcagcata cagcaagggc agaccaaacc tgcatacact
     7561 gtactggaag gccctgttcg atgagaggaa tctgcaggac gtggtctata aactgaacgg
     7621 agaggccgaa ctgttttacc ggaagcagtc tattcctaag aaaatcactc acccagctaa
     7681 ggaggccatc gctaacaaga acaaggacaa tcctaagaaa gagagcgtgt tcgaatacga
     7741 tctgattaag gacaagcggt tcaccgaaga taagttcttt ttccattgtc caatcaccat
     7801 taacttcaag tcaagcggcg ctaacaagtt caacgacgag atcaatctgc tgctgaagga
     7861 aaaagcaaac gatgtgcaca tcctgagcat tgcccgagga gagcggcatc tggcctacta
     7921 taccctggtg gatggcaaag ggaatatcat taagcaggat acattcaaca tcattggcaa
     7981 tgaccggatg aaaaccaact accacgataa actggctgca atcgagaagg atagagactc
     8041 agctaggaag gactggaaga aaatcaacaa cattaaggag atgaaggaag gctatctgag
     8101 ccaggtggtc catgagattg caaagctggt catcgaatac aatgccattg tggtgttcga
     8161 ggatctgaac ttcggcttta agagggggcg ctttaaggtg gaaaaacagg tctatcagaa
     8221 gctggagaaa atgctgatcg aaaagctgaa ttacctggtg tttaaagata acgagttcga
     8281 caagaccgga ggcgtcctga gagcctacca gctgacagct ccctttgaaa ctttcaagaa
     8341 aatgggaaaa cagacaggca tcatctacta tgtgccagcc ggattcactt ccaagatctg
     8401 ccccgtgacc ggctttgtca accagctgta ccctaaatat gagtcagtga gcaagtccca
     8461 ggaatttttc agcaagttcg ataagatctg ttataatctg gacaaggggt acttcgagtt
     8521 ttccttcgat tacaagaact tcggcgacaa ggccgctaag gggaaatgga ccattgcctc
     8581 cttcggatct cgcctgatca actttcgaaa ttccgataaa aaccacaatt gggacactag
     8641 ggaggtgtac ccaaccaagg agctggaaaa gctgctgaaa gactactcta tcgagtatgg
     8701 acatggcgaa tgcatcaagg cagccatctg tggcgagagt gataagaaat ttttcgccaa
     8761 gctgacctca gtgctgaata caatcctgca gatgcggaac tcaaagaccg ggacagaact
     8821 ggactatctg attagccccg tggctgatgt caacggaaac ttcttcgaca gcagacaggc
     8881 acccaaaaat atgcctcagg atgcagacgc caacggggcc taccacatcg ggctgaaggg
     8941 actgatgctg ctgggccgga tcaagaacaa tcaggagggg aagaagctga acctggtcat
     9001 taagaacgag gaatacttcg agtttgtcca gaatagaaat aacaaaaggc cggcggccac
     9061 gaaaaaggcc ggccaggcaa aaaagaaaaa gggatccggt agttccaagc ttggcggcag
     9121 cggcggcagc tccaaacttg actcttatac ccatctaagt ttttacgaaa agagagagct
     9181 atttcgtaag aaactgcggg aaattgaagg acccgaagta acgcttgtca atgaagttga
     9241 cgatgaaccc tgtccatctt tagattttca attcattagc cagtaccgct taacacaagg
     9301 tgttatcccg cctgatccaa attttcaatc tggatgcaat tgctcgtccc ttggcggttg
     9361 tgaccttaac aatccttctc gttgtgagtg tttagatgat ttggatgagc caactcattt
     9421 tgcctatgat gcacaggggc gggtccgtgc agatacgggt gccgttatat atgaatgcaa
     9481 ctctttttgt tcttgttcaa tggagtgtcc taatcgcgtt gttcaacgtg gtagaactct
     9541 tccgcttgaa atatttaaaa caaaagaaaa aggatggggt gtacgttcgc ttcgatttgc
     9601 ccctgctggc acgtttatca cttgctattt aggagaagtc ataacttcag cagaggccgc
     9661 caagcgtgac aaaaattatg acgacgacgg tattacctat ttatttgatt tagacatgtt
     9721 tgatgatgct agcgagtata cagtggatgc tcagaactat ggagatgtct ctcgtttttt
     9781 taaccactcc tgttcaccta atatagctat ttattctgct gttcggaacc atggatttcg
     9841 aaccatttat gatctcgctt tttttgccat caaagacatt cagcctctag aggagcttac
     9901 atttgattac gctggtgcaa aagatttctc acctgttcaa tctcaaaaat ctcagcaaaa
     9961 tagaatttca aaacttcgcc ggcagtgcaa atgtggttcc gctaattgtc gtggctggct
    10021 tttcggttaa tctcttctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct
    10081 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
    10141 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
    10201 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
    10261 gttttgggac gctcgaaggc tttaatttgc gatccccttt tcgtttgcaa gaagaaagga
    10321 aacaatggag aagagatcat ccatttgtat gtaaatttta gtaaacttga agaaatcact
    10381 aacaacttct cttacttagg gattctatgc aaaaccttgt aaatcatctg atggaggact
    10441 cgatttaatg aattggaagg ttggatttcc aggtggcact tttcggggaa atgtgcgcgg
    10501 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10561 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10621 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10681 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10741 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10801 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    10861 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10921 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10981 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11041 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11101 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11161 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11221 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11281 gtttattgct gataaatctg gagccggtga gcgtggatct cgcggtatca ttgcagcact
    11341 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11401 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11461 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11521 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11581 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11641 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11701 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11761 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11821 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11881 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11941 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12001 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
    12061 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12121 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12181 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12241 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    12301 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    12361 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
