LOCUS       pJJD032_pFE_HCAII       3951 bp ds-DNA     circular     02-FEB-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(2056..2061)
                     /label="XbaI/SpeI Scar(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2544..2562
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    606..624
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     terminator      1556..1642
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(join(3520..3951,1..363))
                     /label="kanR"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     terminator      3395..3489
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1691..1773)
                     /label="ampReg_pSB1A2identity"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(2719..3307)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      1559..1602
                     /label="rrnB_T1 term terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1815..2438)
                     /label="tetR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1815..2438
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    581..599
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    686..1508
                     /label="HCA2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        2480..2508
                     /label="amp prom promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             664..675
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(2439..2543)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1710..1758
                     /label="Putative terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1651..1690
                     /label="UNS2-NheI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      2704..3323
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        581..654
                     /label="PLtet prom promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2465..2490
                     /label="pZ Strep F"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
ORIGIN
        1 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
       61 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
      121 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
      181 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
      241 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
      301 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
      361 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
      421 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
      481 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
      541 aaataggcgt atcacgaggc cctttcgtct tcacctcgag tccctatcag tgatagagat
      601 tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact gaccgaattc
      661 attaaagagg agaaaggtac cgtccaaaga ggagaaaggt accATGTCGC ATCACTGGGG
      721 TTATGGAAAA CACAACGGCC CGGAGCATTG GCACAAAGAT TTTCCCATTG CGAAGGGGGA
      781 ACGCCAATCC CCTGTCGACA TTGACACCCA CACGGCAAAG TATGACCCTA GCCTTAAACC
      841 ATTATCTGTA AGCTACGATC AAGCGACTTC CCTGCGTATC TTAAACAATG GGCATGCCTT
      901 TAATGTGGAG TTCGATGATA GCCAGGACAA GGCCGTTCTG AAAGGGGGTC CACTTGATGG
      961 CACCTACCGC CTGATCCAAT TCCACTTTCA CTGGGGGAGT CTGGACGGCC AAGGCTCTGA
     1021 GCATACAGTT GACAAGAAGA AGTACGCCGC AGAACTTCAC CTGGTTCATT GGAATACCAA
     1081 GTATGGAGAC TTCGGCAAAG CTGTGCAACA ACCAGACGGC TTGGCGGTGT TGGGTATCTT
     1141 CCTTAAAGTG GGATCTGCCA AACCTGGGTT ACAGAAGGTG GTGGATGTCC TTGATAGTAT
     1201 TAAGACTAAG GGAAAAAGTG CTGACTTTAC CAACTTTGAT CCGCGTGGTT TATTACCGGA
     1261 GAGCTTAGAT TATTGGACTT ATCCGGGTTC ATTAACCACA CCTCCCTTAC TGGAATGCGT
     1321 GACTTGGATT GTCCTGAAAG AGCCCATTTC CGTGTCAAGC GAGCAAGTCT TAAAGTTTCG
     1381 CAAACTTAAT TTCAATGGTG AAGGGGAACC AGAAGAACTT ATGGTGGACA ACTGGCGCCC
     1441 GGCGCAGCCC TTAAAAAATC GCCAGATCAA AGCGTCATTC AAAcaccatc accatcacca
     1501 ttgaagcgga attcctgcag cccgggggat cccatggtac gcgtgctaga ggcatcaaat
     1561 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1621 cgctctcctg agtaggacaa atccgccgcc GCTAGCAGTT CGTAGACGGA AACAAACGCA
     1681 GAATCCAAGC AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     1741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATGTCCACT GGGTTCGTGC CTTCATCCGG
     1801 ATATCCAGGA ACTGTTAAGA CCCACTTTCA CATTTAAGTT GTTTTTCTAA TCCGCATATG
     1861 ATCAATTCAA GGCCGAATAA GAAGGCTGGC TCTGCACCTT GGTGATCAAA TAATTCGATA
     1921 GCTTGTCGTA ATAATGGCGG CATACTATCA GTAGTAGGTG TTTCCCTTTC TTCTTTAGCG
     1981 ACTTGATGCT CTTGATCTTC CAATACGCAA CCTAAAGTAA AATGCCCCAC AGCGCTGAGT
     2041 GCATATAATG CATTCTCTAG TGAAAAACCT TGTTGGCATA AAAAGGCTAA TTGATTTTCG
     2101 AGAGTTTCAT ACTGTTTTTC TGTAGGCCGT GTACCTAAAT GTACTTTTGC TCCATCGCGA
     2161 TGACTTAGTA AAGCACATCT AAAACTTTTA GCGTTATTAC GTAAAAAATC TTGCCAGCTT
     2221 TCCCCTTCTA AAGGGCAAAA GTGAGTATGG TGCCTATCTA ACATCTCAAT GGCTAAGGCG
     2281 TCGAGCAAAG CCCGCTTATT TTTTACATGC CAATACAATG TAGGCTGCTC TACACCTAGC
     2341 TTCTGGGCGA GTTTACGGGT TGTTAAACCT TCGATTCCGA CCTCATTAAG CAGCTCTAAT
     2401 GCGCTGTTAA TCACTTTACT TTTATCTAAT CTAGACATac tcttcctttt tcaatattat
     2461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2521 aataaacaaa taggggttcc gcgTTTCCCT CGACAATTCG CGctagacct agggcgttcg
     2581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2881 ctttctccct tcgggaagcg tggcgctttc tcaTAgctca cgctgtaggt atctcagttc
     2941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3121 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3361 acgttaaggg attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc
     3421 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3481 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3541 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3601 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3661 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3721 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     3781 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     3841 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     3901 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc g
//