LOCUS       pfa31-sfgfp             3714 bp ds-DNA     circular     04-SEP-2019
DEFINITION  .
KEYWORDS    "marker:tetR"                                                      
FEATURES             Location/Qualifiers
     terminator      906..1000
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1995..2708
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     join(3703..3714,1..14)
                     /label="pZ Strep F"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    complement(2929..3011)
                     /label="ampReg_pSB1A2identity"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    1913..1931
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    complement(3294..3299)
                     /label="XbaI/SpeI Scar(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2733..2735
                     /label="Luc"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        1888..1961
                     /label="PLtet prom promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2797..2840
                     /label="rrnB_T1 term terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(3053..3676)
                     /label="tetR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     RBS             1971..1982
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(247..792)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        4..32
                     /label="amp prom promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1989..1991
                     /label="start"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1681..1783)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1021..1680)
                     /label="CAT (camR)"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             2715..2732
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    68..86
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      2794..2880
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1888..1906
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    2889..2928
                     /label="UNS2-NheI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(join(3677..3714,1..67))
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2948..2996
                     /label="Putative terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3053..3676
                     /label="pZS4Int match"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgTTT CCCTCGACAA TTCGCGctag acctagggat atattccgct tcctcgctca
      121 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
      181 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
      241 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
      301 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
      361 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      421 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      481 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      541 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      601 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      661 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      721 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
      781 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
      841 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gttactagtg
      901 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      961 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1021 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1081 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1141 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1201 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1261 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1321 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1381 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1441 accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg
     1501 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1561 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1621 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1681 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1741 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     1801 gatatcgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1861 acgaggccct ttcgtcttca cctcgagtcc ctatcagtga tagagattga catccctatc
     1921 agtgatagag atactgagca catcagcagg acgcactgac cgaattcatt aaagaggaga
     1981 aaggtaccAT GTCCgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2041 tcgagctgga cggcgacgta aacggccaca agttcagcgt gcgcggcgag ggcgagggcg
     2101 atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2161 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2221 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2281 gcaccatctc cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg
     2341 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2401 tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc acggccgaca
     2461 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacgtcgag gacggcagcg
     2521 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2581 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     2641 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2701 tgtacaagAG CGGTCACCAT CACCATCACC ATTAAAATGT AACTGTATTC AGCGATGACG
     2761 AAATTCTTAG CTATTGTAAT actctagagg catcaaataa aacgaaaggc tcagtcgaaa
     2821 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     2881 ccgccgccGC TAGCAGTTCG TAGACGGAAA CAAACGCAGA ATCCAAGCAA AAGGGAATAA
     2941 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     3001 ATCAGGGTTA TGTCCACTGG GTTCGTGCCT TCATCCGGAT ATCCAGGAAC TGTTAAGACC
     3061 CACTTTCACA TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA
     3121 AGGCTGGCTC TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA
     3181 TACTATCAGT AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA
     3241 ATACGCAACC TAAAGTAAAA TGCCCCACAG CGCTGAGTGC ATATAATGCA TTCTCTAGTG
     3301 AAAAACCTTG TTGGCATAAA AAGGCTAATT GATTTTCGAG AGTTTCATAC TGTTTTTCTG
     3361 TAGGCCGTGT ACCTAAATGT ACTTTTGCTC CATCGCGATG ACTTAGTAAA GCACATCTAA
     3421 AACTTTTAGC GTTATTACGT AAAAAATCTT GCCAGCTTTC CCCTTCTAAA GGGCAAAAGT
     3481 GAGTATGGTG CCTATCTAAC ATCTCAATGG CTAAGGCGTC GAGCAAAGCC CGCTTATTTT
     3541 TTACATGCCA ATACAATGTA GGCTGCTCTA CACCTAGCTT CTGGGCGAGT TTACGGGTTG
     3601 TTAAACCTTC GATTCCGACC TCATTAAGCA GCTCTAATGC GCTGTTAATC ACTTTACTTT
     3661 TATCTAATCT AGACATactc ttcctttttc aatattattg aagcatttat cagg
//